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Isolation and Identification of Hg-Resistant Bacteria as Bioremediator Agents and Their Potential in Reducing Mercury Contamination SORAYA FITRIA NASIR; ANI M. HASAN; ARYATI ABDUL; YULIANA RETNOWATI
Microbiology Indonesia Vol. 15 No. 2 (2021): June 2021
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.694 KB)

This study aims to describe Hg-resistant bacteria in soil contaminated with gold mining waste and its ability to reduce mercury contamination. This research was initiated by taking soil samples at the gold processing plant in Ilangata Village, Anggrek District, North Gorontalo Regency. Then the research was carried out at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences. Mercury analysis was carried out at the Laboratory of Fisheries Product Quality Development and Testing (LPPMHP) Gorontalo Province and bacterial identification was carried out at the HumRC Research Unit, Faculty of Medicine, Hasanuddin University. The study was conducted from July to October 2020. This study used a descriptive method. The parameters observed were the types of Hg resistant bacteria and the ability of the bacteria to reduce mercury contamination. Data were analyzed descriptively. The results showed that there were four bacterial isolates on the soil contaminated with mercury at 4.5 ppm. Two of them could not be resistant to levels of 10 ppm mercury. However, these four isolates had the ability to reduce mercury levels by 99%. Based on the reconstruction of the phylogenetic tree, bacterial isolate 01 has a close relationship with Stenotrophomonas sp. SB67, while bacterial isolate 02 had a close relationship with Enterobacter cloacae strain CM 1 16S, bacterial isolate 03 was identified as Stenotrophomonas maltophilia and bacterial isolate 04 was a bacterium from the genus Bacillus which was closely related to Bacillus albus strain SQ30 16S.

Chemical Analysis of Sea urchin Diademasetosum Gonads Mohamad Haikal K. Djafar; Margaretha Solang; Marini Susanti Hamidun; Aryati Abdul; Syam S. Kumaji; La Nane
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 2 (2023): August 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i2.42994

Sea urchins Diademasetosum can be found in shallow waters. Sea urchin gonads are nutritionally valuable and capable of accumulating heavy metals. This study aims to determine the moisture content, ash, protein, fat, carbohydrate, Pb, Cd metal content and determine the Maximum Tolerable Intake (MTI) of sea urchin D. setosum gonads. Gonad samples were obtained from three coasts of Huangobotu, Botubarani and Kurenai, Kabila Bone Sub-district, Bone Bolango Regency, using a purposive sampling technique. Gonads were analyzed proximate, including moisture, ash, protein, fat and carbohydrate content. Analysis of Pb and Cd levels using an atomic absorption spectrophotometer. The results were analyzed statistically using One-Way ANOVA Test. The results showed that the average moisture, ash, protein, fat, and carbohydrate content of gonads on the coast of Huangobotu, Botubarani, and Kurenai were significantly different (p0.005); moisture content 71.91%, 75.04%, 78.51%; ash content 1.88%, 1.15%, 2.76%; protein content 9.90%, 12.81%, 15.53%; fat content 7.33%, 3.05%, 4.84%; carbohydrate content 2.35%, 7.92%, 4.93%. Pb levels were significantly different (p0.005) 0.063 mg/kg, 0.039 mg/kg, and 0.018 mg/kg, respectively; Cd levels were not significantly different (p0.005) 0.020 mg/kg, 0.012 mg/kg, 0.014 mg/kg respectively. MTI Pb adults 23.8kg, 38.4kg, 83.3kg, children 5.9kg, 9.6kg, 20.8kg. MTI Cd adults 21kg, 35kg, 30kg, children 5.25kg, 8.75kg, 7.5kg. D. setosumhas potential as a source of protein and can be utilized as food by considering the MTI value.

Analysis of Student Learning Difficulties in the Material of Inheritance of Traits Herinda Mardin; Aryati Abdul; Anita I. Sado
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.3473

This study aims to describe student learning difficulties in inheritance material in class XII SMA Negeri 1 Tapa. This type of research is descriptive quantitative. Data collection techniques used teacher activity sheets, student activity sheets, learning outcomes tests, questionnaires and interviews. The results of the study found: The results of learning implementation at meetings 1 and 2 with a value of 82, 81%, with a good category, at meetings 3 and 4 with a value of 78, 78 with a sufficient category. The results of student activity at meetings 1 to meeting 4 with a value of 54, 17, 51.17, 51.69, 53.39% with a less category. The results of student responses with aspects of physiology and psychology with a value of 69.58, 60.24% with a sufficient category and aspects of sociology with a value of 55.47% with a very sufficient percentage. The results of the analysis of learning outcomes tests with a value of 96% incomplete. In conclusion, there are many students who experience learning difficulties in terms of physiological, psychological, and sociological aspects.

