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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Global Medical and Health Communication LENSA (Lentera Sains): Jurnal Pendidikan IPA Indonesian Chimica Letters Life Science and Biotechnology
Esti Utarti
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Education Environmental Science Health Professions Immunology & microbiology Mathematics Nursing Physics Public Health

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Growth Pattern and Degradation Activity of Caffeine-degrading Bacteria Consortium Suksma, Nadhea Ayu; Utarti, Esti; Arimurti, Sattya
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.32609

Abstract

Caffeine-degrading bacteria can be used as agents to degrade caffeine, thereby reducing the concentration of caffeine in organic waste. The decomposition process is carried out by a single bacterium or a consortium of bacteria. Caffeine-degrading bacteria from Sempol, Bondowoso, namely Acinetobacter gerneri KAFS 47, Paracoccus denitrificans KAFS 16 and Pseudomonas plecoglossicida KAFS 34, could be used as a bacterial consortium to promote caffeine degradation. The aim of this study was to analyze associations between caffeine-degrading bacteria isolates, bacterial resistance to antibiotics, growth patterns, and caffeine degradation of a consortium of caffeine-degrading bacteria, and the correlation of bacterial growth with caffeine degradation. The research method used is an analysis of the association between isolates, the development of bacterial consortium growth patterns, and their analysis based on antibiotic resistance, patterning of caffeine degradation, and correlation test (Pearson) of bacterial growth with caffeine degradation. The result of the association test between bacteria showed that the three bacteria had the potential to be used as a consortium of caffeine-degrading bacteria. A. Gerneri, P. denitrificans, and P. plecoglossicida were resistant to the antibiotic cefixime (100 ppm), erythromycin (50 ppm), lincomycin (50 ppm), metronidazole (50 ppm), and sanprima (50 ppm). The growth of the bacterial consortium (54.779 CFU/mL) was higher than that of P. plecoglossicida (49.277 CFU/mL) and lower than that of A. gerneri (93.481 CFU/mL) and P. denitrificans (84.940 CFU/mL) in incubation time of 4 days. However, the consortium of bacteria and P. plecoglossicida were able to degrade caffeine 24 hours faster (3 days) than the other two single isolates (4 days) to degrade 2.5 g/L caffeine in media to 0%. Bacterial growth due to caffeine degradation has a perfect correlation value (>0. 950) and is negative.
Using Lignosellulose Waste as a Xylanase Production Media of Mold Isolated from Rice Straw of Coastal-field Utarti, Esti; Siswanto, S.
Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (625.534 KB) | DOI: 10.19184/jid.v19i2.7007

Abstract

Hemicellulose is one of lignocellulose waste component, so that xylanase is one of importance enzyme of lignocellulose waste biodegradation. Molds as main decomposer lignosellulose waste has enzyme activities higher than yeast and bacteria. The aim of the research is to find mold that have xylanolitic activity using lignocellulose waste as media production. The research consist of isolations and screening mols from coastal-field of watu Ulo Jember, xylanase production using lignocellulose waste and idntification of mold which has the highes xylanase activity. A total of 66 molds isolated from rice straw in coastal-field of Watu Ulo Jember. There were screened for their xylanase activity. In semiquantitatively screen on Oat Spelt Xylan plate, the result showed that 62 have xilanolytic activities. Based on clearing zone production, isolates ESW A1 (3.2), ESW A5 (3.1), ESW C 16 (3.26), ESW D4 (3.0) and ESW D15 (3.21) have xilanase activity index higher than others. Furthermore, quantitative analysis using wheat bran, rice straw and baggase in basic salt Mandel’s modification media showed that xylanase activity of isolate ESW D4 was higher on rice straw 3% as substrate production with activity 2.66 U/mL. Isolate ESW D4 identified as Aspergillus foetidus so that called as Aspergillus foetidus ESW D4. Keywords: rice straw, coastal-field, Aspergillus foetidus ESW-D
Identifikasi Aktinomiset Selulolitik dan Xilanolitik Indigenous Utarti, Esti; Suwanto, Antonius; Suhartono, Maggy T; Meryandini, Anja
BERKALA SAINSTEK Vol 8 No 1 (2020)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v8i1.15941

