Luh Dewi Anggreni
Laboratorium Diagnosis Klinik Dan Patologi Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Denpasar, Bali, Indonesia

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Journal : Jurnal Veteriner

Profil Kimia Klinik Darah Sapi Bali (BLOOD CLINICAL CHEMISTRY PROFILES OF THE BALI CATTLE) Anak Agung Sagung Kendran; I Made Damriyasa; Nyoman Sadra Dharmawan; Ida Bagus Komang Ardana; Luh Dewi Anggreni
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

For the evaluation of clinical tests in veterinary laboratories a reference basis of normal values ofclinically healthy farm animals is essential. It is well known that variables such as breed, age and sex havean influence on many blood parameters. The reference value of blood chemistry of bali cattle is scanty. Theaim of the current study was to evaluate blood chemistry of bali cattle includes alanine aminotransferase(ALT), aspartate aminotransferase (AST), urea, creatinine and glucose, measured by an auto analyzer.These data were used to establish reference intervals in bali cattle of different ages and sex. Blood wascollected from 195 (21 young males, 54 adult males, 60 young females, and 60 adult females) clinicallyhealthy bali cattle by means of jugular venipuncture. Values from different age and sex were comparedstatistically. The results of the study was obtained that ALT and blood glucose significantly higher inmales Bali cattle (P <0.05) then in females. ALT, AST and urea were significantly higher in adults Balicattle (P<0.05) then in young Bali cattle. However, glucose of young Bali cattle was higher when comparedwith the adult. There was no significant difference in the creatinine levels between adults and the youngones. The results of the current study provide reference intervals for clinical chemistry of Bali cattle
Yuniati Kencana TOTAL DAN DIFERENSIAL LEUKOSIT AYAM PETELUR PASCAVAKSINASI NEWCASTLE DISEASE DAN AVIAN INFLUENZA Gusti Ayu Yuniati Kencana; Anak Agung Sagung Kendran; Luh Dewi Anggreni; Ni Wayan Helpina Widyasanti
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.586 KB) | DOI: 10.19087/jveteriner.2018.19.2.190

