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Local Wisdom of The Larung Tumpeng Tradition in Telaga Sarangan : A Resource for Learning Biodiversity and Developing Argumentation Skills Catur Wardeny; Rinie Pratiwi Puspitawati; Lisa Lisdiana
Jurnal Kependidikan: Jurnal Hasil Penelitian dan Kajian Kepustakaan di Bidang Pendidikan, Pengajaran dan Pembelajaran Vol 11, No 1 (2025): March
Publisher : Universitas Pendidikan Mandalika (UNDIKMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jk.v11i1.13585

This research aims to develop an e-book that explores biodiversity through the lens of the Telaga Sarangan Larung Tumpeng tradition, integrating local wisdom to enhance students' argumentation skills. Utilizing a development research method, the study follows the ADDIE framework, which consists of analysis, design, development, implementation, and evaluation phases. The data analysis technique employs validation tests and readability assessments for the e-book development outcomes, analyzed using a qualitative descriptive approach. The research utilized an e-book validation sheet evaluated by two lecturers—one an expert in content and the other in educational methods. Additionally, a readability test was conducted using Fry graphs. The validation results yielded an average score of 3.9, categorizing the e-book as very valid. This evaluation took into account various aspects such as presentation validity, content validity, and language validity. Additionally, the practicality of the e-book is evaluated through a readability test, which indicates a reading level appropriate for tenth-grade students. The e-book achieved a readability level of 12, classified as very positive and suitable for its intended audience. The study clearly demonstrated that the e-book focused on biodiversity, grounded in the local wisdom of the Larung Tumpeng tradition at Telaga Sarangan, is both valid and practical for enhancing the argumentation skills of X-grade high school students.

Effectivity Analysis of Offline and Online Learning Systems on Mendel’s Law of Inheritance Topic in High School Grade 11 Students Addabiyah, Sayyidatul; Lisdiana, Lisa
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 13 No. 1 (2024)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/bioedu.v13n1.p207-217

This research aims to compare the effectiveness of offline and online learning systems on inheritance topic in grade 11 high school students of MAS Kanjeng Sepuh in terms of student learning outcomes, student responses, and management of learning process. Online learning carried out by using the Whatsapp, Google Classroom and Google Meet applications, while offline learning was carried out face-to-face in the classroom. The design of this study used a Quasi Experimental Design with a Non-equivalent control group design type. This research was conducted at MAS Kanjeng Sepuh with a total of 24 students from class 11 IPA 1 and 11 IPA 2. The sampling technique used was saturation sampling. The results of the research showed that there were no significant differences in learning outcomes before and after online learning, but significant differences were found in learning outcomes before and after offline learning. Students showed more positive responses to the offline learning system than the online learning system. In addition, the management of learning process in online and offline learning was in the good and excellent categories so that they were equally effective. Therefore, it can be concluded that the offline learning system was more effective than the online learning system in terms of learning outcomes and student responses. Meanwhile, in term of the management of learning process, offline and online learning systems were equally effective. Both online and offline learning systems have their advantages and disadvantages that affect the effectiveness of learning, so more attention is needed to minimize the disadvantages of each learning system, especially online learning systems so that the learning process can be more optimal. Keywords: online learning, offline learning, learning outcomes, student responses, high school

Development of Electronic Worksheet Based on Scientific Approach to Train Critical Thinking Skills on Membrane Transport Topic Anggraini, Lusiana; Lisdiana, Lisa
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 13 No. 1 (2024)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/bioedu.v13n1.p1-11

Eligibility of Electronic Student Worksheet Based on Two Stay Two Stray to Train Analysis Thinking Skills in Inheritance Topic Meilinda, Alfina; Lisdiana, Lisa
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 13 No. 1 (2024)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

BIODIVERSITY OF CELLULOLYTIC BACTERIA ISOLATED FROM FERMETODEGE FOR RUMINANTS Isnawati, Isnawati; Lisdiana, Lisa; Asri, Mahanani Tri; Trimulyono, Guntur
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.1904

