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Isolation of Cellulolytic Bacteria from Peat Soils as Decomposer of Oil Palm Empty Fruit Bunch . Gusmawartati; . Agustian; . Herviyanti; . Jamsari
JOURNAL OF TROPICAL SOILS Vol 22, No 1: January 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i1.47-53

Abstract

The aim of the research was to find out potential strainsof cellulolytic bacteria isolated from two tropical peat soils and to studythe potency of the isolated bacteria to decompose oil palm empty fruit bunch (EFB). The research was carried out in two stages: (1) isolation of cellulolytic bacteria from peat soils and (2) testing the potency of isolated bacteria to decompose oil palm EFB. The cellulolytic bacteria were isolated from two peat soils, i.e. a natural peat soil (forest) and a cultivated peat soil (has been used as agriculture land). Isolation of cellulolytic bacteria was conducted by preparing a series dilution of culture solutions using a streak plate method in a carboxymethyl cellulose(CMC) selective medium.Isolates that were able to form clear zones surrounding their bacterial colony were further tested to study the potency of the isolates to decompose cellulose in oil palm EFB. The cellulolytic activity of the selected isolates were further determined via production of reducing sugars in an oil palm EFB liquid medium using Nelson-Somogyi method. The results showed that there are six isolates of cellulolytic bacteria that have been identified in two tropical peat soils used in the current study. Two isolates are identified in a natural peat soil (forest) and four isolates are identified in a cultivated peat soil. The isolates collected are identified as Bacillus sp., Pseudomonassp. and Staphylococcus sp. Among the isolates, an isolate of GS II-1 produces the highest concentration of reducing sugars, namely 0.1012 unitmL-1or 101 ppm, indicating that the isolate of GS II-1 is highly potential to decompose oil palm EFB. Therefore, the isolate of GS II-1 can be used as a decomposer in the bio-conversion processes of oil palm EFB.Keywords: isolation, bacteria, cellulolytic, oil palm empty fruit bunch (EFB), peat soil
AKTIVITAS ENZIM FOSFATASE ASAM BASA PADA BEBERAPA POLA PERTANAMAN BAWANG MERAH PADA DUA KELERENGAN ALAHAN PANJANG Sri Dewi Murni; Agustian Agustian; Mimien Harianti
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 5, No 2 (2020): Jurnal Agrohita
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v5i2.2261

Abstract

Penelitian ini bertujuan untuk mengetahui aktivitas enzim fosfatase asam dan basa pada beberapa pola pertanaman bawang merah, bawang merah (monokultur), bawang merah + cabai (rotasi) dan bawang merah – bawang daun (tumpangsari). Penelitian ini dilaksanakan didaerah Alahan Panjang Sumatera Barat dari bulan Desember 2019 – Mei 2020. Sampel tanah diambil menggunakan metoda purposive random sampling pada dua kelerengan 0-8% dan 8-15% serta dua kedalaman 0-15 cm dan 15-30 cm. parameter yang dianalisis meliputi aktivitas enzim fosfatase, respirasi dan P-tersedia. Hasil penelitian menunjukkan aktivitas fosfatase asam lebih tinggi daripada aktivitas fosfatase basa, nilai fosfatase asam 0,059 - 0,065 dan fosfatase basa berkisar 0,059- 0,055. Respirasi tertinggi (62,58) terdapat pada bawang merah – bawang daun dengan kelerengan 0-8% dan terendah (49,13) pada bawang merah + cabai dengan kelerengan 0-15%. P-tersedia terendah (18,35 ppm) didapatkan pada tanaman bawang merah yang di tumpangsarikan dengan bawang daun
GULMA TITHONIA DIVERSIFOLIA INSITU PENDUKUNG PERTANIAN ORGANIK SECARA BERKELANJUTAN Yadi Jufri; Syafrimen Yasin; Agustian Agustian; Teguh Budi Prasetyo; Nurhajati Hakim
Prosiding Seminar Nasional USM Vol 2, No 1 (2019): Semnas Multidisiplin Ilmu
Publisher : Universitas Serambi Mekkah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2366.177 KB)

