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PENGARUH PERBANDINGAN KOSENTRASI GLUKOSA DAN SUKROSA TERHADAP KUALITAS SENSORI DAN KIMIA PERMEN JELLY RUMPUT LAUT (Eucheuma cottonii) Jaldin, Arifi; Haslianti, Haslianti; Asyik, Nur
Jurnal Fish Protech Vol 2, No 2 (2019): Jurnal Fish Protech Vol. 2 No. 2 Oktober 2019
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v2i2.9474

Abstract

The Effect of Comparison of Glucose and Sucrose Concentration on the Sensory and Chemical Quality of Jelly Candy (Eucheuma cottonii) ABSTRACT          The aims of this study were to determine the effect of different concentrations of glucose and sucrose on the sensory and chemical quality of seaweed jelly candy (Eucehuma cottonii). The study used a Completely Randomized Design (CRD) consisting of 4 treatments, namely : P1 (glucose : sucrose  = 45% : 0%), P2 (glucose : sucrose = 0% : 45%, P3 (glucose : sucrose = 30% : 15%), P4 (glucose : sucrose = 15% : 30%) with time three replications. Data from observations were analyzed using ANOVA (Analysis of Variance) at a level of 95%, if there is a real difference (P> 0.05) then a further test was performed with a DMRT (Duncan Multiple Range Test) tests at a 95% significance level. The results obtained show a real effect on sensory values which include color, texture, and taste but no significant effect on the scent. The best results for sensory assessment were found in the P4 treatment which has a taste value of 4.5, texture of 4.2, the scent of 4.0, and a taste of 4.2. The results showed the water content between 22.83%-17.01%, ash content of 0.11%-0.18%. and protein content of 3.75%-5.77%. Keywords: chemistry, glucose, jelly candy, seaweed (Eucheuma cottonii), sensory, and sucroseABSTRAKTujuan penelitian ini adalah untuk mengetahui pengaruh perbedaan kosentrasi glukosa dan sukrosa terhadap kualitas sensori dan  kimia permen jelly rumput laut (E. cottonii). Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan yaitu : P1 (glukosa : sukrosa = 45% : 0%), P2 (glukosa : sukrosa = 0% : 45 %), P3 (glukosa : sukrosa = 30%  : 15%), P4 (glukosa : sukrosa = 15% : 30%) dan ulangan sebanyak tiga kali. Data hasil pengamatan dianalisa menggunakan ANOVA (Analysis of Variance) pada taraf  95%, apabila terdapat beda nyata (P>0,05) maka dilakukan uji lanjut dengan uji DMRT (Duncan Multiple Range Test) pada taraf nyata 95%. Hasil penelitian yang diperoleh menunjukkan pengaruh nyata terhadap nilai sensori yang meliputi warna, tekstur, dan rasa namun diperoleh pengaruh tidak nyata pada aroma. Hasil terbaik untuk penilaian sensori terdapat pada perlakuan P4 yaitu memiliki nilai rasa 4,5, tekstur 4,2, aroma 4,0, dan rasa 4,2. Hasil penelitian menunjukkan kadar air antara 22,83%-17,01%, kadar abu 0,11%-0,18%. dan kadar protein 3,75%-5,77%..Kata kunci: glukosa, kimia, permen jelly, rumput laut (Eucheuma cottonii), sensori, sukrosa
PENGARUH PENAMBAHAN GARAM YANG BERBEDA TERHADAP TINGKAT KESEGARAN IKAN CAKALANG (Katsuwonus pelamis) Mitra, Lia; Haslianti, Haslianti; Suwarjoyowirayatno, Suwarjoyowirayatno
Jurnal Fish Protech Vol 3, No 1 (2020): Jurnal Fish Protech Vol. 3 No. 1 April 2020
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v3i1.11610

