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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Journal of Agricultural Science and Biotechnology Bumi Lestari Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Nandur
Khamdan Khalimi
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science

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Penggunaan Streptomyces sp. Sebagai Biokontrol Penyakit Layu Pada Tanaman Cabai Merah (Capsicum annuum L.) yang Disebabkan Oleh Fusarium oxysporum f.sp. capsici ANINDA OKTAVIA RAHARINI; RETNO KAWURI; KHAMDAN KHALIMI
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.962 KB)

Abstract

A research has been conducted to find out Streptomyces bacteria at Bukit Jimbaran, to inhibitionpotency of Streptomyces sp. to pathogenic fungi Fusarium oxysporum f.sp. capsici, and to find outantifungal activity of Streptomyces filtrate to F.oxysporum f.sp. capsici in chili (Capsicum annuumL.) plants. Streptomyces sp. isolation was done by platting method with selective media YMA (ISP4).Identification of Streptomyces sp. used Bergey’s book entitled Manual Determinative Bacteriology.Test inhibition against F.oxysporum f.sp. capsici and in vivo test used by dying the roots of the chili(C.annuum L.) plant with F.oxysporum f.sp. capsici and after 30 seconds the roots were dying withStreptomyces sp. culture, furthermore sterile soil on polybag watered by F.oxysporum f.sp. capsicispore and Streptomyces sp. culture at the same time. The result found five isolates Streptomyces sp.with different morphological. The antagonis test showed Streptomyces sp. 4 had ability (82%) againstFusarium, Streptomyces sp.1 (72%), Streptomyces sp.2 (64%), Streptomyces sp.3 (76%), andStreptomyces sp. 5 (32%). All Streptomyces suppressed the growth of Fusarium on chili plants inglass house (p<0,05). Streptomyces sp.4 suppressed Fusarium wilt disease in chili from 80% in controlto 8%.
EFFECT OF BACTERIAL VOLATILE COMPOUNDS ON PAKCOY (Brassica rapa L.) GROWTH PROMOTION Trisna Agung Phabiola; Khamdan Khalimi; Putu Perdana Kusuma Wiguna
International Journal of Biosciences and Biotechnology Vol 9 No 2 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v09.i02.p05

Abstract

This research was aimed to test of the ability of MVOC-producing bacteria to increase plant growth of Pakcoy (Brassica rapa L.). The methodology including testing the ability of MVOC-producing rhizobacteria in Pakcoy plant growth enhancement, MVOC extraction and analysis of compounds in MVOC Extracts using Gas Chromatography. Different bacterial species produce different MVOC. S. maltophilia Sg3 emitted 20 MVOC compounds and MVOC that contribute to increasing plant growth, namely oxalic acid, cyclohexyl undecyl ester, 2-Furancarboxaldehyde, 5-methyl-, 1,2 butanediol, and Piperazine. E. asburiae MjSg48 emitted 12 MVOC compounds and those that contributed to increasing plant growth were oxalic acid, cyclohexyl dodecyl ester and 4-methyl oxazole. E. asburiae TK24 emitted 27 MVOC compounds and those that contributed to increasing plant growth were oxalic acid, isohexyl neopentyl ester, thiazole, Oxalic acid, and cyclohexyl decyl ester. Meanwhile P. rettgeri Al2TT emitted 13 MVOC compounds and those that contributed to increasing plant growth were oxalic acid, diisohexyl ester, and Pyridine, 2,3,4,5-tetrahydro.
Eksplorasi Agens Hayati Rizoplan Sebagai Pengendali Jamur Fusarium oxysporum f.sp. cubense I Gusti Made Wira Kesuma Jaya; Khamdan Khalimi; Ni Wayan Suniti
Nandur Vol 2 No 1 (2022)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Fusarium wild or wilt disease that attacks banana plants is caused by the pathogenic fungus Fusarium oxysporum f.sp. cubense This disease is difficult to control because it lasts a long time in the soil by forming chlamydospores. Currently, the control of this disease is still using synthetic fungicides, but this method can cause damage to the ecosystem. Biopesticide is one of the environmentally friendly control of plant pathogens because biopesticides use biological agents. The purpose of this study was to export rhizoplan bacteria from banana plant roots which have antifungal activity against F. oxysporum f.sp. cubense. The results showed that rhizoplan bacteria were able to inhibit the growth of Fusarium oxysporum f.sp. cubense fungal colonies on potato dextrose agar (PDA) media with an inhibitory percentage of 90.90% when compared to the control. The results of the bacterial filtrate test of rhizoplan at a concentration of 50% were able to inhibit the growth of the fungus Fusarium oxysporum f.sp. cubense with an inhibitory percentage of 92.72%.
THE EFFECTIVENESS OF RHIZOBACTERIA AS BIOPROTECTANTS TO MITIGATE FUSARIUM WILT DISEASE AND AS BIOSTIMULANTS TO IMPROVE THE GROWTH OF CHILI (Capsicum annuum) Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya; I Putu Wirya Suputra; Ni Luh Putu Citra Innosensia
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p04

