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All Journal METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Biogenesis: Jurnal Ilmiah Biologi Metamorfosa: Journal of Biological Science Open Access DRIVERset
MADE SUSUN PARWANAYONI
Jurusan Biologi, FMIPA, Universitas Udayana, Denpasar, Bali.
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology

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Journal : METAMORFOSA Journal of Biological Sciences

 1 
Uji Antioksidan Ekstrak Daun Tanaman Leunca (Solanum nigrum L.) Affrina Fauziah; Sang Ketut Sudirga; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p03

Abstract

Leunca plant (Solanum nigrum L.) is one of the plants that is often used as food for the people of Indonesia. Leunca plant leaves are widely used for herbal medicines. This study aims to determine the phytochemical content and antioxidant activity of leunca leaf extract (Solanum nigrum L.). The study was conducted at the Analytical Laboratory of Udayana University, the Organic Laboratory of Chemistry Study Programs at Udayana University and the Laboratory of the Faculty of Agriculture of Udayana University. Phytochemical tests were carried out to determine the presence of alkaloid compounds, steroids / triterpenoids, flavonoids, phenols and tannins. The antioxidant activity of leunca leaf extract (Solanum nigrum Linn) was tested by DPPH method (1-1-diphenyl-2-picrilhidrazil), with 5 different concentrations and consisted of 4 replications. Leunca positive leaf extract contains alkaloids, steroids / triterpenoids, flavonoids, phenols and tannins. The results showed that dried leunca leaf extract and fresh leunca leaf extract had different antioxidant activity. Dry leunca leaf extract is classified as strong antioxidant with antioxidant activity value of 64.40%, IC50 value of 95.12 ppm and fresh leunca leaf extract is classified as weak antioxidant with antioxidant activity value of 16.83%, IC50 value of 243.66 ppm. Keywords: Solanum nigrum L., herb, phytochemicals, antioxidant, DPPH
Identifikasi Jamur Endofit pada Tanaman Anggur Bali (Vitis vinifera L. Var Alphonso lavalle) serta Potensi Antagonisnya terhadap Botrytis cinerea Pers. Penyebab Penyakit Busuk Kelabu Putu Anggan Pradipta Utama; Meitini Proborini Wahyuni; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p01

Abstract

This research aims to isolate and test the ability of endophytic fungi from the Bali Grape plant (Vitis vinifera L. var Alphonso lavalle) against botrytis cinerea pers. cause of gray rot in Grapes Bali. This research is an exploration study that is then continued with experimental. This research sample was isolated from Balinese grapes, namely healthy roots, stems, and leaves as well as diseased fruit parts from Balinese vineyards in Banyupoh Village, Gerokgak, Buleleng, Bali. Isolates of endophyte fungi that are successfully isolated are then tested using dual culture methods against pathogenic fungi. The test results of five isolates of endophytic fungi obtained three types of fungi that have the tasteless power of two types of fungi Trichoderma spp. (1) (2) and Mucor spp. with consecutive bonding power of 90.67 ± 16.17%, 75.67 ± 31.13%, and 82.67 ± 39.02. These results show that endophyte fungi have potential as biocontrol agents. Keywords : Mushroom endophyte, Botrytis cinerea Pers., Biocontrol
Elusidasi Dan Daya Hambat Ekstrak Etanol Daun Sembung Delan (Sphaeranthus indicus L.) Terhadap Candida albicans ATCC 1023 Yulita Salma Lani; Ida Bagus Gde Darmayasa; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p18

Abstract

The sembung delan (Sphaeranthus indicus L.) plant is one of the weeds in Indonesia which has great potential as an alternative antifungal. The purpose of this study was to determine the ability of sembung delan leaf extract to inhibit the fungus Candida albicans ATCC 1023, as a pathogenic species and cause of candidiasis, as well as chemical compounds contained in the extract. The research design used in this study was a completely randomized design (CRD) divided into 7 treatments and 4 repetitions with one treatment factor, namely the concentration of 1% (w/v), 2% (w/v), 3% (w/v), 4% (w/v), 5% (w/v). Positive control (Nystatin) and negative control (Solvent). Phytochemical tests were carried out and analysis of compound content using GC-MS. The results showed that sembung delan leaf extract was able to inhibit C. albicans ATCC 1023 with the greatest inhibition, namely at a concentration of 5% (w/v) with a diameter of 13.00 mm, a minimum concentration of 0.3% (w/v) with a diameter of 5.925 mm. Statistically significantly different (P?0.05) with a concentration of 0.1% (w / v), and 0.2% (w / v) with a diameter of 0.000 mm and an optimum concentration of 4% (w/v) with a diameter of 11.25 mm. Phytochemical test results of the crude extract of sembung delan contain saponins, phenols, steroids, terpenoids, alkaloids, and tannins. For GC-MS analysis, 7 active compounds were obtained with 6 of them known as anti-fungal compounds, namely Thymol; 1-Tetradecanamine, N, N-dimethyl-; Benzene, 2-methoxy-1,2,3-trimethyl-; 6-Octenal, 3,7-dimethyl-; Thymol and Benzyl chloride. The active compounds contained in sembung delan leaf extract have the opportunity to be used as herbal ingredients to control C. albicans ATCC 1023. Keywords: Minimum Inhibitory Concentration, phytochemicals, thin layer chromatography, gas chromatography mass spectrometry.
Aktivitas Antibakteri Fungi Endofit dari Rimpang Jahe Gajah (Zingiber officinale var. Roscoe) terhadap Methicillin Resistant Staphylococcus aureus (MRSA) Ni Ketut Yuliana Sari; Retno Kawuri; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p11

