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Daya Hambat Kombinasi Ekstrak Pegagan (Centella asiatica, L.) dan Peppermint terhadap Pertumbuhan Streptococcus mutans Lutfi Lailia Sofidiana; Erna Sulistyani; Pujiana Endah Lestari
Pustaka Kesehatan Vol 10 No 3 (2022): Volume 10 No.3, 2022
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v10i3.25232

Pegagan leaves and peppermint leaves contain antibacterial and refreshed breath so that the leaves of both these plants have the potential to be used as a mouthwash to prevent many conditions of the oral cavity including caries. One way to reduce the incidence of caries is to use mouthwash that can inhibit the growth of caries-causing bacteria, namely Streptococcus mutans. The use of chemical mouthwash many side effects. Therefore, it is necessary to innovate natural-based mouthwashes that can inhibit the growth of S. mutans with minimal side effects. This study aimed to proved the antibacterial power of the combination of pegagan leaf extract (Centella asiatica) and peppermint leaf (Mentha piperita) in inhibiting the growth of S. mutans. This study used the disc diffusion methode of 4 treatment groups (5%, 10%, 20% and 40% extract), positive control group (chlorhexidine), and negative control group (sterile akuades). Data were analyzed using Kruskal-Wallis Test and Mann-Whitney test. The combination extract of pegagan (Centella asiatica) and peppermint (Mentha piperita) has an antibacterial effect and can inhibit the growth of S. mutans.

Bioactive potential of edel cocoa bean (theobroma cacao L) from Kedaton Jember: Cytotoxicity and antioxidants evaluation Rina Sutjiati; Leliana S. Devi; Dwi Prijatmoko; Herniyati; Rudy Joealijanto; Erna Sulistyani; Bilqis P. Safitri; Nihla Fitriyani
Journal of Dentomaxillofacial Science Vol. 7 No. 1 (2022): (Available online: 1 April 2022)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v7i1.1401

Objective: Kedaton, a small village located in Jember is well known as one of the largest edel cocoa producers in the world. The cocoa beans contain nutrients and numerous beneficial properties, one of them is flavonoids, that work as antioxidants that can be utilized in dentistry, particularly orthodontics. The use of plants for the treatment must be ensured as safe without any toxic properties. Material and Methods: This study use DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical method to evaluate antioxidant properties and MTT 3-(4.5-Dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide assay to assess the fibroblast cell activity. Results: The fermented edel cocoa bean extract exhibited the IC50 value of 33.97 ppm and the unfermented edel cocoa bean extract that had the IC50 value of 9.56 ppm. Both of them have very strong antioxidant activity. Fermented edel cocoa bean extract in concentrations of 1.56%, 3.125%, 6.25%, and 12.5%; as well as unfermented edel cocoa bean extract in concentrations of 1.56% and 3.125% did not have cytotoxic effects to fibroblasts. Conclusion: The unfermented edel cocoa bean extracts have higher antioxidant activity compared to fermented ones. The fermented edel cocoa bean extracts did not have cytotoxic effect, meanwhile the unfermented edel cocoa bean extracts in concentrations of 6.25% and 12.5% have cytotoxic effect to fibroblasts.

Neem Gum (Azadirachta Indica) Solution Potential for Improving Viability of Bifidobacterium Longum and Lactobacillus acidohilus Bacteria Hamzah, Zahreni; Sulistyani, Erna; Hafizh, Cinantya; Handayani, Ari T.W.
Indonesian Journal of Life Sciences 2022: IJLS Vol 04 No .01
Publisher : Indonesia International Institute for Life Sciences

Neem gum is a liquid that comes out of the stems of neem plants (Azadirachta indica) that have hardened and become crystals. Soluble polysaccharides compounds contained in this gum are composed of several types of monosaccharides such as arabinose, fucose, galactose, glucuronic acid, glucose, mannose, and xylose. The content of neem gum has prebiotic potential so it can be used as a source of nutrients for lactic acid bacteria. Bifidobacterium longum and Lactobacillus acidophilus are lactic acid bacteria classified as beneficial bacteria in the gastrointestinal tract. The content of soluble polysaccharides in neem gum can be selectively digested by these bacteria. This study aims to determine the potential of neem gum solution (Azadirachta indica) to the viability value of B. longum and L. acidophilus bacteria. The viability of B. longum and L. acidophilus bacteria was calculated using the MTT assay method. The results showed the viability value of both bacteria exposed to neem gum solution at concentrations of 5% (b/v), 10% (b/v), and 20% (b/v) increased compared to negative controls that were not given exposure to neem gum solution. Based on the results of the study, it concluded that neem gum is effective in increasing the growth of bacteria B. longum and L. acidophilus

Uji Aktivitas Antibakteri Ekstrak Etanol Daun Ungu (Graptophyllum pictum (L) Griff) terhadap Pertumbuhan Streptococcus sanguinis Syahrani, Nafila; Kurniawati, Atik; Prihanti, Ayu Mahartini; Sulistyani, Erna; Lestari, Pujiana Endah
Pustaka Kesehatan Vol 12 No 1 (2024): Volume 12 No.1, 2024
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v12i1.42325

