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Karakterisasi Bakteri Asam Laktat dan Total Asam Organik Ekoenzim Asal Filtrat Feses Sapi Potong dan Jerami Padi Pada Lama Fermentasi yang Berbeda Sari, Aisa Hafidah; Marlina, Eulis Tanti; Hidayati, Yuli Astuti
Jurnal Teknologi Hasil Peternakan Vol 5, No 1 (2024): Maret
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v5i1.54289

Abstract

The objective of the research is to identify the effect of fermentation time on ecoenzymes from a mixture of beef cattle feces and rice straw on amount of lactic acid bacteria and total organic acids. The filtrate was from a mixture of beef cattle feces and rice straw with a C/N of 30 and decomposed for 7 days. The filtrate is mixed with molasses and water, fermented by facultative anaerobes. The research method used a Completely Randomized Design (CRD) with 4 treatments fermentation duration, P1=7 days, P2=14 days, P3=21 days, P4=28 days, with 5 repetitions. The parameters measured are amount of lactic acid bacteria, total organic acids, and macroscopic and microscopic characterization of lactic acid bacteria. Data were analyzed using the Analysis of Variance (ANOVA) method with Tukey's advanced test. The number of lactic acid bacteria and total organic acids with the highest value was obtained at a fermentation time of 7 days. Macroscopic characterization of lactic acid bacteria is white and cream in color, round shape, convex elevation, smooth edges and shiny surface, with microscopic characteristics is gram-positive bacteria with a bacil shape.
Dinamika Populasi dan Identifikasi Bakteri pada Proses Dekomposisi Awal Campuran Lumpur Susu dan Jerami Padi dengan Perbedaan Nisbah C/N Kurniawan, Endang; Badruzzaman, Deden Zamzam; Marlina, Eulis Tanti
Jurnal Teknologi Hasil Peternakan Vol 4, No 2 (2023): September
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v4i2.47920

Abstract

Milk sludge has the potential to be converted into compost with the addition of rice straw as a counterbalance in carbon and nitrogen content for a source of microorganism nutrition. Early decomposition is the highest phase of microorganism growth. Microorganisms that play a dominant role are bacteria. This study aims to determine the number, population dynamics, characteristics of bacteria in the initial decomposition process of a mixture of milk sludge and rice straw with differences in the ratio of C / N. The study was conducted for 7 days with experimental and descriptive methods with 3 treatments (P1 = C/N 25, P2 = C/N 27,5, and P3 = C/N 30) and random sampling by sampling. Data on the number of bacterial populations were analyzed using ANOVA while population dynamics and bacterial characteristics used descriptive methods. The number of bacterial populations of each treatment is not influenced by different C/N ratios and has a range of P1 ranging from 0,49 x 10 12 to 27,93 x 10 12 cfu/gram, P2 ranging from 0,39 x 10 12 to 19,32 x 10 12 cfu/gram, and P3 ranging from 0,95 x 10 12 to 10,55 x 10 12 cfu/gram. The population dynamics of bacteria are sigmoid and occur due to temperature fluctuations in the decomposition process. The macroscopic characteristics of each treatment have the same relative shape including filamentous, irregular, circular, rhizoid and white in color and differ on surface and edges. While the microscopic characteristics of bacteria are dominated by rod cell shapes and are gram-positive.
Kualitas Mikroba Produk Olahan Daging yang Dijual Secara Daring Dari UMKM di Kota Bandung Maulidina, Rizka; Marlina, Eulis Tanti; Utama, Dicky Tri
Jurnal Teknologi Hasil Peternakan Vol 4, No 2 (2023): September
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v4i2.47313

