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All Journal HAYATI Journal of Biosciences Medical Journal of Indonesia Indonesian Journal of Cancer Universa Medicina Jurnal Respirologi Indonesia Jurnal Ilmu Kefarmasian Indonesia Jurnal Respirasi (JR) JURNAL PENELITIAN KEPERAWATAN MEDIK Respiratory Science Cermin Dunia Kedokteran Indonesian Journal of Cancer eJournal Kedokteran Indonesia
Elisna Syahruddin
Department Of Pulmonology And Respiratory Medicine, Faculty Of Medicine, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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In vitro transcription of HIV-1 RNA for standard RNA Yasmon, Andi; Bela, Budiman; Ibrahim, Fera; Syahruddin, Elisna
Medical Journal of Indonesia Vol 20, No 3 (2011): August
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.131 KB) | DOI: 10.13181/mji.v20i3.447

Abstract

Background: The quantitative assays are important tests in the management of patients with HIV-1/AIDS. The important step in developing the assay is the availability of the standard HIV-1 RNA. For this purpose, we optimized in vitro HIV-1 RNA transcription to produce the standard HIV-1 RNA.Methods: The HIV-1 DNA was amplified from pNL43 by PCR using a primer pair that was specific for conserved region of HIV-1 Gag gene. The PCR product was further cloned into pBluescript II KS. The recombinant plasmid was restricted with EcoRI enzyme. Then, the linearized plasmid was used as template for RNA transcription. RT-PCR and PCR were performed simultaneously for confirmation of synthesized RNA fragment.Results: A 115 bp DNA of HIV-1 Gag gene has been cloned into pBluescript II SK with the exact true orientation. The reaction of the RNA transcription was also successfully performed. The RNA transcripts have been confirmed and showed the accuracy of the transcripts.Conclusion: We successfuly constructed the recombinant plasmid containing a conserved region of HIV-1 Gag gene, and the HIV-1 RNA has been transcribed in vitro as well. (Med J Indones. 2011; 20:185-9)Keywords: HIV-1/AIDS, Quantitative assay, RNA transcription
Gejala Klinis Neurologis dan Gambaran CT Scan Otak Pasien Kanker Paru Karsinoma Bukan Sel Kecil Metastasis ke Otak di Rumah Sakit Persahabatan YULIANTI, DIAN; SYAHRUDDIN, ELISNA; HUDOYO, AHMAD; ICKSAN, AZIZA
Indonesian Journal of Cancer Vol 4, No 1 (2010): Jan - Mar 2010
Publisher : "Dharmais" Cancer Center Hospital

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Abstract

Brain is one of the common sites of distant metastasis in patients of lung cancer, and incidens of brain metastasis in lung cancer is considered high. In Non-small Cell lung cancer guide line, brain CT done if the neurological symptom occured. Therefore analysis of diagnostic approach related to brain metastases is needed. Prospective analysis of 35 patients newly diagnosed cases of brain metastases in NSCLC. Cross sectional study with additional analysis to find relationship of clinical symptom to result of CT brain over periode July 2008-August 2009 in Persahabatan Hospital. Data were analysed as regards to the presence/absence of neurogical symtomps. Prognostic approach was applied to analyze survival rate.We found that 85,7% patients brain metastases with neurological symptomatic and 14,3% asymptomatic. Five (14,3%) patients with more than one neurologycal symptoms. Depend on stage and cell type of which 27 (77,1%) with adenocarcinoma, 21 (62,9%) with T4, 17 (48,6%) N0 and patients with soliter nodule 8 (22,9%). There’s no correlation between neurologycal symptom and brain metastases. Median survival time of lung cancer patients with brain metastases are 11 month. The majority lung cancer patients with brain metastasis have neurological symptoms. The most brain metatases found in patient with adenocarcinoma, T4 and N0. Median survival was increased in treated patients Keywords: Lung cancer, NSCLC, Brain metastases, Neurological symptoms
Radiology Appearance of Malignant Peripheral Nerve Sheath Tumor: a Case Report Icksan, Aziza G; Simargi, Yopi; Syahruddin, Elisna; Hidajat, Heriawaty
Indonesian Journal of Cancer Vol 3, No 4 (2009): Oct - Dec 2009
Publisher : "Dharmais" Cancer Center Hospital

