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All Journal Buletin Peternakan Jurnal Veteriner Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi Agrinimal Jurnal Ilmu Ternak dan Tanaman INDRA: Jurnal Pengabdian kepada Masyarakat Pakem : Jurnal Pengabdian Kepada Masyarakat Jurnal Teknologi Lingkungan Lahan Basah Jurnal Kedokteran Hewan
Rony Marsyal Kunda
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Jurnal Penelitian Pendidikan IPA (JPPIPA)

 1 
Study of Biomass in Two Mangrove Ecosystems Maman Rumanta; Sandra Sukmaning Aji; Rony Marsyal Kunda; Frenly Marvi Selano; Ucu Rahayu
Jurnal Penelitian Pendidikan IPA Vol 9 No 5 (2023): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i5.3755

Abstract

This study aims to determine the storage of biomass in the mangrove ecosystem of Jakarta Bay compared to Ambon Bay. This research used descriptive quantitative method. Sampling technique using the Point Center Quarter Method (PCQM) which was measured around the chest height of mangrove trees (DBH). Data analysis was performed using an allometric equation where each mangrove plant has a specific gravity. The results of this study indicated that the composition of mangrove species in Ambon Bay consists of 6 mangrove species, namely: Bruguiera cylindrica (L.) Bl, Rhizophora apiculata Bl, Sonneratia alba Smith, Aegiceras corniculatum (L.) Blanco, Avicennia officinalis L, Ceriops tagal. In the ecosystem, Rhizophora apiculata Bl and Sonneratia alba Smith had higher biomass values compared to the other four species. Meanwhile, in Jakarta Bay, the composition of mangrove plants is dominated by Sonneratia alba Smith. The subsurface biomass in the Jakarta Bay and Ambon region is lower than the aboveground biomass.
Animals in Tradition and Culture: An Ethnozoological Study of the Indigenous People of Seram Island Tjoa, Marthina; Kunda, Rony Marsyal; Lelloltery, Henderina; Puttileihalat, Maya MS
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.11286

Abstract

This study aims to examine the relationship between the indigenous people of Seram Island and several species of animals that are utilized for socio-cultural purposes and how the practice of ethnozoology can contribute to sustainable conservation efforts. The research was conducted using an ethnographic approach through in-depth interviews with purposively selected respondents. Qualitative descriptive analysis was conducted systematically based on themes that bind the types of animals studied. The results showed that the practice of ethnozoology includes utilization for ritual purposes related to local community belief values. Some animal species that are utilized for special purposes include Cuscuses (Phalanger spp.) and wild boar (Sus scrofa) which are useful for traditional ceremonies. The animals are killed and the meat is divided to be eaten during the ceremony. Gosong Bird (Eulipoa wallacei) as a Season Marker helps the community in managing agricultural land, marked by the presence or absence of these animals at certain locations. Deer (Rusa timorensis) as a Source of Food and Social Status by utilizing its meat. Cassowary (Casuarius spp.) as a symbol of strength. Hornbill (Rhyticeros plicatus) as a symbol of longevity. These animals are utilized by indigenous people not only for traditional rituals but also for animal protein needs such as cuscuses, Wild Boar and Deer. The needs of the indigenous community for the aforementioned animals have encouraged the community to maintain and preserve them based on their local wisdom such as the application of Sasi.
Polimorfisme Genetik dari Daerah 3'-Non Koding Region (3'-UTR) dari Gen HSP 70 pada Kerbau Moa (Bubalus bubalis) Rumanta, Maman; Hutasoit, Leonard Raden; Kunda, Rony Marsyal; Utami, Prasetyarti
Jurnal Penelitian Pendidikan IPA Vol 11 No 9 (2025): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i9.11685

Abstract

The Heat Shock Protein 70 (HSP70) gene, specifically the 3-Untranslated Region (3-UTR), is critical for regulating mRNA stability and translation under heat stress. This study aimed to identify genetic polymorphisms in the 3-UTR of the HSP70 gene in Moa buffalo (Bubalus bubalis) and evaluate its potential role in heat adaptation. A total of 65 hair follicle samples (55 Moa buffalo and 10 Banten buffalo) were collected, followed by amplification by PCR and sequencing of the PCR product of the 3'-UTR regions. Alignment results from the 3'-UTR region showed the presence of 2 major polymorphic SNPs, i.e g.1904C>A, and g.1910A>G. SNP (g.1904C>A) was found to be a cytosine (C) to adenine (A) substitution mutation type, and SNP g.1910A>G an adenine (A) to guanine (G) substitution. The discovery of 2 SNPs in the 3-UTR of the HSP70 gene in Moa buffalo is very important for the development of genetic markers, helping to understand the mechanism of heat adaptation, to the conservation and improvement of livestock performance, especially Moa buffalo living in tropical environments.
Impact of Environmental Geographic toward Point Mutations in Exon 1 of Growth Differentiation Factor (GDF9) Gene in Kosta and Lakor Goat Breeds Rumanta, Maman; Kunda, Rony Marsyal; Volkandari, Slamet Diah; Munir, Ivan Mambaul
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3794