Abstract

Effect of Different Additions of Salt on Freshness of Skipjack Fish (Katsuwonus pelamis) ABSTRACT         The aim of this study was to determine organoleptic changes, water content, pH, TPC of skipjack tuna with different handling methods. This study used a Randomized Block Design (RBD) with 2 treatment factors, the first treatment factor of adding salt consisting of 2 levels, 10% salt (P0) and 15% salt (P1). The second treatment factor is storage time which consists of 3 levels, H1 (1 day), H2 (2 days), and H3 (3 days). Organoleptic tests include gills, mucus, eyes, texture and aroma. while chemical tests include water content, pH and TPC. The results of the organoleptic test on the best treatment gill parameter P1H1 with a value of 8,16, mucus with a value of 8.16, eyes with a value of 8,12, aroma with a value of 8,04 and the best treatment texture at P0H1 value of 8,04. While the chemical test of water content was in the P1H1 treatment with a value of 71,74%, the lowest treatment in the P0H1 with a value of 71,16%. The highest value of pH parameters in the P1H1 treatment with a value of 5,84%, the lowest treatment at P0H1 with a value of 5,64%. The highest value of TPC parameters in the P0H1 with a value of 4.67 × 105 CFU/g and the lowest value in the P1H1 with a value of 4.29 × 105 CFU/g. Keywords: addition of salt, the freshness of fish, organoleptic test, skipjack fish, TPC.ABSTRAKTujuan penelitian ini adalah untuk mengetahui perubahan organoleptik, kadar air, pH, TPC ikan cakalang dengan metode penanganan yang berbeda. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 2 faktor perlakuan yaitu faktor perlakuan pertama penambahan garam yang terdiri dari 2 taraf yaitu penambahan garam 10% (P0) dan penambahan garam 15 % (P1). Faktor perlakuan kedua adalah waktu penyimpanan yang terdiri dari 3 taraf yaitu H1 (1 hari), H2 (2 hari), dan H3 (3 hari). Uji organoleptik meliputi insang, lendir, mata, tekstur dan aroma. sedangkan uji kimia meliputi kadar air, pH dan TPC. Hasil penelitian uji organoleptik pada pameter insang perlakuan terbaik P1H1 dengan nilai sebesar 8,16, lendir dengan nilai sebesar 8,16, mata dengan nilai sebesar 8,12,aroma dengan nilai sebesar 8,04 dan tekstur dperlakuan terbaik pada P0H1 nilai sebesar 8,04. Uji kimia nilai tertinggi uji kimia yaitu kadar air terdapat pada perlakuan P1H1 dengan nilai sebesar 71,74%, perlakuan terendah pada perlakuan P0H1 dengan nilai sebesar 71,16%. Parameter pH nilai tertinggi pada perlakuan P1H1 dengan nilai sebesar 5,84%, perlakuan terendah pada P0H1 dengan nilai sebesar 5,64%. Parameter TPC nilai tertinggi pada perlakuan P0H1 dengan nilai sebesar 4,67 × 105 CFU/g dan nilai terendah pada perlakuan P1H1 dengan nilai sebesar 4,29 × 105 CFU/g.Kata kunci: Ikan cakalang, kesegaran ikan, penambahan garam, TPC, uji organoleptik.
UJI STABILITAS PENYIMPANAN IKAN PEPEREK (Leiognathus equulus) KERING DENGAN METODE PENGEMASAN YANG BERBEDA Rusnawati, Rusnawati; Haslianti, Haslianti; Suwarjoyowirayatno, Suwarjoyowirayatno
Jurnal Fish Protech Vol 3, No 2 (2020): Jurnal Fish Protech Vol. 3 No. 2 Oktober 2020
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v3i2.15449

