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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Journal of Agricultural Science and Biotechnology Bumi Lestari Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Nandur
Khamdan Khalimi
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science

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Kajian Potensi Bakteri Lumpur Lapindo sebagai Agens Hayati terhadap Pyricularia oryzae dan Agens Biostimulan pada Tanaman Padi ROYAN PRACAHYO; KHAMDAN KHALIMI; GEDE WIJANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT The Study of Potential of Lapindo Mud Bacteria as Natural Agent Pyricularia oryzae and Biostimulant agent on Rice Rice (Oryza sativa L.) is the staple food of more than 60% of the world’s population. One of the cause for the low productivity of rice in indonesia is the occurrences of rice blast disease caused by Pycularia oryzae . This study was done to evaluate the potential use of  bacteria isolated from Lapindo mud as bio-control  agents  against  P. oryzae and bio-stimulant agents in rice plant. The result showed that three isolate of  bacteria isolated from Lapindo mud namely AA1, 20M14, and ZB11 significantly (P<0,05) inhibited the growth of  P. oryzae on Potato Dextrose Agar (PDA) medium with  inhibitory  activity  varied  85,07%, 86,58%, and 70,82%. Ten isolates of  bacterial  isolated from Lapindo mud significantly improved the growth of rice seedling. In which the plant heigth, number of root, content of chlorophyll in the leaf, number of  lateral  root  length of root, and the fresh and dry weight of plant significantly (P<0,05) higher on treated plant when compared to control. This is result suggested that three isolates of bacteria isolate from Lapindo mud can be further developed as bio-control agents to inhibit the P. oryzae growth and ten isolates of bacteria isolated from Lapindo mud can be used as bio-stimulan agents.   Key words: lapindo mud bacteria, Pyricularia oryzae, rice
Uji Aktivitas Antijamur Bacillus siamensis C7B terhadap Jamur Colletotrichum scovillei Penyebab Penyakit Antraknosa pada Tanaman Cabai Besar (Capsicum annuum L.) PARDIANTA PATRISIUS SINAGA; KHAMDAN KHALIMI; DEWA NGURAH SUPRAPTA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.4, Oktober 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Test of Antifungal Activity of Bacillus siamensis C7B Against Fungus Colletotrichum scovillei Causing Anthracnose Disease on Chili Pepper (Capsicum annuum L.) Colletotrichum scovillei is one of the species of fungi that causes anthracnose disease on chili peppers. The Purpose of this study was to determine the potential of B. siamensis C7B in inhibiting the growth of the fungus C. scovillei that causes anthracnose disease in large chili (C. annuum L.). This study was conducted in vitro by testing the antifungal activity of Bacillus siamensis C7B against three isolats of C. scovillei namely TBCR, SGCR, and GRCR on potato dextrose agar (PDA) and potato dextrose broth (PDB) media. The test results indicate that inhibition of the bacteria B. siamensis C7B able to inhibit the growth of fungi isolates of C. scovillei namely TBCR, SGCR, and GRCR with the percentage of inhibition respectively by 94,91%, 92,66%, and 91,47% when compared to the control on the observation of 21 days after inoculation. The inhibition test results of B. siamensis C7B filtrate at a concentration of 50% showed that the B. siamensis C7B filtrate was able to inhibit the growth of C. scovillei TBCR isolates with a percentage of inhibition activity of 90,27% when compared to control. The results of inhibition test of B. siamensis C7B on the formation of C. scovillei biomass of isolate TBCR showed that B. siamensis C7B was able to inhibit the formation of fungal biomass by 73,81% when compared to control. Further study is needed to evaluate the effectiveness of B. siamensis C7B to control anthracnose disease in vivo on chili pepper.
Pemanfaatan Bakteri Azotobacter untuk Meningkatkan Efisiensi Penggunaan Pupuk Urea pada Tanaman Padi (Oryza sativa L.) NI MADE INDRA PUSPAWATI; KHAMDAN KHALIMI; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Utilization of Azotobacter Bacteria to Increase the Efficiency of Urea Fertilizer Use in Rice Plants (Oryza sativa L.) Rice is a food plant in the form of grasses (Gramineae) originating from the tropical and subtropical continents of Asia and West Africa. Nearly half of the world's population, including Indonesia, uses rice as the staple food consumed to meet food needs. Based on BPS data, the population shows an increase every five years. It is inversely proportional to the decreasing total rice production. One of the efforts to improve the cultivation technology package and increase the quality of intensification is the use of Azotobacter bacteria as a biofertilizer. Based on the isolation results, 52 Azotobacter isolates were found grown on Abhys mannitol agar. Based on the results of the selection of Azotobacter bacterial isolates, it was found that four Azotobacter bacterial isolates had the best effect on root growth of rice plants, namely the Azotobacter PD3, PD23, PD48, and PD51 bacterial isolates. The test results with GC-MS showed that 2 compounds were thought to contribute to increasing the growth of rice plants. These compounds were n-Hexadecanoic acid and oleic acid, which were included in the fatty acid group. The isolates of the Azotobacter PD3, PD48, PD51 bacteria were able to increase the efficiency of using urea by 25-50%. In comparison, the PD23 bacteria were able to increase the efficiency of using urea by 25% based on the number of tillers.