Abstract

Tithonia diversifolia is a weed that can be used as a source of organic material in situ to support organic agriculture in a sustainable manner. The purpose of this paper is to introduce techniques to prepare sources of in situ organic material with Tithonia diversifolia weed plants in a sustainable manner. Tithonia diversifolia weeds contain high N and K nutrients up to 4.5% and can be pruned every 2 months with a large amount of biomass that can be used as a source of organic material in the form of green fertilizer or compost with good quality and enough available throughout the growing season. The preparation technique is done in two ways, namely by cultivating aisle and cultivating a garden circle. The results showed that the provision of Tithonia diversifolia in the form of compost was able to reduce the amount of Urea and KCl fertilizer as much as 50% of its needs with corn production of 6.68 tons / ha and soybean production of 1.80 tons / ha. Tithonia diversifolia is easy to grow anywhere with poor land conditions even with a fairly good level of adaptation.
SOIL HEALTH ASSESMENT WITH CORNELL METHOD ON SOME PLANT PATTERN BY CORN AT KENAGARIAN MUNGKA Fadil Hukama Hamdi; Juniarti Juniarti; Agustian Agustian; Ika Ayu Putri Septyani
Jurnal Agrotek Tropika Vol 10, No 4 (2022): JURNAL AGROTEK TROPIKA VOL 10, NOVEMBER 2022 (ON PROGRESS)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v10i4.5961

Abstract

Planted corn by intensively made decreased soil health and decreasing soil capacity to support plant growth caused of nutrient is not available, poor microbial activity, increasing soil acidity and soil crusting. If this problem do in long term, it will make decrease soil reciliency and decrease plant producitvity. This research was aim to investigate of soil health assesment with cornell method on some plant pattern by corn at Kenagarian Mungka. This research used explorative descriptive method through land survey and soil analyses in the laboratory. The samples were taken based on purposive random sampling. There were three land unit planted by corn with plant pattern are monoculture corn, corn-eggplant and corn-cassava. Each selected soil indicator scoring to discover the soil health correlation test to know relation between parameter with soil health.This research showed that Mungka’s land unit  planted by corn have moderate-high category for soil health, monoculture corn had the highest soil health by 65.96, corn-cassava had soil health value by 63.94 and the lowest is corn-eggplant with 63.35 soil health value. This research used three component of soil those are soil chemical, biology, and physic. This research showed that some indicator have the significant determinant indicator for soil health are OM (r=0,9209), pH (r=0,9471), and respiration (r=0,6055). Farmer was do the addition of organic matter from poultry manure to the land regularly. Based on this research, organic matter is the most important thing to keep soil health especially to encourage corn productivity.
Perkembangan Temporal-Spatial Penyakit Layu Stewart (Pantoea stewartii subsp. stewartii) Pada Tanaman Jagung Temporal-Spatial Development of Stewart Wilt (Pantoea stewartii subsp. stewartii) on Corn Yulfi Desi; Trimurti Habazar; Ujang Khairul; Agustian Agustian
Jurnal Embrio Vol 10 No 1 (2018): Jurnal Embrio
Publisher : Fakultas Pertanian Universitas Tamansiswa Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.828 KB) | DOI: 10.31317/embrio.v10i1.269

