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Journal : AL KAUNIYAH

Aktivitas Ligninolitik Beberapa Jamur Aphyllophorales dan Kemampuannya Mendegradasi Lignin pada Lindi Hitam Atria Martina; Tetty Marta Linda; Delita Zul; Nila Veronika; Ratna Jelita
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v8i1.2702

Abstract

Fourteen local isolate Aphyllophorales fungi were screened their ligninolytic activity. The isolate with highest ligninolytic activity was tested it capability to degrade kraft blackliquor lignin. The biodegradability of black liquor is low because the presence of lignin and lignin derivative in the wastewater. These fungal were screened for ligninolytic activity by decolorization on solid mediacontaining RBBR dye. The ability of the fungal strains to biodegrade kraft black liquor lignin was performed by submerged fermentation condition with agitation and incubation time as treatment. The solid culture result in 3 isolates had ligninolytic activity and Ganoderma sp.BTA1 gave the highest ligninolytic. Agitation and incubation time influenced ligninbiodegradation of blackliquor significantly. Optimum condition for lignin biodegradation was at 200 rpm during 25 days with lignin reduction was 45,786%.
ISOLASI DAN KERAGAMAN BAKTERI UREOLITIK LOKAL RIAU YANG BERPOTENSI SEBAGAI CAMPURAN BETON Mufidah Dwi Suci Ningsih; Tetty Marta Linda; Bernadeta Leni Fibriarti
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (619.771 KB) | DOI: 10.15408/kauniyah.v11i1.5737

Abstract

Abstrak Bakteri ureolitik merupakan mikroorganisme yang memiliki kemampuan untuk menghasilkan urease yang dapat mengendapkan kalsium karbonat (kalsit). Bakteri ini berpotensi sebagai agen bioremediasi logam berat dan sebagai bahan konstruksi beton. Penelitian ini bertujuan untuk mengisolasi, mengkarakterisasi, dan menguji presipitasi kalsit bakteri ureolitik dari tanah lokal Riau. Hasil penelitian memperoleh 30 isolat bakteri yang berhasil diisolasi dari tanah tempat pembuangan akhir, dan setelah dilakukan pewarnaan Gram, 77% isolat merupakan bakteri Gram positif dan hanya 33% merupakan bakteri Gram negatif.  Hasil uji presipitasi menunjukkan bahwa isolat- isolat dengan kode sp. 32, sp. 9, dan sp. 20 mampu membentuk kalsium karbonat berturut-turut sebesar 0,129 g, 0,126 g, dan 0,105 g, setelah diinkubasi selama 7 hari pada medium cair yang diberi penambahan urea dan kalsium. Isolat-isolat bakteri tersebut memiliki hubungan kekerabatan yang dekat, yang ditandai dengan besarnya koefisien kekerabatannya, yaitu lebih dari 70%. Dengan demikian, bakteri-bakteri yang terisolasi dan teruji dalam  membentuk kalsium karbonat asal tanah lokal Riau berasal dari sekelompok bakteri, yang berpotensi untuk dikembangkan sebagai campuran beton.  Abstract Ureolytic bacteria are microorganisms that have the ability to produce urease that precipitates calcium carbonate (calcite). This bacteria has potential as an agent for bioremediation of heavy metal and as a concrete construction material. The aim of this research is concerning about isolation, characterization, and examination on calcite precipitate of the ureolytic bacteria from Riau local soil. The result showed that 30 isolates were isolated from landfill soil, and after Gram staining, 77% of the isolates are Gram-positive and only 33% are Gram-negative. The result of precipitation examination showed that the bacterial isolates sp. 32, sp. 9, and sp. 20 precipitated 0.129 g, 0.126 g and 0.105 g of calcium carbonate, respectively, after incubation for 7 days in broth medium added with urea and calcium. The bacterial isolates have a close relationship, which is characterized by the magnitude of the coefficient of more than 70%. Therefore, the isolated and tested bacteria having the ability to form calcium carbonate from local soil Riau derived from a group of bacteria, which has a potential to be developed as a mixture of concrete. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 5737  
STUDI PERTUMBUHAN DAN DEGRADASI FENOL OLEH KULTUR TUNGGAL AKTINOMISETES DARI TANAH GAMBUT Tiara Elsita Masni; Tetty Marta Linda; Bernadeta Leni Fibriarti
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2866.46 KB) | DOI: 10.15408/kauniyah.v13i1.12854

