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Pengaruh Konsumsi Ekstrak Daun Katuk Terhadap Kecukupan ASI Pada Ibu Menyusui Di Klaten Endang Suwanti; Kuswati Kuswati
Interest : Jurnal Ilmu Kesehatan Vol 5 No 2 (2016): INTEREST : JURNAL ILMU KESEHATAN
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/interest.v5i2.42

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Abstract: Sauropus Androgynus, Breast Milk. Mother breat Milk is good nutrition for baby. Mother breast Milk important for growing baby. To increasing mother breast milk very good consumption food this like : leaf Katu (Sauropus androgynous), leaf Ubi jalar (Ipomoea batatas), leaf kelor (Moringa oleifera), fried corn at all, Composition of leaf katuk is proteins, fats, calcium, phosphor, iron, vitamins A, B, and C. pyrrolidinone, metil pyroglutamate and p-dodesilfenol component minor. Goal research to known relationship consumption leaf katuk with breast milk sufficient, at midwife practice Independent (BPM) on Klaten area. Methods: Pre-Posts with Control Group Design. In the research researcher measure influence intervention at an eksperiment group with comparing group control. Research worked at midwife practice Independent at the January as to Juli 2015. Population in the research is all of the mother breastfeeding at midwife practice Independent (BPM) on Klaten district. Sampling methods is quota sampling with inclusive criterion normally newborn and healthy. Research at January-July 2015. Data analysis performed to describe the variable that will be studied and performed bivariate analyze to the relationship of independent and dependent variable s using the chi-square. Results research is 70 % intervention group more produced milk than control group produced milk enough 30%. Result in statistik Analice chi-square p-value =0,002.Conclusion there was significant relationship consumption extract katuk for sufficient breast milk.
Peningkatan Kecepatan Pengeluaran Kolostrum Dengan Perawatan Totok Payudara Dan Pijat Oksitosin Pada Ibu Post Partum Kuswati Kuswati; Henik Istikhomah
Interest : Jurnal Ilmu Kesehatan Vol 6 No 2 (2017): INTEREST : JURNAL ILMU KESEHATAN
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/interest.v6i2.106

Abstract

Abstract: Totok Payudara, Oxytocin Massage And Colostrum. The purpose of this study was to determine differences in time of leaking colostrums in mothers post partum in doing oxytocin massage with totok payudara. This study design used is quasiexperimental design with the design Nonequivalent Control Group Design. Reserch conducted at the midwives (BPM) at health centers working area Klaten who were divided into 2 groups, i.e an experimental group consisting of 30 respondens to whom oxytocin massage was given and a control group consisting of 30 responden to whom totok payudara was given. Bivariate data analysis using statistical test used is t –test. Results of the study showed the average time of leaking colostrums in experimental and control groups were 152,67 minutes and 137,23 minutes. The result of hypothesis test using T-test obtained the value of p= 0.668 (p>0.05). Conclusion: there is no difference time between oxytocin massage and totok payudara to the leaking colostrum
Kualitas Semen dengan Berbagai Formulasi Pengencer Dasar Air Kelapa Hijau Selama Simpan Dingin pada Sapi Madura Aulia Puspita Anugra Yekti; Jois Harsah; Muchamad Luthfi; Muhammad Dikman; Asri Nurul Huda; Kuswati Kuswati; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 3 (2018): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.732 KB) | DOI: 10.33772/jitro.v5i3.4738

