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All Journal ACTA VETERINARIA INDONESIANA Jurnal Ilmiah Mahasiswa Veteriner Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) Jurnal Kedokteran Hewan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Jurnal Medika Veterinaria Jurnal Agripet

. Darmawi

Fakultas Kedokteran Hewan Universitas Syiah Kuala, Jl. Tgk. H. Hasan Krueng Kale No. 4 Darussalam, Banda Aceh 23111, Indonesia.

Author-ID : 2480383

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Perbandingan Aktivitas Antelmintik Albendazole dan Levamisole terhadap Ascaridia galli secara In Vitro Ummu Balqis; Muhammad Hambal; . Darmawi; Abdul Harris; . Rasmaidar; Farida Athaillah; . Muttaqien; . Azhar; . Ismail; Razali Daud
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : IPB University

Penelitian ini meneliti aktivitas antelmintik albendazole dan levamisole terhadap hambatan motilitas, percepatan waktu paralisis, dan motilitas cacing Ascaridia galli dewasa secara in vitro. Sebanyak empat ekor cacing masing dibuat triplikat dalam NaCl 0,9% masing-masing dengan konsentrasi 15 mg/ml Albendazole, dan 0.6 mg/ml Levamisole. Motilitas cacing diamati pada interval 10, 20, 30, dan 40 jam. Paralisis dan kematian diamati pada tampilan tidak ada pergerakan badan pada bagian kepala dan ekor cacing. Hasil menunjukkan bahwa aktivitas albendazole dan levamisole terhadap mortalitas A. galli berturut-turut terjadi pada 40 dan 30 jam pasca inkubasi. Levamisole dapat menghambat motilitas A. galli pada jam ke 10 dan juga menyebabkan lebih awal paralisis pada 6,75 ± 0,50 jam pasca inkubasi. Kajian tersebut mengindikasikan bahwa aktivitas antelmintik levamisole lebih awal dibandingkan efek albendazole pada cacing A. galli.

ISOLASI DAN IDENTIFIKASI BAKTERI GRAM NEGATIF PADA AMBING KAMBING PERANAKAN ETAWA (PE) (Isolation and Identification of Gram Negative Bacteria on the Udder of Etawa Crossbred (PE) Goat) Septian Tri Mulyana Ginting; Teuku Zahrial Helmi; Darmawi Darmawi; Maryulia Dewi; Erina Erina; Razali Daud; Hennivanda Hennivanda
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

ABSTRAKPenelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Gram negatif pada ambing kambing peranakan etawa (PE). Sampel yang digunakan pada penelitian ini terdiri dari swab ambing dari 5 ekor kambing PE. Sampel dibiakkan pada media nutrient broth lalu diinkubasikan pada suhu 37°C selama 24 jam. Isolasi dan identifikasi dilakukan dengan metode Carter yang meliputi pengamatan morfologi koloni, pewarnaan Gram, penanaman pada media diferensial dan selektif serta uji biokimia. Hasil isolasi dan identifikasi pada 5 sampel swab ambing kambing PE didapatkan dua koloni bakteri Gram negatif yaitu Escherichia coli dan bakteri X yang belum teridentifikasi melalui uji-uji yang dilakukan. Berdasarkan hasil tersebut dapat disimpulkan bahwa bakteri Gram negatif pada ambing kambing PE adalah Escherichia coli dan bakteri X yang belum teridentifikasi.Kata kunci: kambing PE, ambing, bakteri Gram negatif. ABSTRACTThis study aimed to isolate and identify Gram-negative bacteria on udder of etawa crossbred (PE) goat. Swab samples were obtained from 5 PE goats udder. Swab samples were cultured in nutrient broth media and incubated at 37°C for 24 hours. Isolation and identification were performed by Carter method, followed by observation of colony morphology, Gram staining, cultured on differential and selective media, and biochemical tests were performed. The result showed that there were two colonies of Gram negative bacteria Escherichia coli and the other colony has not been identified yet with this tests. Therefore it can be concluded that the Gram-negative bacteria found on udder of PE goat were Escherichia coli and bacteria X that has not been identified by using this method.Keywords:etawa crossbred goat, udder, Gram-negative bacteria.

