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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Jurnal Ergonomi Indonesia (The Indonesian Journal of Ergonomic) Jurnal Pendidikan Biologi Undiksha Jurnal IKA Prosiding Seminar Nasional MIPA Jurnal Sains dan Teknologi Jurnal Pendidikan dan Pembelajaran IPA Indonesia Jurnal Pendidikan dan Pembelajaran Sains Indonesia (JPPSI) Jurnal Pendidikan dan Pembelajaran Sains Indonesia (JPPSI) Jurnal Puruhita
Ni Putu Ristiati
Universitas Pendidikan Ganesha
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Environmental Science Industrial & Manufacturing Engineering Public Health Social Sciences

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PENGARUH PEMBUATAN PARTICIPATORY VIDEO BERBASIS LANSKAP BUDAYA SUBAK MELALUI MODEL PEMBELAJARAN SALINGTEMAS TERHADAP HASIL BELAJAR SISWA SMA DITINJAU DARI KEMAUAN KERJA SAMA THALIA PRASETYA .; PROF.DR. NI PUTU RISTIATI, M.Pd. .; PROF. I WAYAN SUBAGIA, M.App.Sc.,Ph.D .
Jurnal Pendidikan dan Pembelajaran IPA Indonesia Vol 6, No 1 (2016)
Publisher : Jurnal Pendidikan dan Pembelajaran IPA Indonesia

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Abstract

Tujuan dari penelitian ini adalah untuk mendeskripsikan dan menjelaskan perbedaan hasil belajar antara siswa yang membuat participatory video (PV) berbasis lanskap budaya subak melalui model pembelajaran salingtemas (sains, lingkungan, teknologi dan masyarakat) dengan siswa yang tanpa membuat PV berbasis lanskap budaya subak melalui model pembelajaran salingtemas serta mendeskripsikan dan menjelaskan pengaruh interaksi antara model pembelajaran dan kemauan kerja sama terhadap hasil belajar siswa. Penelitian ini merupakan penelitian eksperimen dengan rancangan Non Equivalent Pretest-Posttest Control Group Design. Populasi penelitian merupakan seluruh siswa kelas X di SMA N 2 Mengwi tahun ajaran 2015/2016 dan dipilih empat kelas sebagai sampel. Data dianalisis dengan uji analisis varians (ANAVA) dan dilanjutkan uji Scheffe jika ditemukan pengaruh interaksi. Dari analisis data, menunjukkan adanya perbedaan hasil belajar antara siswa membuat PV berbasis lanskap budaya subak melalui model pembelajaran salingtemas dengan siswa yang tanpa membuat PV berbasis lanskap budaya subak melalui model pembelajaran salingtemas dan hasil uji juga menunjukkan adanya pengaruh pembuatan PV berbasis lanskap budaya subak melalui model pembelajaran salingtemas dan kemauan kerja sama terhadap hasil belajar siswa. Dari hasil tersebut dapat disimpulkan bahwa pembuatan PV berbasis lanskap budaya subak dapat meningkatkan hasil belajar serta membuat pembelajaran menjadi lebih bermakna.Kata Kunci : participatory video, subak, salingtemas, hasil belajar This study aimed at describing and explaining the difference of learning achievement between students that made participatory video (PV) based on subak landscape through SETS (sains, environment, technology and society) learning model and students that did not make any PV based on subak landscape through SETS learning model as well as describing and explaining the influence of interaction between the learning models and cooperation willingness towards students learning achievement. This study was an experiment research using Non Equivalent Pretest-Posttest Control Group Design. The population of the study was tenth grade students of SMA N 2 Mengwi in the academic year 2015/2016 and four class were selected as the sample. The data was analyzed by ANAVA test and continued by Scheffe test if the influence of interaction was found. From data analysis, indicated the difference of learning achievement between students that made PV based on subak landscape through SETS learning model and students that did not make any PV based on subak landscape through SETS learning model and the test result showed there was influence of PV making based on subak landscape through SETS learning model and teamwork willingness towards students learning achievement. From the result can be concluded that PV making based on subak landscape can improve learning achievement and make learning process become more meaningful.keyword : participatory video, subak, SETS, learning achievement
PHOSPHATASE ACTIVITY AND PHOSPHATE SOLUBILITY BY PHOSPHATE SOLUBILIZING RHIZOBACTERIA IN VOLCANIC SOILS OF PANCASARI, BALI Ketut Dharma Susila; I Made Sudana; Ni Putu Ristiati; I Made Adnyana
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Phosphatase in the soil was found as extracellular enzymes produced by soil micro-organisms both in acidic or alkaline conditions. Phosphatase is an enzyme complex that plays an important role in deciding soil-bound phosphate bond organic compounds to form orthophosphate which is available to the plant. Mineralization of organic forms into a inorganic-P determined by the ability of these bacteria to produce phosphatase. Therefore, observations of phosphatase activities are important to know how intensely P mineralization process takes place in the soil. Phosphate solubilizing rhizobacteria (PSR) has been widely known to affect mobilization of insoluble inorganic phosphates become available to plants. Although phosphate solubilizing rhizobacteria is widely available in the soil, their activity is usually not strong enough to compete with other bacteria commonly located in the root zone (rhizosphere). That is why, screening of phosphatase enzyme activity and various phosphate solubilizing ability to obtain better strains of bacteria are still needed. This study was conducted to determine the ability of phosphate solubilizing rhizobacteria, its potential as a biofertilizer inoculant and characterize its capacity to dissolve P-insoluble inorganic and produce phosphatase enzymes in the growing medium. Potential phosphate solubilizing rhizobacteria was tested for their ability in vitro using Pikovskaya media containing insoluble forms of inorganic phosphate as a source P. The results of this study indicate that there are some isolates phosphate solubilizing rhizobacteria that are capable of dissolving the insoluble inorganic-P with different abilities. The highest potential successively displayed by isolates TbPP-4.1; BdPP-2.1; and SBPP-1.3 that significantly has the same ability to soluble inorganic phosphate in the soil. The isolates BdPP-2.1 and TbPP-4.1 are significantly higher than the other isolates for producing phosphatase. Both isolates TbPP-4.1 and BdPP-2.1 have the same pontenty as a biofertilizer based on its capacity to mineralize forms of soil organic phosphorus by phosphatase activity.
Potensi Alga Merah dan Alga Hijau untuk Menghambat Pertumbuhan Bakteri Eschericia coli KETUT SRIE MARHAENI JULYASIH; NI PUTU RISTIATI; IDA BAGUS PUTU ARNYANA
Agrotrop : Journal on Agriculture Science Vol 10 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.65 KB) | DOI: 10.24843/AJoAS.2020.v10.i01.p02