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ABSTRAKInseminasi Buatan dengan menggunakan semen cair digunakan untuk daerah yang sulit nitrogen cair dan mempunyai kualitas yang lebih baik dari pada semen beku. Tujuan penelitian ini adalah untuk mengetahui kualitas berbagai bahan pengencer dasar air kelapa penyimpanan dingin 2-5°C.Penelitian ini dilaksanakan di Loka Penelitian Sapi Potong Grati, Pasuruan. Semen yang digunakan berasal dari sapi madura sebanyak dua ekor, berumur 5 dan 3 tahun  dan berat badan yaitu 397 kg dan 360,5 kg. Sapi madura ditampung seminggu 2 kali dengan motilitas > 70% , sedangkan air kelapa yang digunakan adalah air kelapa hijau yang masih muda. Pengenceran semen cair dibagi menjadi 4 yaitu P0 (CEP-3 + 20% kuning telur) sebagai kontrol, P1 (air kelapa hijau +20% kuning telur), P2 (P1 + 0,4% putih telur + 1% fruktosa) dan P3 (P1 + 0,4% putih telur kuning telur +2% fruktosa). Data dianalisis menggunakan uji Pearson’s Chi Square dan Uji Deskriptif. Hasil penelitian menunjukkan motilitas spermatozoa sesuai standar SNI yaitu motilitas> 40% pada pengencer CEP-3 dapat disimpan selama hari ke-8 (40,50±6,43%) sedangkan pada pengencer dasar air kelapa hijau pada P1, P2 dan P3 tidak menunjukkan perbedaan yang nyata (P>0,05) selama disimpan 6 hari yaitu 40,50±10,12%, 38,00±4,22%, 40,00±8,50%. Abnormalitas dari semua perlakuan menunjukan nilai <20%. Viabilitas didapatkan nilai tertinggi pada perlakuan P0(89,58±2,16%) kemudian P1(89,39±3,79%), P2(88,62±4,59%) dan P3(87,93±4,41%).Kata kunci: CEP-3, semen cair, sapi madura, simpan dingin, air kelapa hijau ABSTRACTArtificial Insemination using liquid semen is performed for areas that where liquid nitrogen is difficult to find and havng better quality than frozen semen. Purpose of this research was to investigate the quality on various coconut water base diluents on liquid semen of madura bull during cold storage of 2-5°C. Research was conducted at Laboratory of Reproduction of Grati Beef Cattle Research Station, Pasuruan.Semen that is used comes from two madura bulls aged 5 and 3 years with body weight is 397 kg and 360.5 kg. The semen was collected twice a week with motility> 70%, and the coconut water used is unripe green coconut water. The research treatments were P0 (CEP-3 + 20% egg yolk) as control, P1 (unripe green coconut water + 20% egg yolk), P2 (P1+ 1% fructose + 0.4% egg white) and P3 (P1+ 0.4% egg white + 2% fructose). Data were analyzed using Pearson's Chi Square test and Descriptive Test. The results showed that the motility of spermatozoa was within Indonesian National Standard (SNI) with more than 40% motility in the CEP-3 diluent and it can be stored until the 8th day (40.50 ± 6.43%). The basic diluents of green coconut water at P1, P2 and P3 was not significantly affected (P> 0.05) until 6 days storing with the motility number average are 40.50 ± 10.12%, 38.00 ± 4.22%, 40, 00 ± 8.50%. The abnormality of all treatments was under 20%. The highest viability was showed by treatment P2 (89.58±2.16%), followed by P4 (89.39 ± 3.79%), P3 (88.62 ± 4.59%) and the lowest was P4 (87.93 ± 4.41%). Keywords:CEP-3, liquid semen, madura bull, cool storage, green coconut water ABSTRACT               Artificial Insemination using liquid semen is performed for areas that where liquid nitrogen is difficult to find and havng better quality than frozen semen. Purpose of this research was to investigate the quality on various coconut water base diluents on liquid semen of madura bull during cold storage of 2-5°C. Research was conducted at Laboratory of Reproduction of Grati Beef Cattle Research Station, Pasuruan.Semen that is used comes from two madura bulls aged 5 and 3 years with body weight is 397 kg and 360.5 kg. The semen was collected twice a week with motility> 70%, and the coconut water used is unripe green coconut water. The research treatments were P0 (CEP-3 + 20% egg yolk) as control, P1 (unripe green coconut water + 20% egg yolk), P2 (P1+ 1% fructose + 0.4% egg white) and P3 (P1+ 0.4% egg white + 2% fructose). Data were analyzed using Pearson's Chi Square test and Descriptive Test. The results showed that the motility of spermatozoa was within Indonesian National Standard (SNI) with more than 40% motility in the CEP-3 diluent and it can be stored until the 8th day (40.50 ± 6.43%). The basic diluents of green coconut water at P1, P2 and P3 was not significantly affected (P> 0.05) until 6 days storing with the motility number average are 40.50 ± 10.12%, 38.00 ± 4.22%, 40, 00 ± 8.50%. The abnormality of all treatments was under 20%. The highest viability was showed by treatment P2 (89.58±2.16%), followed by P4 (89.39 ± 3.79%), P3 (88.62 ± 4.59%) and the lowest was P4 (87.93 ± 4.41%).
