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All Journal Jurnal Kesehatan Masyarakat Health Science Journal of Indonesia Paediatrica Indonesiana YUSTISI Unes Law Review Jurnal Ilmu Keperawatan Jiwa Yustisi: Jurnal Hukum dan Hukum Islam Indian Journal of Forensic Medicine & Toxicology PROFICIO: Jurnal Pengabdian Masyarakat Jurnal Pengabdian Kepada Masyarakat Journal of Law, Poliitic and Humanities JUSTICES: Journal of Law Jurnal Kabar Masyarakat TONGKONAN: Jurnal Pengabdian Masyarakat Jaksa: Jurnal Kajian Ilmu Hukum Dan Politik Amandemen: Jurnal Ilmu Pertahanan, Politik dan Hukum Indonesia

Agus Suwandono

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Author-ID : 3161782

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Laboratory preparedness to support the Covid-19 pandemic respond in Indonesia Ketut Aryastami; Harimat Hendarwan; Vivi Setiawaty; Amir Su'udi; Ully Adhie Mulyani; Made Dewi Susilawati; Syachroni Syachroni; Nelly Puspandari; Agus Suwandono
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.4089

Latar belakang: Penyakit jenis baru COVID-19 yang disebabkan oleh virus corona menjadi sebuah pandemic di akhir tahun 2019. Kota Wuhan (China) merupakan lokasi pertama terdeteksinya kasus COVID-19. Tanpa adanya kecurigaan apapun penyakit ini dengan cepatnya menyebar ke seluruh dunia mengikuti alur mobilitas manusia. Dalam kondisi tersebut sistem kesehatan di setiap negara tampak kelabakan khususnya dalam pengendalian transmisi penyakit. Studi ini ingin mengidentifikasi kesiapan jejaring laboratorium kesehatan di Indonesia. Metode: Penilaian cepat dilakukan terhadap ketersediaan dan kesiapan laboratoriaum dalam pennanganan pandemi Covid-19. Pengumpulan data dilakukan melalui pengisian questioner yang dikirim secara elektronik. Waktu pelaksanaan adalah minggu ketiga dan keempat, Maret 2020. Terdapat 44 laboratorium jejaring laboratorium dibawah Kementerian Kesehatan yang menjadi subjek penelitian, dan sebanyak 33 yang merespon secara lengkap Variabel ketersediaan, kecukupan dan kebutuhan bahan dan alat. Hasil: Jejaring laboratorium kesehatan dibawah Kementerian Kesehatan sudah terbentuk sejak tahun 2009. Dengan terjadinya pandemic COVID-19 Surat Keputusan Menteri Kesehatan telah direvisi hingga dua kali agar dapat meningkatkan kapasitas dan memperluas jejaring ke seluruh wilayah NKRI. Hasil studi menunjukkan, laboratorium jejaring dibawah Kementerian Kesehatan belum siap dalam menghadapi pandemic COVID-19. Dua jenis laboratorium jejaring yaitu laboratorium surveillans maupun laboratorium diagnostic memiliki kondisi yang sama. Ketersediaan bahan dan alat laboratorium standar masih tergolong rata-rata, bahkan dari sisi kecukupannyapun masih jauh dibawah kapasitas kebutuhan dalam penanganan specimen COVID-19. Kondisi yang sama juga tampak untuk bahan pendukung laboratorium termasuk alat pelindung diri untuk petugas. Kesimpulan: Kesiapan laboratorium sebagai bagian dari system kesehatan dalam kondisi pandemic masih lemah. Keberadaan alat penunjang diagnose khususnya untuk penyakit menular harus dilengkapi sesuai dengann type laboratorium. Pandemi COVID-19 menjadi alarm dalam menghadapi era baru dan antisipasi masalah dimasa yang akan datang. Kata kunci: Kesiapan laboratorium, COVID-19, Indonesia Abstract Background: A novel coronavirus disease called COVID-19 has become pandemic in late 2019. Wuhan City was the first place detected as the source of the pandemic. Without suspicion, it spreads over the world, along with human mobility. In such a condition, every country seems quite stuttering to prepare its health system to prevent its people from the possible transmission. This paper aims to describe the preparedness of the networking laboratory in Indonesia. Methods: We conducted a rapid assessment of laboratory availability and preparedness to respond to the Covid-19 pandemic. We held the data collection on the third and fourth week of March 2020 by sending an electronic questionnaire to all 44 networking laboratories under the Ministry of Health structure. The variables assessed in this study were the availability and the requirements of the Covid-19 related laboratory's substances, including reagents and other equipment types. Results: The Ministry of Health established the networking laboratory in 2009, but due to the COVID-19 pandemic, it has renewed twice to enhance and expand the laboratory capacities over the country. Our studies showed preparedness among networking laboratories in Indonesia regarding this new emerging COVID-19 condition was quite devastating. Both surveillance and diagnostic laboratories have a similar situation. The availability of their primary materials was mediocre, but the adequacy was far beyond the capacity in handling the COVID-19 specimen. We found a similar case in the laboratory, supporting materials, and personal protective equipment (PPE). Conclusion: Laboratory preparedness during initial period of time of the COVID-19 pandemic as part of the health system is still weak. The availability of the necessary equipment, supporting materials, and personal protective equipment are far beyond the requirements. The COVID-19 has alarmed the laboratory and the whole health system in Indonesia into a new era with better future preparedness. Keywords: laboratory preparedness, COVID-19, Indonesia

