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All Journal Jurnal Akuakultur Indonesia Jurnal Pertanian Terpadu Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Aquacultura Indonesiana MARLIN : Marine and Fisheries Science Technology Journal SIGANUS: Journal of Fisheries and Marine Science Journal of Multidisciplinary Applied Natural Science Journal of Fisheries & Marine
ALIM ISNANSETYO
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

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Extraction and Fractionation of Anti-Cholesterol Compounds from Water Chestnut (Eleocharis dulcis) Putra, Hemi Adi; Husni, Amir; Isnansetyo, Alim
Jurnal Perikanan Universitas Gadjah Mada Vol 27, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.106066

Abstract

Cholesterol is a significant contributor to mortality rates worldwide. The quest for effective cholesterol treatments continues, aiming to develop preventive agents with minimal adverse effects. This study seeks to elucidate the anti-cholesterol properties of extracts and components fractionated from water chestnut (Eleocharis dulcis). The research encompassed several phases, including sampling, extraction and fractionation, qualitative and quantitative flavonoid analysis, anti-cholesterol activity assays, and gas chromatography-mass spectrometry (GC-MS) analysis. The quantitative flavonoid assessment revealed that the highest flavonoid concentration was present in the ethyl acetate:methanol (5:5) fraction, totaling 79.224 mg/mL. The water chestnut extract demonstrated notable anti-cholesterol activity with an IC50 value of 13016.27 ± 5648.31 ppm, whereas the water chestnut fraction exhibited enhanced anti-cholesterol activity with an IC50 value of 42.22 ± 9.79 ppm, comparable to simvastatin. The bioactive compounds in water chestnut attributed to its anti-cholesterol effects include 7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, n-Hexadecanoic acid, Octadecanoic acid ethyl ester, 9-Octadecenamide (Z)-, 9,12,15-Octadecatrienoic acid, 2,3-dihydroxypropyl ester, 3,4-Dimethoxycinnamic acid, and 2H-1-Benzopyran-2-one, 7-methoxy-. These findings suggest that the fraction derived from E. dulcis exhibits significant anti-cholesterol activity and holds promise for further development in the treatment of hypercholesterolemia.
Susceptibility and Target Organ of Lymphocystis Disease Virus Infection in Giant Gourami (Osphronemus goramy), Hybrid Tilapia (Oreochromis sp.), Siamese Fighting Fish (Betta splendens), and Hybrid Catfish (Clarias sp.) Nikmah, Nur Lailatul Fitrotun; Isnansetyo, Alim; Istiqomah, Indah; Murwantoko, Murwantoko
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.56685

Abstract

  Highlight Research 1. Lymphocystis disease is reported to infect seawater and freshwater fishes 2. The four important freshwater fish species in Indonesia are evaluated on their susceptibility to Lymphocystis Disease Virus 3. Lymphocystis infection causes behavioural changes and mortality with different onset times after infection on the four fish species 4. LCDV load is varied in quantity among different organs. Abstract Lymphocystis disease has a broad host range and has been reported to enter Indonesia. However, information regarding its susceptibility and predilection organs in fish is lacking. This study examined the susceptibility of four important fish species in Indonesia, namely, giant gourami (Osphronemus goramy), hybrid tilapia (Oreochromis sp.), Siamese fighting fish (Betta splendens), and hybrid catfish (Clarias sp.). The fish were infected with virus filtrate by intraperitoneal injection and immersion. The postinfection observation period was 60 days. Viral load was quantified by qPCR and expressed as major capsid protein (MCP) copy number/mg tissue. Mortality was observed in all fish species, with the highest recorded in hybrid catfish and the lowest in Siamese fighting fish. All the fish species showed changes in their clinical symptoms, such as anorexia and separation from schools. However, only giant gourami showed internal change seven days after injection (dpi), with white lesion detected in the liver. Viral load quantification showed that LCDV had different predilection organs in the four fish species. The highest viral load of giant gourami (1.7 x 104) was observed in the liver at 7 dpi, hybrid tilapia (7.5 x 103) was observed in the fins at 21 dpi, Siamese fighting fish (8.4 x 103) was observed in the fins at 14 dpi, and hybrid catfish (1.2 x 103) were observed in the fins and gills at 7 and 14 dpi. The findings indicated that giant gourami, hybrid tilapia, Siamese fighting fish, and hybrid catfish were susceptible to LCDV infection with different predilection organs.
PEMBERIAN FUCOIDAN SECARA ORAL DARI HASIL EKSTRAKSI Sargassum sp. UNTUK MENANGGULANGI MOTILE AEROMONAS SEPTICEMIA PADA IKAN LELE (Clarias sp.) Muahiddah, Nuri; Isnansetyo, Alim
Jurnal Riset Akuakultur Vol 18, No 3 (2023): (September, 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.3.2023.197-206