Abstract

This study aims to analyze the impact of environmental biogeography on point mutations in Kosta and Lakor goat breeds as local Indonesian germplasm using the GDF9 Exon 1 gene sequence. A total 43 folicle samples of Kosta and Lakor goats which collected in Serang regency and Lakor island were analyzed. To isolate the total genome of the samples, the tissue isolation method was used. The polymerase chain reaction (PCR) method was used for amplification of the exon-1 region of the GDF9 gene with two pairs of primers. Analysis of genetic variation and single nucleotide polymorphisms (SNP) using MEGA software version X. Multiple alignment results showed that there are 2 nucleotides that have mutations, but not encode different amino acids. This results indicates that the mutation has association with litter size of two breeds. This finding shows that Lakor goats have polymorphism i.e SNP g.54C>T which is significantly associated with litter size. The analysis of GDF9 gene showed that all samples from the Two local Indonesian goat breeds (Kosta and Lakor) are  monomorphic. But, although both are monomorphic, geographical isolation still contributes to genetic drift within and between populations.
Biosensors Perancangan dan Pengembangan Biosensor untuk Deteksi Kualitas Perairan Laut Berbasis Piranti Mikrokontroler ESP32: Perancangan dan Pengembangan Biosensor untuk Deteksi Kualitas Perairan Laut Berbasis Piranti Mikrokontroler ESP32 Rumanta, Maman; Kunda, Rony Marsyal; Manuhutu, Fredrik
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.6944

Abstract

Water quality in cultivating marine organisms is a very important part to pay attention to. Prevention of pollution in marine waters needs to be done by recognizing changes in physical, chemical and biological parameters in these waters from normal conditions. To find out these changes, it is necessary to carry out measurements which include parameters that determine the quality of a liquid and carry them out periodically. The measurement parameters measured include pH, Dissolved Oxygen (DO), Electrical Conductivity (EC) and temperature. The use of technological developments, especially in the world of microcontrollers, namely the ESP32 device, makes it possible to design measurement instruments in determining the quality of sea waters to be more efficient and comprehensive. The instrument created in this research will be equipped with several sensors that will read the parameters that determine water quality. The sensors used are pH sensors, DO sensors, EC sensors and temperature sensors. This research aims to create an integrated instrument (pH, DO, ED and Temperature) with instrument performance specifications that have good accuracy and a portable design so that it is easy to use in the field. The research results show thatdevelopment of a biosensor instrument for measuring seawater quality based on the ESP32 DevKit V1 can work well and can display temperature, degree of acidity (pH), DO and EC parameters on the LCD screen according to actual conditions with a difference range in percent between 0.2% - 4.16 %. The accuracy level of the designed tool is classified as having a high accuracy above 95%
Bioaccumulation of Pb in the Gills of Perna viridis (Bivalvia: Mytilidae) in Jakarta Bay, Indonesia and its Histological Alteration Kunda, Rony Marsyal; Rumanta, Maman; Hutasoit, Leonard Raden
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.7109

Abstract

Pollution in Jakarta Bay associated with terrestrial run-off and eutrophication. Bivalves have been recognized as aquatic organisms that play the most dominant role in aquatic ecosystems. Pb is an element that in many cases causes health problems in the liver, kidneys, hematopoietic system and nervous system. This study aims to analyze the bioaccumulation of Pb in the gills of P. viridis in Jakarta Bay, and its histological changes. Bioaccumulation analysis using the AAS and histology changes using transmission electron microscopy (TEM) methods. The results of AAS, showed that the value of Pb concentration in gill tissue at the two seasons did not show a noticeable difference. Pb concentrations in gills were lower at 1, 4, and 5 than at 2 and 3 stations. The results of ultrastructure analysis as an impact of Pb accumulation showed that thickening of the nucleus membrane, and a decrease in the number of microvilli. The thickening of the nucleus membrane, and the decrease in the number of microvilli are cellular responses to the entry of the Pb heavy metal into the cell. Besides, the results of this study showed inflammation, a decrease in the number of mitochondria, as well as causing cristae to be empty.
Design and Construction of a Real-Time Air Quality Monitoring System Using IoT-Based ESP32 to Strengthen Environmental Policies El Anshori , Yuli Tirtariandi; Kunda, Rony Marsyal; Manuhutu, Fredrik
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.9820