Abstract

Penyakit layu Stewart merupakan penyakit penting pada tanaman jagung. Di Indonesia penyakit ini baru dilaporkan pada tahun 2008 dan pada tahun 2015 dinyatakan sebagai organisme pengganggu tumbuhan karantina (OPTK) kategori A2. Informasi tentang perkembangan dan penyebaran penyakit ini masih terbatas. Tujuan penelitian adalah: Mendapatkan model kurva perkembangan (temporal) dan pola penyebaran (spatial) penyakit layu Stewart pada tanaman jagung pada dua periode tanam. Percobaan lapangan dilaksanakan selama dua musim tanam di Nagari Koto Baru, Kecamatan Luhak Nan Duo, Kabupaten Pasaman Barat, Provinsi Sumatera Barat. Penelitian menggunakan bedengan berukuran 5,5 x 11,0 m dengan jumlah populasi 150 tanaman dengan 6 ulangan. Pengamatan meliputi insidensi penyakit (%) dan severitas penyakit (%). Model kurva perkembangan penyakit (temporal) diuji dengan model: logistic, monomolecular, dan exponential. Pola penyebaran penyakit (spatial) dianalisis dengan run dan diuji dengan uji Z: aggregate, regular, dan random. Hasil penelitian mendapatkan: Model kurva perkembangan (temporal) penyakit layu Stewart pada dua periode tanam yang berbeda adalah sama yaitu monomolecular dan Pola penyebarannya (spatial) juga sama yaitu random.
INDEKS KUALITAS TANAH PADA SATUAN LAHAN YANG DITANAMI JAGUNG DI KENAGARIAN MUNGKA, KABUPATEN LIMA PULUH KOTA Fadil Hukama Hamdi; J Juniarti; A Agustian
Jurnal Tanah dan Sumberdaya Lahan Vol. 8 No. 2 (2021)
Publisher : Departemen Tanah, Fakultas Pertanian, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (680.471 KB) | DOI: 10.21776/ub.jtsl.2021.008.2.25

Abstract

Land continuously planted with corn will decrease land quality. Good land quality is needed to support soil function as a growing medium and keep a sustainable environment. This research was aimed to identify the soil quality index at land unit planted with corn in Mungka, ,Lima Puluh Kota Regency. This research used an explorative descriptive method through land survey and soil analyses in the laboratory. The samples were taken based on purposive random sampling at each land unit under the same slope (8%). There were three land units being sampled, and those were corn-corn, corn-eggplant, and corn-cassava cropping pattern. Corn-corn ropping pattern had the best soil quality index (0.89), followed by corn-cassava (0.86), and corn-eggplant (0.85) on the top 20 cm soil depth. On the 20-40 cm soil depth, the soil quality indices 0.82, 0.82, 0.83, for corn-eggplant, corn-corn, and corn-cassava cropping pattern, respectively. The quality index of the land under the corn cropping pattern was considered good either on the top 0-20 cm or 20-40 cm soil depth. This was due to the addition of organic matter from poultry manure to the land regularly. Based on this research, it is recommended to apply OM regularly to corn cropping pattern to keep a good soil quality index.
Investigating the Effect of Using Glyphosate on Microbial Soil in Sweet Corn Cultivation Nguyen Thi Hong Tho; Agustian Agustian; Hermansah Hermansah
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 2 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.47 KB) | DOI: 10.29165/ajarcde.v7i2.270

Abstract

Glyphosate affects the activity of certain soil microbial. Depending on the soil type and concentration, Glyphosate will have different effects. The study determined the effect of Glyphosate on the microbial population and the effect of its different volumes on β-Glucosidase activity on Ultisol used in corn cultivation. The study used a completely randomized design (CRD), two factors with three replicates. The first factor was liming. The second element was Glyphosate at a dosage of 0, 5, 6, 7 L/ha. The study was conducted at the Department of Greenhouse Agriculture, Andalas University, from July to November 2022. The results showed that although there was no interaction and statistically significant between treatments for the total bacteria population. However, the treatment used lime and low volumes of Glyphosate gave the total bacteria population high density. β-Glucosidase, an enzyme produced from a specific type of bacteria in soil, decreased activity while combining lime treatment and Glyphosate, especially from the dosage at 6 L/ha, and had a statistically significant interaction between lime factor and herbicide after the second spray (p<0.05). In conclusion, Glyphosate and lime can alter and reduce microbial soil activity and number, particularly at high 6 and 7 L/ha volumes.
The Effect of Indigenous AMF Applications on The Morpho-Physiological Characteristics of Two Varieties of Shallots on Drought Stress Conditions Eka Susila; Fri Maulina; Aswaldi Anwar; Auzar Syarif; Agustian Agustian
Journal of Applied Agricultural Science and Technology Vol. 7 No. 2 (2023): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v7i2.80

