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THE EFFECTIVENESS OF PHALERIA MACROCARPA’S LEAF NANOEMULSION GEL ON STAPHYLOCOCCUS AUREUS BIOFILM THICKNESS (IN VITRO) Rosa Pratiwi; Feny Nursyaputri; Recita Indraswary; Irma Dewi Ratnawati
ODONTO : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.0.69-79

Background: Periodontal disease often occurs in the oral cavity with 75.6-78.3% at the age of 35-44 years. One of the causes of periodontal disease is the accumulation of Staphylococcus aureus biofilm in the early colonization of the formation of the dental pellicle. Phaleria macrocarpa, also known as Gods Crown, is a traditional plant with antibacterial properties that can be used in the health sector. Nanoemulsion gel technology has the advantage of increasing the stability of the material. Aim the study to determine the ratio of the effectiveness of Phaleria macrocarpa’s leaf nanoemulsion gel 10%, 20%, 30% to decrease Staphylococcus aureus biofilm thickness.Method: This research method was in vitro experimental laboratory research using a post-test control design. Thirty samples were divided into five groups: Phaleria macrocarpa’s leaf nanoemulsion gel 10%, 20%, 30%, positive control using chlorhexidine gluconate 0.2% and negative control using aqua dest. Samples were incubated for 4 and 8 hours. Optical density readings were carried out to see the biofilm thickness after being given a Gods Crown leaves nanoemulsion gel Results: The mean of Phaleria macrocarpa’s leaf nanoemulsion gel, 30%, produced the lowest average optical density value. The incubation time of 4 hours resulted in a lower optical density value than 8 hours. The Kruskal-Wallis was p>0.05 showed there was no difference in biofilm thickness in each group.Conclusion: the effectiveness of the nanoemulsion group of Dewa crown leaf gel with a concentration of 30% was better than the 0.2% chlorhexidine gluconate gel and sterile distilled water group.

ANTIBACTERIAL EFFECTS OF BLACK QUICK (Nigella sativa L.) OIL EMULGEL AGAINST Streptococcus mutans (IN VITRO) Irma Dewi Ratnawati; Linda Septiana; Alrevo Panji Auradewa
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.113-122

Background: Secondary infection of traumatic ulcer can be caused by Streptococcus mutans. Black seed oil emulgel (Nigella sativa L.) has a saponin that has antibacterial effect on Streptococcus mutans growth. The purpose of this research for know the antibacterial effect of black seed oil emulgel (Nigella sativa L.) on Streptococcus mutans growth in vitro.Method: Experimental laboratory in vitro was held using post-test only control group design. There are four groups: negative control group and black seed oil emulgel in 5%, 7%, and 10%. Disk diffusion method was used in this research. Data analyzed by Anova test.Result: The result indicates some significant effect of black seed oil emulgel on Streptococcus mutans growth from Anova test 0,000(<0,05).Conclusion: It can be concluded that there is antibacterial effect on black seed oil emulgel to Streptococcus mutans growth.

THE EFFECTIVENESS OF PHALERIA MACROCARPA’S LEAF NANOEMULSION GEL ON STAPHYLOCOCCUS AUREUS BIOFILM THICKNESS (IN VITRO) Rosa Pratiwi; Feny Nursyaputri; Recita Indraswary; Irma Dewi Ratnawati
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Periodontal disease often occurs in the oral cavity with 75.6-78.3% at the age of 35-44 years. One of the causes of periodontal disease is the accumulation of Staphylococcus aureus biofilm in the early colonization of the formation of the dental pellicle. Phaleria macrocarpa, also known as Gods Crown, is a traditional plant with antibacterial properties that can be used in the health sector. Nanoemulsion gel technology has the advantage of increasing the stability of the material. Aim the study to determine the ratio of the effectiveness of Phaleria macrocarpa’s leaf nanoemulsion gel 10%, 20%, 30% to decrease Staphylococcus aureus biofilm thickness.Method: This research method was in vitro experimental laboratory research using a post-test control design. Thirty samples were divided into five groups: Phaleria macrocarpa’s leaf nanoemulsion gel 10%, 20%, 30%, positive control using chlorhexidine gluconate 0.2% and negative control using aqua dest. Samples were incubated for 4 and 8 hours. Optical density readings were carried out to see the biofilm thickness after being given a Gods Crown leaves nanoemulsion gel Results: The mean of Phaleria macrocarpa’s leaf nanoemulsion gel, 30%, produced the lowest average optical density value. The incubation time of 4 hours resulted in a lower optical density value than 8 hours. The Kruskal-Wallis was p>0.05 showed there was no difference in biofilm thickness in each group.Conclusion: the effectiveness of the nanoemulsion group of Dewa crown leaf gel with a concentration of 30% was better than the 0.2% chlorhexidine gluconate gel and sterile distilled water group.

