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TRANSFORMASI GEN SOSPS1 PADA TANAMAN TEBU OVEREKSPRESI GEN SOSUT1 EVENT 2 MENGGUNAKAN AGROBACTERIUM TUMEFACIENS Media Ningtyas, Rinda; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 3 No 1 (2015)
Publisher : Universitas Jember

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Gen SoSPS1 (Saccharum officinarum sucrose phosphate synthase 1) merupakan gen pengkode enzim SPS yang berperandalam biosintesis sukrosa pada organ fotosintesis. Gen SoSUT1 (Saccharum officinarum sucrose transporter1) merupakangen pengkode protein SUT1 yang berperan pada proses transportasi sukrosa dari organ fotosintesis (source) ke organnonfotosintesis (sink). Tujuan penelitian ini adalah untuk mendapatkan tanaman tebu overekspresi ganda yaitu gen SoSPS1dan gen SoSUT1 melalui transformasi gen SoSPS1 pada tanaman tebu overekspresi gen SoSUT1 menggunakan A.tumefaciens. Hasil analisis PCR diperoleh tanaman tebu yang positif overekspresi ganda gen SoSUT1 dan gen SoSPS1sebanyak 4 tanaman pada transformasi ke-1, 3 tanaman pada transformasi ke-2 dan 4 tanaman pada transformasi ke-3.Efektivitas rata- rata transformasi gen SoSPS1 menggunakan A. tumefaciens yang mengandung konstruk plasmid pCL4-SoSPS1 dan dikendalikan oleh promoter RUBQ2 pada tanaman tebu overekspresi gen SoSUT1 sebesar 4,59%.

Growth of Lactobacillus casei FNCC0900 in Media Based Umbi Porang Plant (Amorphophallus muelleri BI.) Azhari, Fitri; Winarsa, Rudju; Siswanto, Siswanto; Muzakhar, Kahar; Utarti, Esti; Sutoyo, Sutoyo; Arimurti, Sattya
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.19034

Porang tuber (Amorphophallus muellerii BI.) Is a type of tuber that has a high enough glucomannan content of 67%. Glucomannan is very difficult to digest by humans directly so it takes the role of probiotics. L. casei bacteria FNCC0900 as a probiotic agent capable of utilizing glucomannan as a carbon source for growth. The purpose of this study was to determine the growth pattern and changes in environmental factors, namely the pH value of the probiotic bacteria L. casei FNCC0900 growth medium. The parameters in this study consisted of the highest cell density, generation time and pH value changes in Glucose Yeast Peptone Liquid Media, Porang Boiled Water Media and Porang Flour Liquid Media using the drop plate method which had 4 repeated calculations. Porang Boiled Water Liquid Media has a faster log phase period with a higher cell density than Porang Flour Liquid Media, but the shortest generation time is found in Porang Flour Liquid Media with the highest number of generations. L. casei FNCC0900 bacteria are more able to reduce the pH of Glucose Yeast Peptone Liquid Media compared to porang tuber-based media, so in this case L. casei FNCC0900 can be stated to be able to grow on porang tuber-based media with growth patterns, generation time, cell density and pH value. which varies.

TRANSFORMASI GEN SOSUT1 PADA TANAMAN TEBU MENGGUNAKAN AGROBACTERIUM TUMEFACIENS STRAIN GV 3101 DAN PANGKAL TUNAS TEBU IN VITRO Edia, Edia F.D; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

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Gen SoSUT1 merupakan gen pengkode protein sucrose transporter yang memfasilitasi proses transpotasi sukrosa dari jaringan fotosintetik (source) ke jaringan pengguna (sink) pada tanaman tebu. Tujuan penelitian ini adalah mendapatkan tanaman tebu transforman melalui transformasi genetik menggunakan vektor Agrobacterium tumefaciens yang membawa gen SoSUT1. Eksplan pangkal tunas tebu in vitro diinfeksi dengan A. tumefaciens yang membawa konstruk pAct-SoSUT1 dan dilakukan seleksi pada media MS dengan penambahan antibiotik hygromycin.. Tanaman putatif transforman yang telah berhasil melewati proses seleksi dan diaklimatisasi, dilakukan isolasi DNA genom kemudian dianalisis PCR. Hasil analisis PCR dengan pasangan primer 1F/1R hpt II menunjukkan bahwa dari 24 tanaman tebu putatif transforman, didapatkan 15 tanaman tebu positif mengandung gen SoSUT1. Efektifitas rata-rata transformasi gen SoSUT1 menggunakan eksplan pangkal tunas tebu in vitro sebesar 6,8%. Kata Kunci: Agrobacterium tumefaciens, SoSUT1, sukrosa.