KUALITAS DAN KAPASITASI SPERMATOZOA SAPI BALI, MADURA, DAN PERANAKAN ONGOLE Aulia Puspita Anugra Yekti; Willy Saputra Saputra Tatulus; Dian Ratnawati; Luqman Affandhy; Kuswati Kuswati; Asri Nurul Huda; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 2 (2018): JITRO, Mei
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.664 KB) | DOI: 10.33772/jitro.v5i2.4739

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ABSTRAKTujuan penelitian ini adalah untuk mengetahui perbedaan kualitas dan kapasitasi spermatozoa pada sapi potong lokal. Selain itu, penelitian ini juga diharapkan dapat digunakan sebagai standar uji kualitas semen. Bahan yang digunakan dalam penelitian ini adalah dua ekor sapi jantan ongole, sapi bali dan sapi madura. Hasil penelitian  menunjukkan bahwa persentase motilitas spermatozoa sapi bali 70,83±2,04%, sapi madura 70,00±0,00% dan sapi PO 71,67±2, 58%. persentase Viabilitas spermatozoa sapi bali adalah 89,39±2,84%, sapi madura 90,60 ± 3,13% dan sapi PO 92,13±2,08%. Persentase abnormalitas spermatozoa sapi bali adalah 3,48±1,09%, sapi madura 2,13±0,86% dan sapi PO 2,86±0,51%. Konsentrasi sperma sapi bali 1126,67±169,08 juta/mL, sapi madura 1076,67±3,94 juta/mL dan sapi PO 1210±160,87 juta/mL. Total spermatozoa motil pada sapi bali adalah 3136,9±653,4 juta/mL, sapi madura 3520,41±357,48 juta dan sapi PO 3653,83±1293,59 juta/mL. Persentase status akrosom spermatozoa adalah 85,72±1,72%, sapi madura 85,35±0,76% dan sapi PO 86,40±1,97%. Data yang didapatkan dalam penelitian ini dianalisis menggunakan rancangan acak lengkap dimana hasil analisa menunjukkan tidak ada perbedaan kualitas yang signifikan (P>0,05). Dapat disimpulkan perbedaan jenis pada sapi lokal tidak berpengaruh pada kualitas dan kapasitas spermatozoa, tetapi sapi PO memiliki persentase yang lebih tinggi dari kualitas dan kapasitasi sperma dibandingkan sapi bali dan sapi madura.Kata kunci : kualitas semen, spermatozoa, kapasitasi, sapi lokalABSTRACTThe purpose of this research was to determine the differences in the quality and capacitation of local beef cattle sperm. In addition, this study was also expected to be used as a standard of semen quality test. The material used in this study were two bulls of crossbreed ongole, bali cattle and madura cattle. The results of this study revealed that percentage of motility  sperm bali cattle  70.83 ± 2.04%, madura cattle 70.00 ± 0.00% and PO cattle 71.67 ± 2, 58%. percentage of  Viability sperm of bali cattle was 89.39 ± 2.84, madura cattle 90.60 ± 3.13% and PO cattle 92.13 ± 2.08%. Percentage of abnormality sperm bali cattle  were 3.48 ± 1.09%, madura cattle 2.13 ± 0.86% and PO cattle 2.86 ± 0.51%. percentage of concentration sperm bali cattle 1126.67 ± 169.08 million / mL, madura cattle 1076.67 ± 73.94 million / mL and PO cattle 1210 ± 160.87 million / mL. percentage of  total motile sperm bali was 3136.9 ± 653.4 million / mL, madura cattle 3520.41 ± 357.48 million and PO cattle 3653.83 ± 1293.59 million / mL. percentage of status acrosom sperm is 85.72 ± 1.72%, madura cows 85.35 ± 0.76 and cattle PO 86.40 ± 1.97. Data of this research was analyzed using Block  Randomized Design (BRD) which showed not  significant differences in quality (P> 0.05). The conclusion based on the observations made in this research, differences in local cattle have no effect on the quality and capacitation of spermatozoa, but cross breed ongole cattle have a higher percentage of quality and capacitation of sperm than bali cattle and madura cattle. Semen of bali cattle, and madura cattle used in this study can be used for artificial insemination.Keywords: semen quality, sperm capacitation, local cattle 
Penampilan Produksi Sapi Aceh Umur Satu Hari, Umur Sapih, dan Umur Satu Tahun Ikhsanuddin Ikhsanuddin; V.M. Ani Nurgiartiningsih; Kuswati Kuswati; Mukhtar Mukhtar
