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Journal : ODONTO Dental Journal

THE EFFECTIVENESS OF ANCHOVY INTAKE ON EPITHELIAL SOCKET THICKNESS POST EXTRACTION Tecky Indriana; Agus Sumono; Kunti Sholihah
ODONTO : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.1.40-45

Abstract

Background: Epithelial formation or re-epithelialization is one of the parameters in the wound closure. Nutrition has become a major factors in the successful outcome during this process. Anchovy (Stolephorus sp.) contains several proteins, vitamins, and minerals that can act as supplements to support wound closure. This research proposed to understand the effect of anchovy intake toward epithelial thickness of the rats post tooth extraction.Method: This research was an experimental laboratory with a post-test-only control group design using 24 rats as a sample. Rats were divided into 2 groups, the control and the treatment group with 12 rats each. The mandibular left first molar of the samples was extracted, then given with aquadest (the control) and anchovy powder (the treatment) during 3, 5, and 7 days. All rats were decapitated after 24 hours from the last treatment, followed by tissue processing and staining with Hematoxylin-Eosin (HE) for examination. A microscope that connects with optilab on a magnification of 100x was used to measure the epithelial thickness on the thickest and thinnest part of the epithelial. The data were analysed with One-way ANOVA test and LSD test. Result: The results showed that the epithelial thickness of the treatment group was significantly increased compared to the control group (p<0.05). Conclusion: This study concludes that the anchovy (Stolephorus sp.) intake affects increasing epithelial socket thickness of the rats post tooth extraction.
Microbiological Profile in Oral Cavity Infection in Diabetic Rats with Periodontitis Ermawati, Tantin; Indriana, Tecky; Hamzah, Zahreni; Suhartini, Suhartini; Rachmawati, Dessy
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.60-71

Abstract

Background: Diabetes melitus is a chronic metabolic disease due to the inability of the pancreas to produce insulin or the body cannot use insulin effectively.Periodontitis is one of the complications of microvascular disorders that ranks second in oral cavity diseases. One of the aerobic bacteria thought to play a role in the severity of diabetes melitus is Staphylococcus aureus, while the pathogenic anaerobic bacteria in periodontal disease are Treponema, Bacteroides, Porphyromonas, Prevotella, Capnocytophaga, Peptostreptococcus, Fusobacterium, Actinobacillus, and Eikenella. The study aimed to determine the microbiological profile in oral cavity infections of rats with diabetes melitus and periodontitisMethod: This study was experimental laboratories on male wistar rats induced by diabetes melitus and periodontitis using wire ligature on mandibular molars, which were divided into 3 treatment groups: group I (control), group II (diabetes melitus) and group III (diabetes melitus and periodontitis). Periodontitis (wire) rats were treated for 7 days and saliva was collected to identify the microbiological profile of the oral cavity. Result: Identification indicated the presence of genus and species of bacteria in the saliva of rats in 3 groups, gram positive: Staphylococcus intermedius, Staphylococcus cohnii subsp.urelyticus. Gram negative: Chromobacterium violaceum, Kleibseilla pneumoniae, Eikenella corrodens, Enterobacter sakazaki and Chryseobacterium meningosepticum. Conclusion: The number of bacterial colonies in the treatment group of diabetes melitus and periodontitis rats was greater than the treatment group of diabetes melitus rats and the control group. Gram staining results found groups of gram positive and gram negative bacteria.Â