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Identification of Causes of Mass Death Of Gurami Fish (Osphronemus gouramy) In Gurami Fish Cultivation Sentra, Desa Beji, Kedung Banteng District, Banyumas District, Central Java Khumaidi, Achmad; Hidayat, Aris
Journal of Aquaculture Science Vol 3, No 2 (2018): Journal of Aquaculture Science
Publisher : Airlangga University

Kasus kematian massal ikan Gurami di Sentra Budidaya Ikan Gurami, Desa Beji, Kecamatan Kedung Banteng, Kabupaten Banyumas, Jawa Tengah berdampak pada menurunnya produksi telur dan benih ikan Gurami. Penelitian ini bertujuan untuk mengidentifikasi patogen penyebab kematian pada ikan Gurami. Metode yang digunakan metode survei dengan pengambilan sampel secara acak, kemudian diidentifikasi keberadaan patogen pada sampel ikan Gurami. Hasil identifikasi patogen pada ikan Gurami menunjukkan adanya infeksi Bakteri (Aeromonas hydrophila, Aeromonas sobria), Parasit (Henneguya sp., Trichodina sp., Vorticella sp.) dan Jamur (Aspergillus sp.). Penelitian ini menunjukkan bahwa kematian massal pada ikan Gurami disebabkan oleh serangan bakteri, parasit, dan jamur.Kata Kunci: ikan Gurami, kematian massal, bakteri, parasit, jamur.

Insilico Prediction of Biological Activity of Secondary Metabolites of Amphora sp.: for Development of Natural Medicines Khumaidi, Ach.; Wafi, Abdul; Muqsith, Abdul
Journal of Aquaculture Science Vol 6, No 1IS (2021): Vol 6 Issue Spesial 2021 Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v6i1IS.163

Diatom Amphora sp. is a type of aquaculture product that has various types of metabolites with very good potential to be used as basic ingredients for drugs or other health products. Insilico analysis of extracts of Amphora sp. was carried out to predict biological activity that can be used as a basis for developing the use of aquaculture products. The research was conducted by extracting Amphora sp. using ethanol solvent with sonication method, the extraction results were analyzed by LC-HRMS, and the metabolite readings were then analyzed for drug potential and biological activity online using swissADME, Protox II, and Way2Drug applications. The results of the LC-HRMS analysis showed that the extracted Amphora sp. showed the highest abundance of valine compounds with 17.6% as well as other compounds L-Norleucin, stearamide, palmitoleic acid, isotretinoin, and arachidonic acid. The results of the ADME/T analysis showed that the six compounds of Amphora sp. extract had quite good potential according to the Lipinski rule of five. Biological activity also shows potential as an anti-inflammatory, antifungal, antibacterial, antiviral, and antineoplastic agent with Pa values ranging from 0.260 to 0.873. These results indicate that the production development of Amphora sp. and its use is very wide, including in the health sector.Keyword: ADME/T, PASSonline, Amphora sp., sonication, aquaculture

POTENSI ANTIOKSIDAN EKSTRAK METANOL DIATOM Amphora sp. Ach. Khumaidi; Astik Umiyah; Abdul Muqsith; Abdul Wafi
Jurnal Akuakultur Rawa Indonesia Vol 9, No 1 (2021): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v9i1.13687

Diatoms are a type of microalgae with a quite strong potential in the health sector as antioxidants, anticancer, and antivirals. But until now in Indonesia is still very rarely carried out research on the antioxidant potential of diatoms. The purpose of this study was to determine the antioxidant potential of Amphora sp. extracted with methanol. The method used in the extraction process is maceration, with a ratio of sample and methanol 1: 3 (300 g: 900 ml). The evaporation process compacted maserate resulting from maceration, then analyzed the total chlorophyll content, total flavonoids, and total phenols. Amphora sp. extraction results showed good potential with 7.08 ppm chlorophyll content, total flavonoids 6,299 ppm, and total phenol 7,085 ppm, and IC50 DPPH values of 3332.5 ppm. The IC50 value of the extract of Amphora sp. still classified as very weak antioxidant activity, but in general the extract of Amphora sp. has other bioactive potentials such as chlorophyll, flavonoids, and total phenol which can be used as material for further studies for the development of natural medicinal ingredients from Amphora sp.Key words: antioxidant, diatome, DPPH, macaration, methanol.