Abstract

Lignoselulosa merupakan penyusun utama dinding sel tumbuhan, sehingga keberadaannya berlimpah di alam. Aktinomiset indigenous yang memiliki aktivitas selulolitik dan xilanolitik ekstraseluler berpeluang sebagai agens biokonversi limbah berlignoselulosa menjadi produk bermanfaat. Penelitian ini bertujuan untuk mendapatkan aktinomiset potensial yang memiliki aktivitas selulolitik dan xilanolitik. Penelitian ini diawali dari isolasi dan pemurnian aktinomiset indigenous asal lahan perkebunan kelapa sawit dan Taman Nasional Bukit Duabelas Jambi.Tahapan selanjutnya adalah penapisan aktivitas selulolitik dan xilanolitik dari aktinomiset, uji pertumbuhan aktinomiset pada mikrokristalin selulosa, dan identifikasi aktinomiset potensial berdasarkan karakter morfologi, fisiologi dan biokimia. Hasil penelitian menunjukkan bahwa aktinomiset isolat S2 yang diisolasi dari lahan perkebunan kelapa sawit mempunyai aktivitas selulolitik dan xilanolitik lebih baik dari keempat isolat lain. Aktinomiset isolat S2 juga mampu tumbuh secara lebih baik pada mikrokristalin selulosa. Aktivitas selulolitik, xilanolitik dan kemampuan tumbuh pada mikrokristalin selulosa dari aktinomiset isolat S2 menunjukkan potensinya sebagai agens pendegradasi material belignoselulosa. Berdasarkan identifikasi morfologi, fisiologi dan biokimia, aktinomiset isolat S2 tergolong dalam genus Streptomyces.
TRANSFORMASI GEN SOSPS1 PADA TANAMAN TEBU OVEREKSPRESI GEN SOSUT1 EVENT 2 MENGGUNAKAN AGROBACTERIUM TUMEFACIENS Media Ningtyas, Rinda; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 3 No 1 (2015)
Publisher : Universitas Jember

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Abstract

Gen SoSPS1 (Saccharum officinarum sucrose phosphate synthase 1) merupakan gen pengkode enzim SPS yang berperandalam biosintesis sukrosa pada organ fotosintesis. Gen SoSUT1 (Saccharum officinarum sucrose transporter1) merupakangen pengkode protein SUT1 yang berperan pada proses transportasi sukrosa dari organ fotosintesis (source) ke organnonfotosintesis (sink). Tujuan penelitian ini adalah untuk mendapatkan tanaman tebu overekspresi ganda yaitu gen SoSPS1dan gen SoSUT1 melalui transformasi gen SoSPS1 pada tanaman tebu overekspresi gen SoSUT1 menggunakan A.tumefaciens. Hasil analisis PCR diperoleh tanaman tebu yang positif overekspresi ganda gen SoSUT1 dan gen SoSPS1sebanyak 4 tanaman pada transformasi ke-1, 3 tanaman pada transformasi ke-2 dan 4 tanaman pada transformasi ke-3.Efektivitas rata- rata transformasi gen SoSPS1 menggunakan A. tumefaciens yang mengandung konstruk plasmid pCL4-SoSPS1 dan dikendalikan oleh promoter RUBQ2 pada tanaman tebu overekspresi gen SoSUT1 sebesar 4,59%.
Growth of Lactobacillus casei FNCC0900 in Media Based Umbi Porang Plant (Amorphophallus muelleri BI.) Azhari, Fitri; Winarsa, Rudju; Siswanto, Siswanto; Muzakhar, Kahar; Utarti, Esti; Sutoyo, Sutoyo; Arimurti, Sattya
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.19034

Abstract

Porang tuber (Amorphophallus muellerii BI.) Is a type of tuber that has a high enough glucomannan content of 67%. Glucomannan is very difficult to digest by humans directly so it takes the role of probiotics. L. casei bacteria FNCC0900 as a probiotic agent capable of utilizing glucomannan as a carbon source for growth. The purpose of this study was to determine the growth pattern and changes in environmental factors, namely the pH value of the probiotic bacteria L. casei FNCC0900 growth medium. The parameters in this study consisted of the highest cell density, generation time and pH value changes in Glucose Yeast Peptone Liquid Media, Porang Boiled Water Media and Porang Flour Liquid Media using the drop plate method which had 4 repeated calculations. Porang Boiled Water Liquid Media has a faster log phase period with a higher cell density than Porang Flour Liquid Media, but the shortest generation time is found in Porang Flour Liquid Media with the highest number of generations. L. casei FNCC0900 bacteria are more able to reduce the pH of Glucose Yeast Peptone Liquid Media compared to porang tuber-based media, so in this case L. casei FNCC0900 can be stated to be able to grow on porang tuber-based media with growth patterns, generation time, cell density and pH value. which varies.
TRANSFORMASI GEN SOSUT1 PADA TANAMAN TEBU MENGGUNAKAN AGROBACTERIUM TUMEFACIENS STRAIN GV 3101 DAN PANGKAL TUNAS TEBU IN VITRO Edia, Edia F.D; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

