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All Journal Reaktor Prosiding Semnastek Indonesian Journal of Chemistry JMM (Jurnal Masyarakat Mandiri) SAINTEK: Jurnal ilmiah Sains dan Teknologi Industri Jurnal Abdimas Madani dan Lestari (JAMALI) Widya Teknik

Wenny Irawaty

Chemical Engineering Department, Widya Mandala Catholic University Surabaya

Author-ID : 2955478

Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Energy Industrial & Manufacturing Engineering Materials Science & Nanotechnology Other

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FRACTIONATION OF PHENOLIC COMPOUNDS FROM KAFFIR LIME (Citrus hystrix) PEEL EXTRACT AND EVALUATION OF ANTIOXIDANT ACTIVITY Yusak Adi Wijaya; Daniel Widyadinata; Wenny Irawaty; Aning Ayucitra
Reaktor Volume 17 No. 3 September 2017
Publisher : Dept. of Chemical Engineering, Diponegoro University

Side effects of drug-based treatment observed in patients during degenerative diseases treatments has directed towards to the identification of plants with antioxidant activity. Kaffir lime peel was selected in this study. This work was aimed to investigate the effect of solvent (hexane, ethyl acetate and n-butanol) polarity on the fractionation of ethanolic crude extract and assess its antioxidative property by DPPH radical scavenging assay. The results show semi polar solvent of ethyl acetate exhibited the best solvent to extract phenolic compounds from ethanolic kaffir lime peel with total phenolic content detected was 0.12 mg Gallic Acid Equivalent/mg. The employment of solvents possessing different polarity resulted several fractions, i.e. hexane, ethyl acetate, n-butanol and residue with different type of phenolic compounds in each fraction. Accordingly, each fraction exhibited different antioxidant activity against free radical compound of DPPH. The N-butanol fraction demonstrated the strongest activity which is shown by lowest IC50 value among the fractions tested. The fraction of n-butanol exhibited the IC50 value of 0.44 mg/mL which means that only 0.44 mg of the fraction/mL is required to inhibit the neutralization of DPPH by 50%. Phenolic identification by High Performance Liquid Chromatography indicates the different phenolic compounds in each fraction that contribute to antioxidative property to different extent. Further investigation to identify these phenolic compounds will lead to further development of kaffir lime as natural antioxidant to treat specific degenerative diseases.

Antimicrobial Activity of Kaffir Lime Peel Extract against Streptococcus mutans Rosalie Purwanto; Jeni Pabontong; Ery Susiany Retnoningtyas; Wenny Irawaty
Reaktor Volume 18 No. 4 December 2018
Publisher : Dept. of Chemical Engineering, Diponegoro University

Kaffir lime peels contain polyphenols as natural antioxidant and antimicrobial agent. The aims of this study were to (1) extract phenolics compounds from kaffir lime peels using water, ethanol 70% and ethanol 96% as the solvent, and (2) assess the antibacterial activity of the extract against Streptococcus mutans which is the main cause of dental caries. Research methodology includes preparation and extraction of polyphenols from kaffir lime peels, preparation of mouthwash based-kaffir lime peels extracts and evaluation the mouthwash ability to inhibit the growth of Streptococcus mutans. The results show water exhibited the best solvent to extract polyphenols among the three solvents. The total phenolics content in the water extract was observed at 11.42±0.48 mg GAE/g, whilst in the two ethanolic extracts were 10.91±0.87 and 8.87±0.53 mg GAE/g for ethanol 70 and 96%, respectively. Consequently, the water-based extract performed the highest antimicrobial activity. The highest inhibition zone was demonstrated by 100% extract of concentration extract variation. Although the inhibition zone of the mouthwash was smaller than the commercial product, the extract has the potential to be developed as a safe mouthwash for long-term usage.Keywords: dental caries; kaffir lime; mouthwash; peel; phenolic; Streptococcus mutans

Heterogeneous Fenton Degradation of Batik Wastewater using Natural Pyrite Irawaty, Wenny; Yuliana, Maria; Wijaya, Christian J.; Retnoningtyas, Ery S.; Lourentius, Suratno; Puspitasari, Nathania; Hamidah, Umi
Reaktor Volume 23 No.3 December 2023
Publisher : Department of Chemical Engineering, Faculty of Engineering, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/reaktor.23.3.101-107

Heterogeneous Fenton processes with solid natural pyrite catalyst have gained attention for wastewater treatment. The improvement of degradation efficiency of natural pyrite is specific and therefore, it is still a challenging issue. This study was aimed to investigate the performance of Indonesian natural pyrite, without any purification, to treat the real wastewater obtained from batik home industry. To address this issue, several experiment variables such as pre-reaction between pyrite and hydrogen peroxide, hydrogen peroxide concentration, and pH were investigated. COD removal has been found to increase in a slightly acid condition (4.5 to 5.65) and inhibited at pHs of 8 and above (10). The results showed the presence of hydrogen peroxide was indeed greatly influence the wastewater degradation and the solution pH did not significantly change the wastewater degradation in the range of 3 and less than 8. Moreover, the pre-reaction period was found as the important part to initiate the degradation process. The optimum degradation efficiency was obtained by providing the pre-reaction time of 20 min with the addition of 326 mmol/L of H2O2 in the system and the mixture was reacted for 30 min at room temperature and real wastewater pH. Based on results observed in this study, it can be concluded that the batik wastewater can be degraded by natural pyrite up to 84% under reaction conditions studied. This finding offers great opportunity to further investigate and develop the heterogeneous Fenton process for real applications in industry.

Co-Authors Adriana Anteng Anggorowati Afendi, Yulius Andrew Joewono, Andrew Aning Ayucitra Chatarina Dian Indrawati Cicilia Setyabudi Daniel Widyadinata Ernest, Edward H Ery Susiany Retnoningtyas Felycia Edi Soetaredjo Gunarto, Chintya Hadi Hamidah, Umi Harianto, Octaviani Hartono, Sandy Budi Imelda Panjaitan Jeni Pabontong Kang Tuan Hok Lie, Jenni Lourentius, Suratno Puspitasari, Nathania Putro, Jindrayani Nyoo Retnoningtyas, Ery S. Rosalie Purwanto Santoso, Shella Permatasari Setiyo Gunawan Stefani Tanda Suryadi Ismadji Waloyo, L. Anang Setiyo Wardhani, Rinda Widharka, Maria Rosario Wijaya, Christian J. Wijaya, Christian Julius Wiwit Setyo Yuliana, Maria Yusak Adi Wijaya

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