Difference of hemaglutinins between wild-type and vaccine measles virus in Indonesia Made Setiawan; Agus Sjahrurachman; Fera Ibrahim; Agus Suwandono
Paediatrica Indonesiana Vol 48 No 1 (2008): January 2008
Publisher : Indonesian Pediatric Society

Background Hemaglutinin (H) protein of measles virus is veryimportant in the process of host cell infection. H protein is alsoable to induce specific antibodies which can neutralize measlesvirus and block the cell infection.Objective This study aimed to explore the nucleotide and aminoacid sequence differences between wild-type measles virus (G2,G3 and D9) with CAM-70, Schwarz and Edmonston-wt vaccinevirus.Methods The exctration and amplification of the gene wereconducted in the laboratory using biomolecular technology. Thegene and protein analysis were conducted using the bioinformatictechnology.Results The results showed that the differences in nucleotidesequences were highest between wild-type virus and CAM-70vaccine virus (76-77 nucleotides), followed by Schwarz (61-64nucleotides) and Edmonston (60-63 nucleotides). The differencesin amino acid sequences were highest between wild-type virusand CAM-70 (24-29 residues), followed by Schwarz (13-20residues) and Edmonston (12-19 residues).Conclusion The Indonesian wild-type measles virus was geneticallycloser to Schwarz vaccine virus than CAM-70 vaccine virus,hence the neutralizing antibodies generated by Schwarz vaccinewere more specific against Indonesian wild-type virus comparedto CAM-70 vaccine.

Differences of nucleotide and amino acid sequences of nucleoprotein (N) gene between wild-type measles virus and vaccine virus in Indonesia Made Setiawan; Agus Sjahrurachman; Fera Ibrahim; Agus Suwandono
Paediatrica Indonesiana Vol 48 No 2 (2008): March 2008
Publisher : Indonesian Pediatric Society

Background Measles virus is a member of genus morbiliviruswhich belongs to family paramyxovirus with negative, single-strand RNA genome. RNA is packed by nucleocapsid (N) protein.The N protein is very important for RNA replication andtranslation. Abnormality in N protein will induce abnormality invirus replication.Objective This study aimed to explore the differences ofnucleotide sequence of N gene and amino acid sequences of Nprotein between wild-type measles virus (G2, G3 and D9) andvaccine virus (CAM-70, Schwarz and Edmonston-wt)Methods The exctraction and amplification of the gene wereconducted in the laboratory using biomolecular technology. Thegene and protein analysis were conducted using the bioinformatictechnology.Results The results showed that more differences were foundbetween nucleotide sequences of N gene of wild-type measlesvirus against CAM-70 vaccine virus (77 â€“ 79 nucleotides)compared against Schwarz and Edmonston-wt vaccine virus (71-74 nucleotides). Likewise, more differences were also observedbetween amino acid sequences of N protein of wild-type measlesvirus against CAM-70 vaccine virus (18-24 residues) comparedagainst Schwarz and Edmonston-wt vaccine virus (17-23 residues).