EFFECTIVENESS OF BELUNTAS EXTRACT GEL (Pluchea indica) ON THE GROWTH OF Porphyromonas gingivalis Irma Dewi Ratnawati; Lulu Sa’adah; Budi Suhartono
Jurnal Medali Vol 5, No 2 (2023): Media Dental Intelektual September 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.5.2.125-131

Background: Periodontal disease is common in Indonesia, especially periodontitis, with a prevalence of 74.1%. One of the main etiologies of periodontitis is Porphyromonas gingivalis which colonizes dental plaque in the stages of secondary colonization and maturation. Pluchea indica leaves contain active substances with antibacterial properties, including flavonoids, tannins, alkaloids, saponin, and atsiri oil which can be used as additional pharmacotherapeutic therapy in the treatment of periodontitis. The gel preparation has good dispersion, practical, easy to apply. Aim the study to specify the effectiveness of Pluchea indica extract gel 40%, 60% and 80% against the growth of Porphyromonas gingivalis. Method: This research employed an in vitro experimental post-test control design. Thirty samples were split into five groups: the 40%, 60%, and 80% concentrations of the Pluchea indica leaves gel, positive control using metronidazole gel, and negative control. Samples underwent 48 hours of incubation. After being administered a Pluchea indica leaves gel, the diffusion method is used to determine the sensitivity of the test bacteria to antibacterial. Result: The diameter of the inhibition zone formed by Pluchea indica extract gel group with a concentration of around 80% was in the category very strong. Based on the results of the Tukey post-hoc test p

The Patient Satisfaction Level Analysis on the Application of Compliance with Islamic Law Elements in Sultan Agung Islamic Oral and Dental Hospital Moh Husnun Niam; Daffa Aulia Syaharani; Irma Dewi Ratnawati
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.276-283

Background: The existence of hospitals that apply sharia hospital standards can be a solution to muslim communities in Indonesia. Many hospitals currently go by the label of being Islamic hospitals, but their applications have yet to comply with sharia standards. The success of hospital services can be seen in service quality, one can be seen from element of compliance with Islamic law.Method: This study applied analytical observational research (cross-sectional) using an accidental sampling technique of 133 patients. The variables in this study included the independent variable, the application of the element of compliance with Islamic law, while the level of patients' satisfaction served as its dependent variable. Distribution of a questionnaire was used for the instrument. Analysis of the data that has been obtained is then processed using the SPSS Spearman correlation test program. Result: This study used a program using the Spearman correlation test. The study showed a relationship between applying the element of compliance with Islamic law and the level of patients' satisfaction at Rumah Sakit Islam Gigi dan Mulut Pendidikan (RSIGMP) Sultan Agung Semarang (P=0,000). Applying the element of compliance with Islamic law revealed that results averaged 79,9 fell into the range 61-90 being very good and patients' satisfaction yielded an average of 22,5 fell into the range 16,7-25 being very satisfied.Conclusion: The level of patients' satisfaction is related to applying the element of compliance with Islamic law. The better the application of the element of compliance with Islamic law, the better the patients' satisfaction. Applying the compliance with Islamic law and patients’ satisfaction have been performed properly.

