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Developing PBL E-Learning with the Brainwriting Method to Improve HOTS and Learning Interest in the Circulatory System Aulya, Rahmi; Ariyanti, Nur Aeni
Jurnal Pendidikan Matematika dan Sains Vol. 14 No. 1 (2026): February 2026
Publisher : Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jpms.v14i1.92926

Abstract

This study aims to determine the feasibility, practicality, and effectiveness of PBL e-learning using the brainwriting method to enhance students’ Higher-Order Thinking Skills (HOTS) and learning interest. This research employs a research and development approach using the ADDIE  model. The research instruments included media validation sheets from material and media experts, questionnaires, observation sheets, and test questions. The research results are as follows: (1) PBL e-learning with the brainwriting method is feasible for enhancing students’ HOTS and learning interest; (2) this e-learning is practical for use in learning activities, as indicated by assessments from students and teachers, with a practicality score of 98, categorized as “very practical”; and (3) this e-learning is effective in improving students’ HOTS and learning interest, as shown by the MANOVA test results, with a significance value of 0.000 (< 0.05). These findings suggest that integrating Problem-Based Learning (PBL) with the brainwriting method in an e-learning environment can be an effective instructional strategy to foster higher-order thinking skills and increase students’ engagement in biology learning. Furthermore, this study contributes to the achievement of the Sustainable Development Goals, particularly SDG 4 (Quality Education), by promoting inclusive, innovative, and technology-enhanced learning practices that support the development of critical thinking skills essential for lifelong learning in the 21st century.
Characterization and Identification of Indol Acetic Acid Phytohormone-producing Bacteria from Corn Root (Zea mays L.) Putri Maharani, Rendha; Aeni Ariyanti, Nur; Aloysius, Suyitno; Sugiyarto, Lili
Jurnal Sains Dasar Vol. 14 No. 2 (2025): October 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

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Abstract

Rhizobacteria are endophytic bacteria that inhabit plant tissues and the rhizosphere, producing secondary metabolites that support plant growth. This study is an exploratory descriptive investigation aimed at characterizing and identifying IAA- and phytohormone-producing endophytic rhizobacteria isolated from the roots of corn plants (Zea mays L.). The rhizobacteria isolates producing IAA phytohormones were obtained by culturing bacteria in Yeast Peptone Broth (YPB) medium supplemented with L-tryptophan. Bacterial cultures (1 mL) were treated with Salkowski reagent, and absorbance was measured spectrophotometrically at a wavelength of 530 nm. From 14 isolates, five were identified as producing the highest levels of indole-3-acetic acid (IAA), ranging from 17 to 50 ppm. Identification was conducted using the profile matching method to determine the genus of each rhizobacterium by comparing its characteristics with reference genera. Based on 62 tested traits, the five isolates exhibited diverse features, including Gram-positive and Gram-negative bacteria, coccus-shaped morphology, circular and irregular colony configurations, lobate and undulate margins, convex, umbonate, and raised elevations, and colony colors ranging from white to yellowish, along with varied physiological (biochemical) test results. The identification results revealed bacteria with a similarity index of ≥70%, suggesting proximity to the genus Micrococcus (77.4%, isolate N), Rhizobium (80.6%, isolate D), and Shewanella (80.6%, isolate B; 87.1%, isolate V; and 91.9%, isolate E). This research provides an important contribution to the development of biofertilizers aimed at enhancing the growth and productivity of corn plants sustainably.