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All Journal Jurnal Akuakultur Indonesia Jurnal Akuakultur Rawa Indonesia Journal of Aquatropica Asia Samakia: Jurnal Ilmu Perikanan Journal of Aquaculture and Fish Health INTEGRITAS : Jurnal Pengabdian Jurnal Perikanan Pantura As-Sidanah : Jurnal Pengabdian Masyarakat Journal of Fisheries & Marine Jurnal Perikanan Journal of Aquaculture Science
Ach. Khumaidi
Faculty Of Science And Technology, University Of Ibrahimy, Situbondo, East Java
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Social Sciences Other

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KAJIAN TEKNIS PENDEDERAN IKAN KERAPU CANTANG (Epinephelus fuscoguttatus >< Epinephelus lanceolatus) SECARA INTENSIF DI BPBAP SITUBONDO Khumaidi, Ach.; Faizin, Rohmanul; Prakosa, Dimas Galang
Journal of Aquatropica Asia Vol 7 No 2 (2022): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/joaa.v7i2.3420

Abstract

Grouper production in Indonesia can be supported by hybridization technology which can produce grouper species with relatively faster growth and more resistance to disease. Cantang grouper is a type of grouper fish that was engineered by crossbreeding between female tiger grouper (Epinephelus fuscoguttatus) and male grouper Kertang (Epinephelus lanceolatus). The purpose of this study was to determine the stages and technology of nursery grouper Cantang which was carried out at BPBAP Situbondo. The method used in this research is the method of observation, participation, and interviews. The primary data needed in this study are data on infrastructure, data on the number and size of seeds, feed management, water quality management, and harvesting. The data is presented with a descriptive method by discussing the data that has been obtained compared to the theories that have developed at this time. The results of this study indicate that cantang grouper nursery activities start from tank preparation, water filling, seed selection and distribution, feed management, water quality, fish pest and disease control, grading, and harvesting of cantang grouper seeds. During the practice period, the seeds were spread from hatcheries with a stocking density of 3000 to 5000 individuals in one tank with a size of 4 x 2.5 x 1 m, or the equivalent of 300 to 500 individuals/m3. The distribution of seeds is carried out after the acclimatization process, namely the adaptation process before releasing the seeds. After the seeds are removed, the tub is given an antiseptic to prevent disease in the seeds. There are several factors that can affect the growth rate of seeds, namely water quality management and feeding. During the maintenance period, a flowing water system is used, so that the water used is continuously renewed. In the morning, the seeds are flushed and soaked using fresh water, with the aim of releasing mucus on the seeds so as to reduce the potential for disease outbreaks. Nursery grouper at BPBAP Situbondo can run optimally by paying attention to the stages of cultivation, especially in the feed management and water quality management.
The application of pigment-protein fraction from Nannochloropsis oculata on β-actin response of Cromileptes altivelis infected with viral nervous necrosis Yanuhar, Uun; Khumaidi, Achmad
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3894.518 KB) | DOI: 10.19027/jai.16.1.22-32

