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All Journal e-Journal Pustaka Kesehatan Pharmaciana: Jurnal Kefarmasian UNEJ e-Proceeding AcTion: Aceh Nutrition Journal Jurnal Kimia Terapan Indonesia Indonesian Journal of Chemistry Chemistry Indonesian Journal of Chemical Research JFIOnline Pharmaceutical Sciences and Research (PSR) Jurnal Natural Jurnal Tumbuhan Obat Indonesia

Yuni Retnaningtyas

Fakultas Farmasi Univesitas Jember

Author-ID : 2973855

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health

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Pengujian Aktivitas Antioksidan Kombinasi Ekstrak Metanol Daun Mangga Gadung (Mangifera indica L. var. gadung) dan Ekstrak Etanol Daun Pandan Wangi (Pandanus amaryllifolius Roxb.) (Antioxidant Activity Assay of Methanolic Extract of Gadung Mango Leaves (Mangifera indica L. var. gadung) and Ethanolic Extract of Pandan Leaves (Pandanus amaryllifolius Roxb.) Combination) Dewi Kusumaningrum Pamungkas; Yuni Retnaningtyas; Lestyo Wulandari
Pustaka Kesehatan Vol 5 No 1 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i1.3949

The content of polyphenolic compounds in mango and fragrant pandan leaves has been showed an antioxidant activity and commonly used as traditional treatment of some diseases. The aim of this research was to determine the activity of antioxidant of the gadung mango leaves and fragrant pandan leaves extract combination. The activity of antioxidant was investigated by DPPH method (IC50) with vitamin C as positive control. The results showed that the sample had lower antioxidant activities than vitamin C. The IC50 value of vitamin C was 2.613±0.021 μg/ml, followed by methanolic extract of gadung mango leaves, ratio 1:1 of gadung mango leaves extract and pandan leaves extract combination, and ethanolic extract of fragrant pandan leaves with IC50 value of 3.263±0.009; 13.392±0.157; 39.700±1.003 μg/ml, respectively. Methanolic extract of gadung mango leaves and ethanolic of fragrant pandan leaves combination didn't have higher antioxidant activities compared to the antioxidant activities of the singular methanolic extract of gadung mango leaves itself. Keywords: antioxidant activity, gadung mango, fragrant pandan

Inulin determination of yam bean tuber (Pacyrrhizus erosus l.) water extract from different altitude areas using TLC- Densitometry Yuni Retnaningtyas; Lestyo Wulandari; Mariska Wimala
Pharmaciana Vol 12, No 1 (2022): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Inulin is a polysaccharide with a lot of potential in the field of food and pharmaceuticals. Inulin can be found in yam bean tuber. Chemical constituents of yam bean tuber are inulin, pachyrion, and rotenone. The levels of inulin could be influenced by differences in the altitude of the cultivation area. The objective of this study was to determine the inulin of yam bean tuber extract from several areas in East Java based on the altitude difference, they are low (Gresik), middle (Kediri), and high (Malang), using validated TLC Densitometry method. The samples were extracted by the infusion method. The samples were put to silica gel TLC plates and developed using ascending chromatography with a mobile phase of 0.5:7:2 glacial acetic acid: methanol: Â deionized water. Â Densitometry at 380 nm was used to assess the intensity of inulin spots dipped in Aniline: diphenylamine: phosphoric acid (5:5:1), and winCATS software version 1.3.0 was used to quantify the results. A standard curve Â meets the requirement of linearity (r=0.997; Vxo=3.847%), sensitivity (LOD=71.030 ng/spot, and LOQ=236.766 ng/spot), selectivity and specificity, Precision (RSDâ‰¤2.754 %), and accurate (% recovery Â± RSD = 100.161 Â± 1.839%). The results showed that the inulin content in yam tubers produced by areas with different altitudes had different inulin levels. The levels of inulin derived from the low altitude (Gresik) was 9.066 Â± 1.218 %, middle (Kediri) was 7.776 Â± 0.420 %, and high (Malang) was 6.796 Â± 2.045 %. One-Way ANOVA test showed the significant difference between the levels of inulin from different altitude areas (p < 0.01), the highest inulin level comes from yam bean tuber from low altitude.Â