Abstract

This study aimed to isolate and test the antibacterial activity of endophytic fungi from elephant ginger rhizome (Zingiber officinale var. Roscoe) against MRSA. This research is an exploratory research which is continued with experimental. The research sample was obtained from three locations. The first sample was from Pancasari Village, Singaraja, Bali. The second sample was from Peguyanganan Village, Denpasar, Bali. The third sample is from Tebuana Village, Tampaksiring, Bali. This research was conducted at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Udayana University. Eight isolates of endophytic fungi were successfully isolated with each inhibitory zone against MRSA, Fusarium sp. 1 (21 mm), Fusarium sp. 2 (19 mm), Fusarium sp. 3 (20 mm), Fusarium sp. 4 (21 mm), Aspergillus sp. 1 (32 mm), Aspergillus sp. 2 (25 mm), Fungi S3F1 (13 mm) and Fusarium sp. 5 (25 mm). This shows that endophytic fungi can be used as an alternative producer of antibacterial compounds. Keywords: Antibacterial, Endophytic fungi, Methicillin Resistant Staphylococcus aureus.
Formulasi Body Mask dari Daun Pegagan (Centella asiatica L.) untuk Perawatan Kulit Stareast Wulandari Sigiro; Ni Luh Arpiwi; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p15

Abstract

Body mask yaitu satu diantara kesediaan kosmetik yang dipakai dalam proses akhir saat melakukan perawatan kulit. Perawatan kulit terkhususnya dalam kulit tubuh membutuhkan kosmetika yang sifatnya menimbulkan lembab. Oleh karena itu perlu dilakukan perawatan agar dapat menjaga kulit supaya selalu bersih serta sehat, salah satunya dengan perawatan kulit yaitu dengan body mask. Body mask memiliki banyak manfaat yaitu memberikan kelembaban, membuat kulit menjadi kencang, memberikan nutrisi pada kulit, membuat lembut kulit, membuat bersih pori-pori dan mencerahkan warna kulit. Satu diantara bahan alam yang bisa dipakai dalam melakukan perawatan kulit yaitu daun pegagan (Centella asiatica L.). Ekstrak pegagan memiliki salah satu senyawa kandungan yaitu asiaticosida yang dapat berkhasiat menstimulasi kolagen pada jaringan kulit. Penelitian yang dilakukan mempunyai tujuan agar dapat mengetahui kandungan fitokimia daun pegagan, mengetahui formulasi sediaan masker bubuk daun pegagan yang baik, serta mengetahui formulasi terbaik menurut probandus. Metode yang dipakai saat melakukan penelitian yang dilaksanakan yaitu uji fitokimia, pembuatan body mask, uji pH, uji organoleptik, dan uji hedonik. Penelitian yang dijalankan ini menghasilkan uji fitokimia golongan steroid, saponin, flavonoid, dan tanin diperoleh hasil positif. Kualitas body mask yang dihasilkan yaitu pH 5,41, dan tidak menimbulkan iritasi kulit. Uji organoleptik tekstur halus, aroma green tea, warna putih kehijauan. Formulasi body mask yang paling disukai oleh probandus adalah menggunakan basis yang ditambah 45 g (F2) bubuk daun pegagan. Kata Kunci : Body mask, daun pegagan (Centella asiatica L.), perawatan kulit.
Co-Authors Affrina Fauziah Deni Agung Priandoko Dewa Ngurah Suprapta Fitria Dwijayanti I Gusti Ayu Diana Meirani I Gusti Ayu Laras Pratama Putri I Gusti Ayu Sugi Wahyuni I Ketut Sundra I WAYAN EKA DHARMAWAN Ida Bagus Gede Darmayasa Joko Wiryatno Made Ria Defiani Meitini Proborini Wahyuni Nasha Hadi Rahmadanty Ni Ketut Yuliana Sari Ni LUH ARPIWI Ni Luh Suriani Ni Made Rai Suarni Ni Made Suartini Nyoman Darsini Putu Anggan Pradipta Utama Rachel Daniella Dinda Maria Lumban Tobing Retno Kawuri SANG KETUT SUDIRGA Stareast Wulandari Sigiro Yulita Salma Lani