Article Per Year (5 Year)
p-Index From 2021 - 2026
2.064
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Bios Logos Jurnal Pengajaran MIPA Jurnal Pengajaran Matematika dan Ilmu Pengetahuan Alam Formica Online Jurnal Matematika & Sains Jurnal Pendidikan Biologi Indonesia Assimilation: Indonesian Journal of Biology Education Lectura : Jurnal Pendidikan Jambura Edu Biosfer Journal Aquacultura Indonesiana Indobiosains Asean Journal of Science and Engineering (AJSE) Gunung Djati Conference Series Sasambo: Jurnal Abdimas (Journal of Community Service)
Diah - Kusumawaty
Universitas Pendidikan Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Other

Published : 17 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : HAYATI Journal of Biosciences

 1 
Effectivity of Silver Nanoparticles-Temu Giring (Curcuma heyneana) Rhizome on Inhibiting the Growth of Bacteria Causing Nosocomial Infection Nurjamil, Aris Muhamad; Ekajaya, Renandy Kristianlie; Saputro, William Junino; Sururi, Zaki Fahreza; Kusumawaty, Diah
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.284-292

Abstract

Biofilms are a common cause of nosocomial infections that often attack hospitalized patients. The main objective of this study was to examine the efficacy of silver nanoparticles-temu giring rhizomes in combating bacteria and preventing biofilm formation. The antibacterial and antibiofilm properties of these nanoparticles were evaluated against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The research began with the extraction of temu giring rhizome, synthesis of silver nanoparticles-temu giring rhizome, disk diffusion test, biofilm formation inhibitory activity test, and characterization of silver nanoparticles-temu giring rhizome. In this research, silver nanoparticles-temu giring rhizome were utilized at concentrations of 10, 20, 40, 80, 160 μg/ml, and a control in the form of chlorhexidine. The results showed that the silver nanoparticles-temu giring rhizome produced a larger inhibition zone for bacterial growth compared to the control against the three bacteria. The IC50 value of silver nanoparticles-temu giring rhizome required to inhibit biofilm formation was 27.64 μg/ml in E. coli, 29.29 μg/ml in P. aeruginosa, and 26.21 μg/ml in S. aureus. In P. aeruginosa, E. coli, and S. aureus, the IC50 for preventing biofilm formation by silver nanoparticles-temu giring rhizome was determined to be 27.64 μg/ml, 29.29 μg/ml, and 26.21 μg/ml, respectively. Evaluation of silver nanoparticles revealed the success of temu giring rhizomes in reducing silver ions. This is shown that silver nanoparticles-temu giring rhizomes can be developed into active ingredients that inhibit the growth of bacteria that cause nosocomial infections.
Successful Primer Picking and Pooling for the Design of Multiplex PCR Primers Specific to Pork, Beef, Chicken, and Rat DNA Kusumawaty, Diah; Faridah, Nurul; Fibriani, Azzania; Priyandoko, Didik; Dzikrina, Hanina; Puspitasari, Diah; Tallei, Trina Ekawati; Aryani, Any
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.678-686

Abstract

DNA markers and Multiplex-PCR have emerged as methods for species detection in processed meat products. The primary objective of this study is to design multiplex primer sequences for pork, rat, beef, and chicken, generating distinguishable amplicons through agarose gel electrophoresis for halal detection in processed meat products. Primer design involved utilizing mitochondrial genomic data and the NCBI-Primer BLAST site to obtain specific pork and beef primer sequences. In silico simulations, including single and multiplex-PCR, were conducted using Primer Pooler. In vitro validation encompassed Single-PCR and Multiplex-PCR annealing temperature optimization, using samples of chicken, beef, pork, and rat as well as processed meat products like meatballs, sausages, and nuggets. In vitro validation demonstrated that the halal marker gene's multiplex primer efficiently amplified the target sequence, specifically at the optimal annealing temperature of 58°C. Amplicons from beef (1,217 bp), pork (860 bp), rat (622 bp), and chicken (272 bp) primers could be distinguished on a 1.5% agarose gel. The study's results can aid in cost-effective and rapid halal testing and authentication of processed meat products, offering advantages over PCR with a single primer.
Co-Authors Abd. Rasyid Syamsuri Afifi Mutiarani Any Aryani Astry Agusthina Muchtar Azzahra, Waliyyatu Azzania Fibriani Dennisa Ameria Sendjaya Diah Puspita Sari Diah Puspita Sari Diah Puspitasari Dian Din Yati Diana Rochintaniawati Diana Rochintaniawaty Didik Priyandoko Dina Mariana Dzikrina, Hanina Ekajaya, Renandy Kristianlie Eliya Mei Sisri Fadillah, Nuke Siti Geovanni Pratama Putri Gusni Nugraha As-Syiba Hanif Syafrian Purnama Hanina Dzikrina Irna Riski Kardila Jayah, Johan Susanto Kartika Khadafiah Mutia Wiandari Khansa Biantari Alika Kusdianti Kusdianti Kusdianti Kusdianti Kusumawardhani, Meirina Kartika Lulu Desia Mutiani Rahmayuni Mr Kusnadi Nurjamil, Aris Muhamad Nurul faridah Nurul Faridah, Nurul Nuryani Rustaman Salma Annisa Nur Alifah Salsabila Shafa Saidah Saputro, William Junino Shafira Lestari Shintya Yuniar Siti Awalia Nur Fadillah Siti Sriyati SONY SUHANDONO Suroso Adi Yudianto Surtikanti Hertien Koosbandiah, Surtikanti Sururi, Zaki Fahreza Topik Hidayat TRINA EKAWATI TALLEI Try Kurniawan Visi Tinta Manik Yayan Sanjaya