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Journal : Jurnal Agripet

Konsentrasi Superoxydase Dismutase (SOD) dan Malondialdehyda (MDA) Semen Cair Kambing Boer selama Pendinginan Menggunakan Pengencer Air Kelapa Muhammad Ade Salim; Muhammad Nur Ihsan; Nurul Isnaini; Trinil Susilawati
Jurnal Agripet Vol 22, No 1 (2022): Volume 22, No. 1, April 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i1.20073

Abstract

ABSTRACT. Lama simpan semen cair yang berbahan dasar pengencer air kelapa muda varietas kelapa hijau (C.Viridis) hanya mampu bertahan selama 3 hari pada suhu 50C akibat peroksidasi lipid yang menyebabkan kerusakan membran. Penelitian ini bertujuan untuk menganalisis pengaruh penggunaan pengencer air kelapa terhadap konsentrasi Superoxydase Dismutase (SOD) dan kadar Malondialdehyda (MDA) semen cair kambing Boer selama penyimpanan dingin. Durasi penelitian satu bulan bertempat di Laboratorium Fakultas Kedokteran dan Laboratorium Reproduksi Ternak, Unit Sumber Sekar, Fakultas Peternakan, Universitas Brawijaya, Malang. Metode penelitian yaitu eksperimen. Dikoleksi dari semen 3 pejantan Boer umur 3-5 tahun interval 1 minggu 1 kali menggunakan Vagina Buatan. Air kelapa muda varietas viridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Rancangan penelitian didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% Kuning Telur) dan P1 (air kelapa muda varietas viridis + 10% Kuning Telur) masing-masing diulang 3 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu konsentrasi SOD dan MDA. Hasil penelitian diperoleh untuk SOD P0 H1= 35,842 1,82 ; H3= 33,342 3,50 dan H8= 23,729 9,02 dalam ng/100 ug. Untuk P1 diperoleh rerata H1 =36,6762,19 ; H3= 36,5272,20 ; H8= 24,830 8,93 dalam ng/100ug. Kadar MDA P0 H1= 1,0720,2 ; H3= 1,218 0,4 dan H8= 1,439 0,3 dalam ng/100ug. Hasil P1 H1= 0,941 0,0 ; H3= 1,160 0,4 dan H8= 1,370 0,3. Hasil Anova Perlakuan tidak berpengaruh Nyata (P0,05) disemua hari simpan, tetapi konsentrasi SOD terbaik di P1 dibandingkan P0. Kesimpulan 1. Penggunaan air kelapa sebagai pengencer semen cair kambing Boer selama pendinginan tidak berdampak pada konsentrasi SOD dan MDA. 2. Peningkatan konsentrasi MDA dan Penurunan konsentrasi SOD disebabkan karena, rendahnya antioksidant flavonoid dan vitamin C.(Concentration of Superoxydase Dismutase (SOD) and Malondialdehyda (MDA) Goat BoerLiquid Semen during Cooling using Coconut Water Diluent)ABSTRAK. The shelf life of young coconut water thinner viridis for liquid semen quality Boer goat is only up to 3 days, because there is damage due to lipid peroxidation which causes damage to the spermatozoa membrane. This study aims to analyze the effect of using coconut water thinner on the concentration of Superoxydase Dismutase (SOD) and levels of Malondialdehyda in Boer goat liquid semen during cold storage. The duration of the research is one month at the Laboratory of the Faculty of Medicine and the Laboratory of Animal Reproduction, Sumber Sekar Unit, Faculty of Animal Husbandry, Brawijaya University, Malang. The research method is experimental. Collected from the semen of 3 Boer males aged 3-5 years 1 week interval using Artificial Vagina. Young viridis coconut water aged 5-7 months and tris aminomethane as a control. The research design was designed using a Randomized Block Design (RBD) with 2 treatments, namely T0 (tris aminomethane + 10% EY) and T1 (viridis young coconut water + 10% EY) each repeated 3 times. Data were analyzed by analysis of variance (Anova) with Genstat 18 software. The variables were SOD and MDA concentrations. The results obtained for SOD T0 D1 = 35.842 1.82; D3 = 33.342 3.50 and D8 = 23.729 9.02 in ng / 100 ug. For T1, the mean D1 = 36.676 2.19; D3 = 36,527 2.20; D8 = 24,830 8.93 in ng / 100ug. MDA levels T0 D1 = 1.072 0.2; D3 = 1.218 0.4 and D8 = 1.439 0.3 in ng / 100ug. Result of T1 H1 = 0.941 0.0; D3 = 1.160 0.4 and D8 = 1.370 0.3. The results of the ANOVA treatment had no significant effect (P 0.05) on all storage days, but the best SOD concentration was in T1 compared to T0. The conclusion is that 1. The use of coconut water as a liquid Boer goat semen thinner during cooling has no impact on the SOD and MDA concentrations. 2. Increased MDA concentrations and decreased SOD concentrations were due to low flavonoid and vitamin C antioxidants.
Evaluasi Tingkah Laku Sexual Kambing Kacang Jantan di Unit Pemukiman Transmigrasi (UPT) Desa Bina Gara Kabupaten Halmahera Timur Muhammad Ade Salim
Jurnal Agripet Vol 17, No 1 (2017): Volume 17, No. 1, April 2017
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v17i1.6571