Abstract

The purpose of this study was to determine total and differential leukocyte of layer after vaccinated with Newcastle Disease (ND) and Avian Influenza (AI) combination vaccine. The research was conducted on commercial layer breeding in Denbatas Village, Tabanan Sub-district, Bali. This study used 30 layer samples. Vaccination was done with ND-AI combination vaccine which was a combination of ND vaccine strain Lasota with AI vaccine subtype H5N1. ND-AI combination vaccine was an inactive vaccine. Chicken was vaccinated at 18 weeks of age before laying eggs. Vaccination was done with one dose (0.5 ml) through thigh muscle injection. Chicken blood sampling was done twice ie, two weeks and three weeks after vaccination. The examination of the total leukocytes was performed by count chamber, whereas the differential leukocyte was examined from a blood smear preparation by Giemsa staining. Data of total and differential leukocyte examination were analyzed by descriptively quantitative. The results concluded that layer has leukopenia and heteropenia which were probably caused by hormonal factors and stress due to blood collection.
Imunitas Protektif Mencit Terhadap Cairan Kista Taenia saginata (PROTECTIVE IMMUNITY OF MICE AGAINST CYST FLUID OF TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; I Made Damriyasa; Ida Bagus Made Oka; Kadek Swastika; Luh Dewi Anggreni; Nyoman Mantik Astawa
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to determine immune response of mice against vaccines derived fromcyst fluid of Taenia saginata. The study was conducted using four BALB/c mice aged 6 weeks as experimentalanimals. All experimental animals were vaccinated intra peritoneal with Taenia saginata cyst fluidemulsified in Freund’s adjuvant. Immune response in the mice was determined by detecting antibodiesusing ELISA and by the presence of lymphocytes through evaluation of blood smear. The results showedthat the cyst fluid of Taenia saginata was antigenic and capable of inducing antibody responses that weredetected by ELISA. Mean antibody titers obtained in the results of the first, second, third, and fourth ofvaccination was 3.3 units; 17.9 units; 21.2 units; and 72.1 units; respectively. Evaluation of blood smear ofvaccinated mice showed an increase in the percentage of lymphocytes after vaccination with an average66.75%, compared with the average of lymphocytes before vaccination which was 40.75%. Further researchis still required in experimental animals by vaccination followed by challenge test with Taenia saginataeggs.
Toksisitas Ekstrak Daun Sirih Merah pada Tikus Putih Penderita Diabetes Melitus (TOXICITY OF RED BETEL EXTRACT IN DIABETIC WHITE RAT ) Anak Agung Sagung Kendran; Ketut Tono Pasek Gelgel; Ni Wayan Linda Pertiwi; Made Suma Anthara; Anak Agung Gde Oka Dharmayuda; Luh Dewi Anggreni
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to study the toxicity of red betel  (Piper crocatum) extract in diabeticwhite rat based on ALT and AST activities. This research used 20 male white rats, which randomlydivided into five groups, P1: given only aqua; P2: given alloxan 120mg/kg bw; P3: given alloxan 120 mg/kgbw and red betel leaf extract 50 mg/kg bw; P4: given alloxan 120 mg/kg bw and red betel leaf extract 100mg/kg bw; P5: given alloxan 120 mg/kg bw and glibenclamide suspension 1 mg/kg bw. ALT and ASTactivities were measured by using reflovet plus Machine. The collected data were analyzed by usinganalysis of covariance. The result showed no significant  effect (P>0.05) was observed on giving red betelleaf extract in diabetic white rat for ALT and AST activities.  It can be concluded that red betel leaf extractis potential for diabetic treatment in white rat  and it is not toxic for the rat’s ALT and AST activities.
Hematologi dan Biokimia Darah Babi yang Diberi Pakan Limbah Ubi Jalar Ungu I Gede Mahardika; Luh Dewi Anggreni; Nyoman Sadra Dharmawan
Jurnal Veteriner Vol 24 No 1 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.1.32

Abstract

Babi adalah spesies hewan yang mudah beradaptasi dan berkembang pesat sehingga banyak dipelihara dan dikembangkan. Kebanyakan peternakan babi di Bali dilakukan secara sederhana dan tradisional. Kondisi ini perlu didukung, diantaranya dengan pemanfaatan pakan yang bersumber dari limbah tanaman. Salah satu limbah tanaman yang dapat dimanfaatkan sebagai bahan pakan alternatif adalah ubi jalar ungu (Ipomoea batatas L). Tujuan penelitian ini untuk memperoleh data hematologi dan biokimia darah babi yang diberi pakan bersumber limbah ubi jalar ungu. Parameter hematologi yang diperiksa meliputi eritrosit, hemoglobin, hematokrit, indeks eritrosit, leukosit dan diferensial leukosit. Pemeriksaan dilakukan menggunakan mesin otomatis Hematology Analyzer Sysmex XS-800i. Pemeriksaan biokimia darah meliputi protein, kolesterol, trigliserida, dan glukosa, menggunakan alat Photometer 5010 V5+. Hasil penelitian menunjukkan bahwa nilai hematologi dan biokimia darah babi pada perlakuan A yaitu kelompok babi kontrol yang diberi pakan standar, tanpa limbah ubi jalar ungu; perlakuan B yaitu kelompok babi yang diberi pakan standar dengan tambahan limbah ubi jalar ungu 7,5%; dan perlakuan C yaitu kelompok babi yang diberi pakan standar dengan tambahan limbah ubi jalar ungu 15%, semuanya masih berada pada rentang rujukan nilai normal. Hal ini membuktikan bahwa pemberian pakan limbah ubi jalar ungu 7,5% dan 15% tidak berpengaruh terhadap parameter hematologi dan biokimia darah yang diukur.