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 3 (2018): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.785 KB) | DOI: 10.33772/jitro.v5i3.4885

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ABSTRAKPenelitian ini bertujuan untuk mengetahui penampilan produksi sapi aceh di BPTU-HPT Indrapuri Aceh. Materi penelitian yaitu sapi aceh umur satu hari, umur sapih, dan umur satu tahun sebanyak 159 ekor. Metode penelitian adalah studi kasus berdasarkan data bobot lahir, bobot sapih, bobot satu tahun beserta ukuran tubuhnya. Data dianalisis menunjukkan bahwa sapi jantan memiliki bobot lahir 13,66±1,08 kg, bobot umur 205 hari 71,60±7,92 kg dan bobot  umur 365 hari 104,66±11,72 kg.  Sapi betina memiliki bobot lahir 13,88±1,32 kg, bobot umur 205 hari 64,38 ± 10,36 kg dan bobot umur 365 hari 90,29±11,95 kg. Sapi Aceh umur satu hari memiliki lingkar dada, panjang badan dan tinggi pundak berturut-turut  55,41 cm, 46,16 cm  dan 55,22 cm pada sapi jantan serta 55,87 cm, 46,83 cm, dan 55,74 cm pada sapi betina. Sapi aceh umur 365 hari memiliki ukuran lingkar dada, panjang badan dan tinggi pundak berturut-turut 114,32 cm, 94,31 cm dan 92,93 cm pada sapi jantan serta 104,25 cm, 85,06 dan 87,71 cm pada sapi betina. Rata-rata penampilan produksi sapi aceh di BPTU Indrapuri  berpotensi menjadi ternak unggul berdasarkan kriteria SNI.Kata Kunci: sapi aceh, penampilan produksi, bobot badan, ukuran tubuhABSTRACTThis study aimed to evaluate the productive performance of aceh cattle at Indrapuri Breeding Center for Superior Livestock and Forage. A total of 159 heads of aceh cattle were used in this study. The method used was a case study based on the data of birth weight, weaning weight, yearling weight, and body size. The results showed that the birth weight, weaning weight at 205 days of age, and yearling weight at 365 days of age of male aceh cattle were 13.66±1.08 kg, 71.60±7.92 kg, and 104.66±11.72 kg, respectively. Whereas, female aceh cattle had the birth weight, weaning weight at 205 days of age, and yearling weight at 365 days of age of 13.88±1.32 kg, 64.38±10.36 kg, and 90.29±11.95 kg, respectively. At birth, male Aceh cattle had the chest circumference, body length, and shoulder height of 55.41 cm, 46.16 cm, and 55.22 cm, respectively, while female aceh cattle were 55.87 cm, 46.83 cm, and 55.74 cm, respectively. At 365 days of age, male aceh cattle had the chest circumference, body length, and shoulder height of 114.32 cm, 94.31 cm, and 92.93 cm, respectively, while female aceh cattle were 104.25 cm, 85.06 cm, and 87.71 cm, respectively. The average productive performance of aceh cattle at Indrapuri Breeding Center for Superior Livestock and Forage has the potential to become superior cattle based on the criteria of Indonesian National Standard.Keywords: aceh cattle, performance, body weight, body size
Kapasitasi Spermatozoa Sapi Peranakan Ongole dalam Berbagai Formulasi Pengencer Air Kelapa Selama Simpan Dingin Dedi Muhammad; Nurul Isnaini; Kuswati Kuswati; Aulia Puspita Anugra Yekti; Muchamad Luthfi; Lukman Affandhy Sunarto; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.06 KB) | DOI: 10.33772/jitro.v6i1.5450

