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Modification of Plasmids and Cry Genes of Bacillus thuringiensis subsp. kurstaki HD-I After Treatment With Ethylmethanosulfonate (EMS) and UV Light Jusuf, Eddy
Annales Bogorienses Vol. 9 No. 2 (2004): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bacillus thuringiensis subsp. kurstaki HD-1 is a potential insecticidal bacterium producing five type of a-endotoxin crystal proteins. This bacterium is widely commercialized due to its wide spectrum toxicity against both Lepidopteran and Dipteran larvae. The objective of this work was to create autolysin deficient mutant causing cell fails to lyse. This mutant yield intact cells within spore and a-endotoxin crystal protein protected inside, from which an undamaged active bio-insecticide would be obtained. Two methods of mutagenesis, 2% of ethylmethanosulfonate and 10, 25, and 50 second of UV light exposure, resulted in mot (loss of motility) mutation. Observation showed that 14 of 20 survived mutants have lost some of its plasmids (varied from one to five). while the other six maintained their plasmids. By employing the polymerase chain reaction (PCR) the change on the cry genes was studied.
Electric Vehicle Adoption in Jabodetabek: The Impact of Infrastructure, Cost, and Perceived Value Jusuf, Eddy; Sunitiyoso, Yos
Jurnal Samudra Ekonomi dan Bisnis Vol 17 No 1 (2026): JSEB
Publisher : Fakultas Ekonomi dan Bisnis Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jseb.v17i1.12645

Abstract

This research investigates the primary factors affecting consumers’ intention to adopt electric vehicles (EVs) in the Jabodetabek region of Indonesia. This research analyzed data from 188 participants using a quantitative method through PLS-SEM. The model integrates the Theory of Planned Behavior (TPB), the Perceived Value (PERVAL) framework, and several contextual factors. The results show that perceived quality and price value significantly affect attitudes, while charging station availability and resale value directly influence adoption intention. Interestingly, attitude, subjective norms, and perceived behavioral control were not significant predictors. The findings suggest that in emerging markets, practical and economic concerns outweigh psychological or emotional drivers. These insights offer guidance for policymakers and businesses to focus on infrastructure development and value-enhancing strategies to accelerate EV adoption.
Electronic Banking Service Quality and Product Features on and Satisfaction Customer Loyalty Bank BJB Bandung Area Setiarini, Mersiana; Jusuf, Eddy; Juju, Undang
Jurnal Ilmiah Sumber Daya Manusia Vol 7 No 1 (2023): September: JENIUS (Jurnal Ilmiah Sumber Daya Manusia)
Publisher : Universitas Pamulang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32493/JJSDM.v7i1.33942

Abstract

This research is motivated by the demands of the duties and obligations of the banking world, which not only make customers interested, but also make these customers a source of profit for the bank but also make customers loyal. The results of this research provide an overview of Bank BJB in its services to its customers. The population of this research is bank bjb customers with a research sample of 400 respondents. In order to achieve the objectives of this research, questionnaire data from respondents were tested using the Structural Equation Modeling (SEM) method using LISREL 8.8 software. Based on the research results, it was found that service quality and bjb Electronic Banking product features significantly influence consumer satisfaction, service quality influences customer satisfaction, product features influence customer satisfaction, customer satisfaction influences customer loyalty.