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Abstract

Malignant peripheral nerve sheath tumor (MPNST) merupakan sarkoma jaringan lunak yang jarang. Tumor ini meliputi MPNST sampai 10% dari 1-2 per 100.000 populasi/tahun kasus sarkoma jaringan lunak. Makalah ini memaparkan kasus MPNST yang ditemukan di permukaan tubuh, yaitu kepala bagian frontal, temporal, parietal kanan, leher, lengan kiri, torso, dan di organ-organ dalam yaitu otak, paru-paru, hepar, ginjal kanan, intraabdomen kiri, dan tanpa terkait dengan neurofibromatosis tipe 1 (NF1) serta tanpa riwayat terapi radiasi. Pasien hanya mengeluh sakit pada benjolan di punggung. Gambaran computed tomography (CT) otak ditemukan massa jaringan lunak di subkutis frontal dan parietal, serta lesi hipodens di serebri frontal yang menyangat di perifer setelah pemberian kontras dicurigai ke arah keganasan. Pada foto toraks dan CT paru ditemukan nodul multipel. Tampak pula massa pada ginjal kanan dan massa intrabdomen kiri. Massa heterogen pada hepar lobus kiri ditemukan pada ultrasonografi abdomen. Pemeriksaan histologi memastikan diagnosis MPNST. Pasien ini direncanakan untuk perawatan paliatif.Kata kunci: Radiologi. MPNST, sarkoma
Ekspresi Protein Gen p53 yang Bermutasi pada Jaringan Sediaan Blok Parafin Kanker Paru Karsinoma Bukan Sel Kecil HANAFI, ARIF R; SYAHRUDDIN, ELISNA; HUDOYO, AHMAD
Indonesian Journal of Cancer Vol 4, No 3 (2010): Jul - Sep 2010
Publisher : "Dharmais" Cancer Center Hospital

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Abstract

The tumor supressor gen p53 mutation encodes a protein that inhibits programmed cell death (apoptosis). The protein is expressed in basal cells in normal human epithelium, but no data are available on the frequency or clinical importance of its expression in carcinoma. We studied p53 mutation protein expression in post surgery tissues of patients with non-small cell lung carcinoma (NSCLC) and evaluated the correlation between protein expressions with prognosis of diseases. We have performed a retrospective study using 43 parafin block samples of NSCLC patients who were underwent surgery in Persahabatan Hospital during 1997 to 2008. The p53 mutation protein expression were analyzed by immunohystochemical method using a monoclonal antibody specific for p53 mutation protein. The possibility that p53 mutation expression correlated with survival was investigated with the log-rank test Kaplan Meier. Patients characteristic we found male 25/43 (58.1%) female 18/43 (41.9%) with mean age 56.19 + 8.3 y.o and mostly age 40 to 60 y.o 33/43 (76.7%). Number of smoker patiens were 31/43 (72.1%). We also detected p53 mutation protein in 16/43 (37,2%) in NSCLC tissues. Regarding histopatology types were 9/18 (50%) in squamous-cell carcinomas and 7/25 (28%) in adenocarcinomas. The corellation between positive p53 mutation protein expressions with pathological staging was significant p 0,004, according to T status T1-T2 62,5% and T3-T4 23,8% have had positive p53 mutation protein. Favorable prognostic significance of p53 mutation in patients with NSCLC stage I – II, patients in the positive p53 mutataion survived longer than those in negative with respective median survival durations 28 and 18 months p 0,019. Adenocarcinomas type with p53 mutation have had median survival 11 months compared with squamose cell carcinomas 14 months.
The accuracy of Aziza’s scoring system in limited slice non-enhanced thoracic CT for the diagnosis of adult pulmonary tuberculosis Icksan, Aziza; Faisal, Arif; Syahruddin, Elisna
Medical Journal of Indonesia Vol 26, No 1 (2017): March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.852 KB) | DOI: 10.13181/mji.v26i1.1432