Abstract

Culinary is one of the choices of business fields that are in great demand by Micro, Small and Medium Enterprises (MSMEs) entrepreneurs in starting their business. E-commerce can be used by MSME entrepreneurs to distribute products to consumers in the digital transformation phase. The study was conducted to examine the microbiological quality of processed meat products sold online from the city of Bandung and sent throughout Indonesia. The research method used is descriptive with a non-experimental quantitative approach to describe the results of total bacterial analysis using the Total Plate Count (TPC) method and total coliform analysis using the Most Probable Number MPN) method. The sampling technique uses purposive sampling on Shopee and Tokopedia online sales media based on regular delivery methods. Samples of processed meat products in the form of meatballs and nuggets. The research data were analyzed using descriptive statistics which are mean, standard deviation and coefficient of variation. The results showed an average total number of bacteria in processed meat products in the form of meatballs as much as 2.10 x 10 10 CFU g and nuggets as much as 4.29 x 10⁷ CFU/g. The total number of meatball coliforms is 43 to >1100 MPN/g while nuggets are 1100 to >1100 MPN/g. The results obtained from this study showed that the total bacteria and coliforms in processed meat products exceeded the threshold specified in SNI 7388:2009, which are 10 5 cfu/g for total bacteria and 10 MPN/g for total coliform
Analisis Kandungan Boraks dan Formalin pada Produk Olahan Daging yang dijual oleh UMKM di Kota Bandung Alifia, Nabila Nissa; Marlina, Eulis Tanti; Utama, Dicky Tri
Jurnal Teknologi Hasil Peternakan Vol 4, No 1 (2023): Maret
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v4i1.46403

Abstract

Beef meatballs and chicken nuggets are popular processed meat products that are in great demand. Currently, many micro, small, and medium enterprises (MSME) produce beef meatballs and chicken nuggets and then sell them through online e-commerce. Bandung City is one of the cities with 121,530 (26.17%) out of 464,346 MSMEs in food sector. This study aims to evaluate the food safety of beef meatballs and chicken nuggets produced by MSMEs in Bandung City with online sales to various cities in Indonesia. The content of sodium borate (borax) and formaldehyde, which in many cases are added to replace legal food additives to beef meatballs and chicken nuggets. The research was conducted descriptively with qualitative and quantitative methods. The number of samples was 20 products (14 beef meatball products and 6 chicken nugget products) from 20 different MSMEs in Bandung City. The turmeric paper method was used for detecting borax qualitatively, while the KMnO4 reagent method was used for detecting formaldehyde. Positive results were quantitatively tested using spectro-photometer. The results showed that no samples contained borax, but 7 samples (35%) contained formaldehyde, which ranged from 4.16-12.44 ppm. The level of formaldehyde in beef meatballs sold by several MSMEs in Bandung City has violated the rules set in Indonesia.
Pengaruh Lama Fermentasi Ekoenzim Asal Campuran Feses Sapi Potong Dan Jerami Padi Terhadap Total Bakteri, Kadar Alkohol dan pH Arwadani, Iswari; Marlina, Eulis Tanti; Hidayati, Yuli Astuti
Jurnal Teknologi Hasil Peternakan Vol 5, No 1 (2024): Maret
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v5i1.53865

Abstract

The increase in waste volume from the livestock and agriculture sectors requires effective management strategies, including the utilization of eco-enzyme production. Eco-enzymes are versatile liquids made from organic waste, sugar, and water. Generally, eco-enzymes are produced from fruit and vegetable waste; however, evolving information indicates that eco-enzymes can be derived from various types of waste, including livestock waste. The objective of this research is to evaluate the impact of fermentation duration and determine the optimal fermentation period for eco-enzymes derived from a mixture of beef cattle feces and rice straw, specifically focusing on total bacteria, alcohol content, and pH. The research method used was experimental, employing a Completely Randomized Design (CRD), with One-way Anova and Tukey test as the statistical analyses. Four fermentation duration treatments were examined: P1 (7 days), P2 (14 days), P3 (21 days), and P4 (28 days), each replicated five times. The results showed that fermentation duration significantly influenced the pH value, but had no effect on total bacteria and alcohol content. A 28-day fermentation period was identified as the optimal duration for producing eco-enzymes with the highest quality, with the lowest pH reaching 3.45
Identifikasi Bakteri dan Kapang dalam Proses Pembuatan Bioetanol Menggunakan Campuran Feses Sapi Perah dan Tandan Kosong Kelapa Sawit Riyanto, Kirana Fairuza; Marlina, Eulis Tanti; Harlia, Ellin
Jurnal Teknologi Hasil Peternakan Vol 5, No 2 (2024): September
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v5i2.54748