Abstract

Potential of Red Algae and Green Algae to Inhibit Escherichia coli Bacteria Growth. The use of synthetic chemicals as a control for the growth of E. coli bacteria that causes of diarrhea children can cause adverse effects on health, so it needs natural control ingredients that do not cause impacts on human health. One natural controller to inhibit bacterial growth is by utilizing natural sources, including seaweed. The purpose of this study was to examine the potential of seaweed as an anti-bacterial agent of E. coli. The research used seaweed extract of Eucheuma spinosum, Euchuema cottonii, Gracilaria spp., and Caulerpa spp. The test parameters observed were the inhibition zone (mm) of each seaweed extract treatment using a ruler and measured the inhibition zone distance. The results showed that E.cottonii seaweed extract had the highest inhibitory ability with an inhibition zone diameter of 11.1 mm, including the moderately sensitive category, then Caulerpa spp. with a inhibition zone diameter of 10.0 mm, Gracilaria spp has the ability to inhibit E.coli bacteria with a inhibition zone diameter of 6.9 mm, including the insensitive category, and the lowest in seaweed E. spinosum with a inhibition zone diameter of 4.2 mm. including the insensitive category.
PENGARUH EKSTRAK DAUN SIRIH (Piper betle Linn.) DAN EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb.) DENGAN KONSENTRASI YANG BERBEDA TERHADAP PERTUMBUHAN Streptococcus mutans Ni Kadek Pinawati .; Prof. Dr. Ni Putu Ristiati, M.Pd. .; Drs. Sanusi Mulyadiharja,M.Pd. .
Jurnal Pendidikan Biologi undiksha Vol. 1 No. 1 (2014)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v1i1.3260

