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Polymerase chain reaction (PCR) primer design to identify SNP rs7901695 transcription factor 7 like 2 (TCF7L2) Elsa Badriyya; Afifatul Achyar
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371122575-0-00

ABSTRACT. Transcription Factor 7 Like 2 (TCF7L2) is a gene that produces a protein that controls the expression and function of several incretin hormones. One of the primary activities of incretin hormones is to increase insulin production, which is important in glucose and energy homeostasis. Single Nucleotide Polymorphism (SNP) in the TCF7L2 gene is reported to be association with Type II Diabetes Mellitus (T2DM). T2DM is featured by persistent hyperglycemia because of the decrease in insulin production, insulin resistance, or both. In patients with diabetes mellitus, chronic hyperglycemia can damage organ systems and cause metabolic abnormalities. SNP rs7901695 TCF7L2 gene has been linked to T2DM in a number of places, including South Asia, Iceland, and the United States. The polymorphism at location 112.994.329 from nucleotide Timine (T) to Cytosine (C) enables the recognition of SNP rs7901695. Polymerase Chain Reaction (PCR) was used to identify the polymorphism. The objective of this research was to generate a precise primer for the PCR recognition of the SNP rs7901695 in the TCF7L2 gene. The research's methodology comprises DNA isolation, primer designing with Geneious, target amplification with PCR, and DNA sequencing for bioinformatic analysis. As a result of the study, four primers for the SNP rs7901695 TCF7L2 gene have been developed. The reaction obtained two fragments, sized 177 and 367 bp. The primers used were rs7901695-F, rs7901695-R, rs7901695-F(C), and rs7901695-R(T), which were used to detect the T allele. ABSTRAK. Transcription Factor 7 Like 2 (TCF7L2) adalah gen yang menghasilkan protein yang mengontrol ekspresi dan fungsi beberapa hormon inkretin. Salah satu aktivitas utama hormon inkretin adalah meningkatkan produksi insulin, yang penting dalam homeostasis glukosa dan energi. Single Nucleotide Polymorphism (SNP) pada TCF7L2 gene dilaporkan adanya hubungan dengan Diabetes Mellitus Tipe II (DMT2). DMT2 ditandai dengan hiperglikemia persisten karena penurunan produksi insulin, resistensi insulin, atau keduanya. Pada penderita diabetes melitus, hiperglikemia kronis dapat merusak sistem organ dan kelainan metabolisme. Gen SNP rs7901695 TCF7L2 dilaporkan memiliki asosiasi dengan DMT2 di sejumlah daerah, termasuk Asia Selatan, Islandia, dan Amerika Serikat. SNP rs7901695 ditandai dengan polimorfisme pada lokasi 112.994.329 dari nukleotida Timin (T) menjadi Sitosin (C). Metode yang digunakan untuk mengidentifikasi polimorfisme adalah Polymerase Chain Reaction (PCR). Penelitian ini bertujuan untuk menghasilkan primer yang tepat untuk pengenalan PCR terhadap SNP rs7901695 pada gen TCF7L2. Metodologi penelitian meliputi isolasi DNA, perancangan primer dengan aplikasi Geneious, amplifikasi target dengan PCR, dan sekuensing DNA untuk analisis bioinformatik. Sebagai hasil penelitian, empat primer untuk deteksi gen SNP rs7901695 TCF7L2 telah dikembangkan. Dua fragmen, berukuran 177 dan 367 bp, diperoleh dari reaksi tersebut. Primer yang digunakan adalah rs7901695-F, rs7901695-R, rs7901695-F(C), dan rs7901695-R(T), yang digunakan untuk mendeteksi alel T.

PPUPIK â€œSIPODEHâ€ Jahe Merah Instan Peluang Bisnis Mahasiswa Departemen Biologi S. Syamsurizal; Sa`diatul Fuadiyah; Elsa Badriyya
Pelita Eksakta Vol 6 No 1 (2023): Pelita Eksakta Vol. 6 No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol6-iss1/201

Ginger (Zingiber officinale) is an Indonesian spice plant which is widely used as traditional medicine. Another type of ginger that has more properties and a stronger spicy taste, namely red ginger. Red ginger is predicted to be able to inhibit the infection process of the SARS-CoV-2 virus in human host cells. Red ginger can increase the body's immunity, increase IgM levels and reduce the circulation of pro-inflammatory cytokines. The PPUPIK service team in collaboration with the HMJ Biology KWU Unit has developed the instant red ginger â€œSipodehâ€ product well. Currently we are expanding the marketing system. Some of the good practices that have been carried out are training the PPUPIK team to understand the basic concepts of marketing, studying marketing strategies, making simple bookkeeping. The results of this product marketing training were directly applied to various national scale activities by participating in bazaars at POMNAS at UNP. At the provincial level the "SIPODEH" team played an active role in the West Sumatra Malagak event sponsored by the West Sumatra regional government together with West Sumatra HIPMI. Participating in national and provincial scale activities succeeded in introducing "Sipodeh" instant red ginger well to consumers.

