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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences ILMU KELAUTAN: Indonesian Journal of Marine Sciences AL KAUNIYAH Journal of Science and Applicative Technology Jurnal SOLMA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal BIOMA : Jurnal Biologi dan Pembelajarannya Gunung Djati Conference Series
Yanti Ariyanti
Biology Study Program, Institut Teknologi Sumatera
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Other

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DNA Barcoding for Selected Mangrove-Based Estuary Fishes from Way Kambas National Park, Lampung Province, Indonesia Yanti Ariyanti; Ika Agus Rini; Indah Oktaviani; Sovia Santi Leksikowati
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.04

Abstract

Over the past decade, DNA barcoding has provided new insight into fish ecology and biosystematics and led to new species' discovery. DNA barcoding is a method for the recognition and identification of species using short, standardised DNA fragments. The correct taxonomic identification of species is critical for the assessment and monitoring of biodiversity. This study applied DNA barcoding techniques to identify selected fish species from a mangrove-based estuary in Way Kambas National Park, Lampung Province, Indonesia. The gene encoding cytochrome c oxidase subunit I (COI) was amplified and bi-directionally sequenced from 22 specimens. The resulting 680 base pairs (bp) sequence was used to identify species, obtain phylogenetic information, and analyse genetic distances. A neighbour-joining tree was constructed based on the mitochondrial COI gene using the Kimura two-parameter model. This study also exhibits conservation status for those identified species. Our findings will facilitate future studies of fish species diversity in mangrove estuary-based ecosystems and provide preliminary data in policymaking in conservation areas such as National Park.
Co-Authors ACHMAD FARAJALLAH Fidya Septiana Gres Maretta, Gres Hendry Wijayanti Ika Agus Rini Ika Agus RINI JAYANI, FARADILA MEI Kurnia Wahyu Leksikowati, Sovia Santi Muhammad Asril Novriadi Novriadi Novriadi Oktaviani, Indah Sister Sianturi Tarra Ivanka Winati Nurhayu Yeni Rahayu