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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal FishtecH Jurnal Pendidikan Teknologi Pertanian Seshiski: Southeast Journal of Language and Literary Studies Biodiversitas Journal of Biological Diversity
Asmariani Asmariani
Fisheries Laboratory, Faculty Of Fisheries And Marine Science, Universitas Halu Oleo, Jl. HEA. Mokodompit, Kampus Hijau Bumi Tridharma, Anduonohu, Kendari 93232, Southeast Sulawesi, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Education Immunology & microbiology Languange, Linguistic, Communication & Media Mechanical Engineering Veterinary

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Verifikasi Metode Uji Lemak Pakan Buatan Asmariani Asmariani; Amriani Amriani; Haslianti Haslianti
Jurnal FishtecH Vol 6, No 1 (2017)
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/fishtech.v6i1.4455

Abstract

Verification method is required to generate data analysis with accurate and precise value corresponding to the standrad method. This study aims to verify fat analysis method by using direct extraction method. The study was conducted in Laboratorium Pengujian, FPIK, Universitas Halu Oleo. Fat analysis method verified was direct extraction method or soxhlet method. The results showed that all precision values from experiments I-VIII, consisting of repeatability and reproductibility, have RSD values (0.03 – 0.14) lower than 2/3 RSD Horwitz (1.99). The accuracy results (% Recovery) indicated similarly RSD, lower than 2/3 RSD Horwitz (1.33). Based on these results, it can be concluded that fat analysis method used in Laboratorium Pengujian, meet the pecise and accuray standard, and generates valid data and accounted for.
Pengaruh Penggunaan Sumber Silika Berbeda Terhadap Pertumbuhan dan Produktivitas Biomassa Diatom Skeletonema sp. (Bacillariophyceae) Indrayani Indrayani; Haslianti Haslianti; Asmariani Asmariani; Ardiansyah Ardiansyah
Jurnal Pendidikan Teknologi Pertanian Vol 8, No 2 (2022): Agustus
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/jptp.v8i2.35430

Abstract

Pertumbuhan dan produktivitas biomassa mikroalga khususnya diatom (Bacillariophyceae) sangat dipengaruhi oleh keberadaan silika sebagai penyusun dinding sel (frustule).   Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan silika yang berbeda terhadap pertumbuhan dan produktivitas biomassa mikrolga laut Skeletonema sp. Mikroalga Skeletonema sp. dikultur menggunakan media F/2 dengan sumber silikat yang berbeda yakni Sodium Metasilicate  Powder (Merck), Sodium silicate solution PA (Merck) dan waterglass silikat Teknis pada salinitas 32 ppt, suhu kamar, siklus gelap dan terang 12jam:12 jam selama dua minggu dengan batch mode (in triplicates).  Hasil penelitian menunjukan bahwa Skeletonema sp. tumbuh baik pada semua jenis silika yang digunakan.   Terdapat perbedaan yang signifikan antara laju pertumbuhan spesifik  (LPS) waterglass silika dengan silika solution  (P=0.018) serta antara Sodium silika powder dengan silika solution (P=0.024) namun  tidak terdapat perbedaan yang signifikan antara waterglass silika dan silika powder  (P=0.560).  LPS tertinggi diperoleh pada penggunaan waterglass silika (0,405±0,043d-1) dan terendah pada penggunaan silika solution (0,290±0,037d-1).  Biomass yield antara masing-masing media kultur tidak menunjukkan perbedaan yang signifikan (P=0,770).  Biomass yield berkisar antara 0,60-0,62 g.L-1.  Terdapat perbedaan yang signifikan pada produktivitas biomass antara masing-masing perlakuan (P=0.023).  Produktivitas biomassa tertinggi diperoleh pada penggunaan waterglass silika (0,252±0,033 g.L-1.d-1) dan terendah  pada penggunaan silika solution (0,232±0,015g.L-1.d-1).  Penelitian ini menunjukkan bahwa penggunaan waterglass silika memberikan  pertumbuhan dan produktivitas biomassa tertinggi.  Selain itu waterglass silika jauh lebih murah sehingga lebih ekonomis
EFFECT OF DIFFERENT CULTURE MEDIA ON THE GROWTH AND CHLOROPHYLL-a CONTENT OF Navicula sp. and Synechococcus sp Haslianti; Wa Ode Nilda Arifiana Effendy; Suwarjoyowirayatno; La Ode Baytul Abidin; Muhaimin Hamzah; Asmariani; Fatmawati; Wa Ode Intiyani Mangurana; Herlan Hidayat; Asjun
BIOTROPIA Vol. 33 No. 2 (2026): BIOTROPIA Vol. 33 No. 2 May 2026
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2026.33.2.2708

