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Journal : Vitek : Bidang Kedokteran Hewan

Gambaran histopatologi radang ekstrak daun jintan (coleus ambonicus) terhadap induksi uric acid dan oxonic acid pada tikus putih wistar (rattus norvegicus) Rondius Solfaine
VITEK : Bidang Kedokteran Hewan Vol 4 (2014): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

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Abstract

Tujuan penelitian ini adalah menganalisis daya anti radang ekstrak daun jintan (coleus amboinicus) yang diinduksi nephropathy akut. Ekstrak daun jintan diperoleh dari daun jintan (Coleus amboinicus) segar yang diekstraksi dengan metode maserasi etanol 96 % dan dianalisis secara kualitatif dengan metode kromatografi lapis tipis (KLT) untuk mengidentifikasi senyawa aktif yang terkandung di dalamnya. Aktivitas ekstrak daun jintan sebagai anti radang dilakukan dengan pemberian secara intraperitoneal ekstrak daun jintan (Coleus amboinicus) pada tikus putih galur Wistar terhadap induksi granuloma fibrosis pada tubulus ginjal yang diinduksi dengan senyawa Oxonic Acid dan Uric Acid (OA & UA). Pembentukan granuloma fibrosis diidentifikasi sebagai nephropahty akut dengan teknik sediaan histopatologi pengecatan Haematoxilin & Eosin (HE). Hasil penelitian menunjukkan bahwa ekstrak daun Jintan (coleus ambonicus) mempunyai kandungan kualitatif kumarin, saponin, minyak atsiri dan polifenol dengan teknik Kromatografi lapis Tipis (KLT). Induksi intra peritoneal Oxonic Acid dan Uric Acid menyebabkan perubahan makroskopis berupa deposisi kristal urea intraperitoneal dan keradangan pada sendi metatarsal. Secara histopatologis induksi OA & UA menimbulkan nephropathy akut yang ditandai dengan adanya lesi degenerasi, keradangan dan nekrosis pada tubulus dan glomerulus ginjal pada kelompok perlakuan. Pemberian ekstrak daun Jintan pada kelompok perlakuan menghambat terjadinya pembentukan nephropathy akut secara nyata (P<0.05) dibandingkan kontrol dan kelompok induksi.
Kasus hipokalsemia pada sapi perah FH dI KUD Tani Wilis Sendang Tulung Agung Rondius Solfaine; Bayu Adi Lestari
VITEK : Bidang Kedokteran Hewan Vol 4 (2014): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

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Abstract

Hipokalsemia merupakan suatu gangguan metabolisme pada sapi perah dapat terjadi sebelum, sewaktu, atau beberapa jam sampai dengan 72 jam setelah melahirkan. Kejadian ini ditandai dengan penurunan yang tiba-tiba kadar calcium darah dari jumlah normal 9 - 10 mg/dl menjadi 3 - 7 mg/dl. Penelitian ini bertujuan untuk mengetahui tentang jumlah kasus, gejala klinis, dan penanganan penyakit hipokalsemia pada sapi perah di Koperasi Unit Desa Tani Wilis Sendang Tulungagung pada tahun 2012.
Efektifitas ekstrak daun kembang bulan (Tithonia diversifolia) terhadap gambaran histopatologi pankreas pada tikus yang diinduksi aloksan Roeswandono Wirjatmadja; Rondius Solfaine; Dian Ayu Kartika Sari; Anisa Novia Wati
VITEK : Bidang Kedokteran Hewan Vol 11 No 1 (2021): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v11i1.52

Abstract

This study was conducted to determine the histopathological picture of the pancreas by induction of alloxan and extracts of the moon flower (tithonia diversifolia) in wistar rats (rattus norvegicus). A total of 24 male Wistar rats with 4 treatments were control P0, alloxan P1 with 1% CMC Na, P2 alloxan with 100mg / kgBB extract, alloxan P3 with 200mg / kgBB extract. Mice after said diabetes mellitus with a number> 135 mg / dL were then given treatment with 1% CMC Na on P1 and lunar leaf extract at P2 and P3, necropsy was carried out on the 15th day and the pancreatic organs were taken to be made in the form of preparations with coloring. HE, lesions observed Degeneration, Inflammatory Cells and Necrosis using a microscope, the results were analyzed using Kruskal wallis followed by Mann withney. The data obtained by degeneration in the kruskal wallis test were very significantly different because there were significant differences (P = 0.01), inflammation cells in the Kruskal wallis test were very significantly different because there were significant differences (P = 0.00), and for necrosis in the Kruskal wallis test significantly different because there are significant differences (0.02).
GAMBARAN HISTOPATOLOGI PARU PADA MENCIT (Mus musculus) YANG DIPAPAR ASAP ROKOK KRETEK AKIBAT PENGARUH PEMBERIAN INFUSA TEH HIJAU (Camellia sinensis) Dyah Widhowati; Rondius Solfaine; Olan Rahayu Puji Astuti Mussa; Apriska Ayuningtias
VITEK : Bidang Kedokteran Hewan Vol 10 (2020): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v10i0.53