ARTICLE HIGLIGHTS- Fermetodege as a source of cellulolytic bacteria that degrade cellulosic materials.- Enhances ruminant feed by improving palatability and digestibility.- Utilizes water hyacinth, reducing invasive plant spread and supporting ecosystems.- Bioprospecting reveals new bacteria for effective fermentation starter development.ABSTRACTFermetodege is a name of fermented ruminant feed produced from free heavy metals water hyacinth, rice bran, and corn cobs. Its quality, as well as the fermentation process, are affected by numerous factors, such as the diversity, evenness, and dominance of indigenous cellulolytic bacterial species. A proper understanding of these influential factors is needed to improve the quality of the fermented feed. Therefore, this study aims to evaluate the diversity, evenness, and dominance of cellulolytic bacteria isolated from fermetodege. The cellulolytic activity of the isolates was tested by observing their growth on the carboxymethylcellulose (CMC) media. Isolates with cellulolytic activity formed a clear zone after the colonies were soaked with 0.1% Congo red and rinsed with 1 M NaCl. The Shannon-Weiner's diversity and evenness indexes, as well as Simpson's species dominance, were then calculated. The result showed that the Shannon-Weiner’s diversity index ranged from 0.6849 to 1.8173, and it was categorized as medium. The evenness index was between 0.7778 and 0.9983, which indicates a stable distribution of species. Meanwhile, the Simpson’s species dominance ranged from 0.1835 to 0.5082, which implies that none of the species was dominant. These results show that fermetodege is potentially a source of bacterial isolates and can be used as a fermentation consortium starter.

Local Wisdom of The Larung Tumpeng Tradition in Telaga Sarangan : A Resource for Learning Biodiversity and Developing Argumentation Skills Wardeny, Catur; Puspitawati, Rinie Pratiwi; Lisdiana, Lisa
Jurnal Kependidikan : Jurnal Hasil Penelitian dan Kajian Kepustakaan di Bidang Pendidikan, Pengajaran, dan Pembelajaran Vol. 11 No. 1 (2025): March
Publisher : LPPM Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jk.v11i1.13585

This research aims to develop an e-book that explores biodiversity through the lens of the Telaga Sarangan Larung Tumpeng tradition, integrating local wisdom to enhance students' argumentation skills. Utilizing a development research method, the study follows the ADDIE framework, which consists of analysis, design, development, implementation, and evaluation phases. The data analysis technique employs validation tests and readability assessments for the e-book development outcomes, analyzed using a qualitative descriptive approach. The research utilized an e-book validation sheet evaluated by two lecturersâ€”one an expert in content and the other in educational methods. Additionally, a readability test was conducted using Fry graphs. The validation results yielded an average score of 3.9, categorizing the e-book as very valid. This evaluation took into account various aspects such as presentation validity, content validity, and language validity. Additionally, the practicality of the e-book is evaluated through a readability test, which indicates a reading level appropriate for tenth-grade students. The e-book achieved a readability level of 12, classified as very positive and suitable for its intended audience. The study clearly demonstrated that the e-book focused on biodiversity, grounded in the local wisdom of the Larung Tumpeng tradition at Telaga Sarangan, is both valid and practical for enhancing the argumentation skills of X-grade high school students.

Detection of Porcine Contamination in Beef Meatballs from Gedangan District Using PCR–RFLP Suryadi, Gian Firdaus Putra; Astuti, Rahma Windi; Fitriani, Lilis Nur; Lisdiana, Lisa
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49813