Keterampilan Proses Sains Peserta Didik Melalui Praktikum Pada Materi Sel Kelas Xi Sma Negeri 1 Tapa Ilvayani; Lilan Dama; Aryati Abdul
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4061

This study aims to determine students' science process skills through practicum method on cell material for class XI IPA of SMA Negeri 1 Tapa. The research method used quasi-experimental, a posttest-only non-equivalent control group design. Data collection was performed through observation sheets and test questions. The data analysis used is the t-test. According to research conducted in class XI IPA 1 using the practicum method, students in the experimental class had an average science process skill score of 79%. In contrast, students in the control class, which did not use the practicum method, had an average of 55%. Therefore, the science process skills of students in the experimental class are higher than those in the control class due to the use of the practicum method. Teachers guide students in activities during the learning process by asking open-ended questions that spark a discussion. Students are therefore required to be active, creative, and able to collaborate during the learning process to meet the learning objectives and understand the concepts being taught easily.

Effectiveness of Tembelekan Leaf Extract (Lantana camara L.) on Mortality of Riptortus linearis Rulan U. Mustapa; Chairunnisah J. Lamangantjo; Aryati Abdul; Jusna Ahmad; Wirnangsi D. Uno; Yuliana Retnowati
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4086

This study aims to determine the effect, effective concentration, Lethal Concentration 50 (LC50), and Lethal Time (LT50) of tembelekan leaf extract (Lantana camara L.) on the mortality of Riptortus linearis and metabolic compounds of L. camara leaves. The method was experimental. The study used a completely randomized design (CRD) with five treatments, namely 0%, 10%, 15%, 20%, and 25%, and five replications. Each treatment contained 10 R. linearis test animals. The results of the ANOVA analysis showed the effect of treatment on the mortality of R. linearis (p<0.05). The concentration that showed the highest mortality was 25%. Based on the LC50 test, the 10.155% L. camara leaf extract concentration can kill R. linearis in more than 50% of each treatment. And based on LT50 with a concentration of 25%, it takes 7.681 hours to kill 50% of R. linearis. In L. camara leaf extract contains metabolite compounds, namely alkaloids with a level of 307.87 mg/L, then tannins with a group of 2.75 %b/v, then saponins with a group of 0.31 %b/v, and flavonoids with a group of 1.40 %b/v., It can be concluded that there is an influence of giving tembelekan (Lantana camara L) leaf juice on the mortality of Riptortus linearis.

Effectiveness of Tembelekan Leaf Extract (Lantana camara L.) on Mortality of Riptortus linearis Rulan U. Mustapa; Chairunnisah J. Lamangantjo; Aryati Abdul; Jusna Ahmad; Wirnangsi D. Uno; Yuliana Retnowati
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4086

This study aims to determine the effect, effective concentration, Lethal Concentration 50 (LC50), and Lethal Time (LT50) of tembelekan leaf extract (Lantana camara L.) on the mortality of Riptortus linearis and metabolic compounds of L. camara leaves. The method was experimental. The study used a completely randomized design (CRD) with five treatments, namely 0%, 10%, 15%, 20%, and 25%, and five replications. Each treatment contained 10 R. linearis test animals. The results of the ANOVA analysis showed the effect of treatment on the mortality of R. linearis (p<0.05). The concentration that showed the highest mortality was 25%. Based on the LC50 test, the 10.155% L. camara leaf extract concentration can kill R. linearis in more than 50% of each treatment. And based on LT50 with a concentration of 25%, it takes 7.681 hours to kill 50% of R. linearis. In L. camara leaf extract contains metabolite compounds, namely alkaloids with a level of 307.87 mg/L, then tannins with a group of 2.75 %b/v, then saponins with a group of 0.31 %b/v, and flavonoids with a group of 1.40 %b/v., It can be concluded that there is an influence of giving tembelekan (Lantana camara L) leaf juice on the mortality of Riptortus linearis.

Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort Nur'ain Marton Angio; Aryati Abdul; Syam S. Kumaji; Wirnangsi Din Uno; Yuliana Retnowati; Magfirahtul Jannah
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5506

The hot spring of Pentadio Resort is one of the geothermal areas likely to be the place of bacteria or microorganisms that can produce amylolytic enzymes. This research aims to analyze bacteria in the Pentadio Resort hot springs that possess the potential to produce amylase enzymes, analyze the characteristics of bacterial isolates that produce amylase enzymes in Pentadio Resort hot springs and determine the types of bacteria that produce amylase enzymes in Pentadio Resort hot springs. The research employs a qualitative and descriptive method. The research involved several methods, including sample collection, media preparation, isolation and selection of thermophilic bacterial isolates, purification of thermophilic bacterial isolates, selection of isolates producing amylase enzymes, identification of selected isolates (motility test, biochemical test, gram staining), and molecular identification. The research yielded seven isolates, and isolate code A6 demonstrated the potential to produce amylase enzymes with a clear zone size of 23,26 mm, bacillus, gram positive, and positive motility. Subsequently, molecular identifidation was carried out in which, based on the results of phylogenetic tree reconstractiopisalate A6 was related to the bacteria Bacillus cereus strain MD 152, which belongs to the Bacillus cereus bacteria

P Penerapan Model Pembelajaran Kooperatif Number Head Together untuk Peningkatan Pengetahuan Litrasi Sains Siswa pada Materi Metabolisme Fransiska Radjak; Frida Maryati Yusuf; Aryati Abdul
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.2715

This research was conducted in class XII IPA 1 SMA N 1 Suwawa with the aim of knowing the literacy knowledge of students through the application of the Numbered Head Together cooperative learning model. The model used in this research is the Classroom Action Research Kemmis and Tagart models using 3 cycles, with research stages namely a) Planning, b) Implementation or action, c) Observation, d) Reflection. Based on the analytical instruments and analysis of scientific literacy-based learning outcomes, it can be seen that the NHT type cooperative learning model can increase students' scientific literacy knowledge which includes 3 indicators including scientific question assistance, explaining phenomena scientifically, and using scientific evidence , this is evidenced by improvements in the activities of teachers and students, as well as the improvement of learning outcomes achieved. The application of the NHT learning model can increase students' scientific literacy knowledge, because the application of the NHT learning model can help train and increase students' scientific literacy knowledge, especially in metabolic materials. The characteristics of metabolic substances which generally require more understanding can be complemented by the application of the NHT learning model which precipitates cooperation and sharing between peers which also requires responsibility for oneself due to random calling of noor. The advantages of the NHT learning model are that it can improve student achievement, are able to deepen student understanding, train student responsibility, delight students in learning, develop student curiosity and increase self-confidence also help increase students' scientific literacy knowledge because it involves students with their learning environment, and thinking activities together

Analysis of Student Learning Difficulties in the Material of Inheritance of Traits Herinda Mardin; Aryati Abdul; Anita I. Sado
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.3473

This study aims to describe student learning difficulties in inheritance material in class XII SMA Negeri 1 Tapa. This type of research is descriptive quantitative. Data collection techniques used teacher activity sheets, student activity sheets, learning outcomes tests, questionnaires and interviews. The results of the study found: The results of learning implementation at meetings 1 and 2 with a value of 82, 81%, with a good category, at meetings 3 and 4 with a value of 78, 78 with a sufficient category. The results of student activity at meetings 1 to meeting 4 with a value of 54, 17, 51.17, 51.69, 53.39% with a less category. The results of student responses with aspects of physiology and psychology with a value of 69.58, 60.24% with a sufficient category and aspects of sociology with a value of 55.47% with a very sufficient percentage. The results of the analysis of learning outcomes tests with a value of 96% incomplete. In conclusion, there are many students who experience learning difficulties in terms of physiological, psychological, and sociological aspects.

Keterampilan Proses Sains Peserta Didik Melalui Praktikum Pada Materi Sel Kelas Xi Sma Negeri 1 Tapa Ilvayani; Lilan Dama; Aryati Abdul
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4061

This study aims to determine students' science process skills through practicum method on cell material for class XI IPA of SMA Negeri 1 Tapa. The research method used quasi-experimental, a posttest-only non-equivalent control group design. Data collection was performed through observation sheets and test questions. The data analysis used is the t-test. According to research conducted in class XI IPA 1 using the practicum method, students in the experimental class had an average science process skill score of 79%. In contrast, students in the control class, which did not use the practicum method, had an average of 55%. Therefore, the science process skills of students in the experimental class are higher than those in the control class due to the use of the practicum method. Teachers guide students in activities during the learning process by asking open-ended questions that spark a discussion. Students are therefore required to be active, creative, and able to collaborate during the learning process to meet the learning objectives and understand the concepts being taught easily.

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