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All Journal SURYA AGRITAMA - Fakultas Pertanian Jurnal Ilmu dan Teknologi Hasil Ternak SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Riset Agribisnis dan Peternakan Jurnal Ilmiah Fillia Cendekia Surya Agritama: Jurnal Ilmu Pertanian dan Peternakan Jurnal Sains Peternakan Nusantara Kandang : Jurnal Peternakan Taroa : Jurnal Pengabdian Masyarakat Prosiding University Research Colloquium Jurnal Ilmiah Ilmu-Ilmu Peternakan Prosiding Seminar Nasional Hasil Penelitian dan Pengabdian Kepada Masyarakat
Rinawidiastuti Rinawidiastuti
Universitas Muhammadiyah Purworejo
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Isolasi dan Identifikasi Molekuler Bakteri Asam Laktat dari Susu yang berpotensi sebagai Pangan Fungsional: Review Literatur: Isolation and Molecular Identification of Lactic Acid Bacteria from Milk with Potential as Functional Food: Literature Review Roisu Eny Mudawaroch; Rinawidiastuti Rinawidiastuti
Jurnal Riset Agribisnis dan Peternakan Vol. 11 No. 1 (2026): Jurnal Riset Agribinis dan Peternakan
Publisher : Universitas Muhammadiyah Purworejo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37729/jrap.v11i1.7613

Abstract

Lactic acid bacteria (LAB) are a group of gram-positive microorganisms capable of fermenting carbohydrates into lactic acid. This study was conducted using a systematic method. The observation process was carried out through a selection process of selected reporting items for systematic reviews and meta-analyses (prism). Formation of lactic acid bacteria with steps of lactic acid isolation, genomic DNA extraction, gene amplification, agarose gel electrophoresis, DNA purification and sequencing, bioinformatics analysis and phylogenetic analysis. The effectiveness of lactic acid bacteria isolation from milk includes phenotypic ambiguity (low taxonomic resolution), long analysis time and labor intensity. The transformation from phenotypic to molecular identification includes high precision (high taxonomic resolution), phylogenetic analysis and global standardization, efficiency, stability, and reproducibility. The potential of lactic acid bacteria as functional food agents includes: resistance to the digestive tract, antimicrobial activity, probiotic characteristics and safety (GRAS). The conclusion is that molecular identification of lactic acid bacteria makes isolation and identification effective and high precision. Isolating lactic acid bacteria as functional foods, namely resistance to the digestive tract, antimicrobial activity, probiotic characteristics and safety (GRAS).
Co-Authors Agam Setyoadi Ahmad Sobingin Aji, Bayu Laksono Almadani, Hanif Anas Malik Taufiqurrohman Budi Setiawan Duwik Istifani Dwi Septi Fatinah Dwi Septi Fatinah Faruq Iskandar Faruq Iskandar Fauzi Ahmad Muda Hanung Dhidhik Arifin Hanung Dhidhik Arifin Iskandar, Faruq Jeki M.W. Wibawanti Jeki Wediantari Wahyu Wibawanti Lailatus Sa’adah Lukman Fadhiliya Mardhianto, Mardhianto Mudawaroch, Roisu Eny Mustofa Kamal Khoerudin Nadia Maharani Najib, Muhammad Sufyan Ngatman, Tri Nur Azizah Nur Azizah Pamungkas, Sapto Priyono Priyono Rini Lestari Robidin, Zaenal Roisu E.M Roisu Eny Mudawaroch Rubiyanti, Novi Sapto Pamungkas Sa’adah, Lailatus Sedya, Iga Wahyuning Syafira Fitria Putri Tri Ngatman Wibawanti, Jeki Mediantari Wahyu Zulfanita Zulfanita