Abstract

ABSTRACT         The aim of this study was to determine the storage stability test of dried peperek fish (Leiognathus equulus) with different packaging methods. This study used a randomized block design (RBD) consisting of 2 treatment factors. The first treatment factor is PE plastic packaging (VO) and aluminum foil plastic packaging (V1). The second treatment factor is storage time which consists of 4 levels, namely H1 (1 day), H2 (7 days), H3 (14 days), and H4 (21 days). Test parameters include organoleptic tests (appearance, aroma, and texture), TPC, and moisture content. Based on the results, it showed that the effect of storage time of PE plastic packaging and aluminum foil plastic packaging on organoleptic tests is appearance V0T14 8.32, V1H1 6.64, aroma V0T14 8.36, V1H1  3.69, texture V0T14 8.52,  V1H1 4. while the best moisture content test results are found in V1H14 with the lowest value of 13.94% and lowest TPC content in V1H14 with the lowest value of 1.46x105. Keywords: fish peperek (leiognathuus equlus), Total Plate Count TPC, water content test, and organolepticABSTRAKPenelitian ini bertujuan untuk mengetahui uji stabilitas penyimpanan ikan peperek (Leiognathus equulus) kering dengan metode pengemasan yang berbeda. Penelitian ini menggunakan rancangan acak kelompok (RAK) yang terdiri dari 2 faktor perlakuan. Faktor perlakuan pertama yaitu kemasan plastik pe (VO) dan kemasan plastik aluminium foil (V1). Faktor perlakuan kedua adalah waktu penyimpanan yang terdiri dari 4 taraf yaitu H1 (1 hari), H2 (7 hari), H3 (14 hari) dan H4 (21 hari). Parameter uji meliputi Uji organoleptik (kenampakan, aroma, dan tekstur), TPC dan kadar air. Berdasarkan hasil penelitian, menunjukkan bahwa pengaruh lama penyimpanan kemasan plastik PE dan kemasan plastik aluminium foil terhadap uji organoleptik yaitu kenampakan V0T14 8.32, V1H1 6.64, aroma V0T14 8.36, V1H1  3.69 tekstur V0T14 8.52,  V1H1 4. sedangkan hasil uji kadar air  terbaik terdapat pada V1H14 dengan nilai terendah 13.94%, dan kandungan TPC terendah V1H14 dengan nilai terendah 1.46x105.Kata kunci: Ikan peperek (leiognathuus equlus), Total Plate Count TPC, uji kadar air, dan uji organoleptik
PENGARUH PENAMBAHAN RUMPUT LAUT (Eucheuma cottonii) TERHADAP KUALITAS SENSORIK DAN PROKSIMAT PUDING Syafar, Ahmad; Haslianti, Haslianti; Asyik, Nur
Jurnal Fish Protech Vol 2, No 2 (2019): Jurnal Fish Protech Vol. 2 No. 2 Oktober 2019
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v2i2.9475

Abstract

ABSTRACT          The aims of this study are to determine the effect of seaweed (Eucheuma cottonii) and mackerel scad fish (Decapterus spp.) ratio for the organoleptic, physical and chemical test of crackers. This study used a Completely Randomized Design (CRD) which four treatments  P1 (FM 20%: S 5%: TF 75%), P2 (FM 15%: S 10%: TF 75%), P3 (FM 10% : S 15% : TF 75%) and P4 (FM 5%: S 20%: TF 75%) with three times replications. Observation data were analyzed using ANOVA (Analysis of Variance) at the level of 95%, if there were significant differences (P> 0.05) then further testing was carried out by DMRT (Duncan Multiple Range Test) at 95% significance level. The result showed that seaweed and mackerel scad meat ratio had a significant effect on sensory values (aroma, taste, and crispness). The best results for the sensory test were found in P1 where the taste and aroma value was 7.8 and 7.7, while the best result for texture, crispness, and appearance are 7.2, 4.3 and 7.3 was found in P4. The chemical test on crackers for protein, water content and crude fibre was 6,97% - 11,50%, 3,80% - 4,96% and 1,81-4,44% respectively. The physical test of the rising volume shows that P4 had the highest value with 92%. Keywords: Chemical test, Puding, Sea weed (Eucheuma cottonii), sensoryABSTRAKTujuan penelitian ini adalah untuk mengetahui pengaruh penambahan rumput laut terhadap uji sensorik dan uji kimia puding rumput laut (E. cottonii). Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan yaitu : P1 = rumput laut 100g, P2= rumput laut 125g, P3=  rumput laut 150g, P4= rumput laut 200g dan ulangan sebanyak tiga kali. Data hasil pengamatan dianalisa menggunakan ANOVA (Analysis of Variance) pada taraf  95%, apabila terdapat berpengaruh nyata (P>0,05) maka dilakukan uji lanjut dengan uji DMRT (Duncan Multiple Range Test) pada taraf nyata 95%. Hasil penelitian menunjukkan pengaruh sangat nyata terhadap nilai sensori meliputi warna dan tekstur sedangkan rasa berpengaruh sangat nyata namun diperoleh pengaruh tidak nyata pada aroma.  Hasil terbaik untuk penilaian sensori terdapat pada perlakuan P4 yaitu dimana memiliki nilai rasa 4,26, tekstur 5,49, aroma 4,4, dan rasa 4,5. Hasil penelitian menunjukkan kadar air antara 74,24%-68,49%, kadar abu 3,44%-3,30%, dan kadar protein 0,108%-0,133%.Kata kunci: Puding, Rumput laut (Eucheuma cottonii), sensori, Uji kimia
PENGARUH PENGGUNAAN ASAM ASAM ASETAT, ASAM KLORIDA DAN ASAM SITRAT TERHADAP KARAKTERISTIK LEM DARI LIMBAH SISIK IKAN KAKAP MERAH (Lutjanus argentimaculatus) Mariani, Mariani; Haslianti, Haslianti; Suwarjoyowirayatno, Suwarjoyowirayatno
Jurnal Fish Protech Vol 3, No 2 (2020): Jurnal Fish Protech Vol. 3 No. 2 Oktober 2020
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v3i2.15450