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Abstract

Gen SoSUT1 merupakan gen pengkode protein sucrose transporter yang memfasilitasi proses transpotasi sukrosa dari jaringan fotosintetik (source) ke jaringan pengguna (sink) pada tanaman tebu. Tujuan penelitian ini adalah mendapatkan tanaman tebu transforman melalui transformasi genetik menggunakan vektor Agrobacterium tumefaciens yang membawa gen SoSUT1. Eksplan pangkal tunas tebu in vitro diinfeksi dengan A. tumefaciens yang membawa konstruk pAct-SoSUT1 dan dilakukan seleksi pada media MS dengan penambahan antibiotik hygromycin.. Tanaman putatif transforman yang telah berhasil melewati proses seleksi dan diaklimatisasi, dilakukan isolasi DNA genom kemudian dianalisis PCR. Hasil analisis PCR dengan pasangan primer 1F/1R hpt II menunjukkan bahwa dari 24 tanaman tebu putatif transforman, didapatkan 15 tanaman tebu positif mengandung gen SoSUT1. Efektifitas rata-rata transformasi gen SoSUT1 menggunakan eksplan pangkal tunas tebu in vitro sebesar 6,8%. Kata Kunci: Agrobacterium tumefaciens, SoSUT1, sukrosa.
Partial Analysis of Probiotic Character of Lactic Acid Bacteria from the Intestines of Broilers Supplemented with Fish Protein Hydrolysate (FPH) Nashrullah, Shafa; Utami, Eva Tyas; Purniasari, Fina Yunita; Anggitasari, Dhanti Fatma; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53701

Abstract

This study aims to analyze partial characteristics of probiotics lactic acid bacteria (LAB) from broiler intestines supplemented with fish protein hydrolysate (FPH) 2% (v/w) in feed. A total of 17 LAB isolates were successfully isolated and characterized morphologically and biochemically (catalase test). The isolate showed diverse morphological and biochemical properties, obtained as many as seven isolates that met the partial characteristics of probiotics, namely U10, U11, U13, U16, U31, U33, and U37 which were Gram-positive and no endospores and catalase formations were found. The tolerance test for acid pH and NaCl performed on the seven BAL isolates showed a diverse survival rate with U11 showing the highest tolerance at pH 2 (11.4%) and NaCl concentration up to 8% (32.4%). These findings highlight seven LAB isolates with partial probiotic properties, providing new insights into the development of probiotic candidates from broiler guts to improve gastrointestinal health and productivity.
Production and Characterization od Xylanase from Actinomyces ATG 70 Using CornCob Xylan Substrate Farrennina, Tasya Preira; Putri, Amelia Fahreza; Arimurti, Sattya; Winarsa, Rudju; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53702

Abstract

Xilan is a polysaccharide that can be used as a substrate for producing xylanase. Xylan can be obtained from processing xylan-rich agricultural waste such as corn cobs, which contain 12.4% xylan. Xilan can be hydrolyzed into xylose and xylooligosaccharide (XOS) using the enzyme Xilanase. Actinomycetes have dominant xylanolytic species, Actinomyces ATG 70 has semi-qualitative xylanolytic ability with an enzyme activity index of 3.21±0.55 in xylan media. This study was conducted by extracting xylan from corn cobs, rejuvenating Actinomyces ATG 70, preparing the inoculum, creating a standard curve, producing xylanase with pH optimization of the medium, testing xylanase activity, characterizing the pH and temperature of crude xylanase, and identifying the morphology and biochemical characteristics of Actinomyces ATG 70. Optimization of the pH of the xylanase production medium was achieved at pH 8, with xylanase activity of 10.07±0.13 U/mL. The crude xylanase was characterized for pH and temperature effects, and the optimal pH for crude xylanase was found to be pH 6 with xylanase activity of 7.64±0.66 U/mL, and the optimal temperature was 50°C with xylanase activity of 11.17±0.33 U/mL. The identification results showed that Actinomyces ATG 70 belongs to Gram-positive bacteria, with a positive catalase test, white colonies, cream-colored aerial mycelium, and cream-colored pigmentation. The spore structure consists of long chains of conidia, streptococcus-shaped cells, and can form aerial mycelium, thus the isolate belongs to the genus Streptomyces.
Co-Authors AA. Istri Ratnadewi Anggitasari, Dhanti Fatma Anja Meryandini Antonius Suwanto Azhari, Fitri Bambang Sugiharto Dina Amalia Syahidah Dwi Setyati Edia F.D Edia, Edia F.D Eva Tyas Utami Evi Umayah Ulfa Farrennina, Tasya Preira Ferymon Mahulette Ida Ayu Putu Sri Widnyani Izzay Afkarina Jefri Nur Hidayat Kahar Muzakhar Kartika Senjarini Lina Nurita Maggy T Suhartono Media Ningtyas, Rinda Nashrullah, Shafa Purniasari, Fina Yunita Putri, Amelia Fahreza Riki Juni Krismiadi S Siswanto S Sutoyo S. Siswanto Saniyah Fatkhul Alim Sattya Arimurti Sattya Arimurti Siswanto Siswanto Siswanto Siswanto Suksma, Nadhea Ayu Sutoyo Sutoyo Sutoyo Sutoyo Su’udah Hasanah Tantri Raras Ayuningtyas Tri Santi Kurnia Winarsa, Rudju Wuryanti Handayani