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All Journal Biota EnviroScienteae Tropical Wetland Journal Journal of Applied Data Sciences Jurnal Sylva Lestari JURNAL HUTAN TROPIS Journal of the Community Development in Asia
Gusti Muhammad Hatta
Program Studi Budidaya Hutan, Fakultas Kehutanan, Universitas Lambung Mangkurat
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Other

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Journal : Biota

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Induction of in Vitro Germination of Tandui (Mangifera rufocostata Kostrem.): Effect of Antioxidants and 2,4-dichlorophenoxyacetic Acid Fitriani, Adistina; Arifin, Yudi Firmanul; Hatta, Gusti Muhammad; Wahdah, Raihani
Biota Vol 11 No 1 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v11i1.22190

Abstract

The bark of tandui (Mangifera rufocostata Kostrem.) is commonly used as a medicine for diabetes. Regeneration of this plant is difficult, and continuous harvesting of the bark leads to a decrease in the plant population. The purpose of this research is to apply tissue culture techniques for the propagation of tandui. Different antioxidants (KNO3, polyvinylpyrrolidone (PVP), Murashige and Skoog (MS) media + PVP, and MS + ascorbic acid) and varying concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.8, 1.0, 1.2, and 1.4 mg L–1) were tested for embryogenic tandui. The application of KNO3 and PVP suppressed the formation of browning in the media and explant of tandui. Soaking the explants in KNO3 and PVP reduce browning to 35% and 20%, respectively. The results also showed that increasing the concentration of 2,4-D enhanced the percentage of embryogenic tandui. Supplementing the media with 1.4 mg L–1 2,4-D resulted in 90% of embryogenic tandui. This study demonstrates that pre-soaking explants in antioxidants significantly reduces media browning, and supplementation of MS media with 2,4-D enhance embryogenic process. Thus, micropropagation of tandui could be achieved on a commercial basis.
Co-Authors Abdi Fithria Adistina Fitriani Ahmad Jauhari Asy'ari, Mufidah Fakhrur Razie Hadi, Wirawan Noor Hafizianor Hafizianor Hafizianor, Hafizianor Hanafi, Nanang Kissinger Kissinger Mochamad Arief Soendjoto Muhammad Helmi Nirwana, Hanifah Dwi Nisa, Fitriatun Noor, Ahmad Sugian Nugroho, Agung Raihani Wahdah Rodi Rodi, Rodi Rosadi, Hadi Saidy, Akhmad Rizalli Suyanto Suyanto Yudi Firmanul Arifin