Abstract

Motile aeromonas septicemia (MAS) adalah salah satu penyakit yang sering menyebabkan kematian pada ikan, dan disebabkan oleh bakteri Aeromonas hydrophila. Salah satu cara untuk menanggulangi penyakit bakteri yang menyerang ikan adalah dengan menggunakan imunostimulan. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian fucoidan secara oral terhadap perkembangan gejala penyakit ikan lele yang diinfeksi A. hydrophila.  Penggunaan metode asam digunakan untuk mengekstraksi fucoidan dari Sargassum sp. Penelitian ini melibatkan empat perlakuan dan tiga ulangan. Perlakuan P1, P2, P3, dan P4, masing-masing meliputi kontrol, 2, 4, dan 6 g fucoidan per kilogram pakan. Pakan diberikan kepada ikan dalam jumlah 5% dari berat kering pakan terhadap biomassa ikan. Setelah itu, dilakukan uji tantang dengan memberikan dosis 0,1 mL per ikan dari A. hydrophila dengan kepadatan 2,77 x 107 sel per ml secara intraperitonial satu minggu setelah pemberian fucoidan secara oral. Hasil penelitian menunjukkan bahwa pemberian fucoidan secara oral dengan dosis 2, 4, dan 6 g fucoidan per kilogram pakan tidak menghasilkan peningkatan yang signifikan dalam sintasan (P>0,05) dan tidak mampu menunda MTD pada ikan yang terinfeksi A. hydrophila KP1 jika infeksi dilakukan di bawah LD50. Perlu dilakukan penelitian lebih lanjut dengan menambahkan dosis fucoidan pada pakan ikan lele untuk mendapatkan hasil yang lebih baik.Motile aeromonas septicemia (MAS) is a disease that often causes death in fish, and is caused by Aeromonas hydrophila. One of ways to overcome bacterial diseases that attack fish is to use immunostimulants. The aim of this study was to determine the effect of oral administration of fucoidan on the development of disease symptoms in catfish infected with A. hydrophila. The acid method was used to extract fucoidan from Sargassum sp. This experiment involved four treatments and three replications. Treatments P1, P2, P3, and P4, respectively, included control, 2, 4, and 6 g of fucoidan per kilogram of feed. Feed was given to fish in an amount of 5% of the dry weight of the feed to fish biomass. After that, a challenge test was carried out by giving a dose of 0.1 mL per fish of A. hydrophila with a density of 2.77 x 107 cells per mL intraperitoneally one week after oral administration of fucoidan. The results showed that oral administration of fucoidan at doses of 2, 4, and 6 g of fucoidan per kilogram of feed did not result in a significant increase in survival (P>0.05) and was unable to delay MTD in fish infected with A. hydrophila KP1 if infection carried out below LD50. Further study needs to be done by adding a dose of fucoidan to catfish feed to get better results. 
AKTIVITAS ANTIBAKTERI TUMBUHAN DARAT DAN PESISIR DARI SULAWESI SELATAN TERHADAP PENYAKIT VIBRIO Basir, Buana; Kariyanti, Kariyanti; Isnansetyo, Alim
MARLIN Vol 4, No 1 (2023): (Februari) 2023
Publisher : Politeknik Kelautan dan Perikanan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/marlin.V4.I1.2023.49-56