Abstract

Background: Tuberculosis (TB) is still a major cause of morbidity and mortality in Indonesia. Thus, a fast and accurate method in diagnosing pulmonary TB (PTB) is needed, especially in adult smear-negative-TB cases. The purpose of this study was to determine the accuracy of a novel scoring system (Aziza’s score) using clinical data and characteristic abnormalities that are found in the limited slice non-enhanced thoracic CT.Methods: This was a prospective cross-sectional study. A limited slice non-enhanced thoracic CT was performed to establish a diagnosis of PTB in patients with suspected PTB and to assess the diagnostic value of the scoring system. A reference standard used in this study was the clinical expertise of two pulmonologists. The scoring system was analyzed using bivariate and multivariate Cox regression analysis.Results: 84 of 130 suspected PTB patients were diagnosed as PTB. They were diagnosed based on pulmonary characteristic abnormalities that were found in the limited slice non-enhanced thoracic CT. The scoring system was analyzed using bivariate and multivariate Cox regression analysis. The total scores which were equal to or more than 29 could diagnose PTB with the accuracy of 96.1% (95% CIs=91.3–98.3), sensitivity of 96.5% (95% CIs=90.1–98.8), and specificity of 95.6% (95% CIs=90.1–98.8), This novel scoring method was name as an Aziza’s scoring system.Conclusion: Aziza’s scoring system performed in the limited slice non-enhanced thoracic CT has a higher accuracy compared to the reference standard for the diagnosis of adult PTB.
Masalah Kanker Paru pada Usia Lanjut Putra, Andika Chandra; Nurwidya, Fariz; Andarini, Sita; Zaini, Jamal; Syahruddin, Elisna; Hudoyo, Ahmad; Jusuf, Anwar
Cermin Dunia Kedokteran Vol 42, No 11 (2015): Kanker
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.087 KB) | DOI: 10.55175/cdk.v42i11.948

Abstract

Kanker paru masih menjadi masalah kesehatan yang sulit diatasi bukan saja karena meningkatnya jumlah perokok di Indonesia tetapi juga disebabkan tingginya mortalitas dan morbiditas penderita. Beberapa panduan pengobatan merekomendasikan penanganan multimodalitas. Tatalaksana kanker paru cenderung tidak optimal pada usia lanjut padahal hampir separuh penderita kanker paru berusia di atas 65 tahun. Pasien kanker paru berusia lanjut sering memiliki penyakit komorbid, status tampilan buruk serta risiko toksisitas terapi tinggi. Tulisan ini merupakan tinjauan pustaka tatalaksana kanker paru pada usia lanjut.Lung cancer remains a health problem in Indonesia because of increase of young smokers and its high mortality and morbidity. There is a tendency of inadequate management of lung cancer among elderly patients despite fact that almost half of lung cancer patients aged over 65 years. Lung cancer management is still challenging and need comprehensive approach since older patients are more likely to have decreased functional reserve which limit their ability to undergo surgery or receive chemotherapy. Several guidelines recommended multimodality treatment. Ageing is also associated with increased co-morbid medical conditions that could exacerbate and influence outcome. This article will discuss treatment of lung cancer in elderlies.
Validity of pleural lactate dehydrogenase measurements in assessment of pleural effusions Elisna Syahruddin; Lia G. Partakusuma; Rita Khairani
Universa Medicina Vol. 31 No. 2 (2012)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2012.v31.105-112