Abstract

ABSTRAK. Keberhasilan IB kambing dipengaruhi salah satunya kualitas pejantan yang ditandai kemampuan menghasilkan semen dengan kualitas makroskopis dan mikroskopis tinggi. Kemampuan ini dipengaruhi oleh sexual behaviornya. Sebagai daerah transmigrasi yang berpotensi untuk pegembangan peternakan kambing, sekaligus banyak populasi kambing kacang di Halmahera Timur, sehingga desa Bina Gara menjadi lokasi sampel penelitian ini. Penelitian ini dilakukan dengan tujuan untuk mengevaluasi sexual behavior sebagai gambaran kinerja reproduksi kambing Kacang jantan. Materi yang digunakan yaitu 9 pejantan dan 5 betina yang dibagi dalam 3 kelompok umur masing-masing P1 (1,5 tahun), P2 (2,5 tahun) P3 (4 tahun). Metode yang digunakan yaitu survey dengan pemilihan sampel secara purposive sampling dengan variabel yaitu waktu reaksi, jumlah fals mounting, daya jepit, daya dorong dan kualitas ereksi. Data dianalisis deskriptif menggunakan software genstat 14.2. Hasil yang diperoleh yaitu waktu reaksi P2 terbaik = 1,070,13, fals mounting rata-rata 2x di semua kelompok umur, daya jepit dan daya dorong 3+ di semua kelompok umur dan skor kualitas ereksi 3+ pada semua kelompok umur. Kesimpulan penelitian ini yaitu tingkah laku sexual kambing kacang jantan di Unit Pemukiman Transmigrasi (UPT) Desa Bina Gara Kabupaten Halmahera Timur secara umum memiliki performans terbaik pada semua kelompok umur, ditandai score false mounting 2 kali, daya jepit +3 dan kualitas ereksi +3, sedangkan untuk waktu reaksi skor tertinggi yaitu pada kelompok umur 2,5 tahun.(The evaluation of sexual behavior on kacang buck (capra hircus) in transmigration settlement unit (TSU) Bina Gara Village East Halmahera District)ABSTRACT. The success of Artificial Insemination goat is related to quality service quality with high macroscopic and microscopic qualities. This ability is subject to sexual behavior. As a transmigration area that shifts for the development of goat farms, as well as many Kacang Buck populations in East Halmahera, so the village of Bina Gara became the sample location of this study. This study was conducted with the aim to raise sexual behavior as a description of the performance of Kacang Buck. The material used is 9 males and 5 females divided into 3 age groups of each P1 (1.5 years), P2 (2.5 years) P3 (4 years). The method used is survey with sample selection by purposive sampling with reaction time variabel, the amount of fals mounting, clamp power, thrust and erection quality. The data were analyzed descriptively using software genstat 14.2. The result is the best reaction time P2 = 1.07 0.13, False mounting averages 2x in all age groups; 3-clamp force and thrust in all age groups and 3+ erectile quality score in all age groups. This research is sexual behavior of goat beans in Transmigration Resettlement Unit (TRU) of Bina Gara Village of East Halmahera Regency has the best performance in all age group, cleanliness 2 times, power +3 clamp and erection quality +3, while for time The highest score reactions are in the 2.5 year age group.
Pengaruh Metode Thawing terhadap Kualitas Semen Beku Sapi Bali, Sapi Madura dan Sapi PO Muhammad Ade Salim; Trinil Susilawati; Sri Wahyuningsih
Jurnal Agripet Vol 12, No 2 (2012): Volume 12, No. 2, Oktober 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i2.197

Abstract

Effect of thawing technique to quality frozen semen spermatozoa in bali, madura and PO cattleABSTRACT. The experiment was conducted from November 2011 to January 2012 in BBIB Singosari. The study aims was to determine the effect of thawing techniques on the quality frozen bovine sperm Bali, Madura and PO cattle. This material used 90 frozen straw of three cattle from the three breed. The method is an experiment with three treatments and 10 replications for frozen to thawing in temperature water is 5 0C of duration 60 seconds, 15 0C duration 30 seconds and 37 0C duration 15 seconds. Variables observed in this study were sperm quality are motility, viability and abnormality. Data in percentage transformed in to arcus sinus were analyzed using Completely Randomized Block Design (CRBD) with replication as blocks of 10x. The results showed the thawing at 37 0C duration 15 seconds to highly significant effect (P0,01) viability and motility and significant effect (P0.05). The abnormalities, no significant effect (P0,05). Breed cattle not significant effect to quality. It can be concluded that thawing techniques impact the motility and viability in frozen bovine semen spermatozoa Bali, Madura and PO cattle. Thawing using 37 0C temperature water with duration of 15 seconds is the best method to obtain the quality of spermatozoa post thawing frozen sperm.