Abstract

Penelitian ini bertujuan untuk mengetahui (1) perbedaan diameter zona hambatan pertumbuhan Streptococcus mutans akibat pemberian ekstrak daun sirih dengan konsentrasi 30%, 45% dan 60%; (2) perbedaan diameter zona hambatan pertumbuhan Streptococcus mutans akibat pemberian ekstrak rimpang temulawak dengan konsentrasi 30%, 45% dan 60%); (3) adanya interaksi antara masing-masing jenis ekstrak dengan konsentrasi yang berbeda terhadap diameter zona hambatan pertumbuhan Streptococcus mutans. Populasi dalam penelitian ini adalah biakan murni Streptococcus mutans dengan sampel Streptococcus mutans yang diencerkan dengan aquades steril sebanyak 10 ml. Metode pengumpulan data yang digunakan dalam penelitian ini adalah metode observasi. Teknik analisis data dengan Anova dua arah. Hasil penelitian ini adalah (1) terdapat perbedaan diameter zona hambatan pertumbuhan Streptococcus mutans akibat pemberian ekstrak daun sirih dengan konsentrasi 30%, 45% dan 60%. (2) terdapat perbedaan diameter zona hambatan pertumbuhan Streptococcus mutans akibat pemberian ekstrak rimpang temulawak dengan konsentrasi 30%, 45% dan 60%. (3) Tidak ada interaksi antara masing-masing jenis ekstrak dengan konsentrasi yang berbeda terhadap diameter zona hambatan pertumbuhan Streptococcus mutans. Kata Kunci : ekstrak daun sirih, ekstrak rimpang temulawak, Streptococcus mutans This study aims to (1) determine differences in growth inhibition zone diameter of Streptococcus mutans due to betel leaf extract with a concentration of 30%, 45% and 60%. (2) determine differences in growth inhibition zone diameter of Streptococcus mutans due to the ginger rhizome extract with a concentration of 30%, 45% and 60%). (3) determine the interaction between each type of extract with different concentrations of the growth inhibition zone diameter of Streptococcus mutans. The population in this study was a pure culture of Streptococcus mutans and Streptococcus mutans samples were diluted with 10 mL of sterile distilled water. Data collection methods used in this study is the observation method. Data analysis techniques with two-way ANOVA. The results are (1) there is a difference in diameter of inhibition zone due to the growth of Streptococcus mutans betel leaf extract with a concentration of 30%, 45% and 60%; (2) there is a difference in diameter of the zones of growth inhibition of Streptococcus mutans due to the ginger rhizome extract with a concentration of 30%, 45% and 60%; (3) There is no interaction between each type of extract with different concentrations of the growth inhibition zone diameter of Streptococcus mutans.keyword : betel leaf extract, ginger rhizome extract, Streptococcus mutans
ANALISIS PERBEDAAN MASSA PADI VARIETAS IR64 SEBAGAI MEDIA TUMBUH TERHADAP KADAR PIGMEN MERAH PADA ANGKAK YANG DIHASILKAN OLEH Monascus Purpureus Gst. Ayu Md. Juniasmita Parsandi .; Prof. Dr. Ni Putu Ristiati, M.Pd. .; Drs. Sanusi Mulyadiharja,M.Pd. .
Jurnal Pendidikan Biologi undiksha Vol. 1 No. 1 (2014)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v1i1.3554

Abstract

Tujuan penelitian ini adalah (1) mengetahui perbedaan massa padi varietas IR64 sebagai media tumbuh terhadap kadar pigmen merah angkak yang dihasilkan oleh Monascus purpureus, (2) mengetahui massa padi varietas IR64 yang digunakan dalam fermentasi angkak ditinjau dari kadar pigmen tertinggi, (3) mengetahui kuantitas produk angkak ditinjau dari pH, dan bobot angkak. Jenis penelitian yang dilakukan merupakan penelitian eksperimen dan rancangan penelitian adalah Rancangan Acak Lengkap (RAL). Variabel bebas dalam penelitian ini adalah massa padi varietas IR64 yakni 30 gram, 40 gram, 50 gram dan 60 gram sebagai media tumbuh Monascus purpureus, variabel terikat adalah kadar pigmen merah pada angkak diukur berdasarkan Optical Density (OD) dan variabel kontrol adalah kelembaban, suhu, dan intensitas cahaya. Analisis data dilakukan dengan menggunakan statistik parametrik, uji hipotesis dengan Anova One Way, kemudian dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil dari penelitian ini adalah (1) terdapat perbedaan kadar pigmen merah pada angkak oleh Monascus purpureus berdasarkan perbedaan massa padi varietas IR64 dilihat dari nilai F hitung > F tabel yakni 92,824 > 1,714, (2) Massa padi varietas IR64 yang dapat digunakan dalam fermentasi angkak ditinjau dari kadar pigmen angkak tertinggi melalui uji BNT dengan taraf signifikansi sebesar 0,000 < 0,05, sehingga rata rata perlakuan memiliki perbedaan signifikansi yakni massa beras 30 gram dengan kadar pigmen merah tertinggi sebesar 1,9 g/100 ml, (3) pH optimal dalam menghasilkan kadar pigmen adalah 6,03 dari kriteria pH 6 terjadi penurunan bobot angkak setelah fermentasi dan pengovenan. Kata Kunci : Monascus purpureus, Angkak, Padi Varietas IR64. The purpose of this study is (1) determine the mass difference IR64 rice as a growing medium on levels of red yeast rice red pigment produced by Monascus purpureus, (2) determine the mass of IR64 rice varieties used in the fermentation of red yeast rice in terms of the highest pigment levels, (3) determine quantity of red yeast rice products in terms of pH, and the weight of red yeast rice. Type of research is experimental research and the study design was completely randomized design (CRD). The independent variable in this study is that the mass of IR64 rice 30 grams, 40 grams, 50 grams and 60 grams of Monascus purpureus as a growing medium, the dependent variable is the red pigment in red yeast rice levels were measured by Optical Density (OD) and the control variable is humidity, temperature, and light intensity. Data analysis was performed using parametric statistics, hypothesis testing with ANOVA One Way, followed by the Least Significant Difference test (LSD). The results of this study were (1) there are differences in the levels of red pigment in red yeast rice by Monascus purpureus by the mass difference of rice IR64 seen from F count> F table is 92,824 > 1,714, (2) mass IR64 rice varieties that can be used in the fermentation of red yeast rice in terms of the highest levels of pigment red yeast rice through LSD test with a significance level of 0,000 < 0,05, so the average treatment has significant differences that mass 30 grams rice with red pigment highest levels of 1,90 g / 100 ml, (3) the optimal pH produce pigment concentration is 6.03 of criteria weights decreased pH 6 after fermentation of red yeast rice and oven. keyword : Monascus purpureus, Angkak, Rice Variety IR64
EFEKTIVITAS EKSTRAK KASAR DAUN KELOR (Moringa oleifera) DENGAN KONSENTRASI BERBEDA TERHADAP DAYA HAMBAT KOLONI BAKTERI DARI RONGGA MULUT MANUSIA I Wayan Suparyanta .; Prof. Dr. Ni Putu Ristiati, M.Pd. .; Drs. Sanusi Mulyadiharja,M.Pd. .
Jurnal Pendidikan Biologi undiksha Vol. 2 No. 1 (2015)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v2i1.5540