Abstract

This study was conducted to determine the effect of antioxidant administration from green tea infusion (Camellia sinensis) on damage to the histological structure of the lung exposed to clove cigarette smoke in mice (Mus muscullus). Free radicals in cigarette smoke such as tar, nicotine, and carbon monoxide can cause various damages to the lung or This study was conducted to determine the effect of antioxidant administration from green tea infusion (Camellia sinensis) on damage to the histological structure of the lung exposed to clove cigarette smoke in mice (Mus muscullus). Free radicals in cigarette smoke such as tar, nicotine, and carbon monoxide can cause various damages to the lung organs due to oxidative stress caused by the reaction of ultrafine particles with cells. A total of 27 mice were taken from the Biochemical Laboratory of Airlangga University, Surabaya. In this study mice (Mus muscullus) were exposed to kretek cigarette smoke in the smoking chamber for 15 minutes in a period of 2 weeks, used 2 variants of antioxidant doses of green tea infusion of 0.45 gr to 0.6 gr. After the treatment was completed, the preparation of the mice's pulmonary organ preparations and damage to the structure of the lung organ were observed in 9 visual fields. The results showed that there was a relationship between the amount of cigarette smoke exposed and damage to the lung organs of mice when given antioxidant green tea. The administration of green tea antioxidants reduced inflammation of the lung organ infiltration of inflammatory cells by 44%, edema by 55%, and destruction of septa alveoli by 88%. gans due to oxidative stress caused by the reaction of ultrafine particles with cells. A total of 27 mice were taken from the Biochemical Laboratory of Airlangga University, Surabaya. In this study mice (Mus muscullus) were exposed to kretek cigarette smoke in the smoking chamber for 15 minutes in a period of 2 weeks, used 2 variants of antioxidant doses of green tea infusion of 0.45 gr to 0.6 gr. After the treatment was completed, the preparation of the mice's pulmonary organ preparations and damage to the structure of the lung organ were observed in 9 visual fields. The results showed that there was a relationship between the amount of cigarette smoke exposed and damage to the lung organs of mice when given antioxidant green tea. The administration of green tea antioxidants reduced inflammation of the lung organ infiltration of inflammatory cells by 44%, edema by 55%, and destruction of septa alveoli by 88%.
PERBANDINGAN PAPARAN ASAP ROKOK KONVENSIONAL DAN ROKOK HERBAL PADA MENCIT (Mus musculus) TERHADAP PERBANDINGAN GAMBARAN HISTOLOGI PARU Nurul Hidayah; Olan Rahayu Puji Astuti Mussa; Rondius Solfaine; Yudha Sapto Utomo
VITEK : Bidang Kedokteran Hewan Vol 10 (2020): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v10i0.55

Abstract

The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p> 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p <0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p <0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes. The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p> 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p <0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p <0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes.
Perbandingan pemberian minyak kuning telur ayam dan bioplacenton terhadap gambaran histologi kulit Ratna Widyawati; Rondius Solfaine; Dian Ayu Kartika Sari; Yoko Shagita
VITEK : Bidang Kedokteran Hewan Vol 11 No 1 (2021): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v11i1.70

Abstract

This study was conducted to determine the comparison of re-epithelialization, granulation and the number of inflammatory cells infiltration between topical application of chicken yolk oil and bioplacenton on white rat (Rattus norvegicus) skin that has induced with second degree burn wound. This research was a laboratory experimental study using 18 male white rats divided into control positive (K+) group, bioplacenton (P1) and chicken yolk oil (P2). Observation of the re-epithelialization, granulation and inflammatory cells infiltration were carried out 14 days after the rat were induced with second degree burn wound. The necropcy was performed on the 14th day and then the skin organs were treated with HE staining and observed under a microscope. Data analysis was performed using Kruskal-Wallis followed by Mann Whitney. The results of the analysis showed that K + had significantly different results (P <0.05) compared to P1 and P2 in the scores of re-epithelialization, granulation and the number of inflammatory cell infiltrations. P1 and P2 were not significantly different (P> 0.05). In this study, it can be concluded that chicken egg yolk has a comparable effect with bioplacenton toward the score of re-epithelialization, granulation and the number of inflammatory cell infiltration.