Meatball is one of the popular meat dishes in Indonesia, especially in Sidoarjo. The sale of meatballs in Sidoarjo is carried out by Micro, Small, and Medium Enterprises (MSMEs), with 330 of the total 11,114 micro-traders selling meatballs. However, there is a risk of pork being adulterated into meatballs. This is a serious problem because Indonesia, especially Sidoarjo, is a Muslim-majority city with 95.5% of the total population of 2 million. Therefore, the purpose of this article is to conduct field checks to determine whether or not pork contamination is present in meatballs in Sidoarjo. Sampling locations were determined using a stratified random sampling method, which found that eight of the 15 villages, including Karangbong, Tebel, Sruni, Keboananom, Keboansikep, Gedangan, Ketajen, and Sawotratap Villages, have a significance value greater than the alpha value (0.05). Therefore, eight of them were used as sampling locations. One sample was purchased from each of the eight chosen villages, named S1, S2, S3, S4, S5, S6, S7, and S8. Meanwhile, the possibility of porcine contamination in beef meatball samples was analyzed using the Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method with the restriction enzyme BsaJI. This study found that all eight samples and the negative control DNA amplicons were not cleaved by the BsaJI enzyme. Meanwhile, the positive control DNA amplicon was cleaved into 3 fragments. It was indicated that all samples and the negative control DNA amplicons were free of pork DNA contamination. Despite the anomaly, it can be concluded that all meatball samples bought from Gedangan District are not contaminated with pork. By demonstrating that meatballs from Sidoarjo are free from pork DNA, this study serves as an additional halal food monitoring in Sidoarjo. It reinforces public trust in local MSME food products.

IDENTIFICATION OF BACTERIA FROM FOOD COURT WASTEWATER BASED ON 16S rRNA GENE SEQUENCES Elasari , Indah Nur; Abdillah , Rihan Ali; Dewa , Satria Prasna; Widjaja, Jafni Amanda Kinarossa; Lisdiana, Lisa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13541

Environmental safety and public health are greatly influenced by the microbiological elements such as bacterial community, where these organisms can be used as indicators of environmental quality and sources of potential biological hazards. The identification of bacteria in wastewater is an important aspect of environmental microbiology studies. The purpose of this study was to identify the bacterial species in food court wastewater at State University of Surabaya (UNESA) Campus 1. The methods used were exploratory, including bacterial cultivation, DNA extraction, quantitative and qualitative DNA analysis, amplification of 16S rRNA gene fragments, sequencing, and phylogenetic tree reconstruction. From phylogenetic analysis, five test isolates were related to the families Enterobacteriaceae, Aeromonadaceae, Chromatiaceae, and Halomonadaceae. Isolates BU4 and BU5 were most closely related to genus Aeromonas with a similarity of 98.60% and 97.27%, respectively, while isolate BU3 was closely related to genus Klebsiella with a similarity of 96.91%. Meanwhile, isolates BU1 and BU2 cannot yet be identified into a bacterial genus. The results of this study provide important information regarding the prediction of pathogenic bacteria types, which can serve as the basis for recommending further action to prevent disease, improve water quality, and increase the sterility of the food court area.

IDENTIFICATION OF BACTERIA FROM FOOD COURT WASTEWATER BASED ON 16S rRNA GENE SEQUENCES Elasari , Indah Nur; Abdillah , Rihan Ali; Dewa , Satria Prasna; Widjaja, Jafni Amanda Kinarossa; Lisdiana, Lisa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13541

Environmental safety and public health are greatly influenced by the microbiological elements such as bacterial community, where these organisms can be used as indicators of environmental quality and sources of potential biological hazards. The identification of bacteria in wastewater is an important aspect of environmental microbiology studies. The purpose of this study was to identify the bacterial species in food court wastewater at State University of Surabaya (UNESA) Campus 1. The methods used were exploratory, including bacterial cultivation, DNA extraction, quantitative and qualitative DNA analysis, amplification of 16S rRNA gene fragments, sequencing, and phylogenetic tree reconstruction. From phylogenetic analysis, five test isolates were related to the families Enterobacteriaceae, Aeromonadaceae, Chromatiaceae, and Halomonadaceae. Isolates BU4 and BU5 were most closely related to genus Aeromonas with a similarity of 98.60% and 97.27%, respectively, while isolate BU3 was closely related to genus Klebsiella with a similarity of 96.91%. Meanwhile, isolates BU1 and BU2 cannot yet be identified into a bacterial genus. The results of this study provide important information regarding the prediction of pathogenic bacteria types, which can serve as the basis for recommending further action to prevent disease, improve water quality, and increase the sterility of the food court area.

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