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Perbandingan Aktivitas Antelmintik Albendazole dan Levamisole terhadap Ascaridia galli secara In Vitro Ummu Balqis; Muhammad Hambal; . Darmawi; Abdul Harris; . Rasmaidar; Farida Athaillah; . Muttaqien; . Azhar; . Ismail; Razali Daud
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : IPB University

Penelitian ini meneliti aktivitas antelmintik albendazole dan levamisole terhadap hambatan motilitas, percepatan waktu paralisis, dan motilitas cacing Ascaridia galli dewasa secara in vitro. Sebanyak empat ekor cacing masing dibuat triplikat dalam NaCl 0,9% masing-masing dengan konsentrasi 15 mg/ml Albendazole, dan 0.6 mg/ml Levamisole. Motilitas cacing diamati pada interval 10, 20, 30, dan 40 jam. Paralisis dan kematian diamati pada tampilan tidak ada pergerakan badan pada bagian kepala dan ekor cacing. Hasil menunjukkan bahwa aktivitas albendazole dan levamisole terhadap mortalitas A. galli berturut-turut terjadi pada 40 dan 30 jam pasca inkubasi. Levamisole dapat menghambat motilitas A. galli pada jam ke 10 dan juga menyebabkan lebih awal paralisis pada 6,75 ± 0,50 jam pasca inkubasi. Kajian tersebut mengindikasikan bahwa aktivitas antelmintik levamisole lebih awal dibandingkan efek albendazole pada cacing A. galli.

Observasi Kesembuhan Distant Skin Flap yang dirawat dengan Dry Dressing dan Moist Dressing Erwin Erwin; Syafruddin Syafruddin; Fadrial Karmil; Sugito Sugito; Razali Daud; Roslizawaty Roslizawaty; Zainuddin Zainuddin; Roby Luksmana
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (848.855 KB)

Distant flap adalah teknik bedah untuk merekonstruksi luka yang jauh dari sumber flaps berasal. Penelitian ini bertujuan mengetahui perbedaan kesembuhan luka distant skin flap dengan perawatan dry dressing dan moist dressing secara subjektif dan objektif. Penelitian ini menggunakan 6 ekor kucing lokal jantan berumur 1-2 tahun dengan berat badan 2-3 kg, dibagi menjadi 2 kelompok perlakuan. Semua kucing dibuat luka dengan ukuran 2x2 cm pada kulit metacarpus, luka ditangani dengan teknik distant skin flap yang berasal dari lateral thoraks sebagai sumber flaps. Distant skin flap dirawat dengan dry dressing menggunakan kasa steril (K-I) dan moist dressing menggunakan sofratulle® (K-II). Pengamatan subjektif kesembuhan distant skin flap pada hari ke-3, 6, 9 dan 12 pasca bedah, sedangkan uji pendarahan dan pengamatan objektif pada hari ke-18 pasca bedah. Data kuantitatif pengamatan subjektif dan objektif dianalisis menggunakan analisis varian multivariate dan post hoc test Duncan dengan bantuan perangkat lunak SPSS versi 25. Hasil pengamatan subjektif menunjukkan kesembuhan luka distant skin flap yang dirawat dengan moist dressing lebih baik dari pada dry dressing. Hari ke-12 warna kulit flaps kembali sama dengan kulit sekitar, respon nyeri berkurang, dan pertumbuhan rambut lebih cepat. Pengamatan objektif menunjukkan waktu absorbsi NaCl 0,9% dan efek obat lebih cepat pada kelompok moist dressing. Kesembuhan distant skin flap yang dirawat dengan moist dressing lebih cepat dibandingkan dengan dry dressing.

