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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Current Biochemistry QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Entomologi Indonesia Indonesian Journal of Applied Research (IJAR) Journal of Fisheries & Marine Halal Studies and Society Indonesian Journal of Jamu
Purwanto, Ukhradiya Magharaniq Safira
Department Of Biochemistry, IPB University
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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Journal : Current Biochemistry

 1 
Isolasi Bakteri Endofit dari Tanaman Sirih Hijau (Piper betle L.) dan Potensinya sebagai Penghasil Senyawa Antibakteri Ukhradiya Magharaniq Safira; Fachriyan Hasmi Pasaribu; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing disruption or damage to the host. Some studies suggest that certain endophytic bacteria can produce chemical compounds that have an effect on health, especially endophytic bacteria isolated from medicinal plants. Green betel (Piper betle L.) is a medicinal plant that has been used for years and has many benefits. The purposes of this study are to isolating and screening of endophytic bacteria from green betel against four pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Bacillus cereus and Salmonella enteritidis). The number of endophytic bacteria that has been isolated are 14 isolates. Based on the screening results, three isolates of endophytic bacteria have potential activity (characterized by the formation of inhibition zone) against S. aureus. The inhibition zone may indicate that those isolates produce compounds that have antibacterial effects. Those isolates are AS1, BS1 and BS2. The biggest inhibition zone showed by BS1, so it can be concluded that BS1 is the most potential isolate as a novel source of antibacterial compound.
Perbedaan Bagian-bagian Tanaman Krokot (Portulaca grandiflora Hook.) terhadap Kandungan Total Fenolik dan Flavonoid serta Aktivitas Antioksidan Husnawati Husnawati; Ukhradiya Magharaniq Safira Purwanto; Aulia Ayu Rispriandari
Current Biochemistry Vol. 7 No. 1 (2020)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.7.1.2

Abstract

Antioksidan diperlukan untuk menangkal radikal bebas dalam tubuh serta stress oksidatif yang mendorong proses penuaan dan berbagai penyakit degeneratif. Portulaca grandiflora Hook. diketahui memiliki potensi sebagai antioksidan. Perbedaan bagian-bagian tanaman dapat menyebabkan perbedaan kandungan fitokimia, yang kemudian dapat mempengaruhi aktivitas antioksidannya. Penelitian ini bertujuan mengukur kandungan total fenolik dan flavonoid pada organ daun, batang tua, batang muda, dan bunga dari tanaman krokot (Portulaca grandiflora Hook.) yang diekstrak menggunakan etanol 96% serta menentukan aktivitas antioksidan dari ekstrak tersebut dengan metode DPPH. Hasil penelitian menunjukkan bahwa setiap organ tanaman krokot mengandung konsentrasi senyawa fenolik dan flavonoid yang berbeda. Kandungan total fenolik dan flavonoid paling tinggi terdapat pada bagian daun (113.26 ± 3.85 mg GAE/g dan 97.99 ± 1.28 mg QE/g), tetapi aktivias antioksidan tertinggi terdapat pada bagian batang tua dengan nilai IC50 122.15 ± 1.30 ppm (tergolong antioksidan sedang). Kata kunci: Antioksidan, Fenolik, Flavonoid, Portulaca grandiflora Hook.
Analysis of Metabolite Compound Profiles of Miana Leaves Endophytic Bacteria (Coleus scutellariodes) using GC-MS Dwi Endah Kusumawati; Ukhradiya Magharaniq Safira Purwanto; Maria Bintang; Fachriyan Hasmi Pasaribu
Current Biochemistry Vol. 8 No. 2 (2021)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.8.2.2

Abstract

Exploration of bioactive compounds from endophytic bacteria as novel source of antibacterial need to be explored, as an alternative to substitute synthetic antibiotics. Based on previous studies, DM6 was a potential isolate that showed antibacterial activity. The isolate has been identified as Brevibacillus sp. based on 16S rRNA analysis. This study aimed to identify antibacterial compounds produced by DM6 isolate using GC-MS. The GC-MS results showed that the chloroform extract of DM6 isolate contained : 1-Octadecene (CAS).alpha.-Octadecene, Hexanedioic acid, dioctyl ester (CAS) Dioctyl adipate, Phenol, 3,5-bis(1,1-dimethylethyl)- (CAS) 3,5-Di-tert-butylphenol, and 1,4-diaza-2,5-dioxobicyclo[4.3.0]nonane. Adipic acid/hexanedioic acid and phenols are also known to contained in the leaves of Coleus scutellariodes. This indicated that endophytic bacteria were able to synthesize antibacterial compounds which similar with the host plant.
Antioxidant Activity of Telang (Clitoria ternatea L.) Extract in Inhibiting Lipid Peroxidation Ukhradiya Magharaniq Safira Purwanto; Kamaratih Aprilia; Sulistiyani
Current Biochemistry Vol. 9 No. 1 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.1.3