Abstract

Kesehatan rongga mulut tidak dapat dipisahkan dari kesehatan tubuh secara menyeluruh. Kebersihannya yang kurang baik dapat menimbulkan gangguan yang umumnya disebabkan oleh bakteri. Kebersihan rongga mulut dapat dijaga dengan menggosok gigi, penggunaan obat kumur, serta bahan alami yang salah satunya berasal dari tanaman kelor (Moringa oleifera). Penelitian ini bertujuan (1) mengetahui ada tidaknya perbedaan daya hambat koloni bakteri dari rongga mulut manusia setelah pemberian ekstrak kasar daun kelor pada konsentrasi berbeda, (2) mengetahui konsentrasi ekstrak kasar daun kelor yang paling efektif dari tiga variasi konsentrasi 10%, 20%, dan 30% terhadap daya hambat koloni bakteri dari rongga mulut manusia, (3) mengidentifikasi karakteristik genus bakteri yang diisolasi dari rongga mulut manusia. Jenis penelitian ini adalah penelitian eksperimen sungguhan dengan Rancangan Acak Lengkap (RAL) posttest-only control group design. Populasi dalam penelitian ini adalah polikultur bakteri dari rongga mulut manusia. Bakteri ditumbuhkan pada media Nutrient Agar digunakan sebagai sampel diperoleh dengan cara random sederhana. Data dianalisis menggunakan uji statistik ANAVA satu arah. Hasil penelitian menunjukan (1) ada perbedaan daya hambat koloni bakteri dari rongga mulut manusia setelah pemberian ekstrak kasar daun kelor pada konsentrasi berbeda berdasarkan hasil uji statistik ANAVA satu arah yakni angka signifikansi < 0,05 dengan taraf signifikansi 5%, serta nilai F hitung 288,751 > F tabel 3,47 yang berarti H0 ditolak dan H1 diterima. (2) konsentrasi ekstrak kasar daun kelor yang paling efektif adalah konsentrasi 30% dengan rerata koloni bakteri 16,5. (3) genus bakteri yang ditemukan pada rongga mulut manusia adalah Staphylococcus, Bacillus, Neisseria, dan Lactobacillus.Kata Kunci : Efektivitas, Ekstrak kasar daun kelor (Moringa oleifera), Rongga mulut manusia. Healty of oral cavity cannot separated from overall body health. Poor hygine of oral cavity can cause disease generaly caused by bacteria. Oral cavity hygiene could care by brush teeth, mouthwash, and also natural material which one from moringa plant (Moringa oleifera). The purpose of this research was (1) to know absence or present the differences against inhibition colonies of bacteria from human oral cavity after treatment by moringa leaf crude extract with difference concentration, (2) to know moringa leaf crude extract that most effective from three variance concentration 10%, 20%, and 30% to inhibition colonies of bacteria from human oral cavity, (3) to identified characteristic bacterial genus that isolated from human oral cavity. This research was true experimental research with Complete Random Design (CRD) posttest-only control group design. Population of this research was bacterial polyculture from human oral cavity. Bactery was grown on Nutrient Agar as sample on this research collected by simple random sampling. Data analysis tecniques was one way ANOVA. The result of the research there are (1) present the differences against inhibition colonies bacteria from human oral cavity after treatment by moringa leaf crude extract with difference concentration based from one way ANOVA statistical test result was
EFEKTIVITAS EKSTRAK KASAR DAUN KELOR (Moringa oleifera) DENGAN KONSENTRASI BERBEDA TERHADAP DAYA HAMBAT KOLONI BAKTERI DARI RONGGA MULUT MANUSIA I Wayan Suparyanta .; Prof. Dr. Ni Putu Ristiati, M.Pd. .; Drs. Sanusi Mulyadiharja,M.Pd. .
Jurnal Pendidikan Biologi undiksha Vol. 2 No. 1 (2015)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v2i1.5541