Root coverage using modified tunneling technique with acellular dermal matrix for treatment of gingival recession associated with orthodontic treatment: A case report Rosa Pratiwi; Irma Dewi Ratnawati; Agung Krismariono
Odonto : Dental Journal Vol 10 (2023): Special Issue 1. October 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.0.47-53

Background: Gingival recession has a number of causes, including orthodontic therapy. The degree and extent of gingival recession and orthodontic therapy are strongly correlated. Care must be taken in choosing the best soft tissue grafting technique. Development of a number of new and improved surgical techniques, including the modified tunneling technique. Case Management: A-24-year-old female patient came to Periodontic Clinic Dental Hospital of Airlangga University with a chief complain of gingival recession in mandibular central incisors since 6 months ago. The patient complained about dental sensitivity. The dental history revealed that she had undergone fixed orthodontic therapy for 7 years. She revealed the fixed orthodontic appliance was put off since 8 months ago. The patient concern about her appearance and aesthetic problem. Gingival recession on buccal surface tooth 31 and 41 extending 1,5-2 mm apical of the incisors was found in clinical assessment. This case classified as Miller class III. The doctor planned root coverage procedure using modified tunnel technique. Acellular dermal matrix (SuredermÂ®) was chosen as a gingival graft. Discussion: Creating a "tunnel" through the buccal mucosa to treat gingival recession enables coronal repositioning of the soft tissue with predictable root coverage and a pleasing appearance. Tissue graft implantation is made possible by a horizontal incision. Conclusion: In brief, root coverage using modified tunnel technique is effective and predictable treatment modality. Comparing with other root coverage method it give quite satisfying result. The use of Acellular Dermal Matrix (ADM) considered as an alternative to Connective Tissue Graft (CTG).

Conventional gingivectomy for chronic gingival enlargement in orthodontic treatment: a case report Irma Dewi Ratnawati; Rosa Pratiwi
Odonto : Dental Journal Vol 10 (2023): Special Issue 1. October 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.0.69-75

Background: Gingival enlargement, also known as gingival overgrowth, is an enlargement of the gum tissue. There are many possible causes for enlarged gums. Gingival enlargement is general feature of gingival diseases. It is common note to chronic inflammatory gingival enlargement was caused by poor of oral hygiene. Orthodontic braces can interfere with good oral hygiene, contributing to the development of the inflammatory process. One of the most common soft tissue problems related to fixed orthodontic appliances is gingival hypertrophy or hyperplasia. Maintaining oral hygiene is hindered in cases of gingival hypertrophy due to orthodontic appliances. The most widely used surgical method to treat gingival hypertrophy is gingivectomy. Case Report : A 27-year-old patient was referred to the periodontal clinic of Airlangga University Dental Hospital because of primary gingival swelling with gingival bleeding during brushing in the anterior mandibular region. On intra oral examination showed excessive gingival with probing depth was range 4 mm on teeth 42-32, revealing the presence of pseudopockets. Clinical examination revealed generalized gingival enlargement as well as generalized bleeding on examination. After explaining the condition and treatment to the patient, he was registered for phase I treatment. After evaluating phase I therapies, phase II treatment with gingivectomy and gingivoplasty were required using a scalpel. Conclusion: Conventional gingivectomy gives satisfactory result in treating the inflammatory gingival enlargement.

Antibacterial Effectivity of Nanoemulgel Phaleria macrocarpa’s leaf on the Porphyromonas gingivalis ATCC 33277 Biofilm Thickness Rosa Pratiwi; Irma Dewi Ratnawati; Ade Ismail; Arlina Nurhapsari; Rosalita Amelia
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.168-174