Abstract

Background Pleural effusions are classified into transudates and exudates based on Light’s criteria, but the main disadvantage of Light’s criteria is the misclassification of transudates as exudates in about 20% of cases. The aim of this study was to determine the validity of various biochemical parameters to differentiate pleural exudates and transudates.MethodsAn observational study to evaluate diagnostics tests was conducted at the emergency department of Persahabatan Hospital, Jakarta, from September 2010 until December 2011. In total, 119 patients with pleural effusion were evaluated. Simultaneous pleural effusion and blood samples were examined for lactate dehydrogenase (LDH), total protein, cholesterol and albumin, with the clinical diagnosis as the gold standard. ResultsThere were 104 exudative and 15 transudative pleural effusions. Light’s criteria achieved a higher overall accuracy (sensitivity 97%, specificity 80%, accuracy 95%). The optimum cut off values were pleural fluid to serum ratio of LDH 0.4 (sensitivity 95%, specificity 87%, accuracy 94%) and pleural fluid LDH of 178 IU/L (sensitivity 92%, specificity 87%, accuracy 92%). Pleural fluid cholesterol was 50 mg/dL (sensitivity 89%, specificity 53%, accuracy 85%), pleural fluid to serum cholesterol ratio 0.41 (sensitivity 75%, specificity 53%, accuracy 72%) and serum-effusion albumin gradient 1.3 g/dL (sensitivity 91%, specificity 73%, accuracy 89%). Combination of biochemical tests did not improve sensitivity or accuracy.ConclusionsLight’s criteria remain superior to other biochemical tests, but the new cut off values of LDH pleural fluid to serum ratio of 0.4 and pleural fluid LDH of 178 IU/L appears to yield a slight improvement in diagnostic accuracy.
Hypercoagulation in Lung Cancer Annisa Dian Harlivasari; Elisna Syahruddin
Jurnal Respirologi Indonesia Vol 39, No 2 (2019)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v39i2.53

Abstract

Hypercoagulation with a clinical manifestation of Venous Thromboembolism (VTE) is a frequent complication of malignancy whose incidence has increased markedly these recent years. The occurrence of VTE is likely to be triggered by multiple pathways dominated by tissue factor (TF). The VTE itself can directly lead to patient mortality and becoming the cause of death in cancer patients. Furthermore, emerging data suggest that activation of coagulation in malignancy is integrally linked with tumor biology, host associated and cancer treatment particularly with anti-angiogenesis. The clinical hypercoagulable state is linked with prognosis in patients with lung cancer, including patients receiving systemic chemotherapy. (J Respir Indo 2019; 39(2): 130-9)
Proportion of Nicotine Dependence Among High School Students Using Fagerstrom Test for Nicotine Dependence and Correlated Factors Alma Thahir Pulungan; Elisna Syahruddin; Feni Fitriani; Aria Kekalih
Jurnal Respirologi Indonesia Vol 37, No 4 (2017)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.844 KB) | DOI: 10.36497/jri.v37i4.86

Abstract

Background: The study aims to determine the difference in the proportion of nicotine dependence among high school students using Fagerstrom Test for Nicotine Dependece set in urban and rural environment. Method: Sample size is 757 high school students from six different high school consists of class 1, 2 and 3 were selected based on stratified cluster random sampling was asked to fill out the question of smoking status and filling fagerstorm test for nicotine dependence if the respondent is smokers. Result: Amount of 167 students with smoking status and nicotine dependence measured results obtained by 28 (16.8%) persons with nicotine dependence with 8 (11.1%) people in urban areas and 20 (21.1%) people in the rural area. Factors were statistically significant to nicotine dependence is gender, the originator, type of inhale, age first smoked and number of cigarettes smoked per day. CO levels of relationship with the level of nicotine dependence shows a strong and positive patterned. Conclusion: There is a significant relationship between gender, the originator, type of inhale, age first smoked, number of cigarettes smoked per day to nicotine dependence and and level of CO exhalation to nicotine dependence. (J Respir Indo. 2017; 37(4): 307-15)
Immune Checkpoint Marker of Programmed Death Ligand-1 (PD-L1) Expression in Surgical Lung Cancer Specimens Elisna Syahruddin; Jamal Zaini; Lisnawati Lisnawati; Yayi DB Susanto; Sarah Fitriani; Shanty R. Kusumawardani; Romi Baginta
Jurnal Respirologi Indonesia Vol 42, No 2 (2022)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v42i2.286