Abstract

ABSTRACT         The aim of this study was to determine the effect of acetic acid, hydrochloric acid, and citric acid on the physical characteristics of glue from red snapper scales. This study used a completely randomized design (CRD) with one factor of the acid solvent type (5% acetic acid, 5% hydrochloric acid, and 5% citric acid with 150 g of fish scales. Observation data were analyzed using ANOVA (Analysis of Variance) at the 95% level, if there is a significant difference (p <0.05) then a further test is carried out with the DMRT (Duncan Multiple Range Test) tests at the 95% significant level. The results obtained showed that the effect of acetic acid, hydrochloric acid, and citric acid on the characteristics of glue from red snapper fish scales had a significant effect on the adhesive strength value of fish glue, wood surface damage, viscosity, water content but not the pH of fish glue. The best results for the stickiness of fish glue, wood surface damage, fish glue viscosity, pH, and water content were obtained on extraction using acetic acid 6.61; 40.75; 3.60; 4.46; and 48,26 respectively. The results obtained showed that the effect of using acetic acid, hydrochloric acid, and citric acid on the characteristics of glue from red snapper fish scales had a significant effect on the adhesive strength value of fish glue, wood surface damage, viscosity, water content but not the pH of fish glue. Keywords: Acetic acid, citric acid,  fish glue, fish scales, hydrochloric acid.ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penambahan asam asetat, asam klorida, dan asam sitrat terhadap karakteristik fisik lem dari sisik ikan kakap merah dan untuk mengetahui pengaruh penambahan asam asetat, asam klorida dan asam sitrat terhadap karakteristik kimia lem dari sisik ikan kakap merah. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu jenis pelarut asam (asam asetat 5%, asam klorida 5%, dan asam sitrat 5% dengan sisik ikan sebanyak 150 g. Data hasil pengamatan dianalisa menggunakan ANOVA (Analysis of Variance) pada taraf 95%, apabila terdapat beda nyata (p<0,05) maka dilakukan uji lanjut dengan uji DMRT (Duncan Multiple Range Test) pada taraf nyata 95%. Hasil penelitian yang diperoleh menunjukkan bahwa pengaruh penggunaan asam asetat, asam klorida dan asam sitrat terhadap karakteristik lem dari limbah sisik ikan kakap merah memberikan pengaruh nyata terhadap nilai keteguhan rekat lem ikan, kerusakan permukaan kayu, viskositas, kadar air namun tidak dengan pH lem ikan. Hasil terbaik untuk uji keteguhan rekat lem ikan, kerusakan permukaan kayu, viskositas, pH lem ikan dan kadar air lem ikan terdapat pada ekstraksi menggunakan asam asetat dengan nilai masing-masing 6.61; 40.75; 3.60; 4.46 dan 48.26.Kata kunci: Asam asetat, asam klorida, asam sitrat, lem ikan, sisik ikan
KARAKTERISTIK KOMPOSISI KIMIA DAN TOTAL BAKTERI IKAN ROA (Hemirhampus sp.) ASAP YANG DI PASARKAN DI KECAMATAN BUNGKU TENGAH KABUPATEN MOROWALI Sarni, Sarni; Haslianti, Haslianti; Suwarjoyowirayatno, Suwarjoyowirayatno
Jurnal Fish Protech Vol 3, No 1 (2020): Jurnal Fish Protech Vol. 3 No. 1 April 2020
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v3i1.11612

