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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Media Penelitian dan Pengembangan Kesehatan Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Biotek Medisiana Indonesia Medical Journal of Indonesia Sari Pediatri Paediatrica Indonesiana Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) The Indonesian Journal of Infectious Diseases Eksakta : Berkala Ilmiah Bidang MIPA Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Makara Journal of Science Journal of Clinical Microbiology and Infectious Diseases Jurnal Kefarmasian Indonesia
Fera Ibrahim
Departemen Mikrobiologi Fakultas Kedokteran Universitas Indonesia/KSM Mikrobiologi Klinik RSUPN Dr Cipto Mangunkusumo, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Public Health

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Journal : Paediatrica Indonesiana

 1 
Difference of hemaglutinins between wild-type and vaccine measles virus in Indonesia Made Setiawan; Agus Sjahrurachman; Fera Ibrahim; Agus Suwandono
Paediatrica Indonesiana Vol 48 No 1 (2008): January 2008
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (465.242 KB) | DOI: 10.14238/pi48.1.2008.42-8

Abstract

Background Hemaglutinin (H) protein of measles virus is veryimportant in the process of host cell infection. H protein is alsoable to induce specific antibodies which can neutralize measlesvirus and block the cell infection.Objective This study aimed to explore the nucleotide and aminoacid sequence differences between wild-type measles virus (G2,G3 and D9) with CAM-70, Schwarz and Edmonston-wt vaccinevirus.Methods The exctration and amplification of the gene wereconducted in the laboratory using biomolecular technology. Thegene and protein analysis were conducted using the bioinformatictechnology.Results The results showed that the differences in nucleotidesequences were highest between wild-type virus and CAM-70vaccine virus (76-77 nucleotides), followed by Schwarz (61-64nucleotides) and Edmonston (60-63 nucleotides). The differencesin amino acid sequences were highest between wild-type virusand CAM-70 (24-29 residues), followed by Schwarz (13-20residues) and Edmonston (12-19 residues).Conclusion The Indonesian wild-type measles virus was geneticallycloser to Schwarz vaccine virus than CAM-70 vaccine virus,hence the neutralizing antibodies generated by Schwarz vaccinewere more specific against Indonesian wild-type virus comparedto CAM-70 vaccine.
Differences of nucleotide and amino acid sequences of nucleoprotein (N) gene between wild-type measles virus and vaccine virus in Indonesia Made Setiawan; Agus Sjahrurachman; Fera Ibrahim; Agus Suwandono
Paediatrica Indonesiana Vol 48 No 2 (2008): March 2008
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.334 KB) | DOI: 10.14238/pi48.2.2008.81-7

Abstract

Background Measles virus is a member of genus morbiliviruswhich belongs to family paramyxovirus with negative, single-strand RNA genome. RNA is packed by nucleocapsid (N) protein.The N protein is very important for RNA replication andtranslation. Abnormality in N protein will induce abnormality invirus replication.Objective This study aimed to explore the differences ofnucleotide sequence of N gene and amino acid sequences of Nprotein between wild-type measles virus (G2, G3 and D9) andvaccine virus (CAM-70, Schwarz and Edmonston-wt)Methods The exctraction and amplification of the gene wereconducted in the laboratory using biomolecular technology. Thegene and protein analysis were conducted using the bioinformatictechnology.Results The results showed that more differences were foundbetween nucleotide sequences of N gene of wild-type measlesvirus against CAM-70 vaccine virus (77 – 79 nucleotides)compared against Schwarz and Edmonston-wt vaccine virus (71-74 nucleotides). Likewise, more differences were also observedbetween amino acid sequences of N protein of wild-type measlesvirus against CAM-70 vaccine virus (18-24 residues) comparedagainst Schwarz and Edmonston-wt vaccine virus (17-23 residues).
Critical site differences of fusion protein between wildtype and vaccine measles virus strains in Indonesia Made Setiawan; Agus Sjahrurachman; Fera Ibrahim; Agus Suwandono
Paediatrica Indonesiana Vol 51 No 3 (2011): May 2011
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.395 KB) | DOI: 10.14238/pi51.3.2011.123-7