Partial Analysis of Probiotic Character of Lactic Acid Bacteria from the Intestines of Broilers Supplemented with Fish Protein Hydrolysate (FPH) Nashrullah, Shafa; Utami, Eva Tyas; Purniasari, Fina Yunita; Anggitasari, Dhanti Fatma; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53701

This study aims to analyze partial characteristics of probiotics lactic acid bacteria (LAB) from broiler intestines supplemented with fish protein hydrolysate (FPH) 2% (v/w) in feed. A total of 17 LAB isolates were successfully isolated and characterized morphologically and biochemically (catalase test). The isolate showed diverse morphological and biochemical properties, obtained as many as seven isolates that met the partial characteristics of probiotics, namely U10, U11, U13, U16, U31, U33, and U37 which were Gram-positive and no endospores and catalase formations were found. The tolerance test for acid pH and NaCl performed on the seven BAL isolates showed a diverse survival rate with U11 showing the highest tolerance at pH 2 (11.4%) and NaCl concentration up to 8% (32.4%). These findings highlight seven LAB isolates with partial probiotic properties, providing new insights into the development of probiotic candidates from broiler guts to improve gastrointestinal health and productivity.

Production and Characterization od Xylanase from Actinomyces ATG 70 Using CornCob Xylan Substrate Farrennina, Tasya Preira; Putri, Amelia Fahreza; Arimurti, Sattya; Winarsa, Rudju; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53702

Xilan is a polysaccharide that can be used as a substrate for producing xylanase. Xylan can be obtained from processing xylan-rich agricultural waste such as corn cobs, which contain 12.4% xylan. Xilan can be hydrolyzed into xylose and xylooligosaccharide (XOS) using the enzyme Xilanase. Actinomycetes have dominant xylanolytic species, Actinomyces ATG 70 has semi-qualitative xylanolytic ability with an enzyme activity index of 3.21±0.55 in xylan media. This study was conducted by extracting xylan from corn cobs, rejuvenating Actinomyces ATG 70, preparing the inoculum, creating a standard curve, producing xylanase with pH optimization of the medium, testing xylanase activity, characterizing the pH and temperature of crude xylanase, and identifying the morphology and biochemical characteristics of Actinomyces ATG 70. Optimization of the pH of the xylanase production medium was achieved at pH 8, with xylanase activity of 10.07±0.13 U/mL. The crude xylanase was characterized for pH and temperature effects, and the optimal pH for crude xylanase was found to be pH 6 with xylanase activity of 7.64±0.66 U/mL, and the optimal temperature was 50°C with xylanase activity of 11.17±0.33 U/mL. The identification results showed that Actinomyces ATG 70 belongs to Gram-positive bacteria, with a positive catalase test, white colonies, cream-colored aerial mycelium, and cream-colored pigmentation. The spore structure consists of long chains of conidia, streptococcus-shaped cells, and can form aerial mycelium, thus the isolate belongs to the genus Streptomyces.

The HEMOLYSIS ACTIVITY OF BACTERIA ISOLATS FROM PELANGI FOREST OF IJEN GEOPARK Arimurti, Sattya; Utarti, esti; sutoyo, sutoyo; siswanto, siswanto; Ayuningtyas, Tantri Raras
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44097

Ijen geopark is one of Indonesia's geoparks, which is located in East Java. A total of 153 bacteria have been isolated from Pelangi Forest, which were then given the isolate code IHP. These bacterial isolates can be utilized in industries, including organic matter decomposer agents, plant biocontrol agents, and probiotics. To ensure these bacteria are safe to use in various fields, they must be non-disease-causing (non-pathogenic). Safety evaluations based on the hemolysis reactions offer simple tests to ease the analysis of potential pathogenic bacteria. The study aimed to evaluate the safety of bacterial isolates from Pelangi Forest for their hemolysis reactions. The hemolysis test was conducted using blood agar media, from which isolates with a negative (Î») reaction. Based on the results of hemolysis tests, 30 out of 153 bacterial isolates (19.60%) were found to be negative reactions. These bacteria are safe to proceed with for potential analysis.