Abstract

Kesehatan rongga mulut tidak dapat dipisahkan dari kesehatan tubuh secara menyeluruh. Kebersihannya yang kurang baik dapat menimbulkan gangguan yang umumnya disebabkan oleh bakteri. Kebersihan rongga mulut dapat dijaga dengan menggosok gigi, penggunaan obat kumur, serta bahan alami yang salah satunya berasal dari tanaman kelor (Moringa oleifera). Penelitian ini bertujuan (1) mengetahui ada tidaknya perbedaan daya hambat koloni bakteri dari rongga mulut manusia setelah pemberian ekstrak kasar daun kelor pada konsentrasi berbeda, (2) mengetahui konsentrasi ekstrak kasar daun kelor yang paling efektif dari tiga variasi konsentrasi 10%, 20%, dan 30% terhadap daya hambat koloni bakteri dari rongga mulut manusia, (3) mengidentifikasi karakteristik genus bakteri yang diisolasi dari rongga mulut manusia. Jenis penelitian ini adalah penelitian eksperimen sungguhan dengan Rancangan Acak Lengkap (RAL) posttest-only control group design. Populasi dalam penelitian ini adalah polikultur bakteri dari rongga mulut manusia. Bakteri ditumbuhkan pada media Nutrient Agar digunakan sebagai sampel diperoleh dengan cara random sederhana. Data dianalisis menggunakan uji statistik ANAVA satu arah. Hasil penelitian menunjukan (1) ada perbedaan daya hambat koloni bakteri dari rongga mulut manusia setelah pemberian ekstrak kasar daun kelor pada konsentrasi berbeda berdasarkan hasil uji statistik ANAVA satu arah yakni angka signifikansi < 0,05 dengan taraf signifikansi 5%, serta nilai F hitung 288,751 > F tabel 3,47 yang berarti H0 ditolak dan H1 diterima. (2) konsentrasi ekstrak kasar daun kelor yang paling efektif adalah konsentrasi 30% dengan rerata koloni bakteri 16,5. (3) genus bakteri yang ditemukan pada rongga mulut manusia adalah Staphylococcus, Bacillus, Neisseria, dan Lactobacillus.Kata Kunci : Efektivitas, Ekstrak kasar daun kelor (Moringa oleifera), Rongga mulut manusia. Healty of oral cavity cannot separated from overall body health. Poor hygine of oral cavity can cause disease generaly caused by bacteria. Oral cavity hygiene could care by brush teeth, mouthwash, and also natural material which one from moringa plant (Moringa oleifera). The purpose of this research was (1) to know absence or present the differences against inhibition colonies of bacteria from human oral cavity after treatment by moringa leaf crude extract with difference concentration, (2) to know moringa leaf crude extract that most effective from three variance concentration 10%, 20%, and 30% to inhibition colonies of bacteria from human oral cavity, (3) to identified characteristic bacterial genus that isolated from human oral cavity. This research was true experimental research with Complete Random Design (CRD) posttest-only control group design. Population of this research was bacterial polyculture from human oral cavity. Bactery was grown on Nutrient Agar as sample on this research collected by simple random sampling. Data analysis tecniques was one way ANOVA. The result of the research there are (1) present the differences against inhibition colonies bacteria from human oral cavity after treatment by moringa leaf crude extract with difference concentration based from one way ANOVA statistical test result was
UJI VIABILITAS DAN KEMAMPUAN DEGRADASI MINYAK SOLAR OLEH KONSORSIUM BAKTERI HASIL PRESERVASI TERHADAP KADAR ASAM n-OKTANOAT Ni Luh Ayu Sri Purwaningsih .; Prof. Dr. Ni Putu Ristiati, M.Pd. .; Drs. Sanusi Mulyadiharja,M.Pd. .
Jurnal Pendidikan Biologi undiksha Vol. 3 No. 1 (2016)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v3i1.7364

