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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Ilmu Pertanian Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri JURNAL AGROTEKNOLOGI AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan jurnal Ilmu Pertanian (Agricultural Science) Jurnal Penelitian Tanaman Industri (Littri) Agrokompleks
SEDYO HARTONO
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Incidence of Soybean Mosaic Disease in East Java Province Andayani, Wuye Ria; Sumardiyono, Y. B.; Hartono, Sedyo; Yudono, Prapto
AGRIVITA Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i1.34

Abstract

The objectives of this study were: 1) to identify the mosaic symptom severity and the incidence the virus and relate these to soybean yield reduction is four regions of East Java; 2) diagnostic of the symptom using Indirect ELISA, RT-PCR and electron microscope observation. Results from experiments indicated that soybean plants infected with SMV and CMMV, alone or in combination produced mosaic symptom. Incidence of the virus, as judged by symptomatology, ranged from mild to severe infection the percentage of plants being from 13.42-30.10%. Soybean plants with mosaic symptom caused SMV from an early stage of development (14-28 days after planting). Soybean mosaic virus belongs to the virus family Potyviridae. Specific DNA fragment of 1687 bp was successfully amplified from soybean infected by SMV isolate Ngawi, Madiun, Magetan, and Ponorogo. Specific DNA fragment of 1385 bp was successfully amplified from SMV by CI coding region. The mosaic symptom on soybean plant (28-42 days after planting) caused CMMV. Flexious virus particle 650 nm in length was observed on electron microscope. It caused local lesions on Chenopodium amaranticolor, but not detected by I ELISA with antiserum SMV and RT-PCR with universal primer. Keywords: SMV, CMMV, I.ELISA, RT-PCR.
Co-Authors Alvina Clara Giovanni Aminatun Munawarti Anak Agung Gde Raka Swastika ANDI KHAERUNI Ani Widiastuti Argawi Kandito Argawi Kandito Argawi Kandito Arman Wijonarko Asmar Hasan Astuti, Suryani Titi Azizah Ridha Ulilalbab Botek, Muhammad Budi Setiadi Daryono BUDI SETIADI DARYONO Cahyo Hertanto Christanti Sumardiyono Christina Retna Handayani Deden Sukmadjaja Dewi Rahmitasari Didit Setiyawan Dini Wahyu Kartika Sari Efendi, Darda Emerensiana - Uge Emerensiana Uge Erna Anastasia Esti Prasetya Ningrum Fariha Wilisiani Fitri Kusumaningrum Gede Suastika GUSNAWATY HS, GUSNAWATY hasdiana, hasdiana Helina, Selvi Heri Widarta Hisein, Siti Anima I Dewa Nyoman Nyana I Nyoman Widiarta Ika Roostika Ika Roostika Ismiyatuningsih Ismiyatuningsih Jun Kobayashi La Ode Santiaji Bande Ma'unah Ambarwati Mery Windarningsih Muhammad Muhsin MUHAMMAD TAUFIK Mustika Ajeng Kartini Putri Pertiwi Nanda Kusumandari Nasrun Nasrun NOOR AIDAWATI Nuri Yusmarlita Nurjanah Nurjanah Praptana, R. Heru Prapto Yudono Prapto Yudono PURNAMA HIDAYAT R. Heru Praptana Rahayu Mallarangeng, Rahayu Rahma Ayu Priani Resti Fajarfika Retno Mastuti Rizko Hadi, Rizko Saipul Abbas Sekar Utami Putri Selvi Helina Serafinah Indriani Siwi Indarti Soesamto Somowiyarjo Somowiyarjo, Susamto Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari SRI SULANDARI Sri Sulandari Sri Sulandari Sri Sulandari Subandi Sukamto Sukamto Sumardiyono, Y. B. Suprihanto, Suprihanto susamto - somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Sumowiyarjo Syair, Syair Tomohide Natsuaki Tri Harjaka Tri Joko Tri Joko Tri Maruto Aji Tri Maruto Aji Tri Retno Widyastuti Triharso Triharso Trisnani Alif Triwidodo Arwiyanto Tuty Arisuryanti Ulfa, Nur Isnaini Umi Kulsum Widiarta, I Nyoman Wiwik Endarsih Wuye Ria Andayani Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. B. Sumardiyono Y. B. Sumardiyono Y.M.S. Maryudani Yashanti B. Paradisa Yashanti Berlinda Paradisa YB Sumardiyono