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THE SRY GENE VARIATIONS AMONGST SELECTED MADURA CATTLE POPULATIONS Prihatin, Koko Wisnu; Maylinda, Sucik; Hakim, Luqman
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.9674

Abstract

The aim of this study was to determine the diversity of the sex-determining region Y (SRY) gene in Madura cattle bulls that had specifically selected for the production of frozen semen. DNA was isolated from the whole blood derived from 5 Madura cattle bulls and the SRY gene amplification carried out using polymerase chain reaction (PCR) with SRY-4 as a primer followed by DNA sequencing. Results revealed the proximity of the Madura cattle SRY genes to the Bos indicus SRY genes, furthermore this study also proved the existence of variation in Madura cattle SRY gene caused by mutation and deletion of nucleotides. It was concluded that the variations in Madura cattle SRY genes still persist even in the specifically selected populations with similar phenotype.
REPEATABILITY ESTIMATION OF SEMEN PRODUCTION AND QUALITY OF LOCALS MADURA CATTLE BREED (Bos indicus) Prihatin, Koko Wisnu; Hakim, Luqman; Maylinda, Sucik
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i2.5942

Abstract

The objective of this study was to evaluate the effect of genetic and environmental factor on locals Madura cattle semen production and quality. A total of 2275 semen collections records from 11 bulls from 2012 to 2014 were analyzed to evaluate the genetic potency of semen productions. Genetic evaluation consists of repeatability estimation of semen volume, sperm concentration and sperm individual motility. Data were analyzed by HGLM (REML) with environmental factors of age, seasons, interval of semen collections, and frequency of ejaculations as fixed effect and bulls as random effect using GENSTAT 16th edition. Result showed that age and ejaculation frequency affect to all variables; interval of semen collections affected to both semen volume and sperm concentrations; while seasons only affected to sperm concentrations. Repeatability estimation of semen volume, sperm concentration and sperm individual motility were 0.376, 0.445 and 0.567 respectively. It was concluded that the quality of Madura bulls ejaculate less affected by environmental factors.