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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Journal of Agricultural Science and Biotechnology Bumi Lestari Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Nandur
Khamdan Khalimi
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science

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Potensi Bakteri Endofit Dalam Menekan Pertumbuhan Phytophthora Palmivora (Butler) Secara In Vitro SUSAN CAMILA FOEH; I GEDE RAI MAYA TEMAJA; KHAMDAN KHALIMI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Potential of Endophytic Bacteria in Suppressing The Growth of Phytophthorapalmivora (Butler) in Vitro The purpose of this study was to determine the potential of endophytic bacteria in controlling cocoa fruit rot caused by the fungus Phytophthora palmivora. The studywas conducted in vitro and direct test on cocoa fruit. The results of the inhibitory testshowed that 4 isolates of endophytic bacteria had the best inhibition for suppressingthe growth of Phytophthora palmivora. The in vitro inhibitory test showed, thetreatment of TN41 isolates was able to inhibit Phytophthora palmivora with thehighest inhibition percentage of 92.52% when compared to the control at theobservation of 5 days after inoculation. The direct test on cocoa fruit showed thetreatment of TN41 isolate had the least area of spot that is 0.20 mm2 at 7 days after inoculation. Further research needs to be conducted related to the stability ofendophytic bacteria isolate TN41 as an antagonist agent against Phytophthorapalmivora.
Uji Efektivitas Rizobakteri sebagai Agen Antagonis terhadap Fusarium oxysporum f.sp. capsici Penyebab Penyakit Layu Fusarium pada Tanaman Cabai Rawit (Capsicum frutescens L.) KOMANG ADI MAHARTHA; KHAMDAN KHALIMI; ALIT SUSANTA WIRYA GUSTI NGURAH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.3, Juli 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Effectiveness Test of Rhizobacteria as Antagonist Agents against Fusarium oxysporum f.sp. capsici Causes Fusarium Wilt Disease in Chili Pepper  Plants(Capsicum frutescens L.) Utilization of rhizobacteria as antagonist agents Fusarium oxysporum f.sp. capsici was recommended. The objectives of this research were conducted to evaluate the effectiveness of rhizobacteria as an agents of biocontrol. F. oxysporum f.sp. capsici was put in the center of PDA medium (control). Rhizobacteria as antagonistic fungus was inoculated four side onto PDA medium and then F. oxysporum f.sp. capsici was put in the center of PDA medium. Result of this research, Klebsiella pneumoniae isolates KTNA2 showed strong inhibitory activity against F. oxysporum f.sp. capsici. Percentage of inhibitory activity at 89,65%. Aplication of Pantoea agglomerans isolates GTA24, Stenotrophomonas maltophilia isolates KTTA4, Klebsiella pneumoniae isolates GSA6, and Klebsiella pneumoniae isolates KTNA2 in the open field could suppress the disease incidence about 33,33%, 43,33%, 56,67%, and 63,33% respectively. The disease incidence on control about 80%. Keywords: rhizobacteria, antagonist agents, and Fusarium oxysporum f.sp. capsici
Uji Efikasi Formulasi Rizobakteri Pantoea agglomerans GTA24 dalam Mengendalikan Penyakit Rebah Semai yang Disebabkan oleh Sclerotium rolfsii pada Tanaman Kedelai GUSTI AYU KOMANG CANDRA PARWATI; KHAMDAN KHALIMI; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Efficacy Test of Pantoea agglomerans GTA24 rhizobacteria Formulations in Controling Damping off  Disease Caused By Sclerotium rolfsii on Soybean Plants The experiment aimed to determine the effectiveness of the P. agglomerans GTA24 rhizobacteria formulations in controlling damping off diseases. The experiment was conducted in vitro and in greenhouse test. The experiment was a randomized complete blocks with five replications. The treatment consists of four types of P. agglomerans GTA24 rhizobacteria formulations is pellets formula, powder formula, compost formula, and the gel formula. The results showed that the application of P. agglomerans GTA24 formulations significantly inhibited the fungal growth. P. agglomerans GTA24 showed strong inhibitory activity agains    Sclerotium rolfsii on PDYA medium percentage of inhibitory activity about 96,30%. The lowest disease incidence was attained by the treatment of gel formula, in which only 15,56% of the soybean plants were infected.   Keywords : rhizobacteria formulations, damping off disease, soybean plants
Pengaruh Aplikasi Formula Pantoea agglomerans Terhadap Aktivitas Antioksidan dan Kandungan Klorofil Daun Tanaman Strowberi TRISNA AGUNG PHABIOLA; KHAMDAN KHALIMI
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

The objectives of this experiment were conducted to evaluate the effectiveness of Pantoeaagglomerans formulations to increase antioxidant activities and chlorophyll content of strawberry plants.P. agglomerans was formulated in the forms of gel and powder. Measurement of peroxidase activitycarried out 7 days after application of P. agglomerans formulations on strawberry plants. The mannerin which the Cohen prosedure suggested by Simon and Rose (1970) and has been modified. Totalchlorophyll content (SPAD unit) was determined with a chlorophyll-meter SPAD-502. Some growthparameters were observed such as plant height, number of leaves, and leaf area. Results of this studyshowed that application of P. agglomerans formulations could increase the peroxidase activity. Peroxidaseactivity on treated plants increased by 47.17% to 50.16% in comparison with un-treated plants. Totalchlorophyll content on treated plants increased by 23.81% to 28.22% in comparison with un-treatedplants. These results suggested that application of P. agglomerans formulations could increase the totalchlorophyll content and antioxidant activity of strawberry plants.