Abstract

Characteristics of Chemical Composition and Total Bacterial of Roa (Hemirhampus sp.) Smoke Marketed in the Bungku District of Central Morowali DistrictABSTRACT         The aim of this study was to determine the characteristics of chemical composition (water, ash, protein, and fat) and total bacteria (TPC) of smoked roa fish. Data analysis this study used descriptive analysis. The method used study is the survey method (Simple Random Sampling). The results of the chemical composition of the highest treatment water content P2 13.02%, ash P3 5.65%, protein P3 40.82%. and fat P3 13.87%. The best organoleptic test results were found in P1 with 7.30 appearance, 8.20 odor, 8.00 taste, 7.80 texture, and the best mushroom specifications were at P2 9.00. The lowest total bacteria test was found with 0,83×105 Log CFU/gr.Keywords: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik ABSTRAKTujuan penelitian ini adalah untuk mengetahui karakteristik komposisi kimia  (kadar air, kadar abu, kadar protein dan kadar lemak) dan total bakteri (TPC) terhadap ikan roa asap. Analisis data yang digunakan dalam penelitian ini yaitu menggunakan analisis deskriptif. Metode yang digunakan dalam penelitian ini yaitu metode survey (Simple Random Sampling). Hasil penelitian komposisi kimia perlakuan tertinggi kadar air P2 13,02%. Kadar abu tertinggi terdapat pada perlakuan P3 5,65%. Kadar protein perlakuan tertinggi terdapat pada P3 40,82%. dan kadar lemak perlakuan tertinggi terdapat pada  P3 13,87%. Hasil penilaian uji organoleptik terbaik terdapat pada P1 sepesifikasi kenampakan 7,30, bau 8,20, rasa 8,00, tekstur 7,80 dan pada spesifikasi jamur nilai terbaik terdapat pada P2 9,00. Total bakteri terendah terdapat pada P3 dengan nilai 0,83×105 Log CFU/gr.Kata kunci: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik
KARAKTERISTIK MUTU SIRUP RUMPUT LAUT (Eucheuma spinosum) Anharullah, La Ode Muhammad; Haslianti, Haslianti; Asyik, Nur
Jurnal Fish Protech Vol 4, No 1 (2021): Jurnal Fish Protech Vol. 4 No. 1 April 2021
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v4i1.18137