Abstract

Air quality monitoring is one of the important steps in maintaining public health and the environment. With the development of Internet of Things (IoT) technology, air quality monitoring can be done in real-time and more efficiently. This study aims to environmental policy and design of an IoT-based air quality monitoring system using the ESP32 microcontroller. This system is designed to measure air quality parameters such as CO, NO2, temperature, and humidity using factory-calibrated sensors (DFRobot) connected to the ESP32 microcontroller. Data obtained from the sensors are processed by the ESP32 and sent to a cloud server via Wi-Fi, allowing real-time monitoring via the ThingSpeak platform which can be monitored via mobile devices or the web. The results of the air quality monitoring system design show that devices using electrochemical CO and NO sensors₂and the SHT30 sensor connected to the ESP32 is capable of reading and measuring CO, NOconcentrations., temperature, and humidity with good accuracy with a sample time of ± 20 seconds. In addition, this system can be connected online with the ThingSpeak platform, allowing visualization of measurement data in graphical form in real-time. Thus, the designed system not only functions optimally in detecting air quality parameters, but also supports efficient remote monitoring through Internet of Things (IoT) technology
EKSPLORASI METAGENOMIK DARI SERANGGA BLACK SOLDIER FLY (Hermetia illucens) PENDEGRADASI SAMPAH ORGANIK DALAM UPAYA BIOREMEDIASI LINGKUNGAN irwanto; Rumanta, Maman; Kunda, Rony Marsyal
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9926

Abstract

The Black Soldier Fly (BSF) (Hermetia illucens Linnaeus, 1758) has long been recognized as an organism used in organic waste processing through bioconversion methods. H. illucens is known to digest organic materials into nutrient sources utilized for biomass formation with the assistance of decomposer microbiota. However, research on the structure and composition of its microbiota remains limited. This study aims to identify microbiota and their structural composition in both the larval and adult fly phases, based on organic waste feeding in tropical regions. Additionally, it seeks to provide recommendations for relevant stakeholders in identifying potential environmental bioremediation agents. The research method employed is a survey study with quantitative sample analysis. The amplification process in this study uses primers from the (V1-V9) regions of the 16S rRNA gene. Data analysis is conducted using the QIIME (Quantitative Insights into Microbial Ecology) method, utilizing high-throughput sequencing community data with QIIME2 software version 3.5.3. Microbiota from the families Lactobacillaceae and Morganellaceae have been identified as dominant in larvae, while Staphylococcaceae and Bacillaceae dominate in adult flies. Morganella morganii, Herbaspirillum piri, Dysgonomonas capnocytophagoides, and Clostridium intestinale are potential candidates for organic waste bioremediation from BSF larvae. Meanwhile, Sphingobacterium wenxiniae, Lachnoclostridium phytofermentans, Mammaliicoccus sciuri, and Corticicoccus populi are bioremediation candidates from BSF flies. The genera Enterococcus, Morganella, and Dysgonomonas are found in both temperate and tropical climate regions. However, Providencia, Klebsiella, Scrofimicrobium, and Actinomyces, which are found in the gut of BSF larvae in temperate regions, are absent in BSF larvae from tropical Indonesia. Conversely, Limosilactobacillus, Entomomonas, Lachnoclostridium, and Clostridium are not found in the gut of BSF larvae in temperate regions.
Co-Authors El Anshori , Yuli Tirtariandi Fredrik Manuhutu, Fredrik Habibi Habibi Hendrina Lelloltery Hery Wijayanto Heryanus Jesajas Hutasoit, Leonard Raden IRWANTO Jamhari Jamhari Lakoan, Milda Rianty Lokollo, Richard R. Lokollo, Richard Rudolf Maman Rumanta Marleny Leasa Moniharapon, Mechiavel Munir, Ivan Mambaul Niken Satuti Nur Handayani Niken Satuti Nur Handayani Pieter Kakisina Porumau, Yandri Yanes Prasetyarti Utami Puttileihalat, Maya MS Rini Widayanti Salamor, Reinhard Sandra Sukmaning Aji Selanno, Frenly Marvi Slamet Diah Volkandari Slamet Diah Volkandari, Slamet Diah Sri Kurniati Handayani Tjoa, Marthina Ucu Rahayu Utami, Prasetyarti Woro Danur Wendo Yuli Tirtariandi El Anshori