Lactic Acid Bacteria Isolated From Digestive Tract of Broilers Treated with Fish Protein Hydrolysate Utarti, Esti; Utami, Eva Tyas; Sjaifullah, Achmad; Handayani, Wuryanti; Belkis, Malika; Medayani, Rani Dian
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.38422

The composition of the feed plays a role in stimulating the activity of the microbiota in the gastrointestinal tract; therefore, the addition of fish protein hydrolysate (FPH) is suspected to influence the activity of microbiota, especially probiotics. Therefore, the presence of probiotics in the gastrointestinal tract affects the weight and quality of the broiler. This research aims to investigate the effect of administering fish protein hydrolysate as a dietary supplement on the composition of lactic acid bacteria (LAB), a potential probiotic candidate. This research was conducted in several stages, including the diversification of feeding broilers, the isolation and purification of LAB from the caecum and small intestine, primary characteristics, pathogenicity tests, and biochemical identification of LAB. Feed diversification was conducted by supplementing diets with 1%, 1.5%, and 2% FPH in 20-day-old for 7 days.. LAB from the small intestine and caecum samples were isolated on MRSA media by adding CaCO3. The Gram test, catalase test, and endospore staining test were carried out to characterize the suspected LAB primarily. The pathogenicity test was conducted by inoculating LAB on blood agar medium. Furthermore, biochemical tests are carried out using the KB020 kit. The results showed that the highest population of LAB in the small intestine (1.57 × 108 CFU/mL) was observed with 2% FPH supplementation. In comparison, the caecum yielded the highest population (1.22 × 108 CFU/mL) under 1.5% FPH. Giving 2% FPH to broiler chicken feed provides a weight gain of 1.021 kg/head. The primary characteristics of the eight bacterial colony isolates suspected of being LAB were Gram-positive, catalase-negative, and did not form endospores. Eight LAB isolates of probiotic candidates were non-pathogenic as indicated by the occurrence of α-hemolysis and γ-hemolysis. Biochemical identification of probiotic candidates yielded four types of Lactobacillus, namely L. mucosae, L. frumenti, L. sanfranciscensis, and L. ferintoshensis. These LAB strains show promising probiotic potential for use as a feed additive in the broiler production system.

Kapang amilolitik asal Biosite Hutan Pelangi, Ijen Geopark, Indonesia Sutoyo Sutoyo; Safira Isti’nafil Islam; Esti Utarti; Sattya Arimurti; Siswanto Siswanto
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p10

Vegetasi Hutan Pelangi, Ijen Geopark sudah terkonservasi sejak lama, sehingga kemungkinan di dalamnya terdapat diversitas mikrobiota yang potensial sebagai agen hayati pendegradasi berbagai substrat organik. Keberadaan dan pertumbuhan mikroba dalam lingkungan Hutan Pelangi berperan penting dalam membentuk proses antara lain siklus karbon, sehingga keberadaan mikroorganisme tersebut menarik untuk dikaji secara mikrobiologi. Penelitian ini bertujuan untuk mendapatkan isolat yang mampu mendegradasi substrat amilum secara semikuantitatif dan identifikasi secara morfologi sampai tingkat genus terhadap isolat terpilih. Penelitian diawali dengan isolasi, skrining kapang amilolitik serta identifikasinya secara fenotip terhadap isolat yang potensial aktivitas degradasinya tinggi. Hasil penelitian diperoleh 34 isolat kapang yang menunjukkan keragaman berbeda secara morfologi koloninya dan aktivitasnya dalam menghidrolisis amilum. Sejumlah 22 isolat (64,7%) menunjukkan aktivitas amilolitik dan 12 isolat (35,29%) merupakan kapang non-amilolitik. Hasil ANOVA rata-rata indeks aktivitas amilolitik isolat kapang dan uji lanjut Duncan menunjukkan bahwa isolat kapang IHP30, IHP10, dan IHP9 merupakan tiga isolat kapang amilolitik dengan indeks aktivitas amilolitik tertinggi. Indeks aktivitas masing-masing yaitu isolat kapang IHP30 sebesar 1,65 ± 0,14; IHP10 sebesar 1.47 ± 0,31; dan IHP9 sebesar 1.17 ± 0,06. Berdasarkan karakteristiknya, masing-masing teridentifikasi sebagai Penicillium sp. IHP30, Penicillium sp. IHP10, dan Aspergillus sp. IHP9