Abstract

ABSTRACT         The aim of this study was to determine the quality characteristics of seaweed Syrup (Eucheuma spinosum). This study used a Completely Randomized Design (CRD) consisting of 3 treatments, namely: P1 = 100 gr seaweed: 200 gr sugar: 3.5 L water: CMC 0.3 gr, P2 = 120 gr seaweed: 200 granulated sugar gr: water 3,5 L: CMC 0,3 gr, P3 = Seaweed 140 gr: sugar 200 gr: water 3,5 L: CMC 0,3 gr: with three times replications. Data from observations were analyzed using ANOVA (Analysis of Variance) at a level of 95%, if there is a significant difference (P> 0.05), further tests were carried out with the DMRT (Duncan Multiple Range Test) tests at a 95% confidence level. The results obtained showed that the concentration of seaweed (E. spinosum), water, and taste did not significantly affect the aroma and taste of seaweed syrup. The best treatment was in the treatment of P1 with a scent value of 3.46 with a rather like category, and P1 for a taste of 3.46 with a somewhat like category. The chemical value of seaweed syrup is the value of water content ranging from 38.96% -43.21%, ash content ranges from 0.82-1.34%. Keywords: Syrup, seaweed (Eucheuma spinosum), chemistry and sensoryABSTRAKTujuan penelitian ini adalah untuk mengetahui Karakteristik Mutu Sirup Rumput Laut (Eucheuma spinosum). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan yaitu : P1 = Rumput laut 100 gr : gula pasir 200 gr : air 3,5 L : CMC 0,3 gr, P2 = Rumput laut 120 gr : gula pasir 200 gr: air 3,5 L : CMC 0,3 gr, P3 = Rumput laut 140 gr : gula pasir 200 gr : air 3,5 L : CMC 0,3 gr : dan ulangan sebanyak tiga kali. Data hasil pengamatan dianalisa menggunakan ANOVA (Analysis of Variance) pada taraf 95%, apabila terdapat beda nyata (P>0,05) maka dilakukan uji lanjut dengan uji DMRT (Duncan Multiple Range Test) pada taraf kepercayaan 95%. Hasil penelitian yang diperoleh menunjukkan bahwa Kosentrasi rumput laut (E. Spinosum), air dan rasa berpengaruh tidak nyata terhadap aroma dan rasa sirup rumput laut. Perlakuan terbaik terdapat pada perlakuan P1 dengan nilai aroma sebesar 3,46 dengan kategori  agak suka, dan P1 rasa 3,46 dengan kategori agak suka. Nilai kimia sirup rumput laut yaitu nilai kadar air berkisar 38,96%-43,21%, kadar abu berkisar 0,82-1,34%.Kata kunci: Sirup, rumput laut (Eucheuma spinosum), kimia dan sensori
KARAKTERISTIK KIMIA DAN UJI AKTIVITAS ANTIOKSIDAN RUMPUT LAUT (Sargassum sp.) YANG DIPEROLEH DARI KECAMATAN WANGI-WANGI DAN KECAMATAN WANGI-WANGI SELATAN KABUPATEN WAKATOBI Sarlin, La Ode; Haslianti, Haslianti; Rejeki, Sri
Jurnal Fish Protech Vol 4, No 1 (2021): Jurnal Fish Protech Vol. 4 No. 1 April 2021
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v4i1.18142

Abstract

ABSTRACT         The aim of this study was to determine the proximate content (moisture content, carbohydrate content), phytochemicals (alkaloids, flavonoids, saponins, steroids, terpenoids) and antioxidants. This research used descriptive analysis. Proximate test results on Sargassum sp. P1 = 35.43% and P2 = 34.42% High water content is found in sample code P1, while carbohydrates P1 = 11.85. and P2 = 22.05. Phytochemical test of seaweed Sargassum sp. there are flavonoids, saponins, and terpenoids but there are no alkaloids and steroids. Antioxidant activity of seaweed Sargassum sp. P1 has a value of IC50 = 153.37 while the value of IC50 is at P2 = 162.32. Keywords: Antioxidants, Phytochemicals Sargassum sp, and Proximate.ABSTRAKTujuan penelitian ini untuk mengetahui karakterisasi kimia aktivitas antioksidan rumput laut Sargassum sp. yang diperoleh dari Kecamatan Wangi-Wangi dan Kecamatan Wangi-Wangi selatan Kabupaten Wakatobi.  Penelitian ini menggunakan analisis deskriptif. Hasil Uji proksimat pada rumput laut Sargassum sp. yaitu : P1 = 35,43% dan P2 = 34,42% Kadar air yang tinggi terdapat pada kode sampel P1, Sedangkan karbohidrat dengan kode sampel P1= 11,85. dan P2 = 22,05. Uji fitokimia rumput laut Sargassum sp. terdapat flavonoid, saponin dan terpenoid namun tidak terdapat alkaloid dan steroid. Aktivitas antioksidan rumput laut Sargassum sp. P1 memiliki nilai IC50 = 153,37, sedangkan nilai IC50 pada P2 = 162,32.Kata kunci: Antioksidan, Fitokimia Sargassum sp, dan Proksimat.
UJI FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN DARI RUMPUT LAUT Sargassum sp. DENGAN METODE PENGERINGAN YANG BERBEDA Maulana, Fazirul; Asnani, Asnani; Haslianti, Haslianti
Jurnal Fish Protech Vol 4, No 1 (2021): Jurnal Fish Protech Vol. 4 No. 1 April 2021
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v4i1.18147