Streptomyces sp. Sebagai Biofungisida Patogen Fusarium oxysporum (Schlecht.) f.sp. lycopersici (Sacc.) Snyd. et Hans. Penyebab Penyakit Layu Pada Tanaman Tomat (Solanum lycopersicum L.) NURI MANDAN SARI; RETNO KAWURI; KHAMDAN KHALIMI
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

A research was conducted to isolate Streptomyces sp. of soil Udayana University campus in theBukit-Jimbaran, to obtain the most effective Streptomyces sp. which is effective in inhibit the growth ofFusarium oxysporum f.sp. lycopersici, and to test response of tomato plants with Streptomyces sp.culture against Fusarium wilt desease. Implementation phases of the research consisted of isolation andidentification of Streptomyces sp, test the inhibition against F. oxysporum f.sp. lycopersici, and in vivotest used by dyeing the roots of the tomato plant (Solanum lycopersicum) with Fusarium spores andafter 30 seconds the roots were dyeing Streptomyces culture. Furthermore, sterile soil in polybagwatered by Fusarium spores and Streptomyces culture at the same time. Based on morphologicalcharacteristic it found five isolates of Streptomyces sp.. The antagonist test showed Streptomyces sp.1 had ability (75%) against Fusarium, Streptomyces sp 2 (68,3%), Streptomyces sp. 3 (71,6%),Streptomyces sp. 4 (63,3%), and Streptomyces sp. 5 (21,6%). All Streptomyces suppressed thegrowth of Fusarium on tomato plants in glass house (p<0,05). Streptomyces sp.3 suppressed Fusariumwilt disease in tomato from 88% in control to 20%.
Aplikasi Rizobakteri Pantoea agglomerans untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Jagung (Zea mays, L.) Varietas Hibrida BISI-2 I GUSTI NGURAH RAKA; KHAMDAN KHALIMI; I DEWA NYOMAN NYANA; I KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 2 No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

This experiment was conducted in the village of Sibang Gede, District of Abiansemal, BadungRegency on November 2011 until February 2012. The research objective is to enhance the growth andyield of maize hybrid BISI-2 using rhizobacteria Pantoea agglomerans as PGPR in compostformulations. This study used a randomized block design (RBD) single factor with 5 treatments and 5replications. The treatments tested were P. agglomerans BS7a, P. agglomerans BS7b, P. agglomeransBS2a, and P. agglomerans BS5a. All treatments P. agglomerans formulated in the form of compostand given a dose of 50 g/hole. As a control dose of compost 50 g/hole without PGPR. The resultsshowed that the application of rhizobacteria P. agglomerans increased the maximum plant height, netassimilation rate, crop growth rate, chlorophyll content of leaves, fresh weight of roots per plant, ovendried weight of stover per plant, number of seeds per plant, cob length, cob diameter, seed weightmoisture content of 12% per plant and seed yield of 12% water content per hectare.
Efektivitas PGPR Formulasi Kompos Dalam Meningkatkan Ketahanan Tanaman Kedelai terhadap Soybean Stunt Virus I KETUT SIADI; KHAMDAN KHALIMI; I DEWA NYOMAN NYANA; I GUSTI NGURAH RAKA
Agrotrop : Journal on Agriculture Science Vol 7 No 2 (2017)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Effectiveness of PGPR Compost Formulation in Improving Soybean Plant Resistance to Soybean Stunt Virus. Soybean stunt virus (SSV) is one of important obstacles of soybean production in Indonesia. This virus causes the stunting on soybean plant and may cause the yield losses up to 71%. Eight isolates of plant growth promoting rhizobacteria (PGPR) were isolated and tested for their efficacy to control SSV on soybean. Those isolates are Paj, Pak2, Pa1, Pa3, Pa4, BT, and KT. Application of PGPR was done by soaking the seeds in PGPR solution prior to planting and application of PGPR in compost formulation. Virus concentration and disease incidence were determined using DAS-ELISA. Results of this study showed that application of PGPR in compost formulation suppressed disease incidence caused SSV. Disease incidence on treated plants ranged between 10% to 25%, while all of plant (100%) on un-treated plants were infected. Peroxidase activity on treated plants increased by 80.25% to 97.33% in comparison with un-treated plants. These results suggested that application of PGPR in compost formulation could increase the resistance of soybean against SSV. Hence, PGPR can be considered as one of measures to control SSV on soybean.