Uji Aktivitas Antijamur Ekstrak Biji Keben (Barringtonia asiatica (L.) Kurz) terhadap Curvularia verruculosa Penyebab Penyakit Bercak Curvularia pada Tanaman Padi (Oryza sativa L.) I GEDE KARTA SATRIA WIBAWA; DEWA NGURAH SUPRAPTA; KHAMDAN KHALIMI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Antifungal Activity of Keben (Barringtonia asiatica (L.) Kurz) Seed Extract against Curvularia verruculosa the Causal Agent of Curvularia Leaf Spot Disease on Rice (Oryza sativa L.)This research was conducted to determine the effectiveness of keben (Barringtonia asiatica (L.) Kurz) seed extract to control Curvularia verruculosa the causal agent of curvularia leaf spot disease on rice. The extract concentration of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% and 1.0% was tested on in vitro test to determine the minimum inhibitory concentration (MIC) and inhibition percentage. Crude seed extract was partitioned and analyzed using GC-MS to determine the active antifungal compound. Concentration of 0.6%, 0.7%, 0.8%, 0.9% and 1.0% is tested on in vivo test in a greenhouse to determine the effectiveness of keben seed extract formula to control the curvularia leaf spot disease on rice. The result shows that the MIC of keben seed extract was 0.5% with inhibitory percentage that increases from 0.1% to 0.5% and cause 100% inhibitory on 0.6% concentration and above. GC-MS analysis showed antifungal compound such as Butyl Hydroxy Toluene; Dodecanoic acid, methyl ester; Methyl tetradecanoate; Benzoic acid 2, 5-bis (trimethylsiloxy)-trimethylsilyl ester; 1-Tetradecanol; Hexadecanoic acid, methyl ester; Octasiloxane, 1, 1, 3, 3, 5, 5, 7, 7, 9, 9, 11, 11, 13, 13, 15, 15-hexadecamethyl-; 13-Docosenoic acid, methyl ester (Z)- and 1, 2-Benzenedicarboxylic acid, dioctyl ester are present in keben seed. Greenhouse test showed that keben seed extract can suppress curvularia leaf spot disease from 25.16% to 2.57%.
Pengembangan Formula Biofungisida dan Aplikasinya dalam Mengendalikan Penyakit Layu Fusarium pada Tanaman Tomat (Solanum lycopersicum L.) DONI SITUMORANG; KHAMDAN KHALIMI; TRISNA AGUNG PHABIOLA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.4, Oktober 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Development of a Biofungicide Formula and Its Application in Controlling Fusarium Wilt in Tomato Plants (Solanum lycopersicum L.) Fusarium oxysporum fsp. lycopersici is one of the pathogenic fungi that causes Fusarium wilt disease in tomato plants and is very detrimental in tomato cultivation. Fusarium wilt disease control can be done by using a biofungicide formula that does not damage the environment. Biofungicide is the use of biological agents as active ingredients combined with natural carrier agents to form a biofungicide formula. The fungus Gliocladium sp. is a biological agent that can be used as an active ingredient in a biofungicide formula. This study aims to test the ability of Gliocladium sp. in inhibiting the growth of the fungus F. oxysporum fsp. licopersici in vitro and tested the ability of the formula with the active ingredient Gliocladium sp. in suppressing the incidence of wilt disease in vivo. The results of the inhibitory test of Gliocladium sp. against the growth of the fungus F.oxysporum fsp. lycopersici showed that the treatment of Gliocladium sp. can suppress the growth of fungal colonies F. oxysporum fsp. lycopersici with the percentage of inhibition of 99.21%. Testing of biofungicide formulas in vivo shows that the four biofungicide formulas namely F1, F2, F3 and F4 are able to control Fusarium wilt disease in tomato plants with a wilt disease percentage of 0%.