Abstract

ABSTRACT  β-actin is a prominent protein in the immune system. An outcome from gene transcription, the protein protects against pathogen infection, such as receptor clustering, antigen internalization, and regulating vesicle for antigen processing. ß - actin expression will determine the success of immune response of the organism. This study aimed to understand the role of pigment-protein fraction (PPF) from Nannochloropsis oculata in the increase of β-actin expression in humpback grouper Cromileptes altivelis infected by viral nervous necrosis (VNN). The experiment was performed with one negative control (A: normal fish) and three PPF treatments (B: fish + PPF, C: fish + VNN, and D: fish + PPF + VNN). PPF was applied through the sonde method and analyzed with immunohistochemistry technique and Immunoratio software. The results showed that PPF was able to increase β-actin expression on all treatments: A (34.9%), B (38.1%), C (39.1%), and D (51.6%). It demonstrated PPF ability to induce the increase of β-actin expression indicating an improved defense of humpback grouper C. altivelis against VNN infection. Keywords: β-actin, pigment-protein fraction, Nannochloropsis oculata, VNN  ABSTRAK β-aktin adalah protein yang berfungsi penting sebagai salah satu sistem pertahanan tubuh. Protein ini merupakan hasil transkripsi dari gen yang memiliki fungsi penting dalam sistem pertahanan tubuh terhadap infeksi patogen, seperti kelompok reseptor, internalisasi antigen, dan mengatur keluar masuknya vesikel untuk pemrosesan antigen. Ekpresi β-aktin menjadi penentu berhasil atau tidaknya respons imun pada tubuh organisme. Penelitian ini bertujuan untuk mengetahui peran fraksi protein pigment (FPP) dari Nannochloropsis oculata dalam meningkatkan respons β-aktin pada ikan kerapu tikus Cromileptes altivelis yang terinfeksi viral nervous necrosis (VNN). Pengamatan dilakukan pada empat percobaan yaitu, satu kontrol (A : ikan normal) dan tiga perlakuan FPP (B : ikan + FPP, C: ikan + VNN, dan D: ikan + VNN + FPP). Pemberian FPP menggunakan metode sonde dan analisis hasil penelitian menggunakan teknik imunohistokimia dan Immunoratio. Hasil penelitian ini menunjukkan bahwa pengiduksian FPP mampu meningkatkan ekpresi β-aktin yaitu A (34,9%), B (38,1%), C (39,1%), dan D (51,6%). Hal tersebut menunjukan FPP mampu menjadi inducer yang baik untuk peningkatan ekpresi β-aktin. Meningkatnya ekspresi β-aktin bisa dijadikan sebagai indikator pertahanan tubuh ikan kerapu tikus C. altivelis terhadap infeksi VNN. Kata kunci: β-aktin, fraksi protein pigmen, Nannochloropsis oculata, VNN
TECHNIQUE FOR BREDDING CATFISH (CLARIAS GARIEPINUS) AT THE FISHERIES TRAINING AND EXTENSION CENTER (BPPPP) BANYUWANGI Kurdowi, Sahrul Afthoni; Khumaidi, Achmad; Wafi, Abdul
Jurnal Perikanan Unram Vol 15 No 3 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i3.1634

Abstract

Optimal Stocking Density Cultivating Catfish (Clarias gariepinus) in Round Pond Media on a Household Scale Khumaidi, Achmad; Muqsith, Abdul; Wafi, Abdul; Nur Aisyah Jamil, Siti
Journal of Aquaculture and Fish Health Vol. 14 No. 2 (2025): JAFH Vol. 14 No. 2 June 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i2.65654

Abstract

Increasing stocking density in catfish cultivation is part of efforts to increase production to meet catfish needs nationally and internationally. The obstacles faced by household scale cultivators when applying high stocking densities are poor water quality, high feed conversion, slow growth and high mortality. Many novice cultivators on a household scale are tempted by high production results by implementing very high stocking densities, but do not yet understand the cultivation techniques well. This research aims to determine the optimal stocking density for cultivating catfish on a household scale using round tarpaulin ponds. The study used a completely randomized design of four treatments with three replications (250 fish/m3, 500 fish/m3, 750 fish/m3 and 1000 fish/m3). The cultivation container used is a tarpaulin pool with a diameter of 3 meters, and the fish are given commercial feed with 28-33% protein. Production results are also analyzed using the business income analyst model. A stocking density of 500 fish/m3 produces the most optimal production values, namely absolute growth of 4.28 ± 0.02%/day, absolute length growth of 12.4 ± 0.9 cm, survival of 85.97 ± 0.56%, feed conversion of 0.95 ± 0.00, and production of 30.4 ± 0.57 kg/m3 with operating income of Rp. 1,258,967,-. The results of the analysis of production and income values show that treatment with a density of 500 fish/m3 is the best stocking density for cultivating catfish on a household scale using tarpaulin ponds.
SMART ENERGY UNTUK MENINGKATKAN PRODUKSI PADA BUDIDAYA LELE DI POKDAKAN BATU ALAM BERKAH KABUPATEN SITUBONDO Creani Handayani; Ach. Khumaidi; Anita Diah Pahlewi; Gilang Saputra; Alfiyah Emeliya
INTEGRITAS : Jurnal Pengabdian Vol 9 No 2 (2025): AGUSTUS - DESEMBER
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat - Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/integritas.v9i2.7080

