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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) International Journal of Biosciences and Biotechnology Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES The Journal of Pure and Applied Chemistry Research Indonesian Journal of Chemistry Journal of Health Sciences and Medicine

Ketut Ratnayani

Program Studi Kimia, Fakultas MIPA Universitas Udayana, Bali-Indonesia

Author-ID : 2921952

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Electrical & Electronics Engineering Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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SKRINING POTENSI JENIS BIJI POLONG-POLONGAN (Famili Fabaceae) DAN BIJI LABU-LABUAN (Famili Cucurbitaceae) SEBAGAI KOAGULAN ALAMI PENGGANTI TAWAS Ni Komang Ariati; Ketut - Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 11. No. 1 Januari 2017
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Telah dilakukan penelitian tentang Skrining Potensi Beberapa Jenis Biji Polong-polongan (Famili Fabaceae) dan Biji Labu-labuan (Famili Cucurbitaceae) Sebagai Kuagulan Alami Pengganti Tawas. Potensi biji-bijian ditentukan dengan metode turbidimetri dengan menghitung persentase penurunan turbiditas dari masing-masing biji-biji tersebut yang diujikan pada sampel air. Hasil Penelitian menunjukkan bahwa serbuk biji asam jawa dari suku polong-polongan terbukti mampu berperan sebagai kuagulan alami, sedangkan biji bari suku labu-labuan kurang potensial menurunkan kekeruhan air. Persen penurunan kekeruhan dengan menggunakan kuagulan biji dari suku polong-polongan (lamtoro, merak, asam jawa) berturut-turut 32,35%, 39,99%, 62,75% dan biji dari suku labu-labuan (labu kuning, melon, semangka) berturut-turut 47,50%, 44,71%, 51,81%.

ANALISIS ASAM AMINO PENSTIMULASI SEKRESI INSULIN DALAM BIJI KECIPIR, BIJI ASAM, DAN BIJI KELOR DENGAN HPLC Dita Rizkiyanti; Ni Made Suaniti; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Seeds are a source of high protein when compared with other parts of the plant. Compared to soy bean, the use of winged bean seeds, tamarind seeds, moringa seeds as protein sources are still very limited. Protein composed of several amino acids bond together to form a polypeptide. Some amino acids have been investigated to act as stimulating insulin secretion, namely, arginine, alanine, phenylalanine, isoleucine, and lysine. The aim of this study was to determine the potential content of amino acids stimulating the secretion of insulin in winged bean seeds, tamarind seeds, and moringa seeds. Based on the total content of amino acids in each seeds, the results showed that moringa seeds have the highest levels of total amino acids stimulating insulin secretion (16.4%), followed by winged bean seeds (16.2%), and tamarind seeds (12.1%). But if seen by the levels of each amino acid, the winged bean seeds on average had the highest amino acid content. The highest levels of arginine, alanine, phenylalanine, isoleucine, and leucine were found in winged bean seeds, while only one specific amino acid i.e. lysine was found to be the highest level on moringa seeds. It can be concluded that the most potential seeds as a source of amino acids stimulating insulin was the winged bean seeds, that will be useful in the prevention or treatment of diabetes mellitus.

AKTIVITAS ANTIRADIKAL BEBAS SERTA KADAR BETA KAROTEN PADA MADU RANDU (Ceiba pentandra) DAN MADU KELENGKENG (Nephelium longata L.) I M. Oka Adi Parwata; K. Ratnayani; Ana Listya
Jurnal Kimia (Journal of Chemistry) Vol. 4, No. 1 Januari 2010
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.94 KB)

It has been done the determination of free antiradical activity and beta caroten concentration on randu honey(Ceiba pentandra) and kelengkeng honey (Nephelium longata L.). The free antiradical activity determined bydiphenyl pykril hydrazil (DPPH) method according to sphectrophotometry UV-Vis, before that the honey was dilutedwith methanol and then the absorbance was measured on 497nm, 517 nm, and 537 nm. The beta karotenconcentration determination was done TLC-Sphetrophotodensitometrically by using beta caroten standard; the honeywhich was resulted by methanol maseration was partiated with acetone p.a. The next step was done by TLC withethyl acetate as an eluent : chloroform (3:7) and the peak of the analyt was read on TLC scanner 3.The result showed that the free antiradical activity on kelengkeng honey was greater than randu honey.Whereas the beta caroten concentration on kelengkeng honey was fewer than beta caroten concentration randuhoney. The activity of free antiradical and beta caroten concentration was 82,10 % and 1,9687 mg/100 g respectively,whereas for randu honey was 69,37 % and 3,6327 mg/100 g respectively.

