Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

P-Index

This Author published in this journals

All Journal Microbiology Indonesia Indonesian Journal of Cancer Chemoprevention Majalah Obat Tradisional

Subagus Wahyuono

Faculty of Pharmacy, Universitas Gadjah Mada

Author-ID : 3038034

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Published : 14 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

Macroscopy and Microscopy Study of Scopraia dulcis L. Djoko Santosa; Subagus Wahyuono; Sugeng Riyanto; SM Widyastuti
Majalah Obat Tradisional Vol 23, No 1 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

A morphological diversity analysis of Scoparia dulcis by macroscopy and microscopy has been conducted. The aim of this research is to determine the morphological and anatomical character of S. dulcis which grow in Opak watershed, Special Region of Yogyakarta. Samples were taken from Sleman (Cangkringan, Ngemplak Kalasan, and Prambanan) and Bantul (Imogiri and Pundong) region. Selection of sampling location is based on altitude difference. The samples are the third leaf order from the tip of the stem. They were measured in length and width. Microscopy analysis was performed by observing the number of glandular trichomes, palisade cells in the epidermis and stomata in the upper and lower epidermis. The data obtained were analyzed using principal component analysis (PCA). The results show that based on morphological and anatomical assessment, there were found 3 clusters of S. dulcis, i.e. Cangkringan-Ngemplak-Prambanan, Kalasan, and Imogiri-Pundong cluster.

Topical Anti-inflammatory Activity of Bangle (Zingiber cassumunar Roxb). Ethanolic Extract in Mice Induced By Carrageenan Endang Dwi Wulansari; Subagus Wahyuono; Marchaban Marchaban; Sitarina Widyarini
Majalah Obat Tradisional Vol 23, No 2 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Rhizome of bangle (Zingiber cassumunar Roxb.) is one of the materials that are traditionally used in the traditional preparation to maintain skin from redness. The content of chemical compound in bangle rhizomes has many benefits, including inhibition of the inflammatory processes. This study aims to determine the topical anti-inflammatory activity of ethanolic extract of bangle rhizomes in mice induced by carrageenan. Thirty five female Swiss mice were used in this study. Animals were divided into 7 group of five: normal group, carrageenan 2% group, carrageenan 2% group plus control of basis (Biocream®), carrageenan 2% group plus 2.5% hydrocortisone cream, and carrageenan 2% group plus cream of ethanolic extract of bangle rhizomes with the concentration of 1%, 2.5% and 5% w/w. Carrageenan 2% was injected subcutaneously in all treatment group except normal group. The skinfold thickness of mice was measured every 1 hour for 6 hours after carrageenan injection. Results of this study shows that there is a significant differences between treatment groups (p <0.05) compared to carrageenan 2% treated group. To conclude, ethanolic extract of bangle rhizomes has topical anti-inflammatory in mice induced by carrageenan.

Isolation and Identification of Emestrin from Emericella nidulans and Investigation of Its Anticancer Properties MUHAMMAD NURSID; EKOWATI CHASANAH; . MURWANTOKO; SUBAGUS WAHYUONO
Microbiology Indonesia Vol. 5 No. 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

The research to isolate, identify and investigate of anticancer properties of active compound produced by Emericella nidulans marine fungus has been done. Active compound was isolated from mycelium extract of the fungus. The molecular formula of active compound was established as C27H21N2O10S2 by LC-ESI-ToF-MS (m/z 597.1105 [M – H]-. Elucidation of molecular structure using FT-IR, LC-ESI-ToF-MS, 1H-NMR, 13C-NMR, and DEPT 135˚ showed that the active compound was emestrin. Emestrin was found to have cytotoxic effect against T47D, HepG2, C28, and HeLa but it was not too toxic against Vero cells with IC50 value of 1.8 µg mL-1, 4.2 µg mL-1, 2.6 µg mL-1, 13.8 µg mL-1, and 260.9 μg mL-1, respectively. Base on the cell cycle analysis by using flow cytometry, emestrin treatment at concentration of 1.0 μg mL-1 induced cell-cycle arrest in G0/G1 phase whereas at concentration of 3.0 μg mL-1, a sub-population of cells (sub G1) appeared. The apoptosis assay by using Annexin-V-FLUOS revealed that most of T47D cell treated with the compound at 1.0 and 3.0 μg mL-1 underwent apoptosis (83.6% and 92.6%, respectively. This anticancer activity of emestrin may be related to the unique of the epithiodioxopiperazine moiety with internal disulphide bond of this compound.

SPF Test from Baccaurea lanceolata Muell.Arg Fruit Isolates Samsul Hadi; Subagus Wahyuono; Ag. Yuswanto; Endang Lukitaningsih
Indonesian Journal of Cancer Chemoprevention Vol 8, No 1 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss1pp38-41

Not many researches on a fruit of limpasu (Baccaurea lanceolata Muell. Arg ) were found, while the use by tribal hamlets in Kalimantan already became a daily routine before they went into the field with the way made powder and smeared on the face. Therefore, the research on active substances needs to be done. For this study, we prepared two isolates which coded as K2 and CE, K2 from qualitative test negative isolates phenolic groups was identified by spray FeCl3, whereas qualitative test positive isolates were coded as CE and detected by a nitrogen-containing reagents dragendorf spray. The K2 isolate from SPF test resulted an SPF value of 3.2 at a concentration of 0.2 mg/ml, while CE isolate was given a value of SPF 5.0 at a concentration of 0.2 mg/ml. According to sensitivity skin test on rabbit skin, both K2 and CE isolated were given the value below 1, indicated the isolates were found to not irritate. This study proposes the potential from Baccaurea lanceolata Muell.Arg fruit isolates to be developed as a sunscreen for UV-protection.Keywords : Baccaurea lanceolata Muell.Arg, SPF test, sensitivity skin test

Co-Authors Ag. Yuswanto Ari Sartinah Djoko Santosa EKOWATI CHASANAH Endang Dwi Wulansari Endang Lukitaningsih Erna Prawita Setyowati Isnindar Isnindar Marchaban Marchaban Mimiek Murrukmihadi Mufrod Mufrod MUHAMMAD NURSID Murwantoko . Nanang Fakhrudin, Nanang Peni Susilowati Putri Puji Astuti Samsul Hadi Sitarina Widyarini Sitarina Widyarini SM Widyastuti Sri Sudewi, Sri Sri Wahdaningsih Sudibyo Martono Sugeng Riyanto Sutomo Sutomo Suwaldi Suwaldi Yuswanto Yuswanto

Title

Found 14 Documents
Search

Abstract

Abstract

Abstract

Abstract

Title Search

Found 14 Documents Search

Abstract

Abstract

Abstract

Abstract

Title

Found 14 Documents
Search