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ABSTRAKPengencer dasar air kelapa merupakan solusi dari sulit dan mahalnya harga pengadaan bahan baku pembuatan pengencer. Tujuan dari penelitian ini adalah untuk mengetahui kapasitasi spermatozoa sapi Peranakan Ongole dalam berbagai formulasi pengencer air kelapa selama simpan dingin pada suhu 4-5oC. Metodologi yang digunakan adalah eksperimantal laboratorium menggunakan rancangan acak kelompok (RAK). Terdapat tiga perlakuan yaitu, P1 = Pengencer air kelapa + 20% kuning telur, P2 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 1000 mg/l. P3 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 2000 mg/l, dengan 10 ulangan. Hasil menunjukkan bahwa tidak terdapat perbedaan yang signifikan pada persentase spermatozoa belum kapasitasi, terkapasitasi dan telah rekasi akrosom antara perlakuan formulasi pengencer air kelapa yang berbeda (P1, P2, P3) selama peyimpana suhu 2-5oC. Pengencer berbasis air kelapa mampu mempertahankan kualitas akrosom diatas 50% penyimpanan hari ke lima.Kata kunci : air kelapa, kapasitasi, reaksi akrosom, semen cairABSTRACTCoconut water extender is the solution to the difficulty and high cots of semen diluents. The objective of this study were to examine the capacitation of Ongole Crossbreed sperm in various formulations of coconut water extender during storage in 4-5 oC. The study method used laboratory experimental. The experimental design was Randomized Completely Block Design and the data were analyzed by Analyze of Variance. There are three treatments in this study (P1 = Coconut Water + 20% of yolk, P2 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 1 mg/ml, dan P3 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 2 mg/ml) and ten replications of each treatment. There were not significant differences in percentage of sperm uncapacitation, sperm capacitaion and sperm acrosom reaction between various formulations of coconut water diluents during chilled storage at 4-5 oC. Coconut water diluents are able to maintain quality of sperm acrosom up to day 5th with values above 50%.Keywords: acrosom reaction, capacitation, coconut water, liquid semen
Kualitas Semen Beku Sexing Sapi Peranakan Ongole Menggunakan Volume Semen Awal Yang Berbeda Irvan Mardi; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Muchamad Luthfi; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 7, No 3 (2020): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.022 KB) | DOI: 10.33772/jitro.v7i3.12203

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ABSTRAKInseminasi buatan dengan menggunakan semen sexing diharapkan menghasilkan pedet dengan jenis kelamin sesuai harapan. Tujuan dari penelitian ini adalah untuk mengetahui kualitas, proporsi, dan jumlah produksi straw sexing menggunakan metode sentrifugasi gradien densitas percoll dengan volume awal semen yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong, Kecamatan Grati, Kabupaten Pasuruan dan Laboratorium Reproduksi Ternak, Fakultas Peternakan Universitas Brawijaya, Malang. Materi yang digunakan adalah semen sapi peranakan ongole berumur berkisar lima tahun dan bobot badan  berkisar 700 kg sebanyak tiga ekor, motilitas massa ≥2+ dan motilitas individu ≥70%. Metode yang digunakan adalah eksperimental dengan tiga perlakuan volume awal saat sexing, yaitu 1 (P1); 1,5 (P2); dan 2 (P3) ml dengan ulangan 11 kali (ulangan berfungsi sebagai kelompok). Data dianalisa menggunakan Rancangan Acak Kelompok (RAK). Hasil penelitian menunjukkan bahwa perbedaan volume awal semen tidak berpengaruh (menurun) terhadap motilitas, viabilitas, abnormalitas, konsentrasi, total spermatozoa motil, recovery rate dan proporsi spermatozoa (P>0,05). Pengaruh yang sangat nyata (meningkat) terhadap jumlah produksi straw (P<0,01). Ulangan penelitian ini memberikan pengaruh yang sangat nyata (meningkat) terhadap kualitas (motilitas, konsentrasi, viabilitas, abnormalitas, total spermatozoa motil, RR, proporsi dan jumlah straw) dan proporsi spermatozoa X dan Y (P<0,01). Total spermatozoa motil setiap perlakuan telah memenuhi nilai harapan (10 juta/straw). Proporsi spermatozoa X dan Y telah memenuhi nilai harapan (80%:20%).Kata Kunci: kualitas, proporsi, semen beku sexing, strawABSTRACTArtificial insemination using sexing semen is