Abstract

Empty palm bunches are plantation waste with a high fibre content that has potential as a bioethanol raw material. The high lignin content in TKKS requires decomposition treatment with the addition of dairy cow faeces which acts as a lignin degrader due to the presence of cellulolytic bacteria. The initial decomposition phase is the phase where microorganisms play a role in degrading cellulose substrates into glucose needed in the bioethanol fermentation process. This study aims to determine the population and characteristics of bacteria and moulds that play a role in the process of making bioethanol using a mixture of dairy cow faeces and EFB  macroscopically and microscopically. This study used descriptive analysis with 4 treatments namely P1 (60% Dairy Cow Feces : 40% EFB), P2 (70% Dairy Cow Feces : 30% EFB), P3 (80% Dairy Cow Feces : 20% EFB), and P4 (90% Dairy Cow Feces : 10% EFB) observations on day 1, 7 and 14 of the initial decomposition phase. The results showed that the highest bacterial population was 11,35 CFU/g in the P1 treatment and the highest mould population was 60 CFU/g. in the P4 treatment. The dominant bacteria are rod-shaped, gram-positive bacteria and the genus Bacillus bacteria. The moulds that play a role in the decomposition process of the mixture of TKKS and dairy cow faeces are Aspergillus, Trichoderma, Penicillium, Rhizopus and Mucor.
Penyuluhan dan Pelatihan Pengolahan Limbah Organik Rumah Tangga pada Kader PKK di Desa Padasuka, Kecamatan Cimahi Tengah, Kota Cimahi Hidayati, Yuli Astuti; Marlina, Eulis Tanti; Harlia, Ellin; Badruzzaman, Deden Zamzam
Farmers: Journal of Community Services Vol 5, No 1 (2024)
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v5i1.52322

Abstract

Penumpukan sampah dapat diatasi dengan mengolah limbah menggunakan metode pengomposan, melalui kegiatan pengabdian kepada masyarakat, telah dilaksanakan kegiatan penyuluhan dan demonstrasi tentang pengolahan limbah organik rumahtangga dengan cara pengomposan. Peserta pelatihan adalah ibu-ibu kader PKK. Tujuan dari kegiatan ini adalah memberikan penyuluhan dan pelatihan tentang pengolahan limbah organik rumahtangga dengan proses pengomposan yang menghasilkan kompos dan kompos ini dapat dimanfaatkan sebagai pupuk bagi tanaman disekitar rumahtangga tertebut dan pada akhirnya akan melestarikan lingkungan. Hasil identifikasi dan analisis pengetahuan tentang limbah anorganik dan limbah organic (92 %). jumlah limbah yang dihasilkan (8 %). limbah organik harus diolah agar tidak berpotensi mencemari lingkungan, (32 - 56 %). Peserta yang sudah mengolah limbah organik rumahtangga hanya sebagian kecil ( 16 %), cara mengolah limbah organik rumahtangga 16 %, proses pengolahan limbah organik rumahtangga dengan cara pengomposan 16 %, persyaratan yang harus dipenuhi untuk proses pengomposan, 8 %. proses pengomposan sudah berakhir 8 %. hasil pengomposan berupa kompos 56 %. kompos dapat bermanfaat sebagai pupuk tanaman 100%, memanfaatkan kompos sebagai pupuk untuk tanaman 80 %, setelah mengikuti penyuluhan dan pelatihan pengetahuan tersebut 100 % dapat dimengerti.
Identifikasi Bakteri dan Kapang dalam Proses Pembuatan Bioetanol Menggunakan Campuran Feses Ayam Broiler dan Tandan Kosong Kelapa Sawit Assidiqi, Hadi Yusril; Marlina, Eulis Tanti; Harlia, Ellin
Madani: Jurnal Ilmiah Multidisiplin Vol 2, No 8 (2024): Vol. 2, No. 8, 2024
Publisher : Penerbit Yayasan Daarul Huda Kruengmane

