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All Journal Jurnal Teknologi Dan Industri Pangan Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Jurnal Akademika Biologi Jurnal Biologi Universitas Andalas Biosaintifika: Journal of Biology & Biology Education Journal of Applied Food Technology Berkala Bioteknologi Advance Sustainable Science, Engineering and Technology (ASSET)
Arina Tri Lunggani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Education Electrical & Electronics Engineering Energy Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Other

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Tingkat Kontaminasi Tembaga dan Timbal pada Perairan Pelabuhan Tanjung Emas Semarang Purnomo, Eko; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto; Lunggani, Arina Tri
Jurnal Ilmu Lingkungan Vol 23, No 5 (2025): September 2025
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.23.5.1378-1384

Abstract

ABSTRAKPencemaran polutan logam berat tembaga (Cu) dan timbal (Pb) di perairan Pelabuhan Tanjung Emas Semarang (PTES), diakibatkan oleh dampak aktivitas pelabuhan, pelayaran, proses industri dan buangan limbah domestik perkotaan. Hal ini berdampak negatif pada keberlanjutan ekosistem akuatik dan masyarakat setempat, sehingga diperlukan kajian terkait pencemaran logam berat tembaga dan timbal di perairan PTES. Penelitian ini bertujuan untuk memberikan referensi mengenai kondisi terkini terkait tingkat pencemaran logam Cu dan Pb di perairan PTES, serta membandingkan tingkat cemaran terhadap standar kualitas lingkungan. Metode penelitian ini mengukur konsentrasi Cu dan Pb pada lapisan sedimen dan perairan di tiga stasiun sampling. Metode pengukuran logam Cu dan Pb menggunakan metode Atomic Absorption Spectroscopy (AAS). Data konsentrasi logam berat yang diperoleh kemudian dibandingkan dengan standar kualitas sedimen (Sediment Quality Guidelines, SQG) dari U.S. Environmental Protection Agency (US EPA) untuk sampel sedimen dan standar PP RI No. 22 Tahun 2021 untuk sampel air, untuk mendapatkan status cemaran Cu dan Pb. Berdasarkan hasil kajian, status cemaran Cu dan Pb di sedimen berada dalam kategori tidak tercemar (SQG US EPA). Dimana konsentrasi logam berat tertinggi berada pada stasiun 1 dengan konsentrasi Cu yaitu 10.99 ppm dan konsentrasi Pb yaitu 0.03 ppm. Sedangkan status cemaran Cu dalam perairan berada dalam kategori aman dengan konsnetrasi Cu pada semua stasiun yaitu 0.01 ppm. Sementara itu, status Pb dalam perairan berada di atas baku mutu dan tercemar berdasarkan PP RI No. 22 Tahun 2021, dengan konsentrasi Pb pada ketiga stasiun yaitu 1.00 ppm, 1.20 ppm dan 1.60 ppm. Konsentrasi logam berat terutama Pb di perairan dapat mempengaruhi ekosistem akuatik, prioritas penanganan dan penelitian lebih lanjut sangat diperlukan untuk mengupayakan teknologi remediasi logam berat di perairan PTES. ABSTRACTThe contamination of heavy metals, particularly copper (Cu) and lead (Pb), in the waters of Tanjung Emas Port, Semarang (PTES), is primarily attributed to maritime operations, industrial processes, and domestic waste. This pollution poses significant threats to the sustainability of aquatic ecosystems and the well-being of surrounding communities. Therefore, an in-depth assessment of Cu and Pb pollution in PTES waters is urgently needed. This study aims to provide an updated reference on the current levels of Cu and Pb contamination in the PTES waters and to compare these concentrations against established environmental quality standards. The research methodology involved measuring Cu and Pb concentrations in both sediment and water samples collected from three stations. The levels of Cu and Pb were determined using AAS. The obtained heavy metal concentration data were compared against the Sediment Quality Guidelines (SQG) set by the US EPA for sediment samples, and the Indonesian Government Regulation (PP R1) No. 22 of 2021 for water samples. The study revealed that the contamination status of Cu and Pb in sediments was classified as "unpolluted" based on the SQG US EPA. The highest concentrations were recorded at Station 1, with Cu at 10.99 ppm and Pb at 0.03 ppm. Copper contamination in water samples from all stations was found to be within safe limits. However, Pb concentrations in water samples exceeded the quality standards set by PP RI No. 22 Tahun 2021, indicating a polluted status. Pb concentrations were recorded at 1.00 ppm, 1.20 ppm, and 1.60 ppm across the three sampling stations, respectively. The elevated levels of Pb in the aquatic environment could significantly impact the local ecosystem, highlighting the urgent need for prioritizing mitigation measures and advancing further research for developing and implementing effective heavy metal remediation technologies in the waters of PTES.
Antibacterial Activity of Freshwater Sponge Oncosclera asiatica Against Escherichia coli and Staphylococcus aureus Wulandari, Dyah; Dewantoro, Giwang; Lunggani, Arina Tri; Suprihadi, Agung; Riani, Catur; Setiawan, Edwin; Farikha, Siti Lutfiatul; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.120-129