Peningkatan Pengetahuan Masyarakat tentang Penggunaan Obat yang Rasional melalui Edukasi Pengenalan Obat Dian Ayu Juwita; Elsa Badriyya; Lailaturrahmi Lailaturrahmi
Warta Pengabdian Andalas Vol 30 No 3 (2023)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.30.3.423-428.2023

One of the factors influencing the level of drug usage is the level of knowledge. Knowledge is an essential aspect in shaping concrete actions. In society, various issues related to drug usage often arise due to a lack of understanding and incorrect behaviours regarding drug usage. Educating the public about the selection and rational use of drugs is compulsary cause of that. This activity was carried out on May 7, 2023, at Jondul Housing, Padang City. The education method employed was a lecture delivered to 35 participating mothers. This method has proven effective for all segments of society, regardless of their educational background. The general public showed high enthusiasm in participating and actively engaged in interactions, asking questions, and discussing with the speaker. The conclusion drawn from this activity is that education and outreach enhanced the public knowledge about the rational use of drugs.

Design of primer Ipomoea batatas chloroplast gene matK S. Syamsurizal; Ardi Ardi; Des M; Resti Fevria; Yusni Atifah; Elsa Badriyya; Afifatul Achyar
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

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Sweet potato varieties (Ipomoea batatas) Pucuk Hitam Panyalaian and Madu Pucuk Hitam Panyalaian have the potential to be a superior commodity in West Sumatra, but the information of genetic diversity is very limited. Data on the diversity of sweet potato germplasm is beneficial in the selection of plants to obtain superior cultivars in plant breeding. Aim: The aim of the study was to find specific primers for the study of sweet potato genetic markers using Ipomoea batatas chloroplast genes matK. Methods: DNA extraction from sweet potato young leaves; designing forward and reverse primers Ipomoea batatas chloroplast genes matK; PCR; sequencing; bioinformatics analysis and species identification by comparing the NCBI database. Results: Plant DNA barcoding PCR using designed primers matK were successfully resulting single DNA band in different amplicon size in some samples. This indicating that the designed primers used were able to distinguish variation in one species.

Pedigree analysis of diabetes mellitus in Minangkabau ethnic S. Syamsurizal; Siti Halifah; Elsa Badriyya
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Diabetes mellitus has a tendency to be inherited. The inheritance pattern of diabetes mellitus in the Minangkabau ethnic group follows the mitochondrial and autosomal inheritance pattern. Patients with diabetes mellitus in the Minangkabau ethnicity have a equal distribution between men and women. Patients with diabetes mellitus in this ethnic group generally know that they suffer from diabetes mellitus in old age or after other complications arise due to diabetes mellitus.

Primers Designed For Amplifying TCF7L2 Gen S. Syamsurizal; Hengki Saputra; Dwi Hilda Putri; Elsa Badriyya
Biota Vol 6 No 2 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/10.19109/Biota.v6i2.5871

Diabetes Mellitus (DM) is a disease characterized by high glucose levels because of the lack of insulin hormone in the body. Prevalence of Type-2 Diabetes Mellitus is associated with the variant of the TCF7L2 gene polymorphism, or SNP. The purpose of this study was to construct a specific primer to amplify the TCF7L2 gene fragment and their capabilities to detect the targeted area. This research was a descriptive study that design the primers using Geneious software version 5.4.7. The study was conducted at the Laboratory of Genetics and Biotechnology FMIPA Universitas Negeri Padang. Based on this research, a 10qF forward primer (NG_012631.1) and 10qR reverse primers (NG_012631.1) has successfully designed. The primers can amplify the targeted area of the TCF7L2 gene with 1208 bp length. The amplified gene region has many variants that are strictly related to the appearance of Type 2 Diabetes Mellitus.

EDUKASI PENGGUNAAN OBAT BERBASIS KUNJUNGAN RUMAH UNTUK MENINGKATKAN PENGETAHUAN MASYARAKAT DI NAGARI SIKUCUR, KABUPATEN PADANG PARIAMAN Lailaturrahmi Lailaturrahmi; Elsa Badriyya; Annisa Fauzana; Dita Permatasari
BULETIN ILMIAH NAGARI MEMBANGUN Vol 7 No 1 (2024)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v7i1.450

The trend of independent drug use behavior (self-medication) among Indonesian people, especially in West Sumatra Province, continues to increase. Without knowledge about the correct use of drugs, people will be at risk to drug use problems. Therefore, education on drug use needs to be implemented to increase public knowledge regarding drug use. By increasing their knowledge, the public will have rational drug use behavior. The aim of this community outreach is to increase public knowledge regarding the use of over-the-counter drugs and limited over-the-counter drugs in treating health problems. The method of activity carried out is education through face-to-face home visits. This activity was carried out in Nagari Sikucur, V Koto Kampung Dalam District, Padang Pariaman Regency. There were 30 community members or family representatives who were the targets of this drug use education and home visit. The implementation of this activity involved five teams consisting of pharmacy lecturers with pharmacists’ qualification, Master of Pharmacy students who had registered as pharmacists, and Bachelor of Pharmacy students. The activity started with an introduction, continued with a pre-test, providing education, post-test, and handing over self-medication drug packages to the community. The results of this home visit-based education show that the community's knowledge score regarding drugs is higher than before the education. It can be concluded that the objectives of this activity were achieved well. To achieve better activity results, preliminary studies, collaboration with stakeholders, time allocation, and adequate technical preparation of activities are required.