Abstract

Salah satu alternatif penanganan penyakit bakteri Vibrio sp. adalah dengan penggunaan bahan bioaktif alami dari tumbuhan yang dapat menggantikan peran antibiotik Penelitian bertujuan untuk menganalisis aktivitas antibakteri tumbuhan darat dan pesisir dari Sulawesi Selatan terhadap bakteri Vibrio sp.. Penelitian dilaksanakan pada bulan April sampai dengan Agustus 2018, dengan pengambilan sampel uji di daerah Sulawesi Selatan. Penelitian dilakukan dengan metode percobaan dengan beberapa tahapan, yaitu persiapan sampel, maserasi sampel, partisi, dan uji aktivitas antibakteri. Sampel yang diuji sebanyak 30 sampel yang berasal dari 26 jenis tanaman dengan konsentrasi 500 µg/mL dan 1000 µg/mL. Jenis vibrio yang digunakan adalah V. parahaemolitycus, V. harveyii, dan V. alginolitycus. Analisis data dilakukan secara kualitatif berdasarkan zona hambat yang terbentuk. Berdasarkan hasil uji aktivitas antibakteri dari crude ekstrak ditemukan enam sampel yang memiliki aktivitas anti-vibrio. yaitu daun sukun, kulit buah sukun, kulit batang sukun. daun jara, daun avicennia, dan daun miana, dengan masing-masing nilai aktivitas sebesar 12 mm, 10 mm, 9 mm, 12,5 mm, 16 mm, dan 19 mm. Aktivitas antibakteri tertinggi dihasilkan oleh crude ekstrak daun miana pada fraksi etanol sebesar 23-24 mm.One alternative treatment for the bacterial disease is Vibrio sp. with natural bioactive ingredients from plants that can replace the role of antibiotics. The research aimed to analyze the antibacterial activity of miana leaves against Vibrio sp. The research was carried out from April to August 2018, by taking test samples in the South Sulawesi area. The study was conducted using an experimental method with several stages, sample preparation, sample maceration, partitioning, and antibacterial activity test. The samples tested were 30 samples from 26 types of plants with concentrations of 500 µg/mL and 1000 µg/mL. The types of vibrios used were V. parahaemolitycus, V. harveyii, and V. alginolitycus. Data analysis was carried out qualitatively based on the inhibition zones formed. Based on the results of the antibacterial activity test of the crude extract, it was found that six samples had anti-vibrio activity namely breadfruit leaves, breadfruit skin, breadfruit bark. Jara leaves, Avicennia leaves, and Miana leaves, with activity values of 12 mm, 10 mm, 9 mm, 12.5 mm, 16 mm, and 19 mm, respectively. Crude miana leaf extract had the highest antibacterial activity 23-24 mm ethanol production.
Uji Aerasi Microbubble dalam Menentukan Kualitas Air, Nilai Nutrition Value Coefficient (NVC), Faktor Kondisi (K) dan Performa pada Budidaya Nila Merah (Oreocrhomis Sp.) Heriyati, Eny; Rustadi, Rustadi; Isnansetyo, alim; Triyatmo, Bambang
Jurnal Pertanian Terpadu Vol 8 No 1 (2020): Jurnal Pertanian Terpadu Jilid VIII Nomor 1 Juni 2020
Publisher : Sekolah Tinggi Pertanian Kutai Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36084/jpt..v8i1.232

Abstract

Penelitian ini betujuan untuk menguji penggunaan aerasi microbubble pada parameter kualitas air, performa, nilai NVC dan factor kondisi perairan. Ikan berukuran 12±3 g, sebanyak 50 ekor dipelihara selama 3 bulan dengan perlakuan aerasi micobubble, aerasi konvensional dan non aerasi dalam system resirkulasi. Parameter yang dianalisa dalam penelitian ini menunjukkan nilai DO aerasi microbubble lebih tinggi dan tetap bertahan lama sampai akhir penelitian dibandingkan aerasi konvensional dan control (p<0,05), demikian juga dengan suhu air. Parameter kualitas air lainnya tidak dipengaruhi oleh perlakuan aerasi, dan masih menunjukkan nilai yang normal untuk budidaya nila, kecuali ammonia pada semua perlakuan menunjukkan nilai yang melebihi standar SNI. Pengaruh stabilnya nilai DO yang dihasilkan oleh aerasi microbubble berpengaruh pada performa nila, yang mampu meningkatkan ukuran bobot ikan tiap ekor dan meningkatkan biomasa ikan 268 % dari kontrol dan 32,5 % lebih tinggi aerasi konvensional. Dalam penelitian ini nilai factor kondisi dan NVC dari semua perlakuan menunjukkan nilai yang relative sama. Kesimpulan dari penelitian ini adalah, meskipun nilai kondisi dan NVC tidak dipengaruhi perlakuan aerasi, namun aerasi microbubble mampu meningkatkan DO, pertumbuhan dan biomasa ikan.
Comparative Study of Pre-Extraction Treatments and Buffer Modifications for Macroalgal Lectin Extraction Fajarningsih, Nurrahmi Dewi; Nursid, Muhammad; Nugroho, Hartanto; Nuringtyas, Tri Rini; Isnansetyo, Alim
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 3 (2023): December 2023
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.852