PENINGKATAN PRODUKTIVITAS IKAN SIDAT(Anguila sp.)DENGAN SISTEM POLIKULTUR Gagan Garnawansah; Wulandari Suryaningtyas; Ach. Khumaidi
Samakia : Jurnal Ilmu Perikanan Vol 8 No 1 (2017): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Eel (Anguilla sp.) is an export commodity that has high economic value, but in the development of cultivation encountered a problem on the treatment period. To reach consumption size it takes 12-16 months. To improve productivity and provide added value needs polyculture systems with tilapia which is easy in terms of its treatment and can stabilize water quality. The aim of this study was to determine the productivity of eels using polyculture systems with tilapia. The research was conducted at BLUPPB Karawang in February – April 2015, using six (6) pool of treatment. Three pools were used for activities with a polyculture system and three pools were used for the treatment of non-polyculture. The results showed that a polyculture system of eel and tilapia treated for 60 days can make a positive impact to the Specific Grow Rate (SGR) 0.98%, and Food Conversion Ratio (FCR) 1.09 when compared with non-polyculture with SGR (0,93) and FCR (1.23). The results of analysis of effort on polyculture systems can provide the added value of the tilapia harvest.

TEKNIK PEMBENIHAN IKAN MAS(Cyprinus carpio,L) di BALAI BENIH IKAN (BBI) TENGGARANG BONDOWOSO Ismail Ismail; Ach. Khumaidi
Samakia : Jurnal Ilmu Perikanan Vol 7 No 1 (2016): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Carp (Cyprinus carpio, L.) is a species of freshwater fish that has long been cultivated and domesticated well in the world. In China, farmers have cultivated around 4,000 years ago, while in Europe several hundred years ago. Some fish varieties and subvarietas Mas Southeast Asia has been widely cultivated as a food fish and ornamental fish. C. carpio spawn at 22.00 until dawn marked with fish activity male chasing the female parent. When it rains fish usually spawn faster. Mas process of spawning fish is the female parent will release eggs while keeping up with the male parent where the male parent while it also spray his sperm on the egg. It is important also pursued when the activity takes place the water continues to flow to supply oxygen dissolved in the fish kolam.Pembenihan Mas can be done in the traditional way, semi-intensive and intensive. The mortality rate in the hatchery can reach 10-20%, it is because the eggs did not hatch due to the muddy water that covered the surface of the egg. Consequently affect hatching, each pool must be no drain water income and expenditure.

MIKROALGA LAUT Nannochloropsis Oculata SEBAGAI ALTERNATIF ANTIVIRUS Viral Nervous Necrotic (VNN) PADAIKAN KERAPU TIKUS (Cromileptes Altivelis) Ach. Khumaidi
Samakia : Jurnal Ilmu Perikanan Vol 7 No 1 (2016): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Viral nervous necrosis (VNN) is the causative agent of the disease that quite dangerous for the survival of grouper farming (C. altivelis). Impacts this virus attacks are capable of causing up to 100% mortality. For overcome attack VNN can be used materials of biological resources is N. oculata. Utilization marine microalgae N. oculata were able to improve the immune system, boost the immune response by improving the expression of Toll-like Receptor (TLR) expression Major Histocompitability Complex (MHC), were able to inducing antiviral genes (P56), and able to provide an anti-inflammatory effect. With these capabilities. Feasible to develop the utilization of marine microalgae N. oculata as an alternative to tackling VNN attack on C. altivelis.

MANAGEMENT OF HATCHERY PRODUCTION VANAME (Litopenaeus vannamei) NAUPLI IN INSTALLATION OF SHRIMP (IPU) GELUNG BRACKISH WATER AQUACULTURE CENTRE (BPBAP) SITUBONDO EAST JAVA Choirul Anam; Ach. Khumaidi; Abdul Muqsith
Samakia : Jurnal Ilmu Perikanan Vol 7 No 2 (2016): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Vaname (Litopenaeus vannamei) is a commodity brackish water, which has been much in demand and become a superior product aquaculture sector in Indonesia. This is because some of the advantages possessed by vaname prawns, which are can be maintained with high stocking density, rapid growth, has a high resistance to disease and environmental change. In its natural habitat, the shrimp vaname usually make the process of mating at night time, but the most active usually occurs at sunset. The mating process occurs in four stages, namely the approach, chase, perangkakan and mating. This process can be seen by looking at the behavior of the male parent who swim with the female parent. Both of these stem looks like a chase. Then swim parallel to the male parent and a female parent turned toward ventral female shrimp. After that the male parent and a female parent gripping releasing sperm attached to thellycum. In the larval rearing, which must be considered is the management of water quality and feeding management. This is because water is a live media aquatic organism that is crucial to the survival rite larvae. Besides feeding management also determine the success or failure of an aquaculture business where feed costs reached 60-75% of total production costs.