JUMLAH LEUKOSIT DAN ERITROSIT TIKUS PUTIH (Rattus norvegicus) YANG DIBERI EKSTRAK ETANOL BUNGA SIRSAK (Annona muricata L.) Zuraidawati Zuraidawati; Darmawi Darmawi; Sugito Sugito
Prosiding Seminar Nasional Biotik Vol 6, No 1 (2018): PROSIDING SEMINAR NASIONAL BIOTIK VI 2018
Publisher : Prosiding Seminar Nasional Biotik

Jumlah leukosit dan eritrosit merupakan dua parameter yang sangat penting untuk menilai kesehatan, dilihat dari kedua fungsinya masing-masing sangat penting bagi tubuh. Leukosit berfungsi menjaga pertahanan tubuh dan eritrosit berfungsi mengangkut O2 ke seluruh tubuh. Penelitian ini bertujuan untuk mengamati pengaruh pemberian ekstrak etanol bunga sirsak (Annona muricata L.) terhadap jumlah leukosit dan eritrosit tikus putih (Rattus norvegicus). Penelitian bersifat eksperimental, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial, terdiri dari 4 perlakuan dan 3 ulangan. Perlakuan KN merupakan kelompok kontrol negatif yang tidak diberi ekstrak etanol bunga sirsak, perlakuan P1, P2, dan P3 masing-masing diberi ekstrak etanol bunga sirsak dosis 0,18 gr/ekor/hari, 0,36 gr/ekor/hari, dan 0,72 gr/ekor/hari per oral selama 7 hari. Data hasil penelitian dianalisis menggunakan analysis of variance (ANOVA). Hasil penelitian menunjukkan pemberian ekstrak etanol bunga sirsak peroral dengan berbagai tingkatan dosis mampu meningkatkan jumlah leukosit tikus putih. Ekstrak etanol bunga sirsak dengan dosis 0,18/g/ekor/hari mampu meningkatkan jumlah leukosit dan eritrosit tikus putih, namun pada dosis 0,36/g/ekor/hari dan 0,72/g/ekor/hari jumlah eritrosit mengalami penurunan. Hasil uji statistik menunjukkan pemberian ekstrak etanol bunga sirsak peroral berpengaruh secara nyata (P<0,05) terhadap peningkatan jumlah leukosit tikus putih namun tidak berpengaruh nyata (P˃0,05) terhadap penurunan dan peningkatan jumlah eritrosit tikus putih.

The Development of Ascaridia galli Infective Eggs by In Vitro Culture Ummu Balqis; Darmawi D; Muhammad Hambal; Risa Tiuria
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli, embrionated eggs

RESPON ANTIBODI SERUM AYAM Breakel Silver TERHADAP VAKSIN AVIAN INFLUENZA Darmawi d; Muhammad Hambal
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Tujuan penelitian ini adalah menguji imunogenitas dari vaksin komersial Avian Influenza (AI) berdasarkan respon imunitas humoral ayam petelur terhadap AI. Sebanyak 20 ekor ayam petelur jenis breakel silver dibagi ke dalam dua kelompok masing-masing berjumlah 10 ekor. Pada kelompok pertama, ayam divaksinasi dengan vaksin komersial AI (H5N1). Pada kelompok kedua, ayam tidak divaksinasi. Sampel darah dari kedua kelompok ayam dikoleksi dan dievaluasi titer antibodinya dengan teknik Hemaglutination Inhibition (HI). Hasil penelitian ini menunjukkan bahwa vaksin komersial AI (H5N1) bersifat imunogen yang baik karena dapat memicu pembentukan respon humoral protektif ayam petelur yang ditandai dengan peningkatan titer antibodi serum ayam yang divaksin.

Goblet Cells Proliferation of Duodenum, Jejunum, and Ileum of Laying Hens Immunized with Protein of Excretory-Secretory of Ascaridia galli Ummu Balqis; Risa Tiuria; Bambang Pontjo Priosoeryanto; Darmawi D
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380µg of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehost’s defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells

RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli Darmawi D; Ummu Balqis; Risa Tiuria; Retno Damayanti Soejoedoeno; Fachriyan Hasymi Pasaribu; Muhammad Hambal; Razali Daud
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.