Abstract

Freshwater sponges are animals from the Porifera phylum that live in freshwater. The sponge used is Oncosclera asiatica was taken from Kali Porong, East Java. Seventeen isolates of bacteria have been obtained from isolation. Antibacterial potential testing was performed by paper disc inhibition assay using Escherichia coli and Staphylococcus aureus as pathogenic bacteria and amoxicillin as a positive control. The antibacterial activity test showed that four isolates have the potential activity. The isolates with the highest inhibition zones were identified using a 16S rRNA.The results of BLAST showed isolate number 2 was Pseudomonas moraviensis with 99.51% similarity. The phylogenetic tree analysis was build using the MEGA X program. The results of the phylogenetic tree analysis showed that P.moraviensis had a bootstrap value of 100% with a  genetic distance value of 0.001. P. moraviensis isolates screened for the presence of Non-Ribosomal Peptide Synthetase (NRPS) gene by A2gamF and A3gamR primers. The amplification result from NRPS gene showed positive meaning that P.moraviensis genome contained NRPS gene.
Characterization And Screening Of Protease, Amylase, And Cellulase From Phylloplane Fungi Isolates Of Avicennia marina (Forssk.) Vierh. Mahardhika, Wahyu Aji; Ramadhany, Warih; Lunggani, Arina Tri
Jurnal Biologi Universitas Andalas Vol 9 No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.54-59.2021

Abstract

Mangrove (Avicennia marina) is an important mangrove species, because it has many benefits for human life, including in the ecological scope as a source of food and medicine. These plants can also be used as a source of symbiont mold isolates which can be developed as an alternative to produce bioactive compounds, one of which is enzymes. Enzymes are protein compounds that can catalyze all chemical reactions in biological systems. This study aims to determine whether mangrove symbiont fungi (A. marina) can produce amylase, protease and cellulase enzymes. This research was conducted using a method based on enzyme activity, namely amylase activity with lugol iodine staining, protease activity and cellulase activity with congo red staining. on agar media enriched with 1% skim milk, 1% starch and 1% CMC. The results showed that 4 isolates were able to show potential enzymatic activity to be developed as agents for enzyme production.
Characterization of Yellow Pigmented Bacteria Associated with Gracilaria sp. Lunggani, Arina Tri; Purwantisari, Susianna; Jannah, Siti Nur
Advance Sustainable Science, Engineering and Technology Vol 2, No 2 (2020): May-October
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v2i2.7041