Abstract

ABSTRACT         Sargassum sp. is the most abundant type of seaweed from the brown algae group (Phaeophyceae) which is spread in tropical waters. The aim of this study was to determine the phytochemical content and antioxidant activity of the extract of Sargassum sp. with different drying methods. Sargassum sp. dried using room temperature (± 25ºC) for 21 days and oven (40oC) for 30 hours, then extracted using methanol. Phytochemical tests were carried out to determine the presence of alkaloids, tannins, flavonoids, saponins, steroids, and triterpenoids. The antioxidant activity of the seaweed extract of Sargassum sp. tested by the DPPH method (1-1-diphenyl-2-picrylhydrazyl). Phytochemical test results of the extract Sargassum sp. flavonoids, saponins, triterpenoids, and steroids, while alkaloids and tannins were not found. The value of antioxidant activity in the methanol extract of Sargassum sp. shows a higher room temperature treatment with an IC50 value of 823.652 ppm compared to a treatment temperature of 40oC with an IC50 value of 914.1920 ppm. The drying method affects the antioxidant activity of Sargassum sp. Keywords: Sargassum sp., drying, extraction, phytochemicals, antioxidants.ABSTRAKSargassum sp. merupakan jenis rumput laut paling melimpah dari kelompok alga coklat (Phaeophyceae) yang tersebar di perairan tropis. Tujuan penelitian adalah untuk mengetahui kandungan fitokimia dan aktivitas antioksidan ekstrak Sargassum sp. dengan metode pengeringan yang berbeda. Sargassum sp. dikeringkan menggunakan suhu ruang (± 25ºC) selama 21 hari dan oven (suhu 40oC) selama 30 jam, kemudian diekstrak menggunakan pelarut metanol. Uji fitokimia dilakukan untuk mengetahui keberadaan senyawa alkaloid, tanin, flavonoid, saponin, steroid dan triterpenoid pada ekstrak. Aktivitas antioksidan ekstrak rumput laut Sargassum sp. diuji dengan metode DPPH (1-1-difenil-2-pikrilhidrazil). Hasil uji fitokimia pada ekstrak Sargassum sp. yang dikeringkan pada suhu ruang dan suhu 40oC ditemukan adanya flavonoid, saponin, triterpenoid dan steroid sedangkan alkaloid dan tannin tidak ditemukan. Nilai aktivitas antioksidan pada ekstrak metanol Sargassum sp. menunjukan perlakuan suhu ruang lebih tinggi dengan nilai IC50 823,652 ppm dibandingkan perlakuan suhu 40oC dengan nilai IC50 914,1920 ppm. Metode pengeringan berpengaruah terhadap aktivitas antioksidan dari Sargassum sp.Kata kunci: Sargassum sp., pengeringan, ekstraksi, fitokimia, antioksidan.
KARAKTERISTIK KIMIA DAN UJI AKTIVITAS ANTIOKSIDAN RUMPUT LAUT (E. spinosum) YANG DIPEROLEH DARI KECAMATAN WANGI-WANGI SELATAN DAN KECAMATAN KALEDUPA SELATAN Lifendro, Lifendro; Haslianti, Haslianti; Asyik, Nur
Jurnal Fish Protech Vol 4, No 1 (2021): Jurnal Fish Protech Vol. 4 No. 1 April 2021
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v4i1.18138