Abstract

ARTICLE HIGLIGHTS- Walne medium significantly enhances microalgae growth and chlorophyll-a content.- F2+Si medium effectively supports Navicula sp. growth in cultivation.- Urea+NPK limits microalgae due to nutrient deficiency.- Optimal media selection is crucial for tropical microalgae cultivation. ABSTRACTThis research addressed a critical knowledge gap regarding the optimal cultivation conditions for indigenous microalgal strains from high-biodiversity tropical marine ecosystems, offering insights into their physiological responses to varied nutrient regimes. In particular, this study focused on evaluating the effect of three different culture media (Walne, F2+Si, and Urea+NPK) on the growth and chlorophyll-a content of local microalgae isolates, Navicula sp. and Synechococcus sp., isolated from Southeast Sulawesi waters. The isolates were cultivated at a laboratory scale in January 2025 using 250 mL Erlenmeyer flasks containing 100 mL of media, under continuous aeration at room temperature (26 - 30 °C) and constant illumination (90 µmol photons/m²/s). Growth was monitored every two days. The results demonstrated that Walne medium yielded the highest cell density and chlorophyll-a content for both species. In Walne medium, Navicula sp. reached a peak density of 8,656,000 ± 53,777 cells/mL with a specific growth rate (SGR) of 1.1453 ± 0.033, while the density of Synechococcus sp. reached 4,965,833 ± 16,646 cells/mL with an SGR of 0.8297 ± 0.032. The F2+Si medium also supported good growth, particularly for Navicula sp. (SGR of 1.0967 ± 0.003). However, the Urea+NPK medium consistently produced the lowest results for all parameters due to limitations in micronutrient and vitamin. One-Way ANOVA revealed no significant difference (P > 0.05) between Walne and F2+Si media for the growth of Navicula sp., but Walne medium was significantly superior for Synechococcus sp. This study concluded that the choice of the culture medium is crucial for optimizing microalgal growth. Walne medium is recommended as the optimal medium for cultivating both Navicula sp. and Synechococcus sp., whereas F2+Si serves as an effective alternative for Navicula sp. The findings underscore the importance of comprehensive nutrient formulations for maximizing biomass yield in local isolates and provide a foundation for developing cost-effective cultivation strategies in tropical regions.
REGISTER DRIVER MAXIM DALAM GRUP WHATSAPP “PASUKAN ANTI SAMBAT Wa Ode Halfian; Asmariani Asmariani; Ashmarita Ashmarita
Seshiski: Southeast Journal of Language and Literary Studies Vol 5 No 2 (2025): Volume 5 Nomor 2 Desember 2025
Publisher : Himpunan Sarjana Kesusastraan Indonesia, Komisariat Sulawesi Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53922/seshiski.v5i2.96

Abstract

The purpose of this study is to describe the forms of the register used by Maxim drivers in the WhatsApp group “Pasukan Anti Sambat.” The method used in this research is a qualitative descriptive method. This type of research is field research. The data collection process involves reading, observing, taking screenshots, interviewing, and note-taking techniques. The data in this study consist of conversational texts containing registers sourced from the utterances of Maxim drivers in the WhatsApp group Pasukan Anti Sambat. The results of this study indicate that there are 18 registers categorized based on the linguistic units they form, which include (1) single forms, totaling 4 data; (2) complex forms, consisting of 3 data formed through affixation, 2 data formed through compounding, 4 data formed through abbreviation (acronyms), and 3 data formed through shortening; and (3) phrases, totaling 2 data.
Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities Indrayani Indrayani; Haslianti Haslianti; Asmariani Asmariani; Wellem Muskita; Abdul Muis Balubi
Biodiversitas Journal of Biological Diversity Vol. 21 No. 4 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210430

Abstract

Abstract. Indrayani I, Haslianti H, Asmariani A, Muskita WH, Balubi M. 2020. Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities. Biodiversitas 21: 1498-1503. Light is one of the important factors affecting growth and biochemical composition of microalgae. The aim of this study was to determine growth, biomass and lipid productivity of a newly isolated marine diatom, Skeletonema sp.UHO29 under different light intensities. The Skeletonema sp. was cultured in 300 mL conical flasks containing 150 mL of f/2 medium under three different light intensities (28, 60 and 110 ?mol photons m-2 s-1 ) with three replications. The cultures were incubated under ambient room temperatures, and a cycle of 12 hours light and 12 hours dark, in batch modes for two weeks. The results showed that the highest specific growth rate (0.56 d-1) was achieved at the highest light intensity (110 ?mol photons m-2 s-1) and the lowest (0.11 d-1) was obtained at the lowest light intensity (28 ?mol photons m-2 s-1). The highest biomass yield (0.62 g L-1) and biomass productivity (0.34 g L-1 d-1) was achieved at the highest light intensity. However, the highest lipid yield (0.175 g L-1), lipid content (28.78% ash-free dry weight) and lipid productivity (0.067 g L-1 d-1) were achieved at medium light intensity. The alga prefers high light intensity for higher growth rate and biomass productivity but it produces more lipids at medium light intensity. This study indicated that the Skeletonema sp.UHO29 is a potential species for outdoor mass cultivation and is a promising microalgal strain for use as a biodiesel feedstock due to its high growth rate, high biomass and lipid productivity.
Co-Authors Abdul Muis Balubi Amriani Amriani Ardiansyah Ardiansyah Ashmarita, - Asjun Fatmawati Halfian, Wa Ode Hamzah, Muhaimin Haslianti Haslianti, Haslianti Herlan Hidayat Indrayani Indrayani Indrayani Indrayani La Ode Baytul Abidin Mangurana, Wa Ode Intiyani Suwarjoyowirayatno, Suwarjoyowirayatno Waode Nilda Arifiana Effendy Wellem Muskita