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All Journal JURNAL HUTAN LESTARI Jurnal Intelektualita: Keislaman, Sosial, dan Sains Al Ilmi Biota Bio Ilmi: Jurnal Pendidikan Jurnal SainHealth Jurnal Biosilampari: Jurnal Biologi INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Bioscientist : Jurnal Ilmiah Biologi
Syarifah Syarifah
Universitas Islam Negeri Raden Fatah Palembang
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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The Potential of Moringa oleifera Extract Waste as Fe Adsorbent in South Sumatra, Indonesia Syarifah Syarifah; Ra Hoetari Tirta Amelia; Riri Novita Sunarti; Awalul Fatiqin; Yahya Febrianto; Thathit Suprayogi; Yithro Serang
Biota Vol 9 No 2 (2023): Jurnal Biota 2023
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v9i2.16664

Abstract

The issue of environmental pollution has become a concern, especially for the government, since the establishment of Law No. 4 of 1982. This is because the effects of pollution can be toxic and even fatal for living beings, particularly humans. One of the pollutants in the environment generated from mining industry waste is heavy metal Fe. In light of these problems, a study was conducted to investigate the effectiveness of sawdust and Moringa twigs as by-products in adsorbing heavy metal iron (Fe) in the liquid waste of the gold mining industry. This research is classified as pure experimental research. The research reveals that the mean percentage reduction in the heavy metal iron (Fe) content in the gold mining industry wastewater, after treatment with the addition of Moringa leaf powder, is 9.6%. On the other hand, treatment with the addition of Moringa stem powder achieves a reduction of 92%. This significant difference is attributed to the bioactive compound rhamnosyloxy-benzyl isothiocyanate present in Moringa. This compound has the ability to adsorb and neutralize sludge and metal particles found in the waste suspension, along with dirt particles in the water. Consequently, Moringa shows potential as a natural coagulant for water purification purposes.
Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 Siti Soleha; Syarifah Syarifah; Tito Nurseha; Awalul Fatiqin; Endah Retnaningrum; Yitro Serang
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and Valuable Research Towards Diagnosis, Prognosis and Treatment of Dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5596

Abstract

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization of the Moraxella sp. SBE01 lipase coding gene is necessary for large-scale lipase production through genetic engineering. This study aimed to observe the molecular weight, amino acid sequence, length, and conserved amino acids in the DNA encoding the lipase gene, with the goal of identifying and characterizing the lipase-coding gene from Moraxella sp. SBE01. The primer design process was conducted to amplify the lipase gene from Moraxella sp. SBE01 using specialized software for sequence alignment and phylogenetic analysis. Amplification was carried out using PCR with the designed primer, forward primer (GTC ATG ATG TAC TTC CAY GGN GGN GG), reverse primer (GGT TGC CGC CGG CDS WRT CNC C). PCR was carried out under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles of denaturation at 95°C, annealing at 66°C (30 seconds), 70°C elongations (1 minute) and final elongation of 70°C (10 minutes). The PCR results were electrophoresed using 1% agarose gel with a 1 kb DNA marker. The PCR results were sequenced and analyzed for gene and amino acid sequences and the type of lipase expressed. Sequencing resulted in 387 bp of the nucleotide sequence. The gene and amino acid sequences from Moraxella sp. SBE01 had high homology with the gene and amino acid sequences from Moraxella sp. strain TA144. The lipase gene encodes a protein consisting of 129 amino acids and contains a conserved HGG (His-Gly-Gly) motif, which is characteristic of lipases in family IV, also known as the hormone-sensitive lipase (HSL) family. This conserved sequence suggests that the lipase shares structural and functional similarities with other enzymes in the HSL family, playing a key role in lipid metabolism.
Co-Authors ., Marleni ., Munir Abdurrahmansyah Abdurrahmansyah Adida Igandi Afriansyah, Dini Agustiani Dumeva al Hidayat Amin Nurokhman Anak Agung Istri Sri Wiadnyani Anggun Wicaksono Choirun Niswah Elvira Destiansari Emi Roslinda Endah Retnaningrum Fahmy Armanda Fatiqin, Awalul Firdaus firdaus Fitri Astriawati Fitria Sany Hoetary Tirta Amallia Imamul Huda Irham Falahudin Istiroha Istiroha Kurratul aini Meysi Rahmalia Meysi Rahmalia Mona Desti Ersa Muhammad Hisyam Ihsan Nur Fitri Aisyah Puput Maryati Ra Hoetari Tirta Amelia Rahma Darnella Rian Oktiansyah Riri Novita Sunarti Siti Soleha Syahidah Fitriah Thathit Suprayogi Tiara Dwi Anjani Tito Nurseha Tuti Handayani Ummi Hiras Habisukan Vivi Weliyani Weni Lestari Wilia Apriani Wiwik Ekyastuti Yahya Febrianto Yithro Serang Yitro Serang Yulia Tri Samiha Yustina Hapida Yustina Hapida, Yustina Zainal Berlian zainal burlian