PERBANDINGAN AKTIVITAS SPESIFIK EKSTRAK KASAR ENZIM BROMELIN BUAH NANAS YANG DIISOLASI DENGAN BEBERAPA JENIS GARAM PENGENDAP I. A. Preharsini Kusuma; A. A. I. A. M. Laksmiwati; Made Arsa; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The aim of this research was to find specific activity of bromelain enzyme extracted from crude extract of pineapple fruit (Queen variety) by salting out method using three different salts, which were (NH4)2SO4, NaCl, and Kusamba traditional salt. Crude extract of bromelain enzyme was obtained by precipitation method using salt with different levels of saturation. This study included determination of total protein concentration by using Biuret method and protease activity by using Anson method to get the specific activities of the crude extract. The results showed that the highest specific activities of bromelain enzymes precipitated with the three salts (NH4)2SO4, NaCl and Kusamba traditional salt with the ratio of 1: 0.94: 0.56 were 0.2887 U/mg (at 20% saturation) ; 0.2705 U/mg (at 40% saturation) and 0.1623 U/mg (at 80% saturation). Fractination of enzyme with the NaCl and Kusamba traditional salt gave smaller specific activity value than (NH4)2SO4. This results may caused of NaCl had smaller ionic strength, the presence of other ions in Kusamba traditional salt and the different solubility of bromelain enzyme in different salts. However, due to the ratio of NaCl approached (NH4)2SO4 and the availability of Kusamba traditional salt they could be used as alternative salts to replace (NH4)2SO4 in fractination bromelain enzyme from crude extract of pineapple fruit

NILAI DAYA HANTAR LISTRIK, KADAR ABU, NATRIUM, DAN KALIUM PADA MADU BERMERK DI PASARAN DIBANDINGKAN DENGAN MADU ALAMI (LOKAL) Putu Setya Sri Antary; Ketut Ratnayani; A. A. I. A. Mayun Laksmiwati
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 2 Juli 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Honey sweet substance naturally produced by bees. Good honey must meet the requiremends setby the Indonesian National Standard (SNI). The aim of this research was to determine thequality of some branded honey samples sold in some markets around Denpasar and natural honey (local) collected from beekeepersby examining their electrical conductivities, ash contents, and ratio of potassium to sodium. The electrical conductivities were determinedby applying the standard procedures established by IHC (International Honey Commission), ash contents were quartified by following the procedures of standard SNI01-3545-2004, while the determination of potassium and sodium contents were carried out by the use of Atomic Absorption Spectrophotometry. The results showed that the electrical conductivities and ash contentsin all honey samples studiedmet the maximum limits of standard values which were of 0.8 mS.cm-1 and 0.50 %, respectively. The results also demonstrated that the value of electrical conductivities were proportional to the ash contents. The ratio of sodium to potassium of a good quality honeyis approximately 4 : 1. In this research, it was found that the ratio of Na : K for samples A (natural honey), B, C, and D, were of (3 : 1), (2: 1), (4 : 1), and (4 : 1), respectively, while for sample E was of 8 : 1. It was concluded that A, B, C, and D samples meet the criteria of good quality honey, while sample E (not branded honey) didnot meet the criteria.

KADAR THIAMIN HIDROKLORIDA (VITAMIN B1) PADA NASI BERAS PUTIH DAN BERAS MERAH PADA BERBAGAI WAKTU PENYIMPANAN PADA ALAT MAGIC-COM A. A. I. A. Mayun Laksmiwati; Ketut Ratnayani; Ni Wayan Agustini
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 1 Januari 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.08 KB)

This paper discusses the influence of storage time on the thiamine hydrochloride content in rice. White rice (C36) and red rice were stored in a magic-com for a period of time, of 0 hours, 6 hours and 12 hours. Thiamine hydrochloride contents in rice were separated with the use of a chromatography column with alumina oxide, while and the measurement was carried out by UV-Vis spectrophotometry.It was found that the thiamine hydrochloride content in red rice stored within 0 hours, 6 hours and 12 hours were, 5.3 mg/kg, 4.5 mg/kg and 2.8 mg/kg, respectively, while in white rice they were found to be 2.6 mg/kg, 2.0 mg/kg and 1.4 mg/kg, respectively.

ISOLASI DNA METAGENOMIK DARI TANAH HUTAN MANGROVE PANTAI SUWUNG BALI I Nengah Wirajana; Darma Asih Yuliana; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 1 Januari 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Metagenomic DNA isolation from mangrove forest soils of Suwung Beach-Bali was conducted to exploit the biocatalytic potential of microbial communities for the discovery of novel cellulase. The whole DNA isolation was conducted by modification of preparation step by Marco (2010) and DNA extraction step by Amorim et al (2008). The results of metagenomic DNA isolation were analyzed by spectrophotometry and agarose gel electrophoresis. The results showed that whole DNA was able be isolated successfully, but protein and humic acid were found as contaminant.