expected to produce calves with the expected sex. The aim of this study was to determine the quality, proportion, and quantity of sexing semen production using the percoll density gradient centrifugation method with different initial semen volumes. The research was conducted at the Beef Cattle Research, Grati District, Pasuruan Regency, East Java Province, Indonesia, and the Animal Reproduction Laboratory, Faculty of Animal Science, University of Brawijaya, Malang, East Java Province, Indonesia. The material used was semen from three Ongole crossbred bull aged around five years and the bodyweight of around 700 kg, mass motility of ≥2+, and individual motility ≥70%. The method used was experimental with three initial volume treatments during sexing, namely 1 (P1); 1.5 (P2), and 2 (P3) ml with 11 replications (replications function as groups). The data were analyzed using a randomized block design (RBD). The results showed that the treatment of differences in initial semen volume did not affect motility, viability, abnormalities, concentration, total motile sperm, recovery rate, and proportion of sperm (P>0.05). On the other hand, the difference in the initial volume of semen had a very significant effect (increased) on the amount of frozen semen production (P<0.01). Repeated research also had a very significant effect (increased) on the semen quality (motility, concentration, viability, abnormality, total sperm motility, recovery rate proportion, and straw production) and the proportion of spermatozoa X and Y (P<0.01). The total motile sperm for each treatment had met the expected value (10 million/straw). Proportions of spermatozoa X and Y have met the expected value (80%: 20%).Keywords: proportion, quality, sexing frozen semen, straw.
Filogeni Beberapa Sapi Lokal Indonesia Menggunakan DNA Mitokondria COI (Cytochrome Oxidase Sub unit I) Anisa Wulandari; V.M. Ani Nurgiartiningsih; Kuswati Kuswati; Tri Eko Susilorini; Paskah Partogi Agung
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 2 (2019): JITRO, Mei
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.833 KB) | DOI: 10.33772/jitro.v6i2.6165

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ABSTRAKTujuan penelitian ini adalah untuk mendapatkan konstruksi pohon filogeni berdasarkan keragaman DNA Mitokondria khususnya COI (Cytochrome Oxidase Sub unit I). Sampel yang digunakan yakni darah sapi PO, DNA sapi SO, sapi pesisir, sapi bali, sapi madura dan sapi pasundan masing-masing 12 sampel. Sekuen COI dianalisis menggunakan MEGA 7.0 software dengan metode bootstrapped Neighboor-Joining (NJ) 1000 kali pengulangan berdasarkan between group untuk merekonstruksi pohon filogeni. Hasil penelitian menunjukkan sapi pesisir dekat dengan sapi SO, sapi madura dan sapi pasundan tidak memiliki kekerabatan dengan sapi lokal Indonesia lainnya sehingga membentuk garis tersendiri dan sapi PO dekat dengan sapi bali.Kata Kunci: COI, DNA mitokondria, filogeni sapi lokal indonesiaABSTRACTThe purpose of this study was to obtain the construction of phylogeny trees based on the diversity of Mitochondrial DNA especially COI. The samples used were PO cattle blood, SO cow DNA, Coastal cattle, bali cattle, madura cattle, and pasundan cattle every 12 samples. COI sequences were analyzed using MEGA 7.0 software with the bootstrapped Neighbor-Joining (NJ) method 1000 repetitions based on the between groups to reconstruct the phylogeny tree. The results showed that boastal cattle close to SO cattle, madura cattle, and pasundan cattle did not have a kinship with other local Indonesian cows to form a separate line and PO cattle close to bali cattle.Keywords: mitochondrial DNA, coi, indonesian local cow, phylogeny
Perbedaan Tingkat Kecemasan Ibu Bersalin Pada Kelas Ibu Hamil Di Wilayah Kerja Puskesmas Tulung Intan Nugraheni; Kuswati Kuswati
