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Journal : Journal Of Aquaculture Development and Environment

Detection of White Spot Syndrome Virus (WSSV) of Shrimp (Litopenaeus vannamei) in UPT Pengembangan Budidaya Air Payau Bangil , Pasuruan District , East Java Rima Oktavia Kusuma; Muh. Sulaiman Dadiono; Baruna Kusuma; joni Johanda Putra
Journal of Aquaculture Development and Environment Vol 3, No 2 (2020): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v3i2.3426

Abstract

Vanname shrimp (Litopenaeus vanname) is still a favourite in shrimp culture production in Indonesia, since it has faster growth advantages. However, the White spot syndrome virus (WSSV) attack is still a big threat for farmers, because it can proliferates rapidly and cause mass mortality of 90-100%. The UPT Pengembangan Budidaya Air Payau in Bangil maintains vanname and controls virus infection by detecting through a molecular approach. Detection using the Nested PCR method with IQ 2000 kit with molecular weight marker 848 bp, 550 bp, 296 bp. The sample will be tested positive if the bands that appear are in the size 296 bp and / or 550 bp . The results of the detection in shrimp rearing ponds were negative (-) because only the band 848 bp that showed, it was mean the shrimp free from WSSV infection. Water quality data shows that the shrimp rearing environment is in optimal conditions.
Survival Rate Evaluation of Different Filler Medium of Waterless Live Fish Transportation of African Catfish (Clarias sp.) Broodstock Ayuningtyas Ayuningtyas; Baruna Kusuma; Abdul Rahem Faqih; Yunita Maimunah
Journal of Aquaculture Development and Environment Vol 1, No 1 (2018): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v1i1.1012

Abstract

The supplying of live fish broodstock is routinely delivered by water. However, the traditional transportation of live fish with water results in small volume of transportation. Therefore, waterless transportation with different filler medium of waterless live fish transport is considered an alternative strategy. This research aimed to evaluate the effect of different filler medium of waterless live fish transportation on survival rate of African catfish (Clarias sp.) broodstocks. Brooders with mean weight 116 ±10,25 g were packed at 5 fish/bag for simulated 15h transportation. The bags, each 40x25x15 cm3, were filled with different treatment of medium; Sponge, newspaper, cloth, and sawdust at 3 cm thickness of sponge medium and ½ kg for newspaper, cloth and sawdust medium per bag. One bag without medium was designed as a control. Mortality, temperature, Humidity and weight gain loss were monitored throughout the experiment. After simulation, mortality, pH, DO and temperature were monitored in fiber pond over 14 days. Effect of different treatment of medium was significantly different (p < 0.05) with highest survival (100%) at sponge and cloth medium, and lowest survival (24 %) at sawdust medium. Humidity of newspaper medium and weight gain loss of sawdust medium significantly decreased and no significantly different in temperature parameter after simulated 15h transportation. It was concluded that sponge and cloth medium might be applied as a filler medium for waterless live fish transportation.
The Effect of Duck Manure Fermentation Subtitution for Juvenile Catfish Feed (Clarias sp.) Baruna Kusuma; Esna Dili Novianto
Journal of Aquaculture Development and Environment Vol 1, No 1 (2018): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v1i1.1010

Abstract

Pellet is replaceable with fermented duck manure in the catfish. The effect of substitution pellet feed juvenile catfish with fermented duck manure was investigated. It can be done by fermentation process of duck manure by yeast from traditional food starter called Tape. It contains yeast of genus Aspergilius, Saccharomyces, Candida, Hansenulla, dan Acetobacter bacteria. Catfish diets supplemented with 5% pellet/fish/day (W/W/day). Four treatments diets were 45% (A), 30% (B), 15% (C) and 0% (D) levels of duck manure from total weight and it fed to catfish juvenile (weight 2,75±0,05 g) for 30 days controlled aquaria. The results of this studies found that the recommended diet was diet C and D formulations beacuse it can provide catfish juvenile growth response. Duck manure can replace feed juvenile catfish by fermentation for maximmum 15% from catfish diets.