Abstract

Lingkungan perairan laut seringkali digunakan sebagai jalur transportasi air, dengan adanya jalur transportasi air ini tidak menutup kemungkinan juga akan menghasilkan produk samping atau limbah yang dapat mencemari lingkungan. Secara biologis, biodegradasi oleh mikroba merupakan salah satu cara yang tepat, efektif dan hampir tidak ada pengaruh samping pada lingkungan. Dalam penelitian ini, dilakukan dua uji yaitu uji viabilitas bakteri dan uji kemampuan degradasi minyak solar oleh bakteri konsorsium hasil preservasi. Hasil yang diperoleh yaitu ditemukan 4 genus bakteri yang mampu bertahan hidup diantaranya isolat Pseudomonas, Neisseria, Acetobacter dan Halomonas. Pada uji kemampuan degradasi terdapat adanya pengaruh perbedaan kadar Asam n-Oktanoat yang dihasilkan dengan konsentrasi yang berbeda, dilihat dari nilai signifikansi diperoleh < 0,05 yaitu 0,000 dan Fhitung = 14,836 > Ftabel = 5,14, maka terdapat perbedaan konsentrasi konsorsium bakteri dengan perlakuan yang berbeda-beda yaitu 10ml, 20ml dan 30ml pada Media Bushnell Haas Mineral Salts yang ditambahkan susu skim terhadap kadar Asam n-Oktanoat yang dihasilkan oleh bakteri konsorsium hasil preservasi dalam mendegradasi minyak solar. Kata Kunci : Asam n-Oktanoat, Degradasi Minyak Solar, Konsorsium Bakteri Marine environment is often used as a transportation of water, in the presence of water transport lines did not rule out also will generate byproducts or waste that can pollute the environment. Biologically, biodegradation by microbes is one way that is appropriate, effective and virtually no side effects on the environment. In this study, conducted with two trials that test the viability of the bacteria and test capabilities diesel oil degradation by bacteria consortium preservation results.The results obtained are found four genus of bacteria are able to survive among isolates of Pseudomonas, Neisseria, Acetobacter and Halomonas. In the test the ability of degradation there is the effect of different levels of n-octanoic acid is produced with different concentrations, judging from the significant value gained < 0.05 is 0.000 and of F = 14.836 > Ftable = 5.14, then there are differences in the concentration of bacterial consortium with different treatment is 10ml, 20ml and 30ml in Bushnell Haas Media Mineral Salts added skim milk to high levels of n-octanoic acid produced by bacteria consortium preservation results in degrading of diesel oil. keyword : n-octanoic acid, Oil Degradation Solar, Bacterial Consortium
ANALISIS BAKTERI PADA RHIZOSFER HUTAN DASONG DAN PERKEBUNAN STROBERI (Fragaria ananassa) DI DESA PANCASARI, KECAMATAN SUKASADA, KABUPATEN BULELENG, BALI Ni Komang Deny Julyeda .; Prof. Dr. Ni Putu Ristiati, M.Pd. .; Drs. Sanusi Mulyadiharja,M.Pd. .
Jurnal Pendidikan Biologi undiksha Vol. 3 No. 1 (2016)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v3i1.7373