Abstract

POKDAKAN Batu Alam Berkah di Kabupaten Situbondo menghadapi kendala pasokan listrik akibat seringnya pemadaman, disertai meningkatnya biaya operasional, serta belum adanya diversifikasi produk olahan lele. Tujuan dari pengabdian ini meningkatkan efisiensi produksi melalui penerapan Smart Energy berbasis tenaga surya dan mendorong pengembangan produk olahan lele bernilai tambah. Metode pelaksanaannya meliputi identifikasi permasalahan, pemasangan sistem Smart Energy, pelatihan penggunaan dan perawatan alat, pelatihan pengolahan lele menjadi nugget dan kerupuk, pelatihan pemasaran serta evaluasi. Hasil pengabdian menunjukkan sistem Smart Energy berfungsi optimal dengan penghematan biaya listrik mencapai 70% per bulan. Mitra berhasil memproduksi dua produk olahan yang dipasarkan secara lokal, serta mengalami peningkatan pengetahuan dan keterampilan dalam pengolahan dan pemasaran. Program ini dinilai berhasil meningkatkan efisiensi energi, diversifikasi produk, dan kapasitas usaha mitra secara berkelanjutan.
Insilico Prediction of Biological Activity of Secondary Metabolites of Amphora sp.: for Development of Natural Medicines Ach. Khumaidi; Abdul Wafi; Abdul Muqsith
Journal of Aquaculture Science Vol 6 No 1IS (1): Vol 6 Issue Spesial 2021 Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v6i1IS.163

Abstract

Diatom Amphora sp. is a type of aquaculture product that has various types of metabolites with very good potential to be used as basic ingredients for drugs or other health products. Insilico analysis of extracts of Amphora sp. was carried out to predict biological activity that can be used as a basis for developing the use of aquaculture products. The research was conducted by extracting Amphora sp. using ethanol solvent with sonication method, the extraction results were analyzed by LC-HRMS, and the metabolite readings were then analyzed for drug potential and biological activity online using swissADME, Protox II, and Way2Drug applications. The results of the LC-HRMS analysis showed that the extracted Amphora sp. showed the highest abundance of valine compounds with 17.6% as well as other compounds L-Norleucin, stearamide, palmitoleic acid, isotretinoin, and arachidonic acid. The results of the ADME/T analysis showed that the six compounds of Amphora sp. extract had quite good potential according to the Lipinski rule of five. Biological activity also shows potential as an anti-inflammatory, antifungal, antibacterial, antiviral, and antineoplastic agent with Pa values ranging from 0.260 to 0.873. These results indicate that the production development of Amphora sp. and its use is very wide, including in the health sector. Keyword: ADME/T, PASSonline, Amphora sp., sonication, aquaculture
Identification of Causes of Mass Death Of Gurami Fish (Osphronemus gouramy) In Gurami Fish Cultivation Sentra, Desa Beji, Kedung Banteng District, Banyumas District, Central Java Achmad Khumaidi; Aris Hidayat
Journal of Aquaculture Science Vol 3 No 2 (2018): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v3i2.53

Abstract

Kasus kematian massal ikan Gurami di Sentra Budidaya Ikan Gurami, Desa Beji, Kecamatan Kedung Banteng, Kabupaten Banyumas, Jawa Tengah berdampak pada menurunnya produksi telur dan benih ikan Gurami. Penelitian ini bertujuan untuk mengidentifikasi patogen penyebab kematian pada ikan Gurami. Metode yang digunakan metode survei dengan pengambilan sampel secara acak, kemudian diidentifikasi keberadaan patogen pada sampel ikan Gurami. Hasil identifikasi patogen pada ikan Gurami menunjukkan adanya infeksi Bakteri (Aeromonas hydrophila, Aeromonas sobria), Parasit (Henneguya sp., Trichodina sp., Vorticella sp.) dan Jamur (Aspergillus sp.). Penelitian ini menunjukkan bahwa kematian massal pada ikan Gurami disebabkan oleh serangan bakteri, parasit, dan jamur. Kata Kunci: ikan Gurami, kematian massal, bakteri, parasit, jamur.
Co-Authors Abdul Muqsith Abdul Muqsith Abdul Muqsith Abdul Muqsith Abdul Wafi Abdul Wafi Abdul Wafi, Abdul Achmad Yani Yayan Agoes Soegianto Alfiyah Emeliya Aris Hidayat Aris Hidayat Astik Umiyah Creani Handayani Desy Febrianti Dimas Galang Prakosa E Ernawati, E Endang Wulandari Suryaningtyas Era Insivitawati Faizin, Rohmanul Fikriyah, Amiqatul Gagan Garnawansah Gilang Saputra Heri Ariadi Irawati Mei Widiastuti Ismail Ismail Ismi Jasila Kurdowi, Sahrul Afthoni Moh. Awaludin Adam Muhammad Rivai Musyaffa Rafiqie Nur Aisyah Jamil, Siti Nurliani, Yunita Pahlewi, Anita Diah Prakosa, Dimas Galang Rini Fitriasari Pakaya Talkhis Hikam Undu, Muhammad Chaidir Yanuhar, Uun Yuliana Yuliana