Abstract

Background Measles virus can cause high morbidity and mortality in infants and children. Fusion glycoprotein (F protein) found in the viral envelope is important for the host cell infection mechanism. F protein is immunogenic and may cause specific immune responses in the host. High variability is found in the F protein gene of vaccine viral strains compared to 'Wild type strains. This amino add sequence variability may result in a less specific immune response against other strains, possibly rendering thevaccine to be less effective.Objective To detennine the amino add sequence differences in critical sites of F protein in Mld type and vaccine measles virus strains in Indonesia.Methods We compared amino acid sequences of three genotypes of Mld type measles virus (02, 03 and D9) to those of the vaccine strains, CAM􀀸 70, Schwarz, and Edmonston􀀸wt type measles virus.Resul ts Analysis showed that there were differences at Fl􀀸F2 cleavage site, B cell epitopes, and H protein binding site between the CAM􀀸70 vaccine viral strains and Mld type strains. Schwarz vaccine strain differed from the wild type strains at the H protein binding site. A 03 wild type strain potential glycosylation site was also different from all other strains studied.Conclusion There were differences in the critical sites of F protein between Mld type strains and the CAM􀀸70 and Schwarz vaccine strains. 
Antigenic differences between wildtype measles viruses and vaccine viruses in Indonesia Made Setiawan; Agus Sjahrurachman; Fera Ibrahim; Agus Suwandono
Paediatrica Indonesiana Vol 48 No 3 (2008): May 2008
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.696 KB) | DOI: 10.14238/pi48.3.2008.125-35

Abstract

Background Measles virus has a single, negative strand RNAgenome which codes 6 structural proteins: N, F, P M, H and L.Currently there are several variances in the nucleotide sequencesof N, F, M and H genes across wild type measles viruses, hencemeasles viruses can be categorized into clades and genotypes. Theantigenicity of the previous genotype of measles is different fromthe current genotype.Objective To determine the antigenic differences between wildtype measles virus and measles vaccine virus.Methods Analysis of the antigenic differences between wild typevirus (G2, G3 and D9) and vaccine virus (CAM-70 and Schwarz)was performed by immunizing mice with the respective viruses.The serum was then tested with micro-cross-neutralizationtechnique using the G2, G3, D9 and CAM-70 virus. Tests withcross ELISA examination technique were also performed usingthe same set of virus.Results Analysis of the cross neutralization test and cross ELISAshowed that the highest antigenicity reaction was found betweenwild type virus with antibody against wild type virus, while thelowest reaction was between wild type virus with antibody againstCAM-70.Conclusions We conclude that the antigenicity of antigenic proteinfrom wild type virus is higher than antigenicity of vaccine virusprotein. In addition, it was found that the antigenicity of proteinsfrom Schwarz vaccine virus was higher than proteins CAM-70vaccine virus.
Co-Authors . Andriansjah . DARMINTO . DARMINTO Ade P.R. Simaremare Agus Sjahrurachman Agus Sjahrurachman Agus Sjahrurachman Agus Sjahrurachman Agus Suwandono Agus Suwandono Agus Suwandono Agus Syahrurachman Akrom, Akrom Amin Soebandrio Andi Yasmon Andrijono Andrijono Andrijono Andrijono Anna, Shoffiana Noor Ardiana Kusumaningrum Ariyani Kiranasari Aroem Naroeni Aroem Naroeni Atna Permana Beti Ernawati Dewi Betty Ernawati Budiman Bela Cliff Clarence Haliman Conny R Tjampakasari Dimas Seto Prasetyo Elisna Syahruddin Endang R. Sedyaningsih Fithriyah Fithriyah Fithriyah Fithriyah Gatot Purwoto GINA SAMAAN Hartiyowidi Yuliawuri Herna Herna Herna, Herna Iin Maemunah Ketut Tuti Parwati Krisna N.A. Pangesti Lola Febriana Dewi Louisa Ivana Utami Made Setiawan Made Setiawan Made Setiawan Mardiastuti H Wahid Mardiastuti Mardiastuti Melinda Remelia Mirna Robert D.R. van Beest Holle Muhammad F Aziz Muhammad F Aziz Mursinah Mursinah Mursinah Mursinah Mursinah, Mursinah NI LUH PUTU INDI DHARMAYANTI Ni Luh Putu Indi Dharmayanti NI LUH PUTU INDI DHARMAYANTI Ni Made Adi Tarini Ni Nengah Dwi Fatmawati Pratiwi Pujilestari Sudarmono Rela Febriani RISA INDRIANI Samsuridjal Djauzi Scania, Alifah Evi Silvia Tri Widyaningtyas Simon Yosonegoro Liem Subangkit Subangkit T Mirawati Sudiro Tiffani, Wely L Tjahjani M. Sudiro Tjahyani M. Sudiro Tofan W Utami Tofan W Utami VIVI SETIAWATY Vivi Setiawaty Wely L Tiffani Yeva Rosana Yulia Rosa Saharman YULIANTY MUHAYAR Yuliar Budi Hartanto Yuyun Soedarmono