Differences of antigenic profiles on immunoblotting of wild type measles virus and vaccine virus in Indonesia Made Setiawan; Agus Sjahrurachman; Fera lbrahim; Agus Suwandono
Paediatrica Indonesiana Vol 48 No 6 (2008): November 2008
Publisher : Indonesian Pediatric Society

Background Measles virus has a negative, single strand RNAgenome which codes for six important structural proteins. Thegenes of the wild type measles virus have many variances hencethe nucleotide sequences of each wild type virus and vaccine virusare different. This differences lead to the antigenic differencesbetween wild type and vaccine virus.Objective The purpose of this research is to investigate thedifferences in the antigenic profiles on immunoblotting betweenwild type and vaccine virus.Results The analysis results are 1) the antigen ofCAM-70 vaccinevirus was less able in cross reacting with the antibodies from G2,G3, 09, CAM-70 and Schwarz; 2) The antibody aga inst CAM-70 was only able to cross react with antigens of N protein and afew of antigens ofF proteins; 3) The wild type virus were veryimmunogenic, hence the antibody titers were very high; 4) TheCAM-70 and MMR vaccine virus were less immunogenic, hencetheir antibody were very low; 5) The antibody responses thatalways occurred from all immunized mice serum were antibodyfor N and F proteins. However, the antibody against CAM-70vaccine virus was still able to react with wild type virus (G2, G3and 09).Conclusion All antigen-antibody reaction on immunoblottingresulted in different profiles especially between wild type virusand CAM-70 vaccine virus. Although CAM-70 vaccine virusshowed clear differences compared to G2, G3 and 09 genotypes,antibodies against CAM-70 were still able to cross react withantigens from other genotypes (G2, G3 and D9).

Antigenic differences between wildtype measles viruses and vaccine viruses in Indonesia Made Setiawan; Agus Sjahrurachman; Fera Ibrahim; Agus Suwandono
Paediatrica Indonesiana Vol 48 No 3 (2008): May 2008
Publisher : Indonesian Pediatric Society

Background Measles virus has a single, negative strand RNAgenome which codes 6 structural proteins: N, F, P M, H and L.Currently there are several variances in the nucleotide sequencesof N, F, M and H genes across wild type measles viruses, hencemeasles viruses can be categorized into clades and genotypes. Theantigenicity of the previous genotype of measles is different fromthe current genotype.Objective To determine the antigenic differences between wildtype measles virus and measles vaccine virus.Methods Analysis of the antigenic differences between wild typevirus (G2, G3 and D9) and vaccine virus (CAM-70 and Schwarz)was performed by immunizing mice with the respective viruses.The serum was then tested with micro-cross-neutralizationtechnique using the G2, G3, D9 and CAM-70 virus. Tests withcross ELISA examination technique were also performed usingthe same set of virus.Results Analysis of the cross neutralization test and cross ELISAshowed that the highest antigenicity reaction was found betweenwild type virus with antibody against wild type virus, while thelowest reaction was between wild type virus with antibody againstCAM-70.Conclusions We conclude that the antigenicity of antigenic proteinfrom wild type virus is higher than antigenicity of vaccine virusprotein. In addition, it was found that the antigenicity of proteinsfrom Schwarz vaccine virus was higher than proteins CAM-70vaccine virus.

GAMBARAN FAKTOR – FAKTOR KEJADIAN LESI PRAKANKER LEHER RAHIM DI PUSKESMAS CANDIROTO, KABUPATEN TEMANGGUNG Mazroatul Khasanah; Ari Udiyono; Lintang Dian Saraswati; Agus Suwandono
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 1 (2017): JANUARI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Cervical cancer is a leading cause of mortality and morbidity after breast cancer, representing approximately 12% of all cancer in women worldwide. Early diagnostic of cervical cancer with cervical precancerous lesions. The aim of the study is to describe the incidence of precancerous cervical lesions in Puskesmas Candiroto, Temanggung Regency. The study is an observational study which uses case control approach. Population of the study are woman who has conducted VIA test in Puskesmas Candiroto in 2015 – 2016 with 120 respondents. Analysis using frequency distribution. The result of distribution shows that Offspring history has 10,0% in the control grup, education has 38,0% with elementary school, using hormonal contraception (injection) has 40% in case group and marriage under 20th has 49,2% in control group . it is suggest that women must using non hormonal contraception and keep her hygiene personal.

