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Woro Danur Wendo
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Histological Features of Catecholaminergic Neuron in Substantia Nigra Induced by Paraquat Dichloride (1,1-dimethyl-4,4 bipyridinium) in Wistar Rat as A Model of Parkinson Disease Pangestiningsih, Tri Wahyu; Wendo, Woro Danur; Selan, Yulfia Nelymalik; Amalo, Filphin Adolfin; Ndaong, Nemay Anggadewi; Lenda, Victor
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.756 KB)

Abstract

Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquatdichloride is harmful if enters to the body, causing Parkinson’s disease, since it is disrupting dopamineproduction in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The studywas done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway causedby paraquat dichloride treatment in Wistar rats as a model of Parkinson’s disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group)were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquatdichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected onceper week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg)and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline asprerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brainswere removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry stainingusing tyrosine hydroxylase antibody. The results of staining then were observed under light microscope andanalyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigrapars compacta in all treatment groups, however, the cell density were found decreased only in group IV.Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine ‘Nigro striatal pathway’was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergicneurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.
Histological Features of Catecholaminergic Neuron in Substantia Nigra Induced by Paraquat Dichloride (1,1-dimethyl-4,4 bipyridinium) in Wistar Rat as A Model of Parkinson Disease Tri Wahyu Pangestiningsih; Woro Danur Wendo; Yulfia Nelymalik Selan; Filphin Adolfin Amalo; Nemay Anggadewi Ndaong; Victor Lenda
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.756 KB) | DOI: 10.22146/ijbiotech.8638

Abstract

Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquatdichloride is harmful if enters to the body, causing Parkinson’s disease, since it is disrupting dopamineproduction in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The studywas done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway causedby paraquat dichloride treatment in Wistar rats as a model of Parkinson’s disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group)were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquatdichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected onceper week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg)and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline asprerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brainswere removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry stainingusing tyrosine hydroxylase antibody. The results of staining then were observed under light microscope andanalyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigrapars compacta in all treatment groups, however, the cell density were found decreased only in group IV.Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine ‘Nigro striatal pathway’was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergicneurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.
MANFAAT RUMPUT LAUT (Eucheuma spinosum) UNTO( MENINGKATKAN KUALITAS SPERMA TIKUS AKIBAT PEMBERIAN TIMAH HTTAM = THE USEFUL OF SEAWEED (Eucheuma spinosum) TO ACCELERATE THE SPERM QUALITY OF RATS TREATED WITH LEAD Bambang Dwi Haryanto; Woro Danur Wendo; Hendri T.S. Saragih; Arif Suhodo; Yuliati Wahyu S.; Siti Isrina Oktavia S.
Jurnal Sain Veteriner Vol 19, No 2 (2001): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.378