Abstract

The aims of this study were to examine the potential of Rhizobacteria as bioprotectants and biostimulants on chili. The potential as bioprotectants was tested by the ability of these Rhizobacteria to inhibit the growth of the pathogenic fungus Fusarium oxysporum f.sp. capsici in vitro. Moreover, as biostimulants was tested by the ability of these rhizobacteria to produce IAA compounds and their ability to increase the plant growth parameter of chili. Four rhizobacteria were challenged for antagonistic activity against F. oxysporum f.sp. capsici. Chili seeds in this experiment were soaked in four different Rhizobacteria suspension for 15 minutes. For control, seeds were soaked with sterile water instead of Rhizobacteria suspension. Results of this experiment showed that four isolates of Rhizobacteria showed strong inhibitory activity against F. oxysporum f.sp. capsici on PDA medium. Percentage of inhibitory activity varied from 77,33% to 89,79%. The application of four isolates recorded significantly increased the plant growth parameters of chili. The plant height, number of leaves, leaf area, root length, shoot dry mass, root dry mass, and chlorophyll content on treated plants significantly higher than those of untreated control plants according to the Duncan’s multiple range test (P<0.05%). These results indicate that the tested Rhizobacteria can be used as bioprotectants because they can inhibit the growth of F. oxysporum f.sp. capsici and simultaneously as a biostimulant for the ability to produce IAA compounds and stimulate the growth of chili.
Penemuan Kandungan Asam Giberellin (GA3) pada Ekstrak Beberapa Jenis Tumbuhan I NYOMAN GEDE ASTAWA; KHAMDAN KHALIMI
Agrotrop : Journal on Agriculture Science Vol 13 No 1 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i01.p13

Abstract

The Discovery of the Content of Giberellin Acid (GA3) in Extracts of Several Plant Spesies. Several studies have found that the application of growth regulator Giberellin can induce seedless in seeded varieties and also improve fruit quality in non-seeded varieties of grapes. It is also directly responsible for cell division and is used in horticulture to promote seed germination. The high price makes it unaffordable for farmers, especially in Indonesia, so it is necessary to look for other sources of GA3 which comes from extracts of plant species in Indonesia. The aims of this study were: To detect the content of gibberellin acid (GA3) in several plant species; The research was conducted at the Agronomy & Horticulture Laboratory, Faculty of Agriculture, Udayana University. The results of the study found that fresh corn kernels (Zea mays L.), young bamboo shoots (Bambusa sp.), fern leaves (Diplazium esculentum), spinach leaves (Amaranthus spp) and shallot bulbs (Allium cepa), contained as much as 215 µg GA3 mL-1, 257 µg GA3 mL-1, 260 µg GA3 mL-1, 154 µg GA3 mL-1 and 227 µg GA3 mL-1 respectively. It can be concluded that extracts of plant materials contain GA3 and can be used as a substitute for synthetic GA3.
Co-Authors ANINDA OKTAVIA RAHARINI Dewa Ngurah Suprapta DONI SITUMORANG GEDE WIJANA GUSTI AYU KOMANG CANDRA PARWATI Gusti Ngurah Alit Susanta Wirya HENRI PAN BAGUS MULIA NAPITUPULU I Dewa Nyoman Nyana I G. R. M. TEMAJA I GEDE KARTA SATRIA WIBAWA I Gede Karta Satria Wibawa I Gusti Made Wira Kesuma Jaya I GUSTI NGURAH RAKA I Ketut Siadi I MADE SUDARMA I MADE SUDARMA I NYOMAN GEDE ASTAWA I PUTU WIRYA SUPUTRA IDA AYU ISTRI MAYADIANTI KEZIA KEZIA KOMANG ADI MAHARTHA KRISNA SANUBARI PURBA Ni Kadek Desy Andya Dewi NI KOMANG SRI BAWANTARI Ni Luh Gede Sriyanti Ni Luh Putu Citra Innosensia NI MADE INDRA PUSPAWATI Ni Made Intan Maulina NI WAYAN SUNITI NURI MANDAN SARI PARDIANTA PATRISIUS SINAGA Putu Perdana Kusuma Wiguna Retno Kawuri ROYAN PRACAHYO SAMI MAIDA SIJABAT SOLICHATUN SOLICHATUN SUSAN CAMILA FOEH TRISNA AGUNG PHABIOLA Valente Quintao WAYAN ADIARTAYASA