Isolasi dan Identifikasi Rizobakteri dari Rizosfer Kacang Tanah dan Uji Efektivitasnya dalam Mengendalikan Penyakit Layu Fusarium pada Tanaman Tomat SOLICHATUN SOLICHATUN; KHAMDAN KHALIMI; I MADE SUDARMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.4, Oktober 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Rhizobacteria from Peanut Rhizosphere to Control Fusarium Wilt on Tomato Tomato is one of the important horticultural in Indonesia. One obstacle in the cultivation of tomato is wilt disease caused by Fusarium oxysporum f.sp. lycopersici. Naturally soil microorganisms have the potential to suppress the development of pathogens in the soil. Population of microorganisms around rhizosphere is higher than not in rhizosphere both in quantity and quality. Legumes are known have positive effect to the microorganims in the soil due to rich N from root exudates.The purpose of this research is to determine the ability of rhizobacteria that were isolated from the rhizosphere of peanut in controlling F. oxysporum f.sp. lycopersici causes Fusarium wilt disease. Rhizobacteria as antagonist agent isolated from the rhizosphere of peanut from 3 places and tested against F. oxysporum f.sp. lycopersici in vitro and in vivo. Identification rhizobacteria with Oxoid Microbact GNB Kit.In this study, 61 isolates obtained from the rhizosphere of peanuts. However, only 4 isolates could inhibit Fusarium oxysporum f.sp. lycopersiciin vitro and only 2 isolates could identified certainly. One isolate identified as Klebsiella pneumoniae and one isolate as Stenotrophomonas maltophilia with percentage of probability above 90%. There needs to be more research on the stability of rhizobacteria in suppressing the occurance Fusarium wilt disease on tomato. Keywords: Tomato, Fusarium, and Rhizobacteria.
Uji Efektivitas Agen Hayati Dari Rizosfer dan Filosfer Tanaman Solanaceae untuk Mengendalikan Penyakit Antraknosa pada Tanaman Cabai Rawit (Capsicum frutescens L.) HENRI PAN BAGUS MULIA NAPITUPULU; KHAMDAN KHALIMI; DEWA NGURAH SUPRAPTA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.1, Januari 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Effectiveness of biological agents from Solanaceae plants rhizosphere andfilosphere to control Antrachnose disease in Chili Pepper Plants (Capsicum frutescens L.). The disease antrachnose caused by the fungus Colletotrichum truncatum canattack the chili fruit at any stage, but it will not attack the leaves or stems. The use ofbiological agents from the rhizosphere and filosphere is a viable alternative to controlthe anthracnose disease. Biological agents work selectively and better for theecosystem. The goal of this research is to identify the effectivenes of biological agentsfrom rhizospheres and filospheres solanaceae plant to control C. truncatum that causesantrachnose disease in Chili Pepper Plants (Capsicum frutescens L.). This researchtakes place in vitro and the field this research shows that TmFr4 and TrRr7 treatmentcan inhibit the growth of C. truncatum in vitro with a high percentage of inhibition90,58 % and 91,23 %. The TrRr7 bacterial isolate can inhibit the anthracnose diseasefrom 95,23% to 64,23 % and able to reduce the intensity from 66,59 % tob 39,06 %while TmFr4 fungal isolates can inhibit the desiase from 95,23 % to 68,81 % andreduce its intensity from 66,59 % to 47,03 % base on the field test. Further study isneeded to analyze the stability of TmFr4 fungal isolate and TrRr7 bacterial isolatebiological control of C. truncatum in the field.
Kajian Potensi Bakteri Lumpur Lapindo sebagaiAgens Hayati terhadap Pyriculariaoryzaedan Agens Biostimulan pada Tanaman Padi ROYAN PRACAHYO; KHAMDAN KHALIMI; GEDE WIJANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT The study of bacteria isolated from Lapindo mud as a bio-control  agents  against  P. oryzae and bio-stimulant agents in rice plant Rice (Oryza sativa L.) is the staple food of more than 60% of the world’s population. One of the cause for the low productivity of rice in indonesia is the occurrences of rice blast disease caused by Pycularia oryzae . This study was done to evaluate the potential use of  bacteria isolated from Lapindo mud as bio-control  agents  against  P. oryzae and bio-stimulant agents in rice plant. The result showed that three isolate of  bacteria isolated from Lapindo mud namely AA1, 20M14, and ZB11 significantly (P<0,05) inhibited the growth of  P. oryzae on Potato Dextrose Agar (PDA) medium with  inhibitory  activity  varied  85,07%, 86,58%, and 70,82%. Ten isolates of  bacterial  isolated from Lapindo mud significantly improved the growth of rice seedling. In which the plant heigth, number of root, content of chlorophyll in the leaf, number of  lateral  root  length of root, and the fresh and dry weight of plant significantly (P<0,05) higher on treated plant when compared to control. This is result suggested that three isolates of bacteria isolate from Lapindo mud can be further developed as bio-control agents to inhibit the P. oryzae growth and ten isolates of bacteria isolated from Lapindo mud can be used as bio-stimulan agents. Keywords:Bacteria, Pycularia oryzae, Oryza sativa L.