Abstract

Hutan Dasong di Desa Pancasari, memiliki kondisi tanah yang masih tergolong alami. Tidak jauh dari kawasan Hutan Dasong, terdapat perkebunan stroberi yang diberikan pestisida kimia secara berkelanjutan. Tujuan penelitian ini adalah (1) mengetahui jumlah koloni, (2) menganalisis karakteristik makroskopis koloni, serta (3) menganalisis Genus bakteri pada rhizosfer Hutan Dasong dan perkebunan stroberi di Desa Pancasari, Bali. Jenis penelitian ini adalah penelitian deskriptif. Isolasi bakteri menggunakan media Nutrient Agar dengan metode agar tuang, sedangkan penghitungan jumlah koloni bakteri dengan metode cawan hitung. Data dianalisis menggunakan uji beda. Hasil penelitian ini menunjukkan (1) adanya perbedaan jumlah koloni bakteri (p=0,037), (2) karakteristik makroskopis koloni bakteri di Hutan Dasong adalah bentuk (sirkuler, tak beraturan, rizoid), margin (mulus, filamentus, lobatus), pigmentasi (putih keabuan, putih pekat, putih, putih pudar, bening, kuning, kuning muda, jingga), dan elevasi (datar, naik, cembung), sedangkan karakteristik makroskopis koloni bakteri di perkebunan stroberi adalah bentuk (sirkuler, tak beraturan), margin (mulus, filamentus, lobatus, bergelombang, bergerigi), pigmentasi (putih pekat, putih, putih pudar, bening, jingga, kuning, putih kehijauan, putih kekuningan), dan elevasi (datar, naik, umbonatus), (3) terdapat 10 Genus bakteri yang terdapat pada rhizosfer Hutan Dasong yang diindikasikan sebagai Genus Ensifer, Rhizobacter, Agrobacterium, Acetobacterium, Arthrobacter, Bacillus, Microbacterium, Azomonas, Myxococcus, dan Micrococcus. Isolat P, Y, dan A1 diindikasikan sebagai Microbacterium lacticum, Microbacterium imperial, dan Micrococcus nishinomiyaensis. Terdapat 6 Genus pada rhizosfer perkebunan stroberi yang diindikasikan sebagai Genus Paracoccus, Arthrobacter, Pseudomonas, Micrococcus, Azotobacter, dan Flavobacterium. Genus Arthrobacter dan Micrococcus terdapat di kedua tempat karena tanah perkebunan stroberi mengambil tanah humus dari Hutan Dasong. Kata Kunci : Analisis Bakteri, Hutan Dasong, Perkebunan Stroberi, Rhizosfer Dasong Forest located in Pancasari Village, had a quite natural soil conditions and diversity of plant species. Near from Dasong Forest area, there was a strawberry farm which supplied by chemical pesticides on an ongoing basis. The purpose of this study was (1) to determine the number of colonies; (2) analyze the macroscopic characteristics of the colonies; and (3) the Genus of bacteria in the rhizosphere of Dasong Forest and strawberry farm in Pancasari Village, Bali. This research was a descriptive. Isolation of the bacteria were performed by Nutrient Agar using pour plate method, while to count the number of bacterial colonies using total plate count method. Data were analyzed by a different test (t-test). The results of this study showed (1) the differences number of bacterial colonies (p=0,037); (2) the macroscopic characteristics of bacteria colonies in Dasong Forest were the shapes (circular, irregular, rhizoid), margins (smooth, filamentous, lobatus), pigmentation (grayish-white, solid white, white, off-white, translucent, yellow, light yellow, orange), and the elevation (flat, rising, convex). Macroscopic characteristics of the bacteria colonies in strawberry farm were the shape (circular, irregular), margins (smooth, filamentous, lobatus, wavy, serrated), pigmentations (solid white, white, off-white, translucent, orange, yellow, greenish white, yellowish white), and elevation (flat, rising, umbonatus); (3) there are 10 genera of bacteria that found in the rhizosphere of Dasong Forest, its can be indicated as Genus of Ensifer, Rhizobacter, Agrobacterium, Acetobacterium, Arthrobacter, Bacillus, Microbacterium, Azomonas, Myxococcus, and Micrococcus. Isolate of P, Y, and A1 can be indicated as Genus of Microbacterium lacticum, Microbacterium imperial, and Micrococcus nishinomiyaensis. While there are 6 genera in the rhizosphere of strawberry farm, that can be indicated as Genus of Paracoccus, Arthrobacter, Pseudomonas, Micrococcus, Azotobacter, and Flavobacterium. Genus Arthrobacter and Micrococcus found in Dasong Forest and strawberry farm, because the soil of strawberry farm takes from soil of Dasong Forest. keyword : Analysis of Bacteria, Dasong Forest, Strawberry Farm, Rhizosphere
UJI KEMAMPUAN DEGRADASI MINYAK SOLAR OLEH KONSORSIUM BAKTERI HASIL PRESERVASI DENGAN KOMBINASI METODE LIOFILISASI DAN METODE GLISEROL I Made Gita Pramana Putra .; Prof. Dr. Ni Putu Ristiati, M.Pd. .; Drs. Sanusi Mulyadiharja,M.Pd. .
Jurnal Pendidikan Biologi undiksha Vol. 3 No. 1 (2016)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v3i1.7433