STUDY OF ANTIOXIDANT ACTIVITY COMBINATION OF ARABICA COFFEE LEAF ETHANOL EXTRACT AND ROSELLE FLOWER PETAL WATER EXTRACT Yuni Retnaningtyas; Yodi Setiadi
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Free radicals are molecules that have one or moreunpaired electrons. As we know that somecardiovascular diseases such as heart disease,diabetes mellitus, and cancer are diseases thattriggered by oxidative damage in the presence offree radicals. Excessive oxidation of the nucleicacids, proteins, fats and DNA cells can initiate theoccurrence of degenerative diseases such ascoronary heart disease, cataracts, cognitivedisorders and cancer (Leong and Shui, 2001; Pietta1999). Humans have had a defense system againstoxidants that come from within or from outside thebody in the form of diet. Defense of the body suchas enzymes peroxidase, catalase, glutathione,histidine, peptidin often still lacking due toenvironmental influences and poor diet (Pietta,1999).Antioxidants are molecules that can inhibit theoxidation of molecules that can produce freeradicals. Antioxidant is a compound that has theability to react with free radicals to produce a stablefree radical by accepting or donating electrons.Based on the source of, antioxidant divided into twokinds, namely natural antioxidants and antioxidantssynthetic (artificial). Synthetic antioxidants are mostoften used such as Propyl Error (PG), Butylatedhydroxyanisole (BHA), Butylated hydroxytoluene(BHT) and Tertbutylhydroquinone (TBHQ). Thesynthetic antioxidants can give side effects thatharmful to human health because it’s carcinogenic.Various studies on BHA and BHT indicate that thiscan cause tumors in laboratory animals at long-termuse (Kikuzaki, 1993). Natural antioxidant is anantioxidant that is obtained naturally in plants.Natural antioxidants are usually more desirable,because the level of better security and broaderbenefits in the food, health and cosmetics. Naturalantioxidants can be found in food of vegetables andfruits. As an example of natural antioxidants such asVitamin A, Vitamin C, Vitamin E, and Polyphenols.Their concerns about the potential side effects ofsynthetic antioxidants used as motivation for thestudy of natural antioxidants as one alternative.According to previous studies, plants with highphenol levels have high antioxidant activity as well,this is because most of the antioxidant compoundsare derivatives of phenol. Coffee is one agriculturalcommodity in Indonesia. In the Indonesian marketencountered two types of coffee, the Arabica coffee(Coffea arabica) and Robusta coffee (Coffeacanephora). Previous research showed that theleaves of the Arabica coffee (Coffea arabica)contains a lot of polyphenol compounds areefficacious as an antioxidant. Research conductedshows the results of a very high antioxidant activity(Pellegrini et al., 2003).Nowdays roselle (Hibiscus sabdariffa L.) become sopopular. Almost in every exhibition of medicinalplants, roselle is always introduced. This is due toalmost all parts of this plant can be used fortreatment, particularly for alternative medicine.Roselle contain chemical compounds that canprovide many benefits. According to the results ofprevious studies showing that the anthocyanincontent in the plant is effective in counteractingfree radicals cause cancer and other diseases(Mardiah et al., 2009).This research will be conducted to determine theantioxidant activity of ethanol extract of the leavesof the Arabica coffee (Coffea arabica) and waterextract of roselle calyx (Hibiscus sabdairffa) byDPPH (1,1-diphenyl-2-picrylhydrazyl) because themethod this is the simplest method, fast, easy,accurate, inexpensive, and capable of measuringthe various components that act as free radical orhydrogen donor. In addition DPPH method does notrequire a lot of reagents such as another method ofmeasuring antioxidant activity. Comparativecompounds used in measuring the activity of theseantioxidants are Vitamin C or ascorbic acid. Used asa comparison compound for the preparationVitamin C is widely used in the market and has beenshown to have antioxidant activity and relativelycheap. According to the study, Vitamin C is used bymany researchers as a benchmark test for theantioxidant activity

PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI LAPIS TIPIS DENSITOMETRI UNTUK PENETAPAN KADAR TEOFILIN DAN EFEDRIN HIDROKLORIDA SECARA SIMULTAN PADA SEDIAAN TABLET Lestyo Wulandari; Yuni Retnaningtyas; Diyanul Mustafidah
Jurnal Kimia Terapan Indonesia Vol 15, No 1 (2013)
Publisher : Research Center for Chemistry - LIPI