Jurnal Kebidanan dan Kesehatan Tradisional Vol 2 No 2 (2017): Jurnal Kebidanan dan Kesehatan Tradisional
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/jkkt.v2i2.49

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Abstract: Class Pregnant Women; Levels Of Anxiety. Anxiety is one of the main factors that influence whether or not an action smoothness of delivery. Pregnant women with sufficient knowledge proved to have a low risk of the occurrence of complications of childbirth. Educational intervention in the form of counseling mothers on maternal class. Purpose is to knowing the differences in levels of maternal anxiety on the class of pregnant woman in the Puskesmas Tulung. Type of quasi-experimental research design with a static group comparison. The population is pregnant women PHC Tulung region. Samples are 36 pregnant women who meet the criteria cadre, which consisted of 18 pregnant women who attend classes pregnant women and 18 pregnant women who donot attend classes pregnant women with purposive sampling technique. Respondents who attend classes maternal anxiety has a value of at least 6, the maximum value of 20 with an average of 15.4 and the majority of respondents (61.1%) had anxiety medium category. Those who do not attend classes anxiety mother has a value of at least 14, the maximum value of 27, with an average of 20.67 and a majority of respondents have anxiety was (88.9%). There are differences in the level of maternal anxiety that follows and that pregnant women do not attend classes in the Puskesmas Tulung, with significant differences in anxiety score (p value = 0.000).
Hubungan Konsumsi Ekstrak Daun Kentang Manis Dengan Produksi ASI Di Laktasi Ibu Di Kabupaten Klaten Endang Suwanti; Kuswati Kuswati
Jurnal Kebidanan dan Kesehatan Tradisional Vol 1 No 2 (2016): Jurnal Kebidanan dan Kesehatan Tradisional
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/jkkt.v1i2.72

Abstract

Absctract : Sweet Potatoes Leaf - Breast Milk Production. Breast Milk is a good nutrition for baby. Breast milk important for growing babies. To increase breast milk it is important for mother to consume food like: Katu leaf (sauropus androgynus), sweet potatoes leaf (Ipomoe batatas), Kelor Leaf ( moringa oleifera), fread corn etc. The composition of sweet potatoes leaf is low fat and cholesterol. This leaf is source of proteins, calcium, niacin and iron. Sweet potatoe leaf also contain high fibre, pro vitamins A, vitamin C, riboflamin, vitamin B6, folat, magnecium fosfor, potassium and mangan. It increases breast milk because this leaf contains lagtagagum. The goal of this research is to know the relationship between the consumption sweet potatoes leaf wit breast milk production on lactation mother at Independent Midwife Practice Indarwati, Mranggen and Siti Sujalmi, Socokangsi, Jatinom, Klaten. Methods : Pre=Post test with Control Group. Design : this research measure the influence between experiment and control group during September 2014 until February 2015. Population in this research is all breastfeeding mothers. Sample was taken wih quota sampling wit 15 mothers for intervention group and 15 mothers for control group with inclusive criterion is mothers with normal delivery and healthy newborn babies. Univariat analysis performed to describe the variable with the result is 73.3 % intervention group more produced breast milk compared to control group 13.3%. Bivariat analize to know the relationship between independent and dependent variable by using Pearson Correlation with the statistic result p value= 0.000. Conclusion, There was significant relationship between the consumption sweet potatoes leaf for increasing breast milk production