Determination of Gestational Age and Observation of Kacang Goat Fetal Development during 60 Days of Pregnancy by Using Transcutaneous Ultrasonography Arman Sayuti; Khairiah Khairiah; Tongku Nizwan Siregar; Juli Melia; Syafruddin Syafruddin; Erdiansyah Rahmi; Herrialfian Herrialfian; Mahdi Abrar; Budianto Panjaitan; Razali Daud
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study was aimed to observe correlation between gestational age and fetal growth of Kacang goat for 60 days after mating with transcutaneous ultrasonography visualization. Three female goats of productive age with normal estrus cycle and one male goat were used in this study. The female goats were synchronized with double injection of prostaglandin F2á (PGF2á) intramuscularly with dosage of 1 mL for each female goat with 11 days’ time interval. Observation of oestrus was conducted using male goat after being synchronized. When the sign of estrus were prominent, the female goats were naturally mated. The result was pregnant positive for one female goat. Detection of early pregnancy was observed on the 24th days after mating, with the result of 6.77 mm length embryo by isoechogenic visualization. On 35th days of pregnancy, fetal could be seen clearly in isoechogenic to hyperechogenic visualization with head diameter and fetal length were 17.4 mm and 36.2 mm, consecutively. The observation also found the placentom. On day 45 of pregnancy, head diameter and fetal length were 21.8 mm and 40.6 mm. Later on day 49 of pregnancy, the size of gestational saccus was 44.1 mm, with head diameter of 25 mm, and average placentom diameter size of 12.4 mm. On day 53 of pregnancy, head diameter was 25.2 mm with fetal length of 63.6 mm and placentom diameter of 15 mm. On day 56 of pregnancy, vertebrae of fetal were observed using hyperechogenic visualization and placentom was measured 17.9 mm in diameter. On day 60 of pregnancy, we observed that the fetal length was 79.8 mm and the organs such as eyes, heart, liver, os costae, and fetal extremities, could be observed clearly. The size of developing fetal and organs would grow along with the addition of gestational age.

Keragaman Lalat Penghisap Darah Sebagai Vektor Potensial Trypanosoma Evansi di Daerah Pegunungan dan Pesisir di Kabupaten Aceh Besar Raja renca; Yudha Fahrimal; Razali Daud
JURNAL ILMIAH MAHASISWA VETERINER Vol 7, No 1 (2022): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v7i1.6857

ABSTRAKPenelitian bertujuan mengindentifikasi keragaman jenis lalat penghisap darah sebagai vektor potensial T. evansi di pegunungan dan pesisir pantai. Koleksi sampel dilakukan pada peternakan yang ada di kecamatan Saree dan Jantho untuk mewakili daerah pegunungan, dan Kecamatan Krueng Raya, dan Pekan Bada untuk mewakili daerah pesisir. Dalam penelitian ini masing-masing lokasi dipasang perangkap lalat tipe NZ1 trap yang ditempatkan di sekitar kandang berjarak sekitar ± 10 m dari kandang selama 24 jam dan menggunakan tangguk serangga (sweepnet) yang dilakukan pada daerah dalam kandang. Lalat dieuthanasi menggunakan ethanol 70%. Seluruh sampel yang diperoleh dari setiap lokasi diidentifikasi menggunakan kunci identifikasi. Hasil penelitian yang dilakukan pada peternakan di Kabupaten Aceh Besar diperoleh 3 (tiga) jenis lalat penghisap darah yaitu Haematobia exigua, Stomoxys calcitran, dan Tabanus sp. Spesies lalat yang mendominasi adalah Haematobia exigua.Kata kunci : Haematobia exigua, Stomoxys calcitran, Tabanus spABSTRACTThis study aims to identify the diversity of blood-sucking flies as the potential vector of T. evansi in the montainous and coastal areas. The collection of samples were conducted on the farms in saree and jantho sub-disricts to represent montainous areas, and krueng Raya sub-districts and Pekan Bada to represent coastal areas. In this study each location the trap flies type NZ-1 trap was installed which placed around the cage about 10 m away from the cage for 24 hours and using the insect net (Sweepnet) which done on the inside of the cage. The fly was euthanized using 70% ethanol. All samples obtained from each location identified using identification keys. The results of this study conducted, in the livestock in Aceh Besar district obtained 3 sp blood-sucking flies that Haematobia exigua, Stomoxys calcitran and Tabanus sp. The species of blood-sucking flies that dominate is Haematobia exigua.Keywords: Haematobia exigua, Stomoxys calcitran, Tabanus sp

The Use of Sour Soup (Annona murricata) Seed Powder as Acaricide on Cow and Goat Yudha Fahrimal; Razali Daud; Adi Chandra; Syauki Iqbal; Roslizawaty Roslizawaty
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

This research was aimed to study curative effect of sour soup seed powder on cattle invested with ticks and goat infected with scabies. This study was using 12 cattle invested with ticks and 12 goats with scabies. The cattle divided into 4 groups (S1, S2, S3 and S4) while goats were divided into 3 groups (K1, K2, and K3) equally. For cattle with ticks group S1 received water (control group), while group S2, S3, and S4 received 1%, 5%, and 10% sour soup powder respectively. Ticks that fell to the ground and not engorged were collected and identified. Statistical analysis showed that all concentrations of sour soup were effective in paralyzing and or killing ticks of the genera Boophilus sp. and Dermacentor sp. but were not effective against Rhipicepalus sp. For goats with scabies, groups K1, K2, and K3 received 1, 5 and 10% sour soup powder respectively mixed with water applied to whole area of infected and uninfected skin surrounding infected area. Number of mites per cm2 before and after treatment was counted. Statistical analysis showed that 1, 5, and 10% sour soup powder effective in reducing the number of scabies mites on day 1 and 7 after treatment and were significantly different from those number of mites before treatment (P0.01). Statistical analysis also showed that no significant difference among concentration of sour soup seed powder in decreasing the number of mites (P0.05).