Streptococcus sanguinis has strong adhesion with salivary pellicles which causes other oral bacteria to attach more and has contribution in causing caries and periodontal disease. S. sanguinis mediated the adhesion of S. mutans to dental pellicle receptors so inhibiting the interaction between S. sanguinis and S. mutans would prevent the risk of dental caries. Purple leaf (Graptophyllum pictum) is one of the medicinal plants or natural ingredients that are useful as antibacterials. The purpose of this study was to examine antibacterial activity of purple leaf extract against S. sanguinis. This experimental laboratory study with a pre-posttest control group design was conducted by dilution method on treatment groups, purple leaf extract with concentrations of 25%, 12.5%, 6.25% and 3.12%. Visual and spectrophotometer examination was observed before and after incubation.The result showed that purple leaf extract with concentrations of 6.25% and 3.12% and control + groups had not turbidity and decreased absorbance value. Furthermore, the One Way Anova parametric statistical test and also Post-Hoc Least Significant Difference showed that there were significant differences between the study groups. The purple leaf extract (Graptophyllum pictum L. Griff) had antibacterial activity against S. sanguinis. The antibacterial test for extracts that are dark in color can be continued using the TVC (Total Viable Count) method to provide a quantitative estimate of the concentration of bacterial cells in the sample in units of CFU/ml.

Bay Leaf Extract Aggravated Fungal Infections in The Rat Model of Oral Candidiasis. Sulistyani, Erna; Triwahyuni, Iin Eliana; Dewi, Leni Rokhma; Futaihana, Hasna' Garaniva; Andriani, Hilma; Agustin, Merry Ayu Ayu; Khairunnisa, Luthfitah Zahra
Odonto : Dental Journal Vol 12, No 2 (2025): August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.2.136-142

Background: The prevalence of oral candidiasis has been increasing in recent years due to various factors, such as diseases that weaken the immune system, medication, xerostomia, and chemotherapy. The types of antifungal drugs are still very limited, and those that can be given systemically have significant side effects. Numerous in-vitro studies have demonstrated that bay leaves have antifungal properties. This study aimed to evaluate the effect of bay leaf extract in vivo on the oral candidiasis rat model.Method: The rat model for oral candidiasis was created by adding Tetracycline HCl 500 mg/L to the rats' drinking water. Then, the rats were injected with methylprednisolone one day before and after inoculating 0.3 ml of Candida albicans (9.4 x 107 cells/ml) into the oral cavity of the rats. The bay leaf extract was given to two groups of the oral candidiasis rat model by intragastric tube at 250 mg/kgBW/day (P1) and 500 mg/kgBW/day (P2) doses for 5 days. Oral candidiasis infection was evaluated on the last day by counting the number of colonies from swabs of the oral mucosa cultured on SDA-C. Result: After 5 days of treatment, the number of fungal colonies of group P2 was surprisingly higher than P1, positive and negative control. Conclusion: This study revealed that bay leaf extract aggravated oral candidiasis in vivo. The flavonoids in bay leaves may inhibit the production of pro-inflammatory cytokines, which could diminish the rats' immune response.

Bay Leaf Extract Aggravated Fungal Infections in The Rat Model of Oral Candidiasis. Sulistyani, Erna; Triwahyuni, Iin Eliana; Dewi, Leni Rokhma; Futaihana, Hasna' Garaniva; Andriani, Hilma; Agustin, Merry Ayu Ayu; Khairunnisa, Luthfitah Zahra
Odonto : Dental Journal Vol 12, No 2 (2025): August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.2.136-142

Background: The prevalence of oral candidiasis has been increasing in recent years due to various factors, such as diseases that weaken the immune system, medication, xerostomia, and chemotherapy. The types of antifungal drugs are still very limited, and those that can be given systemically have significant side effects. Numerous in-vitro studies have demonstrated that bay leaves have antifungal properties. This study aimed to evaluate the effect of bay leaf extract in vivo on the oral candidiasis rat model.Method: The rat model for oral candidiasis was created by adding Tetracycline HCl 500 mg/L to the rats' drinking water. Then, the rats were injected with methylprednisolone one day before and after inoculating 0.3 ml of Candida albicans (9.4 x 107 cells/ml) into the oral cavity of the rats. The bay leaf extract was given to two groups of the oral candidiasis rat model by intragastric tube at 250 mg/kgBW/day (P1) and 500 mg/kgBW/day (P2) doses for 5 days. Oral candidiasis infection was evaluated on the last day by counting the number of colonies from swabs of the oral mucosa cultured on SDA-C. Result: After 5 days of treatment, the number of fungal colonies of group P2 was surprisingly higher than P1, positive and negative control. Conclusion: This study revealed that bay leaf extract aggravated oral candidiasis in vivo. The flavonoids in bay leaves may inhibit the production of pro-inflammatory cytokines, which could diminish the rats' immune response.

