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All Journal Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Natur Indonesia Journal of Tropical Soils Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BioWallacea Journal of Biological Research Indonesian Journal of Biotechnology

Sebastian Margino

Mikrobiology Laboratory, Faculty of Agriculture, Universitas Gadjah Mada. Yogyakarta, Indonesia.

Author-ID : 315370

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Optimasi Produksi Poli-Î²-Hidroksibutirat (PHB) oleh Bacillus sp. PSA10 Yanti, Nur Arfa; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

A new strain characterized as Bacillus sp. PSA10 was found to produce poly-Î²-hydroxybutyrate (PHB) at concentration of 52.28% (g PHB/g dry cell weight) in shaken flask culture, using sago starch as a carbon source. This research is aimed to determine the optimum culture condition of PHB production Bacillus sp. PSA10 at laboratory scale. Optimization of PHB production was conducted in this research, in terms of inoculum concentration, concentration of the major components in minimal medium, environmental condition and incubation time. The result showed that optimum conditions for the production of PHB by Bacillus sp. PSA10 were achieved at minimal medium (Ramsay medium) with 5% (v/v) inoculum concentration, 2% (w/v) sago starch, 1.0 g/l (NH4)2SO4, 6.7 g/l Na2HPO4.7H2O, and 0 g/l KCl. The optimum environmental conditions were achieved with initial pH 7, temperature 37oC, agitation speed at 150 rotary per minute (rpm) and the best of incubation time was 48 hour. Under this optimum condition, the maximum PHB production by Bacillus sp. PSA10 increased from 52.28% to 71.35% (g PHB/g dry cell weight) at 48 hour cultivation. Therefore, Bacillus sp. PSA10 is potential to apply for PHB production from sago starch at industrial scale.

Kondisi Optimum untuk Produksi Kitinase dari Streptomyces Rkt5 dan Karakterisasi pH dan Suhu Enzim Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Chitinase is chitin degrading enzyme which is produced by Streptomyces Rkt 5 is isolated microorganism from peanut rhizosfer. This enzyme and its microorganism can be used in many agricultural, medicine and industrial purposes. The aim of the research was to find out the optimum condition for production of chitinase and to characterize of pH and temperature to chitinase activity. Optimalizing production the research had 4 treatments. The optimum conditions were achieved at mineral liquid medium containing with chitin 0,2% (w/v) as inducer, 10% (v/v) inoculum, pH 7 and 48 hours incubation. The crude enzyme was partially purified by salting out with 70% ammonium sulfate resulted in 3.31 time more purity enzyme than the crude one. This enzyme had maximum activity at 50oC and pH 5.5.

Isolasi Dan Karakterisasi Bakteri Asam Laktat Dari Usus Udang Penghasil Bakteriosin Sebagai Agen Antibakteria Pada Produk-Produk Hasil Perikanan Romadhon, Romadhon; Subagiyo, Subagiyo; Margino, Sebastian
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 8, No 1 (2012): Jurnal Saintek Perikanan
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Lactid Acid bacteria are capable of fermenting sugars or carbohydrates to produce lactic acid in large numbers. The characteristics of lactic acid bacteria in general are cells reacted positively to the Gram stain, catalase react negatively and do not form spores. And fermentation of glucose would result Iaktat acid. Isolation of lactic acid bacteria carried in the intestines of three species of shrimp that Tiger Shrimp (Penaeus monodon), shrimp Seker (Metapenaopsis sp.), Guts White shrimp (Penaeus merguensis). Selection of bacteriocin -producing lactic acid bacteria were in a qualitative and semi-quantitative (pitting) in MRS medium. Selected isolates were identified by morphological characteristics, biochemical, physiological, A number of 209 isolates were isolated from three species of shrimp. The results of the qualitative selection there were 54 isolates produced bacteriocins. The results of the quantitative selection of 54 isolates produced 24 fine isolates. From the 24 isolates there are 2 isolates seed SFE-7 (33) and P12A (25). Characteristics of seed isolates had morphological characteristics of spherical shape, cell arrangement tetrad, gram positive, negative motility. While the results of the biochemical approach to gram-negative, catalase negative, homofermentative (no gas in the fermentation of glucose), can ferment D-Galactose, D-Glucose, D-mannose, D-lactose. Physiological approaches to isolate growth SFE-7 (33) and P12A (25) can be generated that both isolates able to live in the range of 4 ° C to 50 ° C, in the range of pH between 4-10, and able to grow at levels of 5-10% NaCl . Based on these characteristics can be inferred featured isolates was Pediococcus acidilactici Key words: Lactid Acid Bacteria, intestine of shrimp, Pediococcus acidilactici

Isolation and Selection of Rhizobium Tolerant to Pesticides and Aluminum from Acid Soils in Indonesia Martani, Erni; Margino, Sebastian; Indradewa, Didiek; Supriyo, Agus
JOURNAL OF TROPICAL SOILS Vol. 16 No. 1: January 2011
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2011.v16i1.47-54

Application of Rhizobium as inoculum in acid soil requires specific characters, namely high tolerance to pesticide residues, soil acidity, and high concentration of Aluminum. This study was conducted to isolate Rhizobium having these characters. Inspite of acid soils from Kalimantan, Sumatra, Sulawesi and Java; root nodules of legumes planted in those regions were used as source of isolates. Rhizobial isolation was done using direct isolation andenrichment technique. A paper disc diffusion technique was used in selecting tolerance to pesticides. The selected isolates were examined the tolerance to pH, Al, and ability to form root nodule with soybean. From soil analysis, it could be seen the correlation between pH value and Al concentration. It means that the lower pH value the higher Al concentration. The number of Rhizobium isolates and its tolerance to paraquat was depended on soil type. From 173 strains of isolated Rhizobium, 24 strains were tolerance to pesticides and Aluminum. They were able to grow in wide range of pH, namely 3 – 8, or some of them in 5 - 8. Around 92% of the selected bacteria could form root nodules with soybean plant in different number and size. Hopefully, these isolates can be applied in the pesticide polluted agricultural lands, especially in acid soils with high concentration of Al, and it can also increase soybean production.Keywords: Acid soils, aluminium, Rhizobium, pesticides

Co-Authors Agus Supriyo, Agus Agustinus Joko Nugroho, Agustinus Joko ANDRIESSA PRAMESWARA Bostang Radjagukguk Chatarina Behar, Chatarina Elisa Nurnawati Erni Martani Indradewa, Didiek Kurniawan Wibowo, Kurniawan LANGKAH SEMBIRING Medhina Magdalena, Medhina Ngadiman ., Ngadiman Nurhayani H. Muhiddin, Nurhayani H. Romadhon Romadhon Sari, Rarat Mulat Sarto Selvi Marcellia Subagiyo Subagiyo Widya Asmara Yurnaliza, Yurnaliza

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