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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Indonesian Journal of Applied Research (IJAR) Jurnal Nukleus Peternakan Jurnal Veteriner dan Biomedis Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Dedi Rahmat Setiadi
Departemen Klinik, Reproduksi, Dan Patologi; Fakultas Kedokteran Hewan, Institut Pertanian Bogor (IPB); Jalan Agatis Kampus IPB Dramaga, Bogor, Jawa Barat, INDONESIA, Kode Pos 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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EFFECTIVENESS OF VARIOUS COMMERCIAL EXTENDERS IN CRYOPRESERVING SEMEN OF LOCAL INDONESIAN RAMS Setiadi, Dedi Rahmat; Magfira; Riwu, Ronny Markus Jami; Arifiantini, R Iis
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9208

Abstract

Indonesian local sheep represent a particular species of sheep reared by farmers on small-scale farms. Semen freezing can be performed using either a homemade or commercial extender. The present study evaluated the effectiveness of several commercial extenders in freezing Indonesian local sheep. Semen was collected from three mature and healthy rams using an artificial vagina and evaluated. It was determined that only samples exhibiting sperm motility levels of greater than 70% would be included in the study. Subsequently, the semen from each ram was divided into three portions and diluted with Andromed, Sterydil, and OptiXcell, respectively. The frozen semen was then evaluated 24 hours post-freezing. The thawing process was conducted individually at 37°C for 30 seconds. The evaluation encompassed a range of metrics, including sperm motility, viability, and the presence of any abnormalities, in addition to the recovery rate. The results indicated that sperm frozen in Optixcell and Sterydil extenders exhibited superior motility, viability, and recovery rate than Andromed. The study also demonstrated no interaction between individual Ram and extender, and no differences were found between individuals. The study concluded that Optixcell and Sterydil extenders effectively froze Indonesian local sheep semen.
Kualitas Estrus pada Domba Garut Setelah Sinkronisasi Estrus Menggunakan Prostaglandin, Progesterone dan Kombinasinya Cristóvão, Adelaide José Pereira; Setiadi, Mohamad Agus; Setiadi, Dedi Rahmat; Mohamad Agus Setiadi
Acta VETERINARIA Indonesiana Vol. 13 No. 3 (2025): November 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.3.286-295

Abstract

Estrus synchronization is a technique used to stimulate estrus and ovulation in a group of animals using hormones. This study aimed to compare three estrus synchronization methods. Fifteen female garut sheep were used and treated with: progesterone-CIDR, double injection prostaglandins, and their combination. At standing heat, ewes were mated with a buck. The parameters measured include the onset estrus, duration estrus, pattern of electrical impedance in the vaginal mucus, number and size of follicles, and pregnancy status. All treated ewes responded to estrus (100%). There were different onset of estrus, namely CIDR (54h), CIDR+PGF2α (41h), and double injection of PGF2α (49.4h), respectively. Meanwhile, the Duration of estrus of CIDR (30,6h), CIDR+PGF2α (29.8h), and double injection of PGF2α (22.4h), respectively. Furthermore, electrical impedance of vaginal mucus at standing heat were CIDR (208Ω), CIDR+PGF2α (189Ω) and double injection of PGF2α (305Ω). The follicle size on H0 to standing heat in each group was significant (p<0.05), while the follicle count was significant only (p<0.05) in the CIDR group. Pregnancy rate was achieved by synchronization with CIDR and double injection prostaglandin (100%) compared to CIDR+PGF2α (40%). It's concluded that the third method of synchronization could result in the best estrus response with slight different characteristics.
Co-Authors Abdul Samik Afiff , Usamah Amani, Aqila Zata Amrozi Anriansyah Renggaman Bayu Febram Prasetyo Budianto Panjaitan Chairun Nisa Cristovao, Adelaide Jose Pereira Danang Dwi Cahyadi Dea Indriani Astuti Didid Diapari Dondin Sajuthi Fatimah Fatimah Handri Dwi Agung Hastuti, Yohana Tri Heru Setijanto Iis Afriantini Iman Supriatna Jansen Manansang Jansen Manansang Laksono, Mohamad Nanang Tejo M Agus Setiadi Magfira Manansang, Willem Mokhamad Fakhrul Ulum, Mokhamad Muchidin Noordin Muchidin Noordin MUHAMMAD AGIL Mulia, Bongot Huaso Nurhidayat Putri Syifa Camilla R. Iis Arifiantini Raden I. Arifiantini Riwu, Ronny Markus Jami Savitri Novelina Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Srihadi Agungpriyono Supratikno . Surya Agus Prihatno Tuty L Yusuf Vincentia Trisna Yoelinda Yohana Tri Hastuti Yohana Tri Hastuti Yosua Kristian Adi