Abstract

Research on the kinship analysis of endophytic bacterial  isolated from Gracillaria sp has been carried out. The presence of bacteria associated with Gracilaria sp. has enabled the use of these bacteria as a source of new bioactive compounds, such as biopigments. The research aims to isolated bacteria from Gracilaria sp., screened their symbiont bacteria that could potentially produce pigments. Sampling Gracilaria sp. conducted in the waters of the Island of  Karimunjawa, Jepara. Furthermore, bacterial isolation was carried out, screening for pigment-producing bacteria and 16S rRNA sequence analysis. Research result showed that the symbiont bacteria isolate TK 373 produced consistent pigments after several regenerations, in several types of growth media incubated at room temperature. The results of 16S rDNA identification showed that the TK 373 isolate had the closest relationship with  Pseudoalteromonas sp. with  98.72 % homology.
Glutamic Acid Production by Lactic Acid Bacteria Isolated from Indonesian Fermented Food Salted Mustard Greens and Dangke Cheese Indahsari, Silvana Nurulfauziyyah; Jannah, Siti Nur; Lunggani, Arina Tri
Jurnal Teknologi dan Industri Pangan Vol. 36 No. 2 (2025): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2025.36.2.195

Abstract

Glutamic acid is an additive compound widely added to food to enhance the savory taste (umami). Lactic acid bacteria (LAB) are included in Generally Recognized as Safe (GRAS) and have the potential to produce various metabolite compounds, including glutamic acid, through fermentation. LAB can be isolated from salted mustard greens and dangke cheese. This study aimed to analyze the effect of LAB isolate types and fermentation time on the production of glutamic acid, glutamic acid profiling, and molecularly identify the LAB genus that produces the highest glutamic acid based on the 16S rRNA gene. The fermentation process of LAB was carried out using four selected isolates: D16, D15, S4, and S15, which were isolated from salted mustard greens and dangke cheese. Each isolate was incubated for five different incubation times: 0, 12, 24, 48, and 72 h. The identification of glutamic acid was carried out using the Thin Layer Chromatography (TLC) method, its quantification by spectrophotometry, and profiling by High-Performance Liquid Chroma-tography (HPLC). In addition, molecular identification of the highest-producing LAB isolate was conducted based on the 16S rRNA gene. The results showed that isolate S4 from salted mustard greens produced the highest glutamic acid after 48 h, with 670.05 mg/L and a total glutamic acid of 0.23% (w/w) based on HPLC results. Isolate S4 is known to be molecularly similar to the Pediococcus pentosaceus species. Local LAB isolates from salted mustard greens and dangke cheese can produce glutamic acid that can be used to enhance the taste of fermented foods.
Co-Authors Aninditia Sabdaningsih Anto Budiharjo Budi Raharjo Budihardjo, Kadarwati Cahyaningrum, Emi CATUR RIANI Choirunnisaa, Nadia Maharani Jasmine Dani Ika Purwaningsih Dewantoro, Giwang Dian Prambodo, Ashar Kurnia Diani Kurniawati Dion, Romario Dyah Wulandari Edwin Setiawan Eko Purnomo Endang Kusdiyantini Endang Kusdiyantini Enny Fachriyah Farikha, Siti Lutfiatul Hanifa, Yumna Rahmadias Hapzi Ali Hermin Pancasakti Kusumaningrum Indahsari, Silvana Nurulfauziyyah Khabib Khasan Alfaridhi, Khabib Khasan Khodijah Baharun Mahardhika, Wahyu Aji Mahardika, Wahyu Aji MG Isworo Rukmi Muhammad Amal Nurhakim, Muhammad Amal Nur Jannah, Siti Purwantisari, Susianna Qoys Naufal, Mochammad Fa’iq Raden Mohamad Herdian Bhakti Ramadhany, Warih Sari, Rachel Dewita Sitepu, Harum Siti Nur Jannah Soni Nugraha Anwar Sri Pujiyanto Susiana Purwantisari Syawal Nurangga Kodhatin Utomo, Adi Budi Whinarsih, Whinarsih Wijanarka Wijanarka Wijanarka, W