L3 Populations in Laying Hens Infected with 6,000 L2 of Ascaridia galli Darmawi D; Ummu Balqis; Risa Tiuria; Retno D. Soejoedono; Fachriyan H. Pasaribu
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae

ANTIBACTERIAL ACTIVITY AND HEMATOLOGICAL PROFILE OF RAT (Rattus norvegicus) DUE TO ADMINISTRATION OF ETHANOL EXTRACT OF SOURSOP FLOWERS (Annona muricata L.) AND Salmonella enteritidis INFECTION Zuraidawati Zuraidawati; Sugito Sugito; Darmawi Darmawi; Taufiq Taufiq
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

This study aimed to determine the antibacterial activity of soursop flower extract (Annona muricata L.) and hematological profile of rats (Rattus norvegicus) due to administration of soursop flower ethanol extract and Salmonella enteritidis infection. The concentrations of soursop flower ethanol extract used for the antibacterial activity test were 25%, 50%, 75%, 100%, with the antibiotic ampicillin 10 μg/disk was used s a positive control (PC) and dimethyl sulfoxide (DMSO) 10% as a negative control (NC). For examination of hematological features, 15 male rat aged two months were used. All rats were divided into 5 treatment groups, each consisting of three rats. The NC group was not given soursop flower ethanol extract and S. enteritidis infection. The PC group was not given soursop flower extract but was given S. enteritidis infection. Groups P1, P2, and P3 were given ethanol extracts of soursop flower at a dose of 0.18, 0.36, and 0.72 g/rat/day peroral for a week using gastric sonde. On the following day (after administration of soursop flower extract) the first blood drawing was performed. All rats, except NC group, were then infected with S. enteritidis intraperitonially at dose of 3x108 CFU/mL dose (0.5 mL McFarland). One week after being infected with S. enteritidis, a second blood drawing was performed. The results of the antibacterial activity test showed that there was no antibacterial activity was observed since no inhibition at various concentrations was formed. The administration of soursop flower extract at various dosage levels was able to maintain the number of leukocytes but reduced the number of erythrocytes, hemoglobin concentration, hematocrit value and the number of platelets in rats; whereas S. enteritidis infection decreased all the hematologic features of lab rats.

Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province Irma Dewiyanti; Darmawi Darmawi; Zainal Abidin Muchlisin; Teuku Zahrial Helmi; Iko Imelda Arisa; Cut Nanda Defira; Fitriyani Fitriyani; Sawva Yura
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

Co-Authors . Azhar . Darniati . Ismail . Muttaqien . Nurliana . Rasmaidar Abdul Harris Abdul Harris Abdullah Hamzah Abdullah Hamzah Ali Djamhuri Anak Agung Gede Sugianthara Azhar Azhar Azhar Azhar Bambang Pontjo Priosoeryanto Basri Gani Cut Nanda Defira Denny Irmawati Hasan Eliawardani Eliawardani Eliawardani Eliawardani Erina Erina Erina Erina Etriwati Etriwati Fachriyan H. Pasaribu Fachriyan Hasymi Pasaribu Fakhrurrazi Fakhrurrazi Farida Athaillah Hendri Hermawan Adinugraha HENNIVANDA HENNIVANDA Iko Imelda Arisa Irma Dewiyanti M. Nur Salim Mahdi Abrar Maryam Maryam Maryulia Dewi Maryulia Dewi Meliyantika Angreini Muhammad Hambal Mulyadi Mulyadi Muslina Muslina Nazaruddin Nazaruddin Razali Daud Razali Daud Retno D. Soejoedono Retno Damayanti Soejoedono Rinidar Rinidar Risa Tiuria Samadi Samadi Sawva Yura Septian Tri Mulyana Ginting Siti Aisyah Siti Rani Ayuti Sugito Sugito T. Zahrial Helmi Taufiq Taufiq Teuku Zahrial Helmi Ummu Balqis Winaruddin Winaruddin Yurliasni Yurliasni Zainal Abidin Muchlisin Zakiyah Heryawati Manaf Zuraidawati Zuraidawati Zuraidawati Zuraidawati

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