Abstract

Getting an efficient extraction approach is a crucial step in bioactive protein research, particularly lectin. This research aimed to examine the efficiency of cryogenic-grinding (CG) and freeze-dried-grinding (FG) pre-extraction treatments, and also the incorporation of phenylmethylsulphonyl fluoride (PMSF), Tween 80, polyvinylpolypyrrolidone (PVPP), 70% Ethanol (EtOH), or combination of the chemicals in the 20 mM phosphate buffered saline pH 7 (PBS) for extracting lectin from Ulva lactuca, Sargassum polycystum, and Hydropuntia edulis. The lectin content of the extracts was determined using the hemagglutination activity (HA) assay. The phenolic content was measured to determine its impact on the lectins’ HA. Lectin extraction efficiency was determined by analyzing the extracts’ minimum agglutination concentration (MAC) and total hemagglutination activity (THA). CG pre-extraction treatment produced slightly higher THA than FG, making it slightly more efficient. The EtOH treatment efficiently extracted lectin from U. lactuca and H. edulis by substantially reducing the polyphenol (PPs) content, lowering the MAC, and increasing the THA. The EtOH treatment significantly decreased the PPs and HA of the S. polycystum, suggesting that the HA is most likely produced by the PPs rather than the lectin content. Tween 80 raised the THA of U. lactuca by 17-fold with native rabbit erythrocyte compared to the control but did not affect the THA of H. edulis and S. polycystum. Several different effects of chemicals incorporated in the extraction buffers suggested that the optimum macroalgal lectin extraction strategy is species-dependent.
Modulation of Gut Microbiota, Nutrient Transport Gene Expression, and Growth of Red Hybrid Tilapia (Oreochromis sp.) Fed with Black Soldier Fly (Hermetia illucens) by Oral Probiotics Latifah, Ma'rufa Nurul; Murti, Fauziah Arelia Usmiara; Helmiati, Senny; Ismail, Norshida; Syakuri, Hamdan; Istiqomah, Indah; Murwantoko, Murwantoko; Isnansetyo, Alim
Jurnal Perikanan Universitas Gadjah Mada Vol 26, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.95268

Abstract

Maggot Black Soldier Fly (BSF) is a nutritionally rich alternative food for fish. The slow digestibility requires the combination with other technologies to produce fish with excellent growth. This study aims to investigate the impact of probiotic application on the growth, microbiome, and gene expression in the intestines of tilapia-fed black soldier fly (BSF) larvae. Tilapia intestines were examined after two months of rearing with three replications and two treatments: A) 30% maggots and 70% commercial pellets with probiotics, and B) 30% maggots and 70% commercial pellets without probiotics. We discovered that probiotics have a significant impact on the gut microbiomes of fish and their absolute growth. The amplicon sequence variant in the probiotic treatment (A) was 25, with dominance by Cetobacterium, Acinetobacter, Enhydrobacter, and Gemmobacter, while the non-probiotic treatment (B) was 8, with dominance by Cetobacterium and Turicibacter. The probiotic treatment increased the expression of Ghrelin, Muc-2, IL-1β, and I-FABP genes, but not the CD36 gene. These findings suggest oral probiotics can help boost tilapia production when fed maggot black soldier fly (Hermetia illucens).
Co-Authors Abdullah, Faisal Zain Amir Husni Angela Mariana Lusiastuti Anshory, Lutfi Bambang Triyatmo Buana Basir Condro, Sri Laksono Eny Heriyati, Eny Esti Handayani Hardi Gina Saptiani Halim, Aldi M. Hariyadi, Dimas Rizky Ign. Hardaningsih INDAH ISTIQOMAH Irpani, Husni Mubarok Ismail, Norshida Jabbar, Firmansyah Bin Abd Kariyanti, Kariyanti Kim, Sung Sam Kristiani, Theresia Adven Dea Latifah, Ma'rufa Nurul Lusiana, Lusiana Mira Mawardi Muhammad Nursid Mukhlisnah Djalil Murti, Fauziah Arelia Usmiara Murwantoko . Niklani, Andi Nikmah, Nur Lailatul Fitrotun Noer Kasanah Nugroho, Hartanto Nuri Muahiddah Nurrahmi Dewi Fajarningsih, Nurrahmi Dewi Putra, Hemi Adi Rahayu, Widianingsih Rarastoeti Pratiwi Rina Puji Astuti Rudy Agung Nugroho Rustadi Rustadi Senny Helmiati SUWARNO HADISUSANTO Syakuri, Hamdan Tito Arya Nugraha Tri Rini Nuringtyas Triastutik, Gemi Triyanto Triyanto Wahyudi Wahyudi Wulansari, Tyas Ayu Yasa, Ngurah Sedana Yasa, Ngurah Sedana Zafran, Zafran