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Journal : The Journal of Pure and Applied Chemistry Research

Analysis and Prediction of some Histone-derived Antimicrobial Peptides from Toads Duttaphrynus melanostictus and Phyrinoidis asper Muhammad Dailami; I Made Artika; Mirza Dikari Kusrini
The Journal of Pure and Applied Chemistry Research Vol 5, No 2 (2016)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1579.558 KB) | DOI: 10.21776/ub.jpacr.2016.005.02.231

Abstract

Antimicrobial peptides in skin secretions of toads is a promising methods to combat a wide spectrum of bacteria. Histone H2A is a type of DNA-binding protein that acts as a precursor for several antimicrobial peptides. In toads (family Bufonidae) buforin I and buforin II are examples of antimicrobial peptides that derived from histone H2A. This study investigated the genetic diversity and phylogenetic analysis and in silico prediction of antimicrobial peptides derived from histone H2A of Duttaphrynus melanostictus and Phyrinoidis asper, which were collected from Bogor Agricultural University’s campus area. A new set of primers (Buf_fwd and Buf_rev) were designed by using PrimerBLAST, to amplify 393 nucleotides of the histone H2A gene that codes 131 amino acids. Haplotype diversity of both species are very low. Phylogenetic analysis shows the sample D. melanostictus and P. asper are separated to each other in two different clades. Several short predicted peptides from histone H2A show a potential as an antimicrobial peptides based on in silico prediction. Psychochemical characteristics and 3D structure of potent antimicrobial peptides are described.
Co-Authors Adininggar Ul-Hasanah Agus Priyono Kartono Aini, Yusratul Andayani, Noviar Andhika Prima Yudha Ani Mardiasturi Ani Mardiastuti Anika Putri Aprilianti, Risma Aria Nusantara Arief Tajalli Ariefiandy, Achmad Arzyana Sunkar Bakhtiar Aji Boby Darmawan Burhanuddin Masy'ud Burhanuddin Masy'ud Burhanuddin Masy'ud Burhanuddin Masy’ud Darmawan, Boby DEDE AULIA RAHMAN Dian Oktaviani Dodi Safari Duto Nugroho Dwi Fania Endang Hernawan Erna Suzanna Eterna Firliansyah Evy Arida Fata Habiburrahman Faz Fata Habiburrahman Faz Fauzia, Alienda M Frayoga, Yunus Arif Fredinan Yulianda Guntoro, Joko Hadi S Alikodra Hadi S. Alikodra Hanny Herzegovina Harnum Nurazizah I MADE ARTIKA Ida Ayu Ari Janiawati Ira Khoerunisa Jakaria, Mohamad Kaniwa Berliani Kaniwa Berliani Kaniwa Berliani Kennedi, Umar Fhadli Kristina Nainggolan Larasati, Firda LILIK BUDIPRASETYO Loar, Loise Liberta Luna Raftika Khairunnisa Luthfia Nuraini Rahman Maddock, Simon T Mahmud, Arief Mohamad Jakaria Mohammad Ali Ridha Mohammad Mukhlis Kamal Muhamad Kurniawan, Muhamad Muhammad Dailami, Muhammad MUHAMMAD IRFANSYAH LUBIS Muhammad Irfansyah Lubis Nancy Karraker Noor Farikhah Haneda Novi Tri Ayuningrum Nusantara, Muhammad Galih Gurenda Prabowo, Oktavianus Jekki Pranata, I Putu Agus Widi Purwantono Purwantono Rahman, Luthfia Nuraini Rahmat Abdiansyah Ramadhan, Tubagus Muhamad Daffa Ramdani Manurung Ramdani Manurung Resti Meilani Rodrigo Ibrrandi Vazquez Sambas Basuni Sari, Fitri Eka Sawaki, Morgan Wayne Luther Setiawan, Rezky Agust Sigit Purwanto Suci Ninda Utari Tiara, Alma Vidya Elza Loriza Wempy Endarwin Yanto, A Viktor Yeni Aryati Mulyani Yudi Setiawan Zainudin