Uji Antagonistik Bacillus siamensis dan Paenibacillus polymyxa Terhadap Colletotrichum gloeosporioides KLCR2 Penyebab Penyakit Antraknosa pada Buah Cabai Rawit (Capsicum frutescens L.) NI KOMANG SRI BAWANTARI; DEWA NGURAH SUPRAPTA; KHAMDAN KHALIMI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.3, Juli 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Antagonistic test of Bacillus siamensis and Paenibacillus polymyxa against the growth of Colletotrichum gloeosporioides the cause of Antracnose disease in cayenne pepper (Capsicum frutescent L.) Colletorichum gloeosporioides is one of latent pathogens that can infect several agricultural crops. This pathogenic fungus is very detrimental to the production of cayenne pepper (Capsicum frutescens L.). The purpose of this research was to determine the ability of Bacillus siamensi and Paenibacillus polymyxa in inhibiting the growth of C. gloeosporioides KLCR2 causes of anaplosal disease. Research was conducted in Biopesticide laboratory. Results showed that treatment with antagonistic bacteria significantly (P<0.05) inhibited the growth of C. gloeosporioides on potato dextrose agar (PDA) medium. Treatment with B. siamensis was able to inhibit the growth of C. gloeosporioides with inhibitory activity by 96.18%. While for the treatment with P.polymyxa the inhibitory activity was 84.79%. Treatment with B. siamensi depressed the biomass of C. gloeosporioides KLCR2. In this study, treatment with cell-free filtrate of B. siamensis at concentration of 50% was able to suppress the development of C. gloeosporioides with a percentage of inhibitory activity by 94.15%. Further study is necessary to evaluate the ability of the B. siamensis to inhibit the development of C. gloeosporioides KLCR2 in vivo on cayenne pepper fruit.
Identifikasi Senyawa Antijamur dari Agens Hayati Rizoplan KEZIA KEZIA; KHAMDAN KHALIMI; TRISNA AGUNG PHABIOLA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.4, Oktober 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Identification of Antifungal Compounds From Biological Agents of Rhizoplan Fusarium wilt disease that attacks plants is caused by Fusarium oxysporum, this disease can cause significant losses to plants. The use of rhizoplan bacteria is considered as an alternative to control fungal growth. The use of selective and environmentally friendly rhizoplan bacterial control. The purpose of this study was to identify antifungal compounds in bacterial rhizoplans. The results showed that rhizoplan bacteria were able to inhibit the growth of F. oxysporum f.sp. lycopersici fungal colonies on potato dextrose agar (PDA) media with an inhibition percentage of 88.24% when compared to the control. The results of the filtrate test of rhizoplan bacteria at a concentration of 50% were able to inhibit the growth of F. oxysporum f.sp. lycopersici with an inhibition percentage of 90.14%. The antifungal compounds produced by rhizoplan bacteria isolate RbJN10 on Gas Chromatography Mass Spectrometry (GC-MS) analysis were Dihydro-3- (2H)-thiophenone; 2(5H)- Furanon; 2(3H)- Furanone,5-methyl; 2- Furancarboxaldehyde, 5-methyl; 2,5Dimethylfuran-3,4(2H, 5H)-dione; 2- Furancarboxylic Acid; Methyl 2-furoate; 1,2-Ethanadiol,1-(2-furanyl)-; 5- hydroxymethylfurfural.
Co-Authors ANINDA OKTAVIA RAHARINI Dewa Ngurah Suprapta DONI SITUMORANG GEDE WIJANA GUSTI AYU KOMANG CANDRA PARWATI Gusti Ngurah Alit Susanta Wirya HENRI PAN BAGUS MULIA NAPITUPULU I Dewa Nyoman Nyana I G. R. M. TEMAJA I Gede Karta Satria Wibawa I GEDE KARTA SATRIA WIBAWA I Gusti Made Wira Kesuma Jaya I GUSTI NGURAH RAKA I Ketut Siadi I MADE SUDARMA I MADE SUDARMA I NYOMAN GEDE ASTAWA I PUTU WIRYA SUPUTRA IDA AYU ISTRI MAYADIANTI KEZIA KEZIA KOMANG ADI MAHARTHA KRISNA SANUBARI PURBA Ni Kadek Desy Andya Dewi NI KOMANG SRI BAWANTARI Ni Luh Gede Sriyanti Ni Luh Putu Citra Innosensia NI MADE INDRA PUSPAWATI Ni Made Intan Maulina NI WAYAN SUNITI NURI MANDAN SARI PARDIANTA PATRISIUS SINAGA Putu Perdana Kusuma Wiguna Retno Kawuri ROYAN PRACAHYO SAMI MAIDA SIJABAT SOLICHATUN SOLICHATUN SUSAN CAMILA FOEH TRISNA AGUNG PHABIOLA Valente Quintao WAYAN ADIARTAYASA