Evaluation of rat model oral candidiasis mucosal response immune Sulistyani, Erna; Triwahyuni, Iin Eliana; Harmono, Happy; Sholehah, Almaratus; Prameswari, Syefira Putri; Mayangsari, Zahra; Perwitasuci, Brigitta Damai; Wulandari, Widya Nurfitriastuti
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.172-179

Background: The choice of antifungal drugs is limited and has not yet made significant progress. The animal model of oral candidiasis is a crucial factor in exploring antifungals. In our previous study's oral candidiasis rat model, we found an increase in the sedimentation rate and a decrease in infection microbiologically and histologically on day 8 compared to day five after inoculation. On the other hand, blood plasma levels of IL-17, as the main cytokine in fungal infections, keep at a low level. The study aims to prove the increase in the immune response in the oral mucosa of an oral candidiasis rat model on the 8th day after inoculation of C. albicans. Methods: The sample consisted of 2 groups; healthy Wistar rats as a control and Wistar Rat rats treated as an oral candidiasis rat model. Eight days after inoculating C. albicans,The immune response was evaluated by counting the number of visual field inflammatory cells on the dorsum of the rat dorsum tongue.Results: The number of inflammatory cells, including macrophages in the dorsal tongue mucosa of oral candidiasis rats, was higher than that of healthy rats. Conclusion: There was an increase in the number of inflammatory cells in the dorsum of the tongue mucosa of the oral candidiasis rat model on day 8, which is a sign of an increased immune response in the experimental animal model.

Evaluation of rat model oral candidiasis mucosal response immune Sulistyani, Erna; Triwahyuni, Iin Eliana; Harmono, Happy; Sholehah, Almaratus; Prameswari, Syefira Putri; Mayangsari, Zahra; Perwitasuci, Brigitta Damai; Wulandari, Widya Nurfitriastuti
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.172-179

Background: The choice of antifungal drugs is limited and has not yet made significant progress. The animal model of oral candidiasis is a crucial factor in exploring antifungals. In our previous study's oral candidiasis rat model, we found an increase in the sedimentation rate and a decrease in infection microbiologically and histologically on day 8 compared to day five after inoculation. On the other hand, blood plasma levels of IL-17, as the main cytokine in fungal infections, keep at a low level. The study aims to prove the increase in the immune response in the oral mucosa of an oral candidiasis rat model on the 8th day after inoculation of C. albicans. Methods: The sample consisted of 2 groups; healthy Wistar rats as a control and Wistar Rat rats treated as an oral candidiasis rat model. Eight days after inoculating C. albicans,The immune response was evaluated by counting the number of visual field inflammatory cells on the dorsum of the rat dorsum tongue.Results: The number of inflammatory cells, including macrophages in the dorsal tongue mucosa of oral candidiasis rats, was higher than that of healthy rats. Conclusion: There was an increase in the number of inflammatory cells in the dorsum of the tongue mucosa of the oral candidiasis rat model on day 8, which is a sign of an increased immune response in the experimental animal model.

Role of C-type Lectin Receptor to C.albicans on Promotion of Inflamantory Diseases Erna Sulistyani
Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar

In early 2010 there was a very rapid progress in the development of immunity to fungi, i.e the discovery of CLR. CLR is one of the PRR receptors found in immune cells that recognize the cell wall of fungi and then trigger a transduction signal that eventually provoke the production of various proinflamatory include IL1β, IL6, TNF α and induce polarization Th17. In the condition of infection the number of innate immune cells increases dramatically, lead to increasing of cytokines produced. Both cytokines and the cells that produce them enter the bloodstream. If there is unbalance level of pro and anti inflammatory cytokine, various inflammatory and/or autoimmune diseases can be occur. Unlike the focal concept infection, where the agent will cause disease if enters the blood vessels, through this concept a fungal infection at epithelium can lead to inflammatory diseases in other part of body without having to go through fungus

Median rhomboid glossitis as a sign of undiagnosed diabetes mellitus - a case report Sulistyani, Erna; Triwahyuni, Iin Eliana; Astuti, Pudji; Swandari, Rindang
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.7-13

Background: Median Rhomboid Glossitis (MRG) is included in the group of nodular-plaque-like candidiasis, a subtype of chronic hyperplastic candidiasis. A lot of literature suggests that MRG is a manifestation of Diabetes Mellitus (DM), but the association between DM and MRG is unclear until now. This article reports an MRG found in an undiagnosed diabetes mellitus patient. Case: In October 2019, A 71-year-old man came to RSGM Universitas Jember to fix his loose denture. In the oral examination, we found a well-bordering, atrophic oval area surrounded by thick- soft white plaque. In his leg, we found itchy papule, ulcer, and crust multiple lesions. The microscopic examination of the white plaque swab showed that the spore and hyphae were found to fill all fields of view. The direct sugar blood level test showed that the sugar blood level was 390 mg/dL. We diagnosed the patient as having an MRG associated with suspected DM. Case Management: The patient was given miconazole oral gel and recommended to use it after cleaning the tongue three times a day. For diabetes management, the patient was referred to the Unit Medical Center University of Jember. The patient was diagnosed with diabetes mellitus and given oral-antidiabetics. After two weeks, the atrophic area had improved, and the white plaque had disappeared. Conclusion: General practitioners must be aware if they find the MRG in their patients caused a probability of underlying undiagnosed systemic disease, particularly diabetes mellitus on them.

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