The Effect of Vitamin E Supplementation on the Histopathological Changes of The Hearts Mice Given High Fat Diet Razali Daud
Jurnal Kedokteran Hewan Vol 1, No 1 (2007): March
Publisher : Universitas Syiah Kuala

The aim of this study was to determine the protective effect of vitamin E on mice heart. Twentyfivemalemiceweighing±40gattheageof3monthswereused.Themicewereadaptedforaweekandfedbasaldiet.Themicewerefednormaldietascontrol(group1),highfat+50IUVitaminE(groupII);highfat + 100 IU Vitamin E (group III) high fat + 200 IU Vitamin E (group IV); high fat + 400 IUVitamin E (Group V). Diet was given about 10% body weight, water was given ad libitum every day. After 3 months, all animal were then killed. The hearts were collected for routine histopathologicalexamination. The result indicate that lesions in the heart consist of swollen eosinophilic hyalinization ofcytoplasm, vacuolization, and necrosis myocardium (cardiomiopathy) in all mice receiving high fat dietwith less than 400 IU Vitamin E.Keywords: high fat diet, vitamin E, cardiovascular diseases

RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli Darmawi D; Ummu Balqis; Risa Tiuria; Retno Damayanti Soejoedoeno; Fachriyan Hasymi Pasaribu; Muhammad Hambal; Razali Daud
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.

The Use of Sour Soup (Annona murricata) Seed Powder as Acaricide on Cow and Goat Yudha Fahrimal; Razali Daud; Adi Chandra; Syauki Iqbal; Roslizawaty Roslizawaty
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9797

This research was aimed to study curative effect of sour soup seed powder on cattle invested with ticks and goat infected with scabies. This study was using 12 cattle invested with ticks and 12 goats with scabies. The cattle divided into 4 groups (S1, S2, S3 and S4) while goats were divided into 3 groups (K1, K2, and K3) equally. For cattle with ticks group S1 received water (control group), while group S2, S3, and S4 received 1%, 5%, and 10% sour soup powder respectively. Ticks that fell to the ground and not engorged were collected and identified. Statistical analysis showed that all concentrations of sour soup were effective in paralyzing and or killing ticks of the genera Boophilus sp. and Dermacentor sp. but were not effective against Rhipicepalus sp. For goats with scabies, groups K1, K2, and K3 received 1, 5 and 10% sour soup powder respectively mixed with water applied to whole area of infected and uninfected skin surrounding infected area. Number of mites per cm2 before and after treatment was counted. Statistical analysis showed that 1, 5, and 10% sour soup powder effective in reducing the number of scabies mites on day 1 and 7 after treatment and were significantly different from those number of mites before treatment (P0.01). Statistical analysis also showed that no significant difference among concentration of sour soup seed powder in decreasing the number of mites (P0.05).

The Effect of Vitamin E Supplementation on the Histopathological Changes of The Hearts Mice Given High Fat Diet Razali Daud
Jurnal Kedokteran Hewan Vol 1, No 1 (2007): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i1.3121

The aim of this study was to determine the protective effect of vitamin E on mice heart. Twentyfivemalemiceweighing±40gattheageof3monthswereused.Themicewereadaptedforaweekandfedbasaldiet.Themicewerefednormaldietascontrol(group1),highfat+50IUVitaminE(groupII);highfat + 100 IU Vitamin E (group III) high fat + 200 IU Vitamin E (group IV); high fat + 400 IUVitamin E (Group V). Diet was given about 10% body weight, water was given ad libitum every day. After 3 months, all animal were then killed. The hearts were collected for routine histopathologicalexamination. The result indicate that lesions in the heart consist of swollen eosinophilic hyalinization ofcytoplasm, vacuolization, and necrosis myocardium (cardiomiopathy) in all mice receiving high fat dietwith less than 400 IU Vitamin E.Keywords: high fat diet, vitamin E, cardiovascular diseases

RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli Darmawi D; Ummu Balqis; Risa Tiuria; Retno Damayanti Soejoedoeno; Fachriyan Hasymi Pasaribu; Muhammad Hambal; Razali Daud
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.929

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.

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