Abstract

ABSTRACT         The aim of this study was to determine the proximate content (water content, carbohydrate levels), phytochemicals (alkaloids, flavonoids, saponins, steroids, terpenoids), and antioxidants. Data analysis in this study used descriptive analysis to provide a general description of the data. The results of this study aim to determine the content of proximate (water content, carbohydrate levels), phytochemicals (alkaloids, flavonoids, saponins, steroids, terpenoids), and antioxidants. Proximate test on E. spinosum seaweed has a difference, namely water content with sample code P1 = 41.83% and P2 = 49.19. High water content is in the sample code P2, while the carbohydrate content with sample code P1 = 22.20 and P2 = 17.64. High carbohydrate levels are found in the sample code P1. Phytochemical tests on E. spinosum seaweed with codes P1 and P2 contained flavonoids, saponins, and triterpenoids but there were no alkaloids and steroids. The antioxidant activity test on E. spinosum seaweed in both samples was very weak with sample code P1 having IC50 value = 223.90 while IC50 value at P2 = 222.34.Keywords: Antioxidants, Eucheuma spinosum, Phytochemicals and ProximateABSTRAKPeneletian ini bertujuan untuk mengetahui kandungan proksimat (kadar air, kadar karbohidrat), fitokimia (alkaloid, flavonoid, saponin, steroid, terpenoid) dan antioksidan. Analisis data penelitian ini menggunakan analisis deskriptif untuk memberikan gambaran umum tentang data. Hasil uji proksimat pada rumput laut E. spinosum memiliki perbedaan yaitu kadar air dengan kode sampel P1 = 41.83% dan P2 =49.19. Kadar air  tinggi terdapat pada kode sampel P2, sedangkan kadar karbohidrat dengan kode sampel P1 = 22.20 dan P2 = 17.64. Kadar karbohidrat yang tinggi terdapat pada kode sampel P1. Uji fitokimia pada rumput laut E. spinosum dengan kode sampe P1 dan P2  terdapat flavonoid, saponin dan terpenoid namun tidak terdapat alkaloid dan steroid. Uji aktivitas antioksidan pada rumput laut E. spinosum pada kedua sampel sangat lemah dengan kode sampel P1 memiliki nilai IC50 = 223,90 sedangkan nilai IC50 pada P2 = 222,34. Kata kunci: Antioksidan, Eucheuma spinosum, Fitokimia dan Proksimat.
Co-Authors Alam, Yusuf Amriani Amriani Andini Sundowo Anharullah, La Ode Muhammad Aqmal Khaery ARDIANSYAH ARDIANSYAH Ardiansyah Ardiansyah Arsad, Arsad Asmariani Asmariani Asmariani Asmariani Asmariani, Asmariani Asnani Asnani Asriyana Asriyana Baharum, Syarul Nataqain Bana, Sahindomi Bangki, Rafida Baru Sadarun Dedy Oetama Ermayanti Ishak, Ermayanti Fatmawati, Fatmawati Febrianto, Fandi Findra, Muhammad Nur Fristiohady, Adryan Gandri, La Gusti Ayu Kade Sutariati Handrawan, Handrawan Hasuba, Tezza Fauzan Herlan Hidayat Hidayat, Herlan Huli, Laode Ibrahim, Mohamad Nuh Idin, Sahidin INDRAYANI INDRAYANI Indrayani Indrayani Ira Ira, Ira Jaldin, Arifi Jusman Jusman Kamaluddin, La Ode La Ode Aliman Afu La Ode Baytul Abidin La Ode Huli La Ode M. Yasir Haya, La Ode M. La Ode Muhammad Yasir Haya Lawelle, Sjamsu Alam Lifendro, Lifendro Mariani Mariani Maulana, Fazirul Mita Gabriella Inthe Mitra, Lia Muhaimin Hamzah Muhammad Fajar Purnama Muhammad Iqbal Nadia, Laode Muhammad Hazairin Nur Asyik, Nur Nurnaningsih A Permatahati, Yustika Intan Piliana, Wa Ode Rahmadani Rahmadani Ratna Diyah Palupi Rusnawati Rusnawati, Rusnawati Sadimantara, Muhammad Syukri Samsi Burhan Sarlin, La Ode Sarni Sarni Siti Rahmawati Sjamsu Alam Lawelle Suhadar, Ashar Suwarjoyowirayatno Suwarjoyowirayatno Suwarjoyowirayatno, Suwarjoyowirayatno Syafar, Ahmad Syahruddin Syahruddin Tanda, La Tezza Fauzan Hasuba Wa Ode Nila Farlin Wanda Wanda Wiwik Handayani Yakin, Aenul Yodha, Agung Wibawa Mahatva Yundari, Yundari Yustika Permatahati Intan Permatahati Zahra Rahman