Abstract

AbstrakFenol adalah senyawa organik yang bersifat toksik dan larut dalam air, sehingga mudah menimbulkan pencemaran pada perairan dan menurunkan kualitas air. Penelitian ini bertujuan untuk melihat potensi tiga isolat aktinomisetes asal tanah gambut Riau dalam Minimal Salt Medium yang mengandung fenol pada konsentrasi 0 ppm, 200 ppm, 400 ppm, dan 600 ppm serta mengetahui kemampuan aktinomisetes dalam mendegradasi fenol pada konsentrasi 600 ppm menggunakan metode folin ciocalteau. Potensi pertumbuhan isolat L121, L18, L11 menunjukkan total populasi tidak berbeda nyata dengan penambahan 400 ppm dan 600 ppm fenol, tetapi berbeda nyata terhadap 0 ppm dan 200 ppm fenol. Potensi pertumbuhan tertinggi terdapat pada isolat L121 dan terendah pada isolat L11. Kemampuan degradasi  fenol oleh  masing-masing isolat adalah  L121 sebesar 570,80 ppm (95%),  L18 sebesar 218,85 ppm (36%)  dan L11 sebesar  97,21 ppm (16%)  dari konsentrasi fenol awal 600 ppm pada Minimal Salt Medium. Isolat aktinomisetes ini berpotensi dikembangkan untuk penanggulangan pencemaran di lingkungan.Abstract Phenol is an organic compound is toxic and easily soluble in water so easy to cause pollution in a waters such as water quality degradation. The aim of this research is to see the potential of three isolates of actinomycetes from Riau peat soil in Minimal Salt Medium containing phenol concentration 0 ppm, 200 ppm, 400 ppm and 600 ppm and to know the ability of actinomycetes in degradation of phenol at the concentration of 600 ppm using folin ciocalteu. The growth potential of L121, L18, L11 isolates showed the total population was not significantly different with the addition of 400 ppm and 600 ppm of phenol but significantly different from 0 ppm and 200 ppm of phenol. The highest growth potential was found in L121 isolate and lowest in L11 isolate. The degradation ability of phenols by each isolate was L121 570.80 ppm (95%), L18 218.85 ppm (36%) and L11 was able to degrade phenol 97.21 ppm (16%) from the initial phenol concentration of 600 ppm at Minimum Salt Medium.These actinomycetes have the potential to be developed for the overcome of pollution in the environment.
Co-Authors , HERLINDA , TABRI Aini, Diah Nurul Amanda, Dea Anggraini, Yeyen Septia Atria Martina Azzahra, Fhatya Berliansyah, Azizul Berlyansah, Azizul BERNADETA LENI FEBRIANTI, BERNADETA LENI Binar Robinson M Cici Sri Wahyuni Dedi Futra Defani, Syafiqah Amirah Delita Zul Dessy Sari Mulfa Diah Nurul Aini Dwi Wahyuni Erwin Erwin ERWINA JULIANTARI, ERWINA Fauzi, Tiara Nurfadila Febriarti, Bernadeta Leni Fukuhara, Shafira Keiko Hanifa Hanifa Hanifa, Hanifa Herwanda, Fadia Rezika Husadha, Azura Imelda Wardani L. FIBRIARTI, BERNADETA LAMISSI NAPITUPULI Lazuardi Umar Liska Chairani Harahap MAWARTI, INDAH Maya Sari Mira Rianda Monita Olivia Mufidah Dwi Suci Ningsih Muhammad Rinaldi Mulfa, Dessy Sari Nainggolan, Leonardus Riski Nani Anggraini Nery Sofianti Nery Sofiyanti Nila Veronika Nilandra, Namira Ratu Ninik Nihayatul Wahibah Novalita Dwi Fanny Pangestu, Adjie PESRITA, ANALIA Purba, Agnes Tasya Qosim, Muhammad Abdullah Rahayu, Sri Jumaliza Rahmah Agnissah Rahmani, Syauqi Susana Rahmondia N. Setiadi Ramadhan, Dhea Amanda Ramadhan, Ilmi Fajar Rati, Yolanda Ratna Jelita REGIANA SINTAWATI, REGIANA Respati, Reno Widi Rini, Ari Sulistyo Rini, Ari Sulistyo Rizki Amalia RODESIA MESTIKA ROZA Rodesia Mustika Roza Rosandi, Vira Annisa Saberina Hasibuan Safrida Dwiningsih Sari, Avilia Dayang Sembiring, Rinawati Sezhianindi Firiola Silvera Devi Sri Ramadhani Syahgiri, Aldiyan Syauqi Susana Rahmani Tiara Elsita Masni Vanda Julita Julita Vanisa Sri Elvani Wahyu Lestari Wahyu Lestari Wahyu Lestari Wahyuliyanti, Wahyuliyanti Wahyuni, Levita Wati, Andini Saras Wijayanti, Indriani Windi Dona Fitri Fitri Yanuar Hamzah Yanuar Yanuar, Yanuar Yuliati, Rola Zahara, Latifah Zia, Khaleda Zia