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.13294645

Abstract

Every year, waste from the livestock and agricultural sectors increases, including  broiler chicken feces and empty fruit bunches  (EFB). EFB contains 32.57% cellulose, 27.70% hemicellulose, and 26.49%. The presence of lignin in lignocellulose is an obstacle, so it is necessary to degrade it with the help of cellulolytic bacteria in broiler chicken feces through the decomposition stages. This research aims  to identify macroscopically and microscopically the bacteria and mold in making bioethanol using a mixture of broiler chicken feces and EFB.  This research used an exploratory method, the data obtained was then analyzed descriptively with 3 treatments and 6 replications for each treatment, namely P1 (60% broiler chicken feces  : 40% EFB), P2 (70% broiler chicken faces : 30% EFB), and P3 (80% broiler chicken feces  : 20% EFB) which were observed on days 1, 7, and 14 in the decomposition stage. The highest average colony of bacteria was 199.83 x 108 CFU/gram and mold was 106.17  x 104 CFU/gram. The Bacillus group was the most commonly found bacterial group,  with 11 isolates from 27 isolates. Aspergillus, Trichoderma, and Penicillium   are the mold groups that play a role in this research.
IDENTIFIKASI BAKTERI DAN KAPANG PADA PROSES PRE-TREATMENT FESES SAPI POTONG DAN TANDAN KOSONG KELAPA SAWIT Safitry, Rania Zabina; Marlina, Eulis Tanti; Harlia, Ellin
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 49, No 3 (2024)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v49i3.15050

Abstract

The abundance of livestock and agricultural waste needs special attention, especially in its processing. One of the largest wastes generated from the livestock sub-sector is beef cattle feces; in the agricultural sub-sector, empty fruit bunches are produced (EFB). In this study, beef cattle feces and empty fruit bunches were decomposed for 14 days to degrade organic matter into raw materials for second-generation bioethanol. This study aimed to identify bacteria and molds that play a role in the decomposition of beef cattle feces and EFB. The research conducted used a qualitative-descriptive method. This study identified microorganisms in the biological pre-treatment process on days 1st, 7th, and 14th. The identification of bacteria and molds was done macroscopically and microscopically. The genus of bacteria found in biological pre-treatment is Bacillus. There are 3 genera of molds found in biological pre-treatment including Aspergillus, Trichoderma, and Mucor. These genera have cellulotic and lignolytic potential in fiber degradation in biological pre-treatment.
ISOLASI TOTAL BAKTERI DAN KOLIFORM PADA MEDIA PERTUMBUHAN MAGGOT DARI FESES SAPI POTONG DAN LIMBAH PASAR Wahyuni, Sri; Badruzzaman, Deden Zamzam; Marlina, Eulis Tanti; Hidayati, Yuli Astuti
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 50, No 1 (2025)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v50i1.18128

Abstract

Produksi limbah organik seperti feses sapi potong dan limbah pasar cukup banyak dan jika tidak dikelola dengan baik akan menyebabkan dampak negatif terhadap lingkungan. Oleh karena itu, metode alternatif untuk mengatasi permasalahan tersebut yaitu dengan memanfaatkan detritivora maggot BSF yang mampu menguraikan bahan organik dengan cepat. Penelitian ini bertujuan untuk mengetahui total bakteri dan koliform pada media pertumbuhan maggot BSF. Penelitian ini dilakukan secara eksperimental dengan 3 perlakuan dan 6 ulangan. Perlakuan yang digunakan dalam penelitian ini yaitu P1= media 100% feses sapi potong, P2= media 100% limbah pasar, P3= media kombinasi 50% feses sapi potong  dan 50% limbah pasar. Data di analisis secara deskriptif. Hasil penelitian yang diperoleh menunjukkan total bakteri setelah diinkubasi 15 hari mengalami penurunan pada P1=1,83 x 1010 CFU/g, P2= 7,16 x 1010 CFU/g dan P3= 1,16 x 1010 CFU/g. Sedangkan hasil total koliform pada P1= 14,83 x 106 CFU/g, P2= 8,50 x 106 CFU/g dan P3= 1,16 x 106 CFU/g.