GAMBARAN FAKTOR RISIKO PENYAKIT PERIODONTAL PADA ANAK JALANAN DENGAN EKS ANAK JALANAN DI KOTA SEMARANG (STUDI PADA ANAK JALANAN LEPAS, ANAK PENDAMPINGAN RPSA X, DAN ANAK PGOTBALAI REHABILITASI SOSIAL Y KOTA SEMARANG) Anggit Vikasari; Agus Suwandono; Henry Setyawan Susanto
Jurnal Kesehatan Masyarakat (Undip) Vol 4, No 4 (2016): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Semarang City Health Office in 2015 found as many as 681 new cases of gum and periodontal diseases that occur in children aged 5-14 years. Periodontal disease in children by its age certainly does not recognize social status like street children or ex street children. Street children generally have poor dental hygiene degree who are influenced by maintenance of oral hygiene. The purpose of this study is to identify and describe the risk factors of periodontal disease between street children and ex street children in Semarang. This research is descriptive quantitative-qualitative method with cross-sectional design study. The results show that are proportion frequencies of risk factors in respondents. From 60 respondents are known that 23,3% of 14 years old, 51,7% students of elementary school, 71,7% have job,100% have parents with low income, 50% have good plaque status, 56,7% have moderate OHI-S status, and 40% have moderate CPITN status. Respondents are advised to brushing their teeth or using mouthwash regularly also take control for intake consumption, do not smoke, do not using drugs-psychotropic substances and drinking alcohol.

GAMBARAN PERILAKU MASYARAKAT TERHADAP RISIKO PENYAKIT PES PADA DUSUN FOKUS DAN DUSUN TERANCAM PES Cika Nirbaya Br Lubis; Agus Suwandono; Mateus Sakundarno
Jurnal Kesehatan Masyarakat (Undip) Vol 4, No 4 (2016): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Plague is a zoonotic disease caused by Yersinia pestis from rat flea bites. According to the results of trapping mice were successfully carried out in the region hamlet threatened Tlogosari and hamlets focus Taman year 2014-2015 has increased. Increasing the density of the rat is influenced by the behavior of people who are less clean and the lack of public knowledge about the plague and rats. The purpose of this study was to describe the density of rodents and social behavior at the focus hamlet and threatened hamlet. This study was a descriptive study by using quantitative methods and cross sectional study design. The population was rat gots caught up in the hamlet of focus and hamlets threatened and existing community mousetrap in his home in the hamlet of focus and Tlogosari threatened hamlets. The sample that was whole rat gets caught using live traps and all households mousetrap in that home at the hamlet of focus Taman and Tlogosari threatened hamlet with an age range 15-64 years. The results of this study showed that rats found in the hamlet focus was Rattus tanezumi while hamlet threatened were Rattus tanezumi and Rattus tiomaticus. The highest density of rats was at the hamlet threatened (6.8%). Flea infestations were found in the focus hamlet and threatened hamlet were X.cheopis and St.cognatus. Society in hamlets focus had less good behavior among other behaviors seed storage and trash management. While hamlet threatened, people who had less good behavior was the behavior of the use of personal protective equipment and trash management behavior.