Potential Antivirus Viral Nervous Necrosis Methanol extract of Amphora sp. in Cantang Grouper (Epinephelus sp.) Ach. Khumaidi; Astik Umiyah
Samakia : Jurnal Ilmu Perikanan Vol 10 No 2 (2019): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Cantang grouper received more serious attention from grouper fish farmers due to high export interest, but the attack of viral nervous necrosis (VNN) became a major obstacle in its cultivation. This study aims to explore the antiviral potential of diatom Amphora sp. to counter the VNN attack on Cantang groupers. The method used is the extraction of Amphora sp. with absolute methanol solvent. The extraction results were tested in vivo by giving Amphora sp. with different concentrations, namely: 17 µg / ml, 33 μg / ml, 50 μg / ml, and administration of extracts by challenging the 17 μg / ml + VNN, 33 μg / ml + VNN, 50 μg / ml + VNN. Fish treated with Amphora sp. also challenged by giving VNN Positive fish meat. During the period of rearing fish observed clinical behavior and symptoms. After the fish were raised for 15 days, the fish harvested were analyzed using histology, RT-PCR, and CPI methods (using the immuno ratio software) to see the HSP immune response obtained from the administration of Amphora sp. to find out its potential as a natural antivirus. From several test parameters, the concentration of extract 50 mg / ml + VNN was given to give the best response in the CPI analysis with a DAB value (61.3%). These results indicate that the methanol extract of Amphora sp. has the potential to be used as an antiviral candidate in Cantang grouper fish.

Pemetaan Kesesuaian Lahan Budidaya Rumput Laut Di Kecamatan Banyuputih, Situbondo Berdasarkan Indikator Kimia Air Abdul Wafi; Heri Ariadi; Ach Khumaidi; Abdul Muqsith
Samakia : Jurnal Ilmu Perikanan Vol 12 No 2 (2021): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Budidaya rumput laut adalah salah satu sub-kegiatan akuakultur yang sangat potensial untuk dikembangkan di Kecamatan Banyuputih, Situbondo. Tujuan dari penelitian ini adalah untuk memetakan kesesuaian lahan budidaya di Kecamatan Banyuputih Situbondo guna dapat digunakan sebagai area budidaya rumput laut berdasarkan nilai indikator parameter kimia air yang ada. Penelitian ini dilaksanakan di perairan pesisir Kecamatan Banyuputih, Situbondo selama bulan januari-maret 2019 dengan konsep penelitian lapang dan analisa spasial dari pengambilan data kimia air (oksigen terlarut, fosfat, dan nitrat) yang kemudian dianalisis menggunakan metode Inverse Distance Weighting (IDW) dan overlay pada aplikasi GIS (Geographic Information System) guna mendapatkan model visualisasi peta tematik kesesuaian lahan. Hasil penelitian menunjukan parameter oksigen terlarut, fosfat, serta nitrat berfluktuasi secara variatif dan dinamis selama masa penelitian berlangsung, dengan kisaran konsentrasi 5.4-6.1 mg/L (DO), 0.8-1.6 mg/L (PO4), dan 2.1-3.7 mg/L (NO3). Kondisi tersebut, menandakan bahwa perairan pesisir Kecamatan Banyuputih cenderung subur dan layak untuk digunakan sebagai lahan budidaya akuakultur. Pernyataan tersebut juga bisa dilihat dari hasil visualisasi warna pada gambar kesesuaian lahan di peta tematik peneltian. Nilai konsentrasi dari parameter DO (5.4-6.1 mg/L), fosfat (0.8-1.6 mg/L), dan nitrat (2.1-3.7 mg/L) di perairan Kecamatan Banyuputih juga masih sesuai dengan ambang batas baku mutu kualitas air yang diperuntukan untuk budidaya rumput laut. Sehingga dari penelitian ini dapat disimpulkan, dari berbagai gambar peta tematik hasil analisis spasial berdasarkan indeks parameter kimia air yang ada, lokasi perairan Kecamatan Banyuputih sangat layak dan potensial untuk dikembangkan sebagai area budidaya rumput laut yang produktif.

Identification of Causes of Mass Death Of Gurami Fish (Osphronemus gouramy) In Gurami Fish Cultivation Sentra, Desa Beji, Kedung Banteng District, Banyumas District, Central Java Achmad Khumaidi; Aris Hidayat
Journal of Aquaculture Science Vol 3 No 2 (2018): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v3i2.53

Kasus kematian massal ikan Gurami di Sentra Budidaya Ikan Gurami, Desa Beji, Kecamatan Kedung Banteng, Kabupaten Banyumas, Jawa Tengah berdampak pada menurunnya produksi telur dan benih ikan Gurami. Penelitian ini bertujuan untuk mengidentifikasi patogen penyebab kematian pada ikan Gurami. Metode yang digunakan metode survei dengan pengambilan sampel secara acak, kemudian diidentifikasi keberadaan patogen pada sampel ikan Gurami. Hasil identifikasi patogen pada ikan Gurami menunjukkan adanya infeksi Bakteri (Aeromonas hydrophila, Aeromonas sobria), Parasit (Henneguya sp., Trichodina sp., Vorticella sp.) dan Jamur (Aspergillus sp.). Penelitian ini menunjukkan bahwa kematian massal pada ikan Gurami disebabkan oleh serangan bakteri, parasit, dan jamur.Kata Kunci: ikan Gurami, kematian massal, bakteri, parasit, jamur.

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