The combination of Theophylline and Ephedrine isoften used to induce an additive effect in alleviating thesymptoms of respiratory disorders. This research is aimed todevelop a Thin Layer Chromatography (TLC) densitometrymethodfor the simultaneous determination of Theophyllineand Ephedrine hydrochloride in tablet dosage form. Themethod employed TLC aluminium plates precoated withsilica gel 60F254 as the stationary phase and the mixture ofethyl acetate: glacial acetic acid: aquabidest (11: 5 : 1 v/v)asthe eluen system. For detection of Ephedrinehydrochloride, the TLC plate must be re-eluted with 0,5%ninhydrin solution in aquabidest, and then heated at 105Â°Cfor 15 min. Quantitative evaluation was performed bymeasuring the absorbance-reflectance of the analyte spot at279 nm and 505 nm for Theophylline and Ephedrinehydrochloride, respectively. validation of the method showthat TLC Densitometryis selective and specific,showed agood linearity, precise, and accurate and can be used forroutine analysis of tablet in industrial quality controllaboratories.Keywords:TLCDensitometryV, alidationT, heophyllineE, phedrinehydrochloride

DEVELOPMENT AND VALIDATION OF A TLC DENSITOMETRIC METHOD FOR DETERMINATION OF GLIMEPIRIDE IN TABLETS Yuni Retnaningtyas; Lestyo Wulandiri; Gabriella F Punu
Jurnal Kimia Terapan Indonesia Vol 16, No 1 (2014)
Publisher : Research Center for Chemistry - LIPI

A simple and valid TLC method has been developed for the determination of glimepiride in tablet formulation. After extraction of the analyte with a mixture of methanol and ammonia 0,2M (1:1, v/v), the extracts were spotted on precoated TLC silica gel F254 plates, which were developed with a mixture of toluene:methanol:ethyl acetate (75:20:5, v/v/v). Quantitative evaluation was performed by measuring the absorbance reflectance of the analyte spots at 238 nm. The method was validated for specificity, linearity, accuracy and precision. Good linearity was achieved in the concentration range 100â€“800 ng/spot. The RSD of repeatability and intermediate precision were found to be less than 2%, whereas the mean of the recovery data was 100-101%. The detection limit and quantification limit were 22 and 74 ng/spot, respectively. The method is specific, linear, precise, and accurate; it can be used for the routine quality control testing of marketed formulations.Keywords: glimepiride, TLC densitometric, validation of pharmaceutical methods, pharmaceutical analysis, antidiabetic drugÂ Sebuah metode Kromatografi Lapis Tipis (KLT) yang sederhana dan valid telah dikembangkan untuk penentuan glimepiride dalam sediaan tablet. Setelah analit dalam sampel diekstraksi dengan campuran metanol dan amonia 0,2 M (1:1,v/v), ekstrak yang terlihat pada lempeng silika gel F254, yang dikembangkan dengan campuran toluen : metanol : etil asetat (75:20:5, v/v/v). Evaluasi kuantitatif dilakukan dengan mengukur reflektansi absorbansi noda analit pada panjang gelombang 238 nm.Metode ini divalidasi meliputi spesifisitas, linearitas, akurasi dan presisi.Linearitas yang baik dicapai pada rentang konsentrasi 100-800 ng / spot. RSD pengulangan dan presisi intermediate menunjukkan nilai kurang dari 2 %, sedangkan rata-rata data recovery adalah 100-101 % .Batas deteksi dan batas kuantifikasi adalah 22 dan 74 ng / noda.Metode ini spesifik, linear, tepat, dan akurat, bisa digunakan untuk pengujian kontrol kualitas rutin tablet glimepirid dipasarkan. Kata Kunci: glimepiride, TLC densitometric, validation of pharmaceutical methods, pharmaceutical analysis, antidiabetic drug

DETERMINATION OF INULIN FROM MULTIVITAMIN SYRUP PRODUCT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH RI DETECTOR Yuni Retnaningtyas
Indonesian Journal of Chemistry Vol 12, No 2 (2012)
Publisher : Universitas Gadjah Mada