Abstract

One of the limiting conditions for shallot plants to grow optimally is dry land conditions. Indigenous AMF application is one way to overcome this condition. This study aimed to determine the effect of indigenous AMF application on the morpho-physiological characters of two shallot varieties which are sensitive and tolerant to drought stress conditions. The study was carried out for 6 months on a wirehouse and laboratory scale. The Experiment used a completely randomized design with two factors. The first factor was varieties of shallots, i.e. Brebes (Sensitive) and Kuning (Tolerant) varieties of shallots. The second factor was the application of indigenous AMF which consisted of 5 levels i.e. Glomus sp1, Glomus sp2, Glomus sp3, a mixed those three isolates and control treatment (without application of AMF). The morpho-physiological observation parameters included header dry weight, root weight, and leaf proline content. From the observations, it can be concluded that under stressed conditions, the leaf proline content of the sensitive variety accumulated higher in the header than the tolerant variety, because the tolerant variety was better able to produce higher root and header weights when adapting than the sensitive. AMF inoculation did not show significant differences with the treatment without AMF inoculation on leaf proline. However, there was a tendency that inoculation of a mix of AMF isolates (Glomus sp1+Glomus sp2+ Glomus sp3) decrease the proline content in the leaves, both in sensitive and tolerant varieties so that plants are more resistant to drought stress.
DINAMIKA KARBON DAN AKTIVITAS β-GLUKOSIDASE DI TOPSOIL DAN SUBSOIL LAHAN PERTANIAN MONOKULTUR NAGARI ALAHAN PANJANG SUMATERA BARAT Sri Dewi Murni; Agustian Agustian; Mimien Harianti
Jurnal Tanah dan Sumberdaya Lahan Vol. 10 No. 2 (2023)
Publisher : Departemen Tanah, Fakultas Pertanian, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtsl.2023.010.2.22

Abstract

This study aimed to determine the dynamics of carbon and glucosidase enzyme activity in the topsoil and subsoil of monoculture land. This research was in Nagari Alahan Panjang, West Sumatra. Soil samples were taken using a purposive random sampling method on three cropping patterns: monoculture, rotation and intercropping, and two depths of 0-15 cm and 15-30 cm. The parameters analyzed included total N, organic C, exchangeable K, Ca, Mg, and Na, glucosidase, biomass C, and respiration. Data from observations were analyzed with Pearson statistics 8 correlation. The results showed that β-glucosidase activity was positively correlated with total N and organic C, biomass C was positively correlated with exchangeable Mg, and respiration was positively correlated with N total and β-glucosidase.
Investigating the Effect of Using Glyphosate on Microbial Soil in Sweet Corn Cultivation Nguyen Thi Hong Tho; Agustian Agustian; Hermansah Hermansah
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 2 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v7i2.270

Abstract

Glyphosate affects the activity of certain soil microbial. Depending on the soil type and concentration, Glyphosate will have different effects. The study determined the effect of Glyphosate on the microbial population and the effect of its different volumes on ?-Glucosidase activity on Ultisol used in corn cultivation. The study used a completely randomized design (CRD), two factors with three replicates. The first factor was liming. The second element was Glyphosate at a dosage of 0, 5, 6, 7 L/ha. The study was conducted at the Department of Greenhouse Agriculture, Andalas University, from July to November 2022. The results showed that although there was no interaction and statistically significant between treatments for the total bacteria population. However, the treatment used lime and low volumes of Glyphosate gave the total bacteria population high density. ?-Glucosidase, an enzyme produced from a specific type of bacteria in soil, decreased activity while combining lime treatment and Glyphosate, especially from the dosage at 6 L/ha, and had a statistically significant interaction between lime factor and herbicide after the second spray (p<0.05). In conclusion, Glyphosate and lime can alter and reduce microbial soil activity and number, particularly at high 6 and 7 L/ha volumes.