Abstract

Butterfly pea (Clitoria ternatea L.) has been widely used as a healthy drink as well as traditional medicine. This study aimed to test the antioxidant activity of C. ternatea aqueous extract based on its ability to inhibit lipid peroxidation (in vitro) using different parts and extraction methods. Extraction was carried out on flowers, leaves, and roots. The leaves and roots were extracted by boiling technique. Flowers were extracted in 3 ways: boiling, maceration, and brewing. Antioxidant activity was determined based on phenolic content, flavonoid content, and antioxidant test using the TBA method. The highest phenolic and flavonoid levels were shown by the aqueous extract of C. ternatea leave of 57.51 mg GAE/g and 1.50 mg EK/g, respectively. The highest levels of flavonoid were from flower parts treated with boiling technique (0.88 mg EK/g), followed by brewed flowers and macerated flowers. The highest antioxidant activity was obtained from flowers that were boiled for 30 minutes with a percentage inhibition of 94%. Based on the method of extraction and part of the plant, all extracts of C. ternatea have similar antioxidant potential.
Analisis Profil Metabolit Sekunder Ekstrak Etanol Brokoli (Brassica oleracea var. italica) pada Pemupukan Biokonversi Maggot Black Soldier Fly Purwanto, Ukhradiya Magharaniq Safira; Sulistiyani; Azizah, Anisa Nur Wahida; Rosyidah, Rara Annisaur
Current Biochemistry Vol. 12 No. 2 (2025)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.12.2.3

Abstract

Brokoli (Brassica oleracea var. italica) merupakan sayuran fungsional yang diketahui mengandung berbagai senyawa bioaktif dengan potensi nutrisi dan farmakologis yang penting. Penelitian ini bertujuan untuk memprofilkan senyawa bioaktif ekstrak brokoli yang dibudidayakan dengan perlakuan pemupukan berbeda, yaitu kontrol, pupuk kandang, dan pupuk biokonversi maggot black soldier fly (BSF). Sampel brokoli diekstraksi menggunakan etanol 80%, kemudian dianalisis dengan LC-MS/MS untuk mengidentifikasi dan mengkuantifikasi komposisi metabolit. Hasil penelitian menunjukkan bahwa perlakuan pupuk biokonversi maggot menghasilkan pertumbuhan tanaman yang lebih baik, meliputi tinggi tanaman, jumlah daun, biomassa segar dan kering, serta persentase berbunga dibanding perlakuan lain. Selain itu, perlakuan ini juga menghasilkan metabolit yang lebih beragam dengan kelimpahan lebih tinggi, terutama golongan fenolik, terpenoid, piridazin, dan indol. Beberapa senyawa utama yang teridentifikasi antara lain Indole-3-Propionic Acid dan N(1),N(8)-Bis(Coumaroyl)Spermidine(1+), serta turunan klorofil seperti pyropheophorbide-a yang ditemukan dengan kelimpahan signifikan. Temuan ini menunjukkan bahwa pupuk biokonversi maggot BSF mampu meningkatkan pertumbuhan sekaligus profil metabolit sekunder brokoli, sehingga berpotensi sebagai strategi pemupukan ramah lingkungan untuk meningkatkan kualitas nutrisi dan fungsional tanaman ini. 
Co-Authors Abdjad Asih Nawangsih Achyar, Catellia Auliany Athaya, Ardelia Aulia Ayu Rispriandari Azizah, Anisa Nur Wahida Azizah, Annisa Nur Wahida Destiandani, Khansa DIMAS ANDRIANTO Dwi Endah Kusumawati Dwicesaria, Maheswari Alfira Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Febrina, Adella Firdaus, Muhamad Fajar Fitrilia, Tiana Gholam, Gusnia Meilin Hanifati, Qisthina Hudayanti, Martini Husnawati Husnawati Iman, Ramadhan Nur Kamaratih Aprilia MARIA BINTANG Mega Safithri Nabilla, Rahmah Nusyarah, Ayu Tri Putri, Fadhilah Eka Raisyadikara, Fadila Rini Kurniasih, Rini Rosyidah, Rara Annisaur Sari, Sintia Permata Septaningsih, Dewi Anggraini Sigiro, Ria Heni Sulistiyani