Abstract

Background: Currently immune checkpoint pathways of PD1-PD-L1 is being used to treat lung cancer and PD-L1 served as predictive biomarker. Investigations of PD-L1 expression as a target immunotherapy in lung cancer specimens in Indonesia is needed. This study aimed to evaluate PD-L1 expression in resected lung cancer specimens using immunohistochemistry techniques. Methods: Thirty surgically resected sample from lung cancer patients were obtained. The whole specimens were stained by using immunohistochemistry techniques automatically in BOND-MAX Autostainner (Leica, Germany). PD-L1 polyclonal antibody (Genetex) at 1:1500 dilution was applied to immunohistochemistry staining procedure. Clinicopathological characteristic were acquired from the hospital registry database.Result: A total of 30 surgical specimens were assessed from lung cancer patients. Twenty-four (80%) of them were Adenocarcinoma (AC), 1 (3, 33%) Adenosquamous carcinoma, 2 (6, 67%) were Squamous Cell Carcinoma (SSC), 1 (3, 33%) were Large Cell Carcinoma, 1 (3, 33%) were Neuroendocrine Carcinoma, 1 (3, 33%) were Adenoid Cyctic Carcinoma. The expression of PDL-1 positive reactivity was 16 of 30 (53.3%) specimens and we categorized into strong positivity (>50%), medium (= 50%) and low positivity (<50) from 21 specimens, 0 specimens and 7 specimens respectively.Conclusion: PD-L1 expression could be detected in lung cancer specimen using polyclonal antibody.
Co-Authors Abul A'la Al Maududi Ahmad Hudoyo Ahmad Hudoyo AHMAD HUDOYO Aldri Frinaldi Alma Thahir Pulungan Ana Rima Ana Rima Setijadi Andalucia, Rizka Andarini, Sita Andi Yasmon Andreas Infianto Anna Rozaliyani Annisa Dian Harlivasari Anwar Jusuf Anwar Jusuf Arfiansyah, Mochammad Aris Aria Kekalih Arif Faisal ARIF R HANAFI ARIF R HANAFI, ARIF R Arif Santoso Arif Santoso Ath-Thobari, Jarir AZIZA G ICKSAN AZIZA G ICKSAN, AZIZA G Aziza Icksan Aziza Icksan Budiman Bela Denny Grecius Siregar Dian Cahyadi Dian Yulianti Ermayanti, Sabrina Ermayanti, Sabrina Erni Mudhiati EVLINA SUZANNA, EVLINA Fariz, Nurwidya Feni Fitriani Fera Ibrahim Ferry Dwi Kurniawan Findra Setianingrum Fitri Nurhayati GRACE SHALMONT, GRACE Haryati Haryati Haryati Haryati Hasbullah Thabrany Heriawaty Hidajat Heriawaty Hidajat, Heriawaty Hidayat, Moulid Ida Ayu Jasminarti Infianto, Andreas Jasminarti, Ida Ayu Kurniawan, Ferry Dwi Laksmi Wulandari Lia G. Partakusuma Lisnawati Lisnawati Melva Louisa Mia Elhidsi Mulatsari, Esti Mulyati Tugiran Nathaniel Aditya Noni Novisari Soeroso Nurmadani, Labbaika PRADNYA SRI RAHAYU, PRADNYA SRI Putra, Andika Chandra Rahmat Hidayat Ramadhaniah, Fariha Rianyta Rianyta Ridhawati Sjam Rita Khairani Robiatul Adawiyah Romi Baginta Santoso, Andintia Aisyah Sarah Fitriani Sayekti, Mutia A. Setiaputra Setiaputra Setyawan, Ungky Agus Shanty R. Kusumawardani Sita Andarini Sita Andarini Sita Andarini Soehardiman, Dicky Solly Aryza Sri Melati Munir Sri Melati Munir Steven Jonathan Tamam Anugrah TAMSIL Vivian Soetikno Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Wira Winardi Yayi DB Susanto Yopi Simargi Yopi Simargi, Yopi Yuliyanti, Sayekti Yusi Anggriani Zahrah Annisa Zaini, Jamal