Porphyromonas gingivalis is an oppoturnistic microorganism in periodontal disease that is formed at the stage maturation of biofilm formation. One of the natural materials biofilm reduction is the Phaleria macrocarpa. Phaleria macrocarpa’s leaf contains chemical compounds such as flavonoids, tannins, alkaloids, saponins that function as antibacterials.The aim of this research is to determine the effectiveness of the nanoemulsion of the gel Phaleria macrocarpa’s leaf on the thickness of the P. gingivalis bacterial biofilm. The research design was carried out with a post test only with a total of 28 samples, then divided into 4 groups of treatment of Phaleria macrocarpa’s leaf extract nanoemulsion gel preparations with variations in concentrations of 30%, 40%, and 50%, as well as a control group of 25% metronidazole gel. The reading of the anti-biofilm thickness test results was measured by OD (Optical density) using an ELISA-reader. One way Anova parametric test among obtained p<0.000 results showing the effect of Phaleria macrocarpa’ leaf gel nanoemulsion extract on the decrease in the thickness of the Porphyromonas gingivalis bacterial biofilm. It can be concluded that the nanoemulsion gel formulation of Phaleria macrocarpa’s leaf extract concentrations of 30%, 40%, and 50% is effective, with a concentration 50% was most effective against reducing the thickness of the bacterial biofilm Porphyromonas gingivalis.

The Patient Satisfaction Level Analysis on the Application of Compliance with Islamic Law Elements in Sultan Agung Islamic Oral and Dental Hospital Moh Husnun Niam; Daffa Aulia Syaharani; Irma Dewi Ratnawati
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.276-283

Background: The existence of hospitals that apply sharia hospital standards can be a solution to muslim communities in Indonesia. Many hospitals currently go by the label of being Islamic hospitals, but their applications have yet to comply with sharia standards. The success of hospital services can be seen in service quality, one can be seen from element of compliance with Islamic law.Method: This study applied analytical observational research (cross-sectional) using an accidental sampling technique of 133 patients. The variables in this study included the independent variable, the application of the element of compliance with Islamic law, while the level of patients' satisfaction served as its dependent variable. Distribution of a questionnaire was used for the instrument. Analysis of the data that has been obtained is then processed using the SPSS Spearman correlation test program. Result: This study used a program using the Spearman correlation test. The study showed a relationship between applying the element of compliance with Islamic law and the level of patients' satisfaction at Rumah Sakit Islam Gigi dan Mulut Pendidikan (RSIGMP) Sultan Agung Semarang (P=0,000). Applying the element of compliance with Islamic law revealed that results averaged 79,9 fell into the range 61-90 being very good and patients' satisfaction yielded an average of 22,5 fell into the range 16,7-25 being very satisfied.Conclusion: The level of patients' satisfaction is related to applying the element of compliance with Islamic law. The better the application of the element of compliance with Islamic law, the better the patients' satisfaction. Applying the compliance with Islamic law and patients’ satisfaction have been performed properly.

Antibacterial Effectivity of Nanoemulgel Phaleria macrocarpa’s leaf on the Porphyromonas gingivalis ATCC 33277 Biofilm Thickness Rosa Pratiwi; Irma Dewi Ratnawati; Ade Ismail; Arlina Nurhapsari; Rosalita Amelia
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.168-174

Porphyromonas gingivalis is an oppoturnistic microorganism in periodontal disease that is formed at the stage maturation of biofilm formation. One of the natural materials biofilm reduction is the Phaleria macrocarpa. Phaleria macrocarpa’s leaf contains chemical compounds such as flavonoids, tannins, alkaloids, saponins that function as antibacterials.The aim of this research is to determine the effectiveness of the nanoemulsion of the gel Phaleria macrocarpa’s leaf on the thickness of the P. gingivalis bacterial biofilm. The research design was carried out with a post test only with a total of 28 samples, then divided into 4 groups of treatment of Phaleria macrocarpa’s leaf extract nanoemulsion gel preparations with variations in concentrations of 30%, 40%, and 50%, as well as a control group of 25% metronidazole gel. The reading of the anti-biofilm thickness test results was measured by OD (Optical density) using an ELISA-reader. One way Anova parametric test among obtained p<0.000 results showing the effect of Phaleria macrocarpa’ leaf gel nanoemulsion extract on the decrease in the thickness of the Porphyromonas gingivalis bacterial biofilm. It can be concluded that the nanoemulsion gel formulation of Phaleria macrocarpa’s leaf extract concentrations of 30%, 40%, and 50% is effective, with a concentration 50% was most effective against reducing the thickness of the bacterial biofilm Porphyromonas gingivalis.

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