Abstract

Tujuan utama penelitian ini adalah untuk mengetahui perbedaan kadar Asam n-Oktanoat yang dihasilkan oleh konsorsium bakteri pendegradasi minyak solar hasil preservasi. Penelitian eksperimental ini menggunakan rancangan the randomized posttest only control group design dengan 8 kali pengulangan pada kelompok perlakuan volume 10 ml, 20 ml, dan 30 ml konsorsium bakteri pendegradasi minyak solar. Populasi penelitian ini adalah konsorsium bakteri pendegradasi minyak solar hasil preservasi menggunakan kombinasi metode liofilisasi dan metode gliserol. Sampel penelitian adalah cuplikan 5 ml media degradasi minyak solar dari masing-masing unit percobaan. Analisis data menggunakan Uji Anava satu arah dengan taraf signifikansi 5%. Hasil analisis data diperoleh Fhitung (32,615) < Ftabel (3,466) dan nilai signikansi 0,000 < 0,05 . Hal ini menunjukkan bahwa ada perbedaan kadar Asam n-Oktanoat yang dihasilkan oleh konsorsium bakteri pendegradasi minyak solar hasil preservasi. Volume 30 ml konsorsium bakteri pendegradasi minyak solar hasil preservasi adalah volume optimum dalam menghasilkan Asam n-Oktanoat. Hasil identifikasi isolat bakteri, ditemukan empat genus bakteri, yaitu: Neisseria (isolat A dan D), Pseudomonas (isolat B dan E), Acinetobacter (isolat C), dan Halomonas (isolat F).Kata Kunci : konsorsium, bakteri pendegradasi solar, Asam n-Oktanoat The main purpose of this study was to know the differences of n-octanoic acid level produced by an-after period of preservation bacteria consortium to degrade petroleum diesel. This experimental research design was the randomized posttest-only control group design with eight replication in three treatment groups from consortium of diesel oil degrading bacteria volume. The population was an-after period preservation diesel oil degrading bacteria consortium using combination of lyophilization method and glycerol method. Sample is a snippet of 5 ml of fuel oil degradation medium from each experiment unit. Data analysis using One Way Anova test with significance level of 5%. Based on the data analysis obtained Fhitung (32.615) < F tabel (3.466) and significance 0,000 < 0,005 shows that there are significant differences in levels of n-octanoic acid that produced by an-after period of diesel oil degrading bacteria consortium. 30 ml of an-after period preservation of diesel oil degrading bacteria consortium is the optimum volume in producing n-octanoic acid. The observations of secondary data found that there are four genus of six bacterial isolates, such as: Neisseria (A and D isolates), Pseudomonas (B and E isolates), Acinetobacter (C isolate), and Halomonas (F isolates).keyword : consortium, diesel degrading bacteria, n-octanoic acid
Co-Authors ., Agus Putu Adi Wyadnya Yoga ., ANA MAULANA ., Dewa Ayu Putu Inten Utari Dewi ., Erfiana Febry Puspitasari ., I GEDE DAKTARIANA U ., I Made Dicky Satya Narayana ., I Made Gita Pramana Putra ., I Putu Andy Putra ., IRQAMI RACHMA DWI D ., Komang Sani Willyarsa ., Ngakan Putu Ari Krisna Pratama ., Ni Komang Deny Julyeda ., Ni Luh Ayu Sri Purwaningsih ., Ni Putu Ayu Meita Kurniawati ., Ni Putu Hendrayani ., Ni Putu Oka Milarika ., Putu Ayu Agustina Saraswadewi ., Putu Cindy Arista ., Putu Paramesti Nopitayani ., Stanislaus Putu Mikael Mba Balu Agus Putu Adi Wyadnya Yoga . ANA MAULANA . Anantawikrama Tungga Atmadja Anggraini, Ni Wayan Yuliana AYU SRI WIDYANTINI . Dewa Ayu Putu Inten Utari Dewi . Dewa Ngurah Suprapta DIAN DWIPA JAYANTHI . DR. I GST.AGUNG NYM.SETIAWAN, M.Si. . Erfiana Febry Puspitasari . Gst. Ayu Md. Juniasmita Parsandi . I GEDE DAKTARIANA U . I Gede Sudirgayasa I Gusti Agung Nyoman Setiawan I M G. P. Putra I MADE ADNYANA I Made Dicky Satya Narayana . I Made Gita Pramana Putra . I Made Sudana I Made Sutajaya I Putu Andy Putra . I Wayan Suastra I Wayan Sukra Warpala I Wayan Suparyanta . I Wayan Suparyanta ., I Wayan Suparyanta Ida Ayu Putu Suryanti Ida Bagus Putu Arnyana ILMA WIRYANTI . IRQAMI RACHMA DWI D . Ketut Dharma Susila Ketut Srie Marhaeni Julyasih Ketut Suata Komang Sani Willyarsa . MADE AYU SRI ARIANI . MADE EKA ADNYANA . Ngakan Putu Ari Krisna Pratama . Ni Kadek Pinawati . Ni Komang Deny Julyeda . Ni Luh Ayu Sri Purwaningsih . Ni Luh Pande Latria Devi NI LUH PUTU MANIK WIDIYANTI Ni Made Nita Setiari Ni Nyoman Parwati Ni Putu Ayu Meita Kurniawati . Ni Putu Hendrayani . Ni Putu Oka Milarika . Ni Putu Sri Ratna Dewi NI WAYAN EMI SULANDARI . Ni Wayan Gunia Prastuti NI WAYAN MASIH . Ni Wayan Yuliana Anggraini Pradipta Utama, Putu Anggan PROF. I WAYAN SUBAGIA, M.App.Sc.,Ph.D . Putra, I M G. P. Putu Anggan Pradipta Utama Putu Ayu Agustina Saraswadewi . Putu Cindy Arista . PUTU EKA SASTRIKA AYU . Putu Paramesti Nopitayani . Sanusi Mulyadiharja Setiari, Ni Made Nita Stanislaus Putu Mikael Mba Balu . THALIA PRASETYA .