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian rumput laut terhadap kualitas sperma tikes akibat pencemaran timah hitam. Dalam penelitian ini digunakan 12 ekor tikus putib jantan dewasa, dibagi secara acak menjadi 4 kelompok masing-masing 3 ekor. Kelompok I, tikus-tikus hanya diberi akuades secara oral. Kelompok11,111 dan IV, semua tikus diberi Pb asetat 0,5 g/kg bb secara oral setiap hart selama 120 hart. Kelompok DI dan IV masing-masing disertai dengan pemberian larutan rumput laut dosis 8 g/kg dan 16 g/kg bb secara oral pe, hari selama 120 hart_ Pada akhir penelitian masing-masing films dart semua kelompok dikoleksi spermanya untuk dilakukan pemeriksaan terhadap motilitas, viabilitas dan konsentrasi apermanya. Semua tikus percobaan kemudian dietanasi dan dinekropsi, organ testis diambil dan difiksasi dalam ICJ% formalin untuk preparasi histopatologis. Hasil penelitian memperlihatkan adanya peningkatan motiiitas, kemampuan hidup dan konsentrasi spermatozoa secara sangat signifikan (P
Identifikasi Keragaman Genetik Gen 12S Ribomsom RNA Sebagai Penanda Genetik untuk Penentuan Spesies Kuskus (IDENTIFICATION OF GENETIC DIVERSITY 12SRRNA GENES AS GENETIC MARKER FOR DETERMINING SPECIES CUSCUS) Rini Widayanti; Hery Wijayanto; Woro Danur Wendo; Rony Marsyal Kunda
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cuscus is marsupial’s animal (Phalageridae) which has limited spread in eastern Indonesia (Sulawesi,Maluku, Papua), Australia and Papua New Guinea. The ex-situ and in-situ conservation of cuscus undercaptivating condition is an alternative solution to protect cuscus from extinction. This study aimed todetermine nucleotide sequence and genetic markers on 12Sr RNA gene with sequencing method of eachspecies on three islands. Whole genome DNA was extracted from 17 samples of cuscus obtained fromdifferent habitats, Sulawesi (2 individual), Maluku (7 individual), and Papua (8 individual) according tothe protocol of Qiamp DNA Blood Mini Kit (Qiagen), and then it was used as template for amplificationof 12Sr RNA gene by using PCR. PCR product were then purified using column chromatography and wereused as template for sequencing reaction. Result sequencing of 12Sr RNA gene were analyzed usingMEGA program version 6. PCR product gives a result nucleotida of 958 bp according to databasegenebank, sequencing product gives result nucleotida of 896 bp and found of 105 different nucleotide sites.Filogram based on nucleotide sequences 12SrRNA gene from Sulawesi cuscus is Ailurops ursinus whereasthe cuscus from Papua and Maluku is Phalanger sp. and Spilocuscus maculatus species. Thirteen nucleotidasites were found, sites no 67 (A/G), 89 (G/C), 137 (T/C), 285 (G/A), 468 (T/C), 595 (T/C, 598 (T/C), 647 (T/C),654 (G/A), 665 (T/C), 769 (C/T), 874 (C/T), and 876 (A/G) which can be used as genetic marker betweenPhalanger genera from Papua and Maluku, and three nucleotida sites (sites no 127 (G/A), 481 (C/T), and885 (T/C) can be used as genetic marker between Spilocuscus genera from Papua and Maluku.
Lumba-Lumba Hidung Botol Laut Jawa Adalah Tursiops aduncus Berdasar Sekuen Gen NADH Dehidrogenase Subunit 6 (VERIFICATION BOTTLENOSE DOLPHINS FROM JAVA SEA IS TURSIOPS ADUNCUS BASED ON GENE SEQUENCES OF NADH DEHYDROGENASE SUBUNIT 6) Rini Widayanti; Yuda Heru Fibrianto; Woro Danur Wendo
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bottlenose dolphins (Tursiops sp.) is one of the aquatic mammals widely spread in the marines ofIndonesia archipelago, especially the Java Sea. The taxonomy of the genus Tursiops is still  controversial.The purpose of this study was to examine the molecular basis of Tursiops sp of Java sea marine origin onthe basis of its NADH dehydrogenase gene subunit 6 (ND6) sequences. Samples of blood were collectedfrom five male bottle nose dolphins from captivity of PT. Wersut Seguni Indonesia. DNA was isolated,amplified by polymerase chain reaction (PCR), sequenced, and analyzed the data using the MEGA v. 5.1program. The results of PCR amplification was 868 base pairs (bp), DNA sequencing showed that 528nucleotides were ND6 gene, nucleotide at the position of 387 could be used to distinguish the bottle nosedolphins Java marine origin with T. aduncus.   Filogram using Neighbor joining method based on thenucleotide sequence of the gene ND6, showed that bottle nose dolphins Java marine origin belong to groupof T. aduncus.
Development of Skeletal Ossification in Climbing Perch (Anabas testudineus) from Juvenile to Adulthood Pangestiningsih, Tri Wahyu; Wendo, Woro Danur; Kusindarta, Dwi Liliek
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.310-319

Abstract

The climbing perch (Anabas testudineus) is a freshwater fish that can withstand highly unfavorable environments and stay out of the water for extended periods. Its anatomical characteristics showed terrestrial adaptation and terrestrial locomotion properties enable the use of climbing perch as an animal model. Moreover, its bone and cartilage profiles at different stages are crucial to improving the anatomical information for the osteogenesis model candidate. The current study aimed to illustrate the skeletal profiles of juvenile and adult climbing perch with the whole staining method. The samples included two adults and two juvenile climbing perch from Daerah Istimewa Yogyakarta, Indonesia. The fish were euthanized using β-hydroxyethyl phenyl ether in a lethal dose. The skeleton was examined using the Alcian blue–Alizarin red whole staining, which revealed the bones and cartilage under the stereomicroscope. The result showed that the bone is the main skeleton. Cartilage was detected in the area surrounding the orbit in the adult climbing perch and in the pterotic, pterosphenoid, prootic, and distal end of the hypural, parhypural, and basal pectoral girdle in the juvenile climbing perch. Endochondral osteogenesis was found in juvenile to adult climbing perch.
Co-Authors Arif Suhodo Bambang Dwi Haryanto Dwi Liliek Kusindarta Filphin Adolfin Amalo Hendri T.S. Saragih Heni Pujiastuti Hery Wijayanto Nemay Anggadewi Ndaong Nemay Anggadewi Ndaong, Nemay Anggadewi Rini Widayanti Rony Marsyal Kunda Siti Isrina Oktavia S. Tri Wahyu Pangestiningsih Victor Lenda Yuda Heru Fibrianto Yulfia Nelymalik Selan Yulfia Nelymalik Selan, Yulfia Nelymalik Yuliati Wahyu S.