Kapang amilolitik asal Biosite Hutan Pelangi, Ijen Geopark, Indonesia Sutoyo Sutoyo; Safira Isti’nafil Islam; Esti Utarti; Sattya Arimurti; Siswanto Siswanto
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p10

Vegetasi Hutan Pelangi, Ijen Geopark sudah terkonservasi sejak lama, sehingga kemungkinan di dalamnya terdapat diversitas mikrobiota yang potensial sebagai agen hayati pendegradasi berbagai substrat organik. Keberadaan dan pertumbuhan mikroba dalam lingkungan Hutan Pelangi berperan penting dalam membentuk proses antara lain siklus karbon, sehingga keberadaan mikroorganisme tersebut menarik untuk dikaji secara mikrobiologi. Penelitian ini bertujuan untuk mendapatkan isolat yang mampu mendegradasi substrat amilum secara semikuantitatif dan identifikasi secara morfologi sampai tingkat genus terhadap isolat terpilih. Penelitian diawali dengan isolasi, skrining kapang amilolitik serta identifikasinya secara fenotip terhadap isolat yang potensial aktivitas degradasinya tinggi. Hasil penelitian diperoleh 34 isolat kapang yang menunjukkan keragaman berbeda secara morfologi koloninya dan aktivitasnya dalam menghidrolisis amilum. Sejumlah 22 isolat (64,7%) menunjukkan aktivitas amilolitik dan 12 isolat (35,29%) merupakan kapang non-amilolitik. Hasil ANOVA rata-rata indeks aktivitas amilolitik isolat kapang dan uji lanjut Duncan menunjukkan bahwa isolat kapang IHP30, IHP10, dan IHP9 merupakan tiga isolat kapang amilolitik dengan indeks aktivitas amilolitik tertinggi. Indeks aktivitas masing-masing yaitu isolat kapang IHP30 sebesar 1,65 ± 0,14; IHP10 sebesar 1.47 ± 0,31; dan IHP9 sebesar 1.17 ± 0,06. Berdasarkan karakteristiknya, masing-masing teridentifikasi sebagai Penicillium sp. IHP30, Penicillium sp. IHP10, dan Aspergillus sp. IHP9

Pemanfaatan Penanaman Cemara Udang sebagai Strategi Revitalisasi Kawasan Pantai dalam Rangka Optimalisasi Ekowisata Berkelanjutan Esti Utarti; Rendy Setiawan; Susantin Fajariyah; Mashuri Mashuri; Shafa Nashrullah; Tasya Preira Farrennina; Syafira Lailatul Ulfa Marfuah
JPM: Jurnal Pengabdian Masyarakat Vol. 6 No. 4 (2026): April 2026
Publisher : Forum Kerjasama Pendidikan Tinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jpm.v6i4.2592

Pancer Coastal is one of the southern sea beaches located in Puger Kulon Village, Jember. The characteristics of the beach and the sloping geographical allow Puger Kulon Village to develop into a beach-based tourism village. The southern sea has a harsh choppy character with sandy beaches so it needs plant vegetation that acts as a barrier. The need for vegetation as a barrier in the second zone besides the sea pandan which already exists as the first barrier initiated the planting of shrimp cypress in the Pancer coastal area. Planting shrimp cypress is beneficial in resisting wind speed and keeping air conditions cooler in addition to being able to reduce the impact of tsunamis and abrasion in coastal areas. Shrimp cypress can live in extreme conditions, resistant to drought and strong gusts of wind and pest attacks. This activity aims to revitalize the pancer beach area in order to support sustainable ecotourism. This activity is carried out through observation, coordination with related parties, provision and planting of shrimp cypress seedlings, shrimp cypress maintenance and provision of coastal environmental management modules. A total of 200 shrimp cypress seedlings have been planted on Pancer coastal involving the assisted village service team in collaboration with the village government, Pokdarwis and marine members from the Pam Puter XXVIII Nusa Barong Island Ops Task Force.

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