At present, inulin is often added to multivitamin syrup product. The determination of the component of preparation both qualitatively and quantitatively is important to ensure quality of the product. This research is aimed to develop a high performance liquid chromatography method to analyze inulin in multivitamin syrup preparation. Separation of inulin from the sample, was performed using Aminex column HPX-87H (300 x 7.8 mm) Ion Exclusion at a temperature of 80 °C with isocratic elution system using deionized water as mobile phase at a flow rate of 0.5 mL/min, and detected by using refractive index detector. This method validation showed a good linearity with correlation coefficient (r) of 0.999 while the coefficient of variation of the regression function (Vx0) was 2.00%. The limit of detection (LOD) and the limit of quantification (LOQ) of the method were respectively 0.12 mg/mL and 0.37 mg/mL. The mean absolute recovery of inulin from the simulation sample was 99.42% and the method precision was less than 2%. The proposed method has been applied to the determination of inulin in commercial multivitamin syrup and the recovery of label claim was 99.9 mg/100 mL. The proposed HPLC method is rapid, simple, and selective for routine analysis.

Identification of Candida species in faeces of children with Autism Spectrum Disorder (ASD) carbohydrate and noncarbohydrates diets Yuni Retnaningtyas; Dewi Dianasari; Luna Ivanka Dwi Elavia; Ulfatul Munawaroh
AcTion: Aceh Nutrition Journal Vol 7, No 2 (2022): November
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v7i2.746

Increased colonization of Candida, especially C. albicans can occur in children with ASD and cause the severity of symptoms. One of the triggers for increased colonization in ASD children is the consumption of carbohydrates. This study aimed to identify Candida species in the faeces of ASD children who diet and nondiet carbohydrates from schools with special needs in Jember, East Java. The research design is a true experimental laboratory. The sample consisted of 26 ASD children and 13 healthy children as controls, with different subjects calculated using the Krejcie and Morgan formula. The test samples were identified for fungi by examining germ tube morphology, chlamydospores, and Candida species and compared with controls. The results showed differences in Candida species in the two test groups. Species C. albicans, C. dubliniensis, and C. tropicalis were found in almost all the faeces of ASD children with noncarbohydrates diet. The species C. parapsilosis, and C. albicans were found in a small proportion of the faeces of ASD children's carbohydrate diet. In contrast, the species found in the control group were C. albicans, C. dubliniensis, C. parapsilosis, and C. glabrata. In conclusion, carbohydrate consumption can affect the Candida species in the faeces of children with ASD.

AKTIVITAS ANTIOKSIDAN FRAKSI AIR DAN FRAKSI ETER KOMBINASI EKSTRAK METANOL DAUN KOPI ARABIKA (Coffea arabica L.) DAN KULIT BATANG KAYU MANIS (Cinnamomum burmannii Nees ex BI.): Antioxidant Activity of Water and Ether Fraction Combination of Arabica Coffee Leaves (Coffea arabica L.) Extract and Cinnamon burmanii Bark (Cinnamomum burmannii Nees ex Bi.) Retnaningtyas, Yuni; Wulandari, Lestyo; Fauzan Arrozi
Jurnal Tumbuhan Obat Indonesia Vol. 16 No. 1 (2023): July 2023
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v16i1.604

Antioxidants are compounds that can inhibit an oxidation reaction, by binding to free radicals that play a role in the emergence of several degenerative diseases. Arabica coffee (Coffea arabica) and cinnamon (Cinnamomum burmannii) are plants that have antioxidant activity so that when combined they have the potential to produce a synergistic effect. The purpose of this study was to test the antioxidant effects of the combination of methanol extract of Arabica coffee leaves and cinnamon bark in both the water and ether fractions. Antioxidant activity was determined using the DPPH method, as a positive control vitamin C was used, and antioxidant activity was expressed in the form of IC50. The results showed a synergistic effect of the two extracts where the antioxidant activity of both the water fraction and the ether fraction in the combined form was greater than the single form. The highest antioxidant activity was shown by the water fraction of the combination of methanol extract of Arabica coffee leaves and cinnamon bark in a ratio (1:2) with an IC50 value of 8.759 ± 0.050 g/mL. This antioxidant activity is lower than the antioxidant activity of vitamin C with an IC50 value of 3.267 ± 0.007 g/mL