Karakterisasi Biokimia Aeromonas hydrophila dan Potensinya Sebagai Pemacu Pertumbuhan Bibit Tanaman Melon Emas I KETUT SIADI; KHAMDAN KHALIMI; DEWA NYOMAN NYANA
Agrotrop : Journal on Agriculture Science Vol 4 No 1 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Biochemical Characterization of Aeromonas hydrophila and Its Potential for Growth Stimulatorof Golden Melo Seedlings.The results showed that this research got 20 nitrogen fix bacteria isolate,However, Pg2 isolate could increase the growth of golden melo. The treatment with Pg2 isolatesignificantly increased the golden melo growth. All the plant growth parameters of treated plants weresignificantly higher than those of un-treated control plants (P<0.05). The leaf chlorophyll content, plantgrowth rate, and fresh and dry weights of plant were significantly higher than those of un-treatedcontrol plants. Isolates identified as Enterobacter cloacae PG2 percentage of 98.05% probability.
KARAKTERISASICYMBIDIUM MOSAIC VIRUS (CYMMV) PADA TANAMAN ANGGREK KHAMDAN KHALIMI; KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 1 No 1 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Characterization ofCymbidium mosaic virus (CymMV) on Orchid Plant Orchids are affected by more virus disease problems than most crops, reducing their commercial values considerably. Orchid viruses are widespread in cultivated orchids, withCymbidium mosaic potexvirus (CymMV) being the most prevalent. CymMV high incidence in cultivated orchids has been attributed to the stability and ease of transmission of this virus through cultural practices. CymMV induces floral and foliar necrosis. The virus also reduce plant vigor and lower flower quality, which affect their economic value. The objective of the research is to characterize the virus causing mosaic or chlorotic and necrotic on orchids in West Java. A reverse transcription-polymerase chain reaction (RT- PCR) assays using oligonucleotide primers specific to CymMV were also successfully amplified the regions of the coat protein (CP) gene of the virus. Analysis by using sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) revealed that the virus have a major structural protein with an estimated molecular weight of 28 kDa. Aligments of partial nucleotide sequences of the CP gene displayed 86 to 92% homology to CymMV isolates from other countries.
POTENSI PSEUDOMONAS AERUGINOSA SEBAGAI AGENS BIOKONTROL TERHADAP SCLEROTIUM ROLFSII SACC. DAN BIOSTIMULAN PADA TANAMAN KEDELAI KHAMDAN KHALIMI; I GUSTI NGURAH RAKA; I KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 1 No 2 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Potential of Pseudomonas aeruginosa as Biological Control Agents against Sclerotium rolfsii Sacc. and Biological Stimulants on Soybean Plant. The objectives of this experiment were conducted to evaluate the effectiveness ofP. aeruginosa as an agent of biological control and biological stimulants. P. aeruginosawere tested for antagonistic activity against Sclerotium rolfsii Sacc. The test for antagonistic activity by bacteria was done through side by side culture. Soybean seeds that will be used in the rhizobacteria treatment were soaked in rhizobacteria suspension for 15 minutes. For wihout the treatment, seeds soaked with sterile water instead of rhizobacteria suspension. Results of this experiment,P. aeruginosa showed strong inhibitory activity againstS. rolfsii Sacc. on PDA medium. Percentage of inhibitory activity was 94,4%. Application of P. aeruginosa significantly increased the plant growth. The maximum plant hight, the maximum number of leaves, fresh and dry weights of root, fresh and dry weights of shoot, and chlorophyll content on treated plants significantly higher than those of un-treated control plants according to the Duncan’s multiple range test (P<0.05%). These results suggested that application of rhizobacteria could promote the plant growth and increase the yield.
Co-Authors ANINDA OKTAVIA RAHARINI Dewa Ngurah Suprapta DONI SITUMORANG GEDE WIJANA GUSTI AYU KOMANG CANDRA PARWATI Gusti Ngurah Alit Susanta Wirya HENRI PAN BAGUS MULIA NAPITUPULU I Dewa Nyoman Nyana I G. R. M. TEMAJA I GEDE KARTA SATRIA WIBAWA I Gede Karta Satria Wibawa I Gusti Made Wira Kesuma Jaya I GUSTI NGURAH RAKA I Ketut Siadi I MADE SUDARMA I MADE SUDARMA I NYOMAN GEDE ASTAWA I PUTU WIRYA SUPUTRA IDA AYU ISTRI MAYADIANTI KEZIA KEZIA KOMANG ADI MAHARTHA KRISNA SANUBARI PURBA Ni Kadek Desy Andya Dewi NI KOMANG SRI BAWANTARI Ni Luh Gede Sriyanti Ni Luh Putu Citra Innosensia NI MADE INDRA PUSPAWATI Ni Made Intan Maulina NI WAYAN SUNITI NURI MANDAN SARI PARDIANTA PATRISIUS SINAGA Putu Perdana Kusuma Wiguna Retno Kawuri ROYAN PRACAHYO SAMI MAIDA SIJABAT SOLICHATUN SOLICHATUN SUSAN CAMILA FOEH TRISNA AGUNG PHABIOLA Valente Quintao WAYAN ADIARTAYASA