Ketoprofen-Tromethamine: Binary Phase Diagram of Multicomponent Crystal, Dissolution Rate, and Analgesic Activity Evaluation Hasanah, Uswatul; Badriyya, Elsa; Safitri, Reza; Yuliza, Sukma; Ihsan, Ikhwanul; Saafrida; Rosaini, Henni; Jessica, Adhitya; Zaini, Erizal
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.726-734

Ketoprofen is a non-steroidal anti-inflammatory drug (NSAID) whose formulation options are limited due to its low dissolution rate in aqueous media. This research aimed to enhance the solubility of ketoprofen in distilled water and to compare the anti-inflammatory and analgesic effects of its resulting multicomponent crystal with tromethamine. The binary phase diagram of ketoprofen-tromethamine was created across molar ratios ranging from 1:9 to 9:1. The multicomponent crystal comprising ketoprofen and tromethamine in the selected ratio was synthesized using a solvent drop grinding method and subjected to further characterization for thermal properties, crystallinity, chemical groups, and morphology. The dissolution rate assessments were evaluated in CO2-free distilled water. Pharmacological analyses examined the anti-inflammatory and analgesic effects of the multicomponent crystal. The binary phase analysis identified the 5:5 (1:1) molar ratio as optimal in forming a multicomponent crystal. Thermograms and diffractograms revealed crystalline alterations attributed to a new crystalline phase. The new multicomponent crystal exhibited approximately 2.7 times higher dissolution rate after 30 minutes, outperforming pure ketoprofen. Pharmacological assessments demonstrated superior analgesic effects of the multicomponent crystal. In summary, the ketoprofen-tromethamine cocrystal in 1:1 molar ratio offers enhanced dissolution rate and provides better analgesic activity than ketoprofen alone.

Empowerment of UMKM Umah Labah An Nahl Farm and Education in improving Trigona honey bee cultivation Syamsurizal, S.; Fuadiyah, Sa'diatul; Kurniawati, Tri; Badriyya, Elsa
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/269

The kelulut / Trigona bee business development sector is still carried out as a small-scale business and is still far away when compared to other countries that have been able to produce large-scale honey production. Indonesia's honey production has only reached around 2,000 tons / year with a low level of honey consumption per capital, which is around 10 to 15 grams / person / year or only equivalent to one tablespoon per person per year. UMKM Umah Labah An Nahl Farm and Education located in Cimpua Pasa Durian village, Nagari Lareh Nan Panjang Sungai Sariak, Padang Pariaman Regency was founded by Febri Mulyadi, S.Pd. with two colleagues two years ago. In general, kelulut beekeepers in Cimpua village have not been organized in containers such as farmer groups or cooperatives so that they work individually so that their business progress has not been encouraging, including Umah Labah. This UMKM Umah Labah An Nahl Farm and Education is just developing and has various problems, including: 1. Marketing or marketing; 2. Educating consumers about the taste and quality of honey; 3. Working partners (retailers and so on) in adjusting prices; 4. Choosing good and attractive packaging and 5. Taking care of licensing correspondence. Thepurpose of the service is to develop superior products of Padang Pariaman Regency produced by Umah Labah An Nahl Farm and Education MSMEs to improve the quality and quantity of products / services to be highly competitive

EDUKASI PENGGUNAAN OBAT BERBASIS KUNJUNGAN RUMAH UNTUK MENINGKATKAN PENGETAHUAN MASYARAKAT DI NAGARI SIKUCUR, KABUPATEN PADANG PARIAMAN Lailaturrahmi Lailaturrahmi; Elsa Badriyya; Annisa Fauzana; Dita Permatasari
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 7 No. 1 (2024)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v7i1.450

The trend of independent drug use behavior (self-medication) among Indonesian people, especially in West Sumatra Province, continues to increase. Without knowledge about the correct use of drugs, people will be at risk to drug use problems. Therefore, education on drug use needs to be implemented to increase public knowledge regarding drug use. By increasing their knowledge, the public will have rational drug use behavior. The aim of this community outreach is to increase public knowledge regarding the use of over-the-counter drugs and limited over-the-counter drugs in treating health problems. The method of activity carried out is education through face-to-face home visits. This activity was carried out in Nagari Sikucur, V Koto Kampung Dalam District, Padang Pariaman Regency. There were 30 community members or family representatives who were the targets of this drug use education and home visit. The implementation of this activity involved five teams consisting of pharmacy lecturers with pharmacists’ qualification, Master of Pharmacy students who had registered as pharmacists, and Bachelor of Pharmacy students. The activity started with an introduction, continued with a pre-test, providing education, post-test, and handing over self-medication drug packages to the community. The results of this home visit-based education show that the community's knowledge score regarding drugs is higher than before the education. It can be concluded that the objectives of this activity were achieved well. To achieve better activity results, preliminary studies, collaboration with stakeholders, time allocation, and adequate technical preparation of activities are required.

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