Stability Indicating RP-HPLC for Quantification Mangiferin in Extract of Three Species Mango Leaves Retnaningtyas, Yuni; Kristiningrum, Nia; Renggani, Hidayah Dwi; Sary, Indah Purnama
Indonesian Journal of Chemical Research Vol 8 No 1 (2020): Edisi Bulan Mei (Edition for May)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2020.8-yun

The stability indication of Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) method was validated for quantitative determination of mangiferin on three species mango leaves (Mangifera odorata Griff, Mangifera foetida Lour, and Mangifera indica L.). The samples were extracted by maseration method using methanol and concentrated using rotary evaporator. The method carried out on stationary phase a purospher RP-18 endcapped (25 cm Ã— 4.6 mm i.d., 5 Âµm) column with a mobile phase consisting of methanol: phosphoric acid 0.1% (v/v) (31:69); flow rate:0.8 mL/min; solvent methanol, detection was carried out at 258 nm. The analytical performace this measurement is good with the value of linearity (r2=0.998), precision (%RSD=0.649%), and accuration (10.67%). The forced degradation studies were carried out according to the International Conference on Harmonization (ICH) guidelines. The results indicating that the complete separation between degradation products and mangiferin peak occured. The degradation limit of mangiferin 5â€“20% (according to the guideline of ICH) except in basic condition (100%). The method was succesful applied to determine of the mangiferin in pakel (Mangifera foetida), kweni (Mangifera indica) and kopyor (Mangifera odorata) extract. The mangiferin content was obtained are pakel (9.95%), kopyor (7.40%) and kweni (Mangifera odorata) (2.49%) respectively.

Biosynthesis of AgNPs Mediated by Equisetum debile Roxb. Herb Water Extract and Its Potency as an Antioxidant Retnaningtyas, Yuni; Karmilasari, Alviani Dwi; Puspitasari, Endah
Pharmaceutical Sciences and Research Vol. 11, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Silver nanoparticles (AgNPs) are metal nanoparticles currently under development. AgNPs are recognized for their remarkable stability, low reactivity, and advantageous biological properties, including antioxidant activity. Phenolic compounds derived from the Equisetum debile Roxb. (Ed-R) herb have the potential to be explored as bio-reduction and capping agents in the synthesis of silver nanoparticles. The primary objective of this study is to synthesize AgNPs using a water extract from Ed-R herb and to assess their antioxidant activity. The optimal composition for the biosynthesis of AgNPs comprises 10% Ed-R water extract, 8.0 mM AgNO3, and deionized water in a ratio of 0.5:20:12 (v/v/v), with the mixture incubated for 60 minutes at 50°C. AgNPs were characterized by conducted using UV-Vis Spectrophotometry, FT-IR, Scanning SEM, and PSA. The total phenolic content (TPC) of the Ed-R herb water extract at the optimal concentration (10%) was determined to be 61.503 ± 0.089 mg GAE 100 mL-1. The characterization results indicated that the AgNPs exhibited a spherical morphology, with a particle size of 67 nm, a polydispersity index (PDI) of 0.424, a maximum wavelength of 423 nm, and an absorbance value of 1.397. The antioxidant activity of AgNPs was evaluated using the DPPH method, resulting in an IC50 value of 76.604 ± 0.846 μg mL-1, which signifies strong antioxidant activity. These findings suggest that the water extract from the Ed-R herb can effectively act as both bioreduction and capping agent in the synthesis of AgNPs, which demonstrates significant antioxidant properties.

Co-Authors . Ifada Aida, Siti Nor Annisa Rahmawati Arrofita Ani Sandiya Ayik Rosita Puspaningtyas1 Bambang Kuswandi CRISTIANTY, FRANSISKA MARIA Dewi Dianasari Dewi Kusumaningrum Pamungkas Diyanul Mustafidah Endah Puspitasari Fauzan Arrozi Gabriella F Punu Hamzah, Muhammad Hafidi Hamzah, Muhammad Hafidi Hilmia Lukman, Hilmia Ika Oktavianawati Indah Purnama Sary, Indah Purnama Karmilasari, Alviani Dwi Khoirun Nisa' Lestyo Wulandari Lestyo Wulandari Lestyo Wulandiri Luna Ivanka Dwi Elavia Mariska Wimala Marizka Wimala Nia Kristiningrum Renggani, Hidayah Dwi Risa Wahyu Ananda Ulfatul Munawaroh WARDANI, PRAMUDIA Yodi Setiadi

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