Gambaran Perilaku Personal Hygiene yang Berhubungan dengan Infeksi Soil Trasmitted Helminth pada Anak Sekolah Dasar (Studi Kasus di Desa Candi Kecamatan Bandungan Kabupaten Semarang) Rozzaq Alhanif Islamudin; Agus Suwandono; Lintang Dian Saraswati; Martini Martini
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 1 (2017): JANUARI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Soil Transmitted Helminth (STH) rentan menginfeksi pada usia anak-anak. Kecacingan dapat menimbulkan gangguan pada proses pertumbuhan dan perkembangan. Tujuan dari penelitian ini untuk menganalisis gambaran perilaku personal hygiene yang berhubungan terjadinya Infeksi Soil Transmitted Helminth pada anak sekolah dasar di Desa Candi Kecamatan Bandungan Kabupaten Semarang. Pada tahun 2006 Kabupaten Semarang memiliki prevalensi infeksi kecacingan tertinggi di Provinsi Jawa Tengah yaitu 25%. Penelitian dengan pendekatan cross sectional dilakukan pada anak Sekolah Dasar sebagai subjek penelitian dan respondenya orang tua anak. Jenis penelitian yang digunakan adalah observasional analitik. Sampel penelitian sebanyak 71 anak. Teknik pengambilan sampel dilakukan secara random sampling. Untuk meningkatkan kualitas dalam pengukuran kuesionar, maka dilakukan uji validitas dan uji reliabilitas. Metode analisa data menggunakan Chi square dengan derajat kepercayaan 95%. Hasil penelitian ini menunjukan bahwa prevalensi anak terinfeksi Soil Trasmitted Helminth 11,3% dan perilaku personal hygiene baik 54,9%. Jika dilihat dari perilaku yang berhubungan dengan infeksi kecacingan yaitu, memotong kuku baik 60,6%, mencuci tangan baik 57,7%, bermain tanah baik 62,0%, pengolahan sampah baik 56,3%, pengelolaan lantai rumah baik 73,2%, pengelolaan jamban baik 69,0%, pengelolaan air bersih baik 78,9%. Kesimpulan penelitian ini perilaku personal hygiene yang berhubungan terjadinya infeksi Soil Trasmitted Helminth pada anak sekolah dasar di Desa Candi Kecamatan Bandungan Kabupaten Semarang sudah baik.

The Effectiveness of the Revitalization of “Jogo Tonggo” as Local Wisdom in Vigilance and Prevention of Transmission of COVID-19 in Central Java Province Yulianto Prabowo; Agus Suwandono; Bagoes Widjanarko; Sutopo Patriajati; Aris Sugiharto
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 3 (2022): Indian Journal of Forensic Medicine and Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i3.18315

Background: Jogo tonggo is an empowerment effort to increase community participation in preventing thetransmission and spread of Covid-19 in Central Java. The study aims to assess the effectiveness of the revitalizationof “jogo tonggo” as local wisdom toward increasing community knowledge, attitudes, and behavior towardCOVID-19.Methods: This study is true-experimental study involving 352 respondents, which are divided into the experimentalgroup and the control group. The used statistical analyses were paired simple t-test, independent t-test, Mann-Whitney, and Wilcoxon.Results: There was an increase in the mean after intervention between group 1 and group 2 including knowledgeof “jogo tonggo” (δ=2.39; p=0.001), knowledge of COVID-19 symptoms and transmission modes (δ=2.87; p=0.001),knowledge of prevention methods of COVID-19 (δ=1.63; p=0.001), attitudes towards “jogo tonggo” (δ=2.00;p=0.004), attitudes towards COVID-19 (δ=1.58; p=0.011), and COVID-19 prevention behavior (δ=6.04; p=0.001).Conclusion: The revitalization of “jogo tonggo” can increase knowledge, attitudes, and behavior toward COVID-19.

Co-Authors Aam Suryamah Adrasitta Khaliddya Fithrianni Agus Sjahrurachman Agus Suwandono Amir Su'udi Anggit Vikasari Anies Ari Udiyono Aris Sugiharto Azizah Putri Umami Bagoes Widjanarko Budi Anna Keliat Cika Nirbaya Br Lubis Deviana Yuanitasari Deviana Yuanitasari Diana Marchella Etty Mulyati Fera Ibrahim Fera lbrahim Gneissa Beltsazar Harimat Hendarwan Hazar Kusmayanti Henry Setyawan Susanto Henry Setyawan Susanto Holyness N Singadimedja Holyness N. Singadimedja Indriati Indriati Ketut Aryastami Lintang Dian Saraswati Made Dewi Susilawati Made Setiawan Martini Martini Mateus Sakundarno Mazroatul Khasanah Nelly Puspandari Nun Harrieti Rafan Darodjat Rozzaq Alhanif Islamudin Sutopo Patria Jati Syachroni Syachroni Ully Adhie Mulyani Vivi Setiawaty Yulianto Prabowo

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