UJI AKTIVITAS PROTEASE GETAH LABU SIAM DAN TALAS SERTA PERBANDINGANNYA TERHADAP GETAH PEPAYA Ketut Ratnayani; A. A. Ayu Septri Juwarni; A. A. I. A. Mayun Laksmiwati; I G. A. Kunti Sri Panca Dewi
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Papaya (Carica papaya L.) latex has been used commercially as a protease (papain) sources. However it can cause itchy on the skin. Some other latex plants that can cause itchy were taro and chayote. They were strongly predicted to have protease components. This research aimed to determine protease activity of plants latex of taro (Xantosoma sagittifolium (L.) Schott and chayote (Sechium edule (Jacq.) Sw.). The protease activity of these latex then were compared to the protease activity of papaya latex. Latex of papaya and taro were collected from fruit, while latex of chayote was collected from corm. The assay of protease activity was based on spectrofotomeric methods (Anson’s method) using casein as substrat. The result of protease activity assay showed that taro and chayote latex positively contains protease but their protease activities were less than papaya latex which were 0.0123 U/mL and 0.0264 U/mL respectively. It can be concluded that taro and chayote latex were potentially used as alternative protease sources. The protease activity ratio of taro latex to papaya was 1: 74.75 and the ratio of chayote latex to papaya latex was 1: 34.82.

SKRINING SELULASE DARI TANAH HUTAN MANGROVE PANTAI SUWUNG BALI I Nengah Wirajana; Ketut Ratnayani; Darma Asih Yuliana
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 2 Juli 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.478 KB)

Mangrove soil has high biodiversity and well known as potential location for enzymes exploration. The aim of direct screening for cellulase of mangrove soil is to find out the cellulase activity from mangrove soil. Mangrove soils were collected from three different locations labelled as A (8o43’38.20”SL), B (8o43’46,18”SL), and C (8o43’37,38”SL). The screening was conducted by Filter Paper Assay and Carboxymethyl Cellulose Assay methods. The results showed that cellulase activities can be measured directly from mangrove soil samples of Suwung Beach-Bali. The highest cellulase activities were 0,866 U/g soil by Filter Paper Assay and4,176 ± 0,630 U/g soil by Carboxymethyl Cellulose Assay, given by soil samples C.

AKTIVITAS PROTEASE PADA GETAH BAGIAN BATANG DARI TIGA JENIS SPESIES TANAMAN KAMBOJA (Plumeria L) K. Ratnayani; M. Nazib; J. Sibarani; A.A.I.A M. Laksmiwati
Jurnal Kimia (Journal of Chemistry) Vol.12 No.2 Juli 2018
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Penelitian ini bertujuan untuk melakukan uji aktivitas protease pada getah bagian batang dari tiga spesies tanaman kamboja yaitu Plumeria rubra L, Plumeria obtusa L dan Plumeria pudica Jacq yang banyak dibudidayakan di wilayah Bali. Pengujian aktivitas protease dilakukan secara spektrofotometri (metode Kurnitz termodifikasi) menggunakan kasein sebagai substrat. Hasil penentuan aktivitas protease pada tiga spesies tanaman kamboja menunjukkan bahwa spesies Plumeria rubra L. memiliki aktivitas protease rata-rata relatif lebih tinggi yaitu 0,0398 ± 0,00034 U/mL, disusul oleh spesies Plumeria pudica Jacq. yaitu 0,0371 ± 0,00019 U/mL, dan Plumeria obtusa L. yaitu 0,0365 ± 0,00032 U/mL. Spesies Plumeria rubra L. merupakan jenis tanaman kamboja yang paling umum, memiliki paling banyak varietas dan paling banyak dibudidayakan di wilayah Bali dibandingkan spesies lainnya. Dari hasil penelitian ini dapat diketahui bahwa jenis ini berpotensi sebagai sumber protease alternatif. Kata kunci: aktivitas protease, getah kamboja, tiga spesies

Co-Authors A. A. Ayu Septri Juwarni A. W. Dwiputri Acintya, N. M. T. C. Amanda Awalia Ramadhani Ana Listya Anak Agung Istri Agung Mayun Laksmiwati Ariati, N. K. Darma Asih Yuliana Deniariasih, N.W. Dewi, I G. A. K. S. P. Dita Rizkiyanti Helen Helda Prastika I G. A. M. A. S. Gitadewi I Gusti Ayu Kunti Sri Panca Dewi I Ketut Suter I Made Oka Adi Parwata I Nengah Kencana Putra I Nengah Wirajana I Wayan Sudiarta I Wayan Suirta I. A. Preharsini Kusuma I. A. R. Astiti Asih Ida Bagus Swardana Indriani Wisnu Susanto Panjaitan James Sibarani Lia Kusumaningrum Liangky Syane S Listiyanti, N. K. L. M. A. Pratiwi M. Nazib Made Arsa Maman Sudiarto Ni G. A. M. Dwi Adhi Suastuti Ni Komang Ariati Ni Luh Putu Mega Wahyuni Ni Made Puspawati Ni Made Suaniti Ni P. Indah Septian P. Ni Putu Frida Oktaningtias Widiarthi Ni Putu Wiwik Oktayuni Ni Wayan Agustini Ni Wayan Wisaniyasa NYOMAN SEMADI ANTARA Putra, A. A. B. Putu Eka Purnama Putu Lita Astriani Putu Setya Sri Antary Ro’yal Aini Sagun